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  1. Article ; Online: Extracellular amoebal-vesicles: potential transmission vehicles for respiratory viruses.

    Dey, Rafik / Folkins, Melanie A / Ashbolt, Nicholas J

    NPJ biofilms and microbiomes

    2021  Volume 7, Issue 1, Page(s) 25

    Abstract: Human respiratory syncytial virus (RSV) is a major cause of acute respiratory tract infections in children and immunocompromised adults worldwide. Here we report that amoebae-release respirable-sized vesicles containing high concentrations of infectious ... ...

    Abstract Human respiratory syncytial virus (RSV) is a major cause of acute respiratory tract infections in children and immunocompromised adults worldwide. Here we report that amoebae-release respirable-sized vesicles containing high concentrations of infectious RSV that persisted for the duration of the experiment. Given the ubiquity of amoebae in moist environments, our results suggest that extracellular amoebal-vesicles could contribute to the environmental persistence of respiratory viruses, including potential resistance to disinfection processes and thereby offering novel pathways for viral dissemination and transmission.
    MeSH term(s) Adult ; Amoeba/growth & development ; Amoeba/virology ; Child ; Extracellular Vesicles/virology ; HeLa Cells ; Humans ; Immunocompromised Host ; Models, Biological ; Respiratory Syncytial Virus Infections/transmission ; Respiratory Syncytial Virus, Human/pathogenicity ; Virus Replication
    Language English
    Publishing date 2021-03-17
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2817021-0
    ISSN 2055-5008 ; 2055-5008
    ISSN (online) 2055-5008
    ISSN 2055-5008
    DOI 10.1038/s41522-021-00201-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Interactions between Human Reovirus and Free-Living Amoebae: Implications for Enteric Virus Disinfection and Aquatic Persistence.

    Folkins, Melanie A / Dey, Rafik / Ashbolt, Nicholas J

    Environmental science & technology

    2020  Volume 54, Issue 16, Page(s) 10201–10206

    Abstract: Free-living amoebae (FLA) are ubiquitous protozoa in aquatic/soil habitats and known to resist various disinfection methods commonly used in drinking and wastewater treatment plants. Reoviruses are emerging as useful infectious enteric virus indicators ... ...

    Abstract Free-living amoebae (FLA) are ubiquitous protozoa in aquatic/soil habitats and known to resist various disinfection methods commonly used in drinking and wastewater treatment plants. Reoviruses are emerging as useful infectious enteric virus indicators of wastewater treatment efficacy. The possible enhanced protection FLA may provide reoviruses, however, has not been previously described. Using an infectious clinical reovirus isolate in coculture with three FLA, namely,
    MeSH term(s) Acanthamoeba ; Amoeba ; Disinfection ; Enterovirus ; Humans ; Water Microbiology
    Language English
    Publishing date 2020-07-27
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1520-5851
    ISSN (online) 1520-5851
    DOI 10.1021/acs.est.0c02896
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Interactions between Human Reovirus and Free-Living Amoebae: Implications for Enteric Virus Disinfection and Aquatic Persistence

    Folkins, Melanie A / Dey, Rafik / Ashbolt, Nicholas J

    Environmental science & technology. 2020 July 09, v. 54, no. 16

    2020  

    Abstract: Free-living amoebae (FLA) are ubiquitous protozoa in aquatic/soil habitats and known to resist various disinfection methods commonly used in drinking and wastewater treatment plants. Reoviruses are emerging as useful infectious enteric virus indicators ... ...

    Abstract Free-living amoebae (FLA) are ubiquitous protozoa in aquatic/soil habitats and known to resist various disinfection methods commonly used in drinking and wastewater treatment plants. Reoviruses are emerging as useful infectious enteric virus indicators of wastewater treatment efficacy. The possible enhanced protection FLA may provide reoviruses, however, has not been previously described. Using an infectious clinical reovirus isolate in coculture with three FLA, namely, Vermamoeba vermiformis, Acanthamoeba polyphaga, and Willaertia magna, we followed reovirus persistence (by quantitative reverse transcription polymerase chain reaction (RT-qPCR)) and infectivity (TCID₅₀). Virions present in samples persisted over the experimental time period, with most virions remaining infectious. Surprisingly, electron microscopy revealed virions accumulated within the nucleus of amoebae. The current work appears to be the first report of reovirus being internalized within FLA and remaining infectious, providing a previously unreported environmental reservoir and potential mode of dissemination. FLA also appeared to be providing some logs in protection to internalized viruses during UV irradiation, which if not accounted for when determining UV dosage needed for sufficient disinfection may result in unintentional release of pathogens into surrounding water systems.
    Keywords Acanthamoeba ; Reoviridae ; coculture ; disinfection ; electron microscopy ; humans ; pathogenicity ; reverse transcriptase polymerase chain reaction ; soil ; ultraviolet radiation ; virion ; viruses ; wastewater treatment
    Language English
    Dates of publication 2020-0709
    Size p. 10201-10206.
    Publishing place American Chemical Society
    Document type Article
    Note NAL-AP-2-clean
    ISSN 1520-5851
    DOI 10.1021/acs.est.0c02896
    Database NAL-Catalogue (AGRICOLA)

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    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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