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Article: Clinical Evaluation of the ButterfLife Device for Simultaneous Multiparameter Telemonitoring in Hospital and Home Settings.

Salton, Francesco / Kette, Stefano / Confalonieri, Paola / Fonda, Sergio / Lerda, Selene / Hughes, Michael / Confalonieri, Marco / Ruaro, Barbara

Diagnostics (Basel, Switzerland)

2022 Volume 12, Issue 12

Abstract: We conducted a two-phase study to test the reliability and usability of an all-in-one artificial intelligence-based device (ButterfLife), which allows simultaneous monitoring of five vital signs. The first phase of the study aimed to test the agreement ... ...

Abstract	We conducted a two-phase study to test the reliability and usability of an all-in-one artificial intelligence-based device (ButterfLife), which allows simultaneous monitoring of five vital signs. The first phase of the study aimed to test the agreement between measurements performed with ButterfLife vs. standard of care (SoC) in 42 hospitalized patients affected by acute respiratory failure. In this setting, the greatest discordance between ButterfLife and SoC was in respiratory rate (mean difference -4.69 bpm). Significantly close correlations were observed for all parameters except diastolic blood pressure and oxygen saturation (Spearman's Rho -0.18 mmHg;
Language	English
Publishing date	2022-12-10
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662336-5
ISSN	2075-4418
ISSN	2075-4418
DOI	10.3390/diagnostics12123115
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Article ; Online: Optical and electrical recording of neural activity evoked by graded contrast visual stimulus

Bulf Luca / Salvatori Giorgia / Rovati Luigi / Fonda Sergio

BioMedical Engineering OnLine, Vol 6, Iss 1, p

2007 Volume 28

Abstract: Abstract Background Brain activity has been investigated by several methods with different principles, notably optical ones. Each method may offer information on distinct physiological or pathological aspects of brain function. The ideal instrument to ... ...

Abstract	Abstract Background Brain activity has been investigated by several methods with different principles, notably optical ones. Each method may offer information on distinct physiological or pathological aspects of brain function. The ideal instrument to measure brain activity should include complementary techniques and integrate the resultant information. As a "low cost" approach towards this objective, we combined the well-grounded electroencephalography technique with the newer near infrared spectroscopy methods to investigate human visual function. Methods The article describes an embedded instrumentation combining a continuous-wave near-infrared spectroscopy system and an electroencephalography system to simultaneously monitor functional hemodynamics and electrical activity. Near infrared spectroscopy (NIRS) signal depends on the light absorption spectra of haemoglobin and measures the blood volume and blood oxygenation regulation supporting the neural activity. The NIRS and visual evoked potential (VEP) are concurrently acquired during steady state visual stimulation, at 8 Hz, with a b/w "windmill" pattern, in nine human subjects. The pattern contrast is varied (1%, 10%, 100%) according to a stimulation protocol. Results In this study, we present the measuring system; the results consist in concurrent recordings of hemodynamic changes and evoked potential responses emerging from different contrast levels of a patterned stimulus. The concentration of [HbO2] increases and [HHb] decreases after the onset of the stimulus. Their variation shows a clear relationship with the contrast value: large contrast produce huge difference in concentration, while low contrast provokes small concentration difference. This behaviour is similar to the already known relationship between VEP response amplitude and contrast. Conclusion The simultaneous recording and analysis of NIRS and VEP signals in humans during visual stimulation with a b/w pattern at variable contrast, demonstrates a strong linear correlation between hemodynamic changes and evoked potential amplitude. Furthermore both responses present a logarithmic profile with stimulus contrast.
Keywords	Medicine (General) ; R5-920 ; Medicine ; R ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
Subject code	610
Language	English
Publishing date	2007-07-01T00:00:00Z
Publisher	BioMed Central
Document type	Article ; Online
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Article: The commissural transfer of the horizontal optokinetic signal in the rat: a c-Fos study

Ferrari, Renata / Fonda, Sergio / Corradini, Matteo / Biral, Giampaolo

Experimental brain research. 2009 Sept., v. 198, no. 1

2009

Abstract: We applied the Fos method in rats subjected to horizontal optokinetic stimulation (OKS) to study whether optokinetic information is transferred through the commissural pretectal fibres from one optic tract nucleus (NOT) to the opposite. In binocular as ... ...

