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  1. Article ; Online: Alternate typhoid toxin assembly evolved independently in the two

    Chemello, Antonio J / Fowler, Casey C

    mBio

    2024  Volume 15, Issue 4, Page(s) e0340323

    Abstract: AB: Importance: Typhoid toxin is an ... ...

    Abstract AB
    Importance: Typhoid toxin is an important
    MeSH term(s) Humans ; Typhoid Fever/microbiology ; Bacterial Toxins/genetics ; Bacterial Toxins/metabolism ; Salmonella/metabolism ; Salmonella typhi/genetics ; Virulence Factors/genetics ; Virulence Factors/metabolism
    Chemical Substances Bacterial Toxins ; Virulence Factors
    Language English
    Publishing date 2024-03-19
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2557172-2
    ISSN 2150-7511 ; 2161-2129
    ISSN (online) 2150-7511
    ISSN 2161-2129
    DOI 10.1128/mbio.03403-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Conserved patterns of sequence diversification provide insight into the evolution of two-component systems in

    Barretto, Luke A F / Van, Patryc-Khang T / Fowler, Casey C

    Microbial genomics

    2024  Volume 10, Issue 3

    Abstract: Two-component regulatory systems (TCSs) are a major mechanism used by bacteria to sense and respond to their environments. Many of the same TCSs are used by biologically diverse organisms with different regulatory needs, suggesting that the functions of ... ...

    Abstract Two-component regulatory systems (TCSs) are a major mechanism used by bacteria to sense and respond to their environments. Many of the same TCSs are used by biologically diverse organisms with different regulatory needs, suggesting that the functions of TCS must evolve. To explore this topic, we analysed the amino acid sequence divergence patterns of a large set of broadly conserved TCS across different branches of
    MeSH term(s) Klebsiella ; Alleles ; Amino Acid Sequence
    Language English
    Publishing date 2024-03-19
    Publishing country England
    Document type Journal Article
    ZDB-ID 2835258-0
    ISSN 2057-5858 ; 2057-5858
    ISSN (online) 2057-5858
    ISSN 2057-5858
    DOI 10.1099/mgen.0.001215
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Decoding a Salmonella Typhi Regulatory Network that Controls Typhoid Toxin Expression within Human Cells.

    Fowler, Casey C / Galán, Jorge E

    Cell host & microbe

    2018  Volume 23, Issue 1, Page(s) 65–76.e6

    Abstract: Salmonella Typhi is the cause of typhoid fever, a major global health concern. An essential virulence factor of this pathogen is typhoid toxin. In contrast to most AB-type toxins, typhoid toxin is exclusively expressed by intracellular bacteria. The ... ...

    Abstract Salmonella Typhi is the cause of typhoid fever, a major global health concern. An essential virulence factor of this pathogen is typhoid toxin. In contrast to most AB-type toxins, typhoid toxin is exclusively expressed by intracellular bacteria. The regulatory networks that ensure this unique gene expression pattern are unknown. Here, we developed FAST-INSeq, a genome-wide screening approach to identify S. Typhi genes required for typhoid toxin expression within infected cells. We find that typhoid toxin expression is controlled by a silencing and counter-silencing mechanism through the opposing actions of the PhoP/PhoQ two-component regulatory system and the histone-like protein H-NS. The screen also identified bacterial mutants that alter the proportion of intracellular S. Typhi that reside within an intravacuolar environment, which was essential for toxin expression. Collectively, these data describe a regulatory mechanism that allows a bacterial pathogen to exclusively express a virulence factor when located within a specific intracellular compartment.
    MeSH term(s) Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Bacterial Toxins/biosynthesis ; Bacterial Toxins/genetics ; Cell Line, Tumor ; Gene Expression Regulation, Bacterial/genetics ; Gene Regulatory Networks/genetics ; HeLa Cells ; Humans ; Salmonella typhi/genetics ; Salmonella typhi/pathogenicity
    Chemical Substances Bacterial Proteins ; Bacterial Toxins ; PhoQ protein, Bacteria ; PhoP protein, Bacteria (125360-99-8)
    Language English
    Publishing date 2018-01-04
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2278004-X
    ISSN 1934-6069 ; 1931-3128
    ISSN (online) 1934-6069
    ISSN 1931-3128
    DOI 10.1016/j.chom.2017.12.001
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  4. Article: Identification of A Putative T6SS Immunity Islet in Salmonella Typhi.

    Barretto, Luke A F / Fowler, Casey C

    Pathogens (Basel, Switzerland)

    2020  Volume 9, Issue 7

    Abstract: Typhoid fever is a major global health problem and is the result of systemic infections caused by the human-adapted bacterial ... ...

    Abstract Typhoid fever is a major global health problem and is the result of systemic infections caused by the human-adapted bacterial pathogen
    Language English
    Publishing date 2020-07-11
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens9070559
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  5. Article: The evolutionary diversification of the

    Ojiakor, Adaobi / Gibbs, Rachel N / Chen, Zhe / Gao, Xiang / Fowler, Casey C

    Frontiers in microbiology

    2022  Volume 13, Page(s) 1016438

    Abstract: Salmonella ... ...