Abstract	We applied the Fos method in rats subjected to horizontal optokinetic stimulation (OKS) to study whether optokinetic information is transferred through the commissural pretectal fibres from one optic tract nucleus (NOT) to the opposite. In binocular as well as in monocular nasalward OKS, the highest Fos immunoreactivity was found in the NOT contralateral to the nasalward stimulation, as expression of the activation either of direction-selective cells and of commissural neurons. Even the opposite NOT showed many Fos-positive cells activated by the opposite nucleus throughout the commissural pretectal pathway. They might be the GABA positive cells, which are thought to allow the activation in one nucleus to be transformed into inhibition of the opposite side. In monocular temporalward OKS, the inhibition on direction-selective cells and the consequent silencing of commissural neurons caused the faint immunoreactivity in the NOT contralateral to eye stimulated. In the opposite nucleus the few Fos-positive cells emerged as a consequence of the lack of the normal tonic commissurally mediated inhibition.
Language	English
Dates of publication	2009-09
Size	p. 85-94.
Publisher	Springer-Verlag
Publishing place	Berlin/Heidelberg
Document type	Article
ZDB-ID	1201-4
ISSN	1432-1106 ; 0014-4819
ISSN (online)	1432-1106
ISSN	0014-4819
DOI	10.1007/s00221-009-1935-x
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Article ; Online: The commissural transfer of the horizontal optokinetic signal in the rat: a c-Fos study.

Ferrari, Renata / Fonda, Sergio / Corradini, Matteo / Biral, Giampaolo

Experimental brain research

2009 Volume 198, Issue 1, Page(s) 85–94

Abstract	We applied the Fos method in rats subjected to horizontal optokinetic stimulation (OKS) to study whether optokinetic information is transferred through the commissural pretectal fibres from one optic tract nucleus (NOT) to the opposite. In binocular as well as in monocular nasalward OKS, the highest Fos immunoreactivity was found in the NOT contralateral to the nasalward stimulation, as expression of the activation either of direction-selective cells and of commissural neurons. Even the opposite NOT showed many Fos-positive cells activated by the opposite nucleus throughout the commissural pretectal pathway. They might be the GABA positive cells, which are thought to allow the activation in one nucleus to be transformed into inhibition of the opposite side. In monocular temporalward OKS, the inhibition on direction-selective cells and the consequent silencing of commissural neurons caused the faint immunoreactivity in the NOT contralateral to eye stimulated. In the opposite nucleus the few Fos-positive cells emerged as a consequence of the lack of the normal tonic commissurally mediated inhibition.
MeSH term(s)	Animals ; Biomarkers/metabolism ; Functional Laterality/physiology ; Immunohistochemistry ; Male ; Mesencephalon/anatomy & histology ; Mesencephalon/physiology ; Motion Perception/physiology ; Neural Inhibition/physiology ; Neural Pathways/anatomy & histology ; Neural Pathways/physiology ; Neurons/metabolism ; Neuropsychological Tests ; Nystagmus, Optokinetic/physiology ; Photic Stimulation ; Proto-Oncogene Proteins c-fos/metabolism ; Rats ; Rats, Long-Evans ; Synaptic Transmission/physiology ; Visual Pathways/anatomy & histology ; Visual Pathways/physiology ; gamma-Aminobutyric Acid/metabolism
Chemical Substances	Biomarkers ; Proto-Oncogene Proteins c-fos ; gamma-Aminobutyric Acid (56-12-2)
Language	English
Publishing date	2009-07-17
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1201-4
ISSN	1432-1106 ; 0014-4819
ISSN (online)	1432-1106
ISSN	0014-4819
DOI	10.1007/s00221-009-1935-x
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Article ; Online: Predictive mechanisms in idiom comprehension.

Vespignani, Francesco / Canal, Paolo / Molinaro, Nicola / Fonda, Sergio / Cacciari, Cristina

Journal of cognitive neuroscience

2010 Volume 22, Issue 8, Page(s) 1682–1700

Abstract: Prediction is pervasive in human cognition and plays a central role in language comprehension. At an electrophysiological level, this cognitive function contributes substantially in determining the amplitude of the N400. In fact, the amplitude of the ... ...

Abstract	Prediction is pervasive in human cognition and plays a central role in language comprehension. At an electrophysiological level, this cognitive function contributes substantially in determining the amplitude of the N400. In fact, the amplitude of the N400 to words within a sentence has been shown to depend on how predictable those words are: The more predictable a word, the smaller the N400 elicited. However, predictive processing can be based on different sources of information that allow anticipation of upcoming constituents and integration in context. In this study, we investigated the ERPs elicited during the comprehension of idioms, that is, prefabricated multiword strings stored in semantic memory. When a reader recognizes a string of words as an idiom before the idiom ends, she or he can develop expectations concerning the incoming idiomatic constituents. We hypothesized that the expectations driven by the activation of an idiom might differ from those driven by discourse-based constraints. To this aim, we compared the ERP waveforms elicited by idioms and two literal control conditions. The results showed that, in both cases, the literal conditions exhibited a more negative potential than the idiomatic condition. Our analyses suggest that before idiom recognition the effect is due to modulation of the N400 amplitude, whereas after idiom recognition a P300 for the idiomatic sentence has a fundamental role in the composition of the effect. These results suggest that two distinct predictive mechanisms are at work during language comprehension, based respectively on probabilistic information and on categorical template matching.
MeSH term(s)	Analysis of Variance ; Brain Mapping ; Comprehension/physiology ; Electroencephalography/methods ; Evoked Potentials/physiology ; Female ; Humans ; Male ; Neuropsychological Tests ; Predictive Value of Tests ; Principal Component Analysis ; Reaction Time/physiology ; Recognition (Psychology)/physiology ; Semantics ; Time Factors ; Young Adult
Language	English
Publishing date	2010-08
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1007410-7
ISSN	1530-8898 ; 0898-929X ; 1096-8857
ISSN (online)	1530-8898
ISSN	0898-929X ; 1096-8857
DOI	10.1162/jocn.2009.21293
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Article ; Online: Optical and electrical recording of neural activity evoked by graded contrast visual stimulus.

Rovati, Luigi / Salvatori, Giorgia / Bulf, Luca / Fonda, Sergio

Biomedical engineering online

2007 Volume 6, Page(s) 28

Abstract: Background: Brain activity has been investigated by several methods with different principles, notably optical ones. Each method may offer information on distinct physiological or pathological aspects of brain function. The ideal instrument to measure ... ...

Abstract	Background: Brain activity has been investigated by several methods with different principles, notably optical ones. Each method may offer information on distinct physiological or pathological aspects of brain function. The ideal instrument to measure brain activity should include complementary techniques and integrate the resultant information. As a "low cost" approach towards this objective, we combined the well-grounded electroencephalography technique with the newer near infrared spectroscopy methods to investigate human visual function. Methods: The article describes an embedded instrumentation combining a continuous-wave near-infrared spectroscopy system and an electroencephalography system to simultaneously monitor functional hemodynamics and electrical activity. Near infrared spectroscopy (NIRS) signal depends on the light absorption spectra of haemoglobin and measures the blood volume and blood oxygenation regulation supporting the neural activity. The NIRS and visual evoked potential (VEP) are concurrently acquired during steady state visual stimulation, at 8 Hz, with a b/w "windmill" pattern, in nine human subjects. The pattern contrast is varied (1%, 10%, 100%) according to a stimulation protocol. Results: In this study, we present the measuring system; the results consist in concurrent recordings of hemodynamic changes and evoked potential responses emerging from different contrast levels of a patterned stimulus. The concentration of [HbO2] increases and [HHb] decreases after the onset of the stimulus. Their variation shows a clear relationship with the contrast value: large contrast produce huge difference in concentration, while low contrast provokes small concentration difference. This behaviour is similar to the already known relationship between VEP response amplitude and contrast. Conclusion: The simultaneous recording and analysis of NIRS and VEP signals in humans during visual stimulation with a b/w pattern at variable contrast, demonstrates a strong linear correlation between hemodynamic changes and evoked potential amplitude. Furthermore both responses present a logarithmic profile with stimulus contrast.
MeSH term(s)	Adult ; Brain Mapping/methods ; Contrast Sensitivity/physiology ; Diagnosis, Computer-Assisted/instrumentation ; Diagnosis, Computer-Assisted/methods ; Electroencephalography/instrumentation ; Electroencephalography/methods ; Equipment Design ; Equipment Failure Analysis ; Evoked Potentials, Visual/physiology ; Female ; Humans ; Male ; Middle Aged ; Optics and Photonics/instrumentation ; Reproducibility of Results ; Sensitivity and Specificity ; Signal Processing, Computer-Assisted/instrumentation ; Spectrophotometry, Infrared/instrumentation ; Systems Integration ; Visual Cortex/physiology
Language	English
Publishing date	2007-07-04
Publishing country	England
Document type	Evaluation Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2084374-4
ISSN	1475-925X ; 1475-925X
ISSN (online)	1475-925X
ISSN	1475-925X
DOI	10.1186/1475-925X-6-28
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Article ; Online: Distinct DNA Methylation Profiles in Ovarian Tumors: Opportunities for Novel Biomarkers.