    Abstract Salmonella enterica
    Language English
    Publishing date 2022-11-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2022.1016438
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Construction and application of riboswitch-based sensors that detect metabolites within bacterial cells.

    Fowler, Casey C / Li, Yingfu

    Methods in molecular biology (Clifton, N.J.)

    2014  Volume 1103, Page(s) 177–197

    Abstract: A riboswitch is an RNA element that detects the level of a specific metabolite within the cell and regulates the expression of co-transcribed genes. By fusing a riboswitch to a reporter protein in a carefully designed and tested construct, this ability ... ...

    Abstract A riboswitch is an RNA element that detects the level of a specific metabolite within the cell and regulates the expression of co-transcribed genes. By fusing a riboswitch to a reporter protein in a carefully designed and tested construct, this ability can be exploited to create an intracellular sensor that detects the level of a particular small molecule within live bacterial cells. There is a great deal of flexibility in the design of such a sensor and factors such as the molecule to be detected and the downstream experiments in which the sensor will be applied should guide the specific blueprint of the final construct. The completed sensor plasmid needs to be rigorously tested with appropriate controls to ensure that its dynamic range, signal strength, sensitivity and specificity are suitable for its intended applications. In this chapter, methods for the design, assessment and use of riboswitch sensors are provided along with those for one example application for which riboswitch sensors are ideally suited.
    MeSH term(s) Bacteria/cytology ; Bacteria/metabolism ; Biosensing Techniques/methods ; Culture Media/chemistry ; Culture Media/metabolism ; Genes, Reporter/genetics ; Molecular Biology/methods ; Nucleic Acid Conformation ; Riboswitch/genetics
    Chemical Substances Culture Media ; Riboswitch
    Language English
    Publishing date 2014
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-62703-730-3_14
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Alternate subunit assembly diversifies the function of a bacterial toxin.

    Fowler, Casey C / Stack, Gabrielle / Jiao, Xuyao / Lara-Tejero, Maria / Galán, Jorge E

    Nature communications

    2019  Volume 10, Issue 1, Page(s) 3684

    Abstract: Bacterial toxins with an ... ...

    Abstract Bacterial toxins with an AB
    MeSH term(s) Animals ; Bacterial Toxins/genetics ; Cell Line, Tumor ; Humans ; Mice ; Protein Multimerization/genetics ; Protein Subunits/genetics ; Salmonella typhi/genetics ; Sequence Homology, Nucleic Acid ; Virulence Factors/genetics
    Chemical Substances Bacterial Toxins ; Protein Subunits ; Virulence Factors
    Language English
    Publishing date 2019-08-15
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-019-11592-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Molecular Insights into the Assembly and Functional Diversification of Typhoid Toxin.

    Liu, Xiaoyu / Chen, Zhe / Jiao, Xuyao / Jiang, Xukai / Qiu, Jicheng / You, Fuping / Long, Hongan / Cao, Hongzhi / Fowler, Casey C / Gao, Xiang

    mBio

    2022  Volume 13, Issue 1, Page(s) e0191621

    Abstract: Typhoid toxin is an ... ...

    Abstract Typhoid toxin is an A
    MeSH term(s) Humans ; Typhoid Fever/microbiology ; Bacterial Toxins/metabolism ; Salmonella typhi ; Virulence Factors/metabolism ; Polysaccharides/metabolism
    Chemical Substances Bacterial Toxins ; Virulence Factors ; Polysaccharides
    Language English
    Publishing date 2022-01-11
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2557172-2
    ISSN 2150-7511 ; 2161-2129
    ISSN (online) 2150-7511
    ISSN 2161-2129
    DOI 10.1128/mbio.01916-21
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  9. Article ; Online: Artificial riboswitch selection: A FACS-based approach.

    Ghazi, Zohaib / Fowler, Casey C / Li, Yingfu

    Methods in molecular biology (Clifton, N.J.)

    2014  Volume 1111, Page(s) 57–75

    Abstract: Riboswitches have a number of characteristics that make them ideal regulatory elements for a wide range of synthetic biology applications. To maximize their utility, methods are required to create custom riboswitches de novo or to modify existing ... ...

    Abstract Riboswitches have a number of characteristics that make them ideal regulatory elements for a wide range of synthetic biology applications. To maximize their utility, methods are required to create custom riboswitches de novo or to modify existing riboswitches to suit specific experimental needs. This chapter describes such a method, which exploits fluorescence-activated cell sorting (FACS) to quickly and efficiently sort through large libraries of riboswitch-like sequences to identify those with the desired activity. Suggestions for the experimental setup are provided, along with detailed protocols for testing and optimizing FACS conditions FACS selection steps, and follow-up assays to identify and characterize individual riboswitches.
    MeSH term(s) Aptamers, Nucleotide/chemistry ; Base Sequence ; Flow Cytometry/methods ; Riboswitch
    Chemical