Losi, Lorena / Fonda, Sergio / Saponaro, Sara / Chelbi, Sonia T / Lancellotti, Cesare / Gozzi, Gaia / Alberti, Loredana / Fabbiani, Luca / Botticelli, Laura / Benhattar, Jean

International journal of molecular sciences

2018 Volume 19, Issue 6

Abstract: Aberrant methylation of multiple promoter CpG islands could be related to the biology of ovarian tumors and its determination could help to improve treatment strategies. DNA methylation profiling was performed using the Methylation Ligation-dependent ... ...

Abstract	Aberrant methylation of multiple promoter CpG islands could be related to the biology of ovarian tumors and its determination could help to improve treatment strategies. DNA methylation profiling was performed using the Methylation Ligation-dependent Macroarray (MLM), an array-based analysis. Promoter regions of 41 genes were analyzed in 102 ovarian tumors and 17 normal ovarian samples. An average of 29% of hypermethylated promoter genes was observed in normal ovarian tissues. This percentage increased slightly in serous, endometrioid, and mucinous carcinomas (32%, 34%, and 45%, respectively), but decreased in germ cell tumors (20%). Ovarian tumors had methylation profiles that were more heterogeneous than other epithelial cancers. Unsupervised hierarchical clustering identified four groups that are very close to the histological subtypes of ovarian tumors. Aberrant methylation of three genes (
MeSH term(s)	Biomarkers, Tumor/metabolism ; Cluster Analysis ; DNA Methylation/genetics ; Female ; Humans ; Kaplan-Meier Estimate ; Ovarian Neoplasms/genetics ; Promoter Regions, Genetic
Chemical Substances	Biomarkers, Tumor
Language	English
Publishing date	2018-05-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms19061559
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Article: Cloze probability does not only affect N400 amplitude: the case of complex prepositions.

Molinaro, Nicola / Vespignani, Francesco / Canal, Paolo / Fonda, Sergio / Cacciari, Cristina

Psychophysiology

2008 Volume 45, Issue 6, Page(s) 1008–1012

Abstract: Cloze-probability levels are inversely correlated with N400 amplitude, indicating an easier integration for expected words in semantic-pragmatic contexts. Here we exploited the prespecified standard order of complex prepositions and measured the ERPs ... ...

Abstract	Cloze-probability levels are inversely correlated with N400 amplitude, indicating an easier integration for expected words in semantic-pragmatic contexts. Here we exploited the prespecified standard order of complex prepositions and measured the ERPs time-locked to the last preposition in sentences in which complex prepositions were presented in their standard form or with the last preposition changed. The expected preposition elicited an N280 followed by an N400-700, two ERP components previously associated to the processing of closed-class words. The unexpected preposition elicited only an N280, and the N400-700 was reduced. These results reflect the specificity of the contextual constraints linked to the complex preposition word sequence.
MeSH term(s)	Electroencephalography ; Female ; Humans ; Language ; Male ; Perception/physiology ; Vocabulary ; Young Adult
Language	English
Publishing date	2008-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	209486-1
ISSN	1540-5958 ; 0048-5772
ISSN (online)	1540-5958
ISSN	0048-5772
DOI	10.1111/j.1469-8986.2008.00694.x
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Article: Proteomic and Bioinformatic Studies for the Characterization of Response to Pemetrexed in Platinum Drug Resistant Ovarian Cancer.

Severi, Leda / Losi, Lorena / Fonda, Sergio / Taddia, Laura / Gozzi, Gaia / Marverti, Gaetano / Magni, Fulvio / Chinello, Clizia / Stella, Martina / Sheouli, Jalid / Braicu, Elena I / Genovese, Filippo / Lauriola, Angela / Marraccini, Chiara / Gualandi, Alessandra / D'Arca, Domenico / Ferrari, Stefania / Costi, Maria P

Frontiers in pharmacology

2018 Volume 9, Page(s) 454

Abstract: Proteomics and bioinformatics are a useful combined technology for the characterization of protein expression level and modulation associated with the response to a drug and with its mechanism of action. The folate pathway represents an important target ... ...

Abstract	Proteomics and bioinformatics are a useful combined technology for the characterization of protein expression level and modulation associated with the response to a drug and with its mechanism of action. The folate pathway represents an important target in the anticancer drugs therapy. In the present study, a discovery proteomics approach was applied to tissue samples collected from ovarian cancer patients who relapsed after the first-line carboplatin-based chemotherapy and were treated with pemetrexed (PMX), a known folate pathway targeting drug. The aim of the work is to identify the proteomic profile that can be associated to the response to the PMX treatment in pre-treatement tissue. Statistical metrics of the experimental Mass Spectrometry (MS) data were combined with a knowledge-based approach that included bioinformatics and a literature review through ProteinQuest™ tool, to design a protein set of reference (PSR). The PSR provides feedback for the consistency of MS proteomic data because it includes known validated proteins. A panel of 24 proteins with levels that were significantly different in pre-treatment samples of patients who responded to the therapy vs. the non-responder ones, was identified. The differences of the identified proteins were explained for the patients with different outcomes and the known PMX targets were further validated. The protein panel herein identified is ready for further validation in retrospective clinical trials using a targeted proteomic approach. This study may have a general relevant impact on biomarker application for cancer patients therapy selection.
Language	English
Publishing date	2018-05-08
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2018.00454
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Article ; Online: Conformational Propensity and Biological Studies of Proline Mutated LR Peptides Inhibiting Human Thymidylate Synthase and Ovarian Cancer Cell Growth.

Saxena, Puneet / Severi, Leda / Santucci, Matteo / Taddia, Laura / Ferrari, Stefania / Luciani, Rosaria / Marverti, Gaetano / Marraccini, Chiara / Tondi, Donatella / Mor, Marco / Scalvini, Laura / Vitiello, Simone / Losi, Lorena / Fonda, Sergio / Pacifico, Salvatore / Guerrini, Remo / D'Arca, Domenico / Ponterini, Glauco / Costi, Maria Paola

Journal of medicinal chemistry

2018 Volume 61, Issue 16, Page(s) 7374–7380

Abstract: LR and [d- ... ...

Abstract	LR and [d-Gln
MeSH term(s)	Antineoplastic Agents/chemistry ; Antineoplastic Agents/pharmacology ; Cell Line, Tumor ; Circular Dichroism ; Enzyme Inhibitors/chemistry ; Enzyme Inhibitors/pharmacology ; Female ; Humans ; Molecular Dynamics Simulation ; Mutation ; Ovarian Neoplasms/drug therapy ; Ovarian Neoplasms/pathology ; Peptides/chemistry ; Peptides/genetics ; Peptides/pharmacology ; Proline/genetics ; Protein Conformation ; Thymidylate Synthase/antagonists & inhibitors ; Thymidylate Synthase/genetics ; Thymidylate Synthase/metabolism
Chemical Substances	Antineoplastic Agents ; Enzyme Inhibitors ; Peptides ; Proline (9DLQ4CIU6V) ; Thymidylate Synthase (EC 2.1.1.45)
Language	English
Publishing date	2018-08-13
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	218133-2
ISSN	1520-4804 ; 0022-2623
ISSN (online)	1520-4804
ISSN	0022-2623
DOI	10.1021/acs.jmedchem.7b01699
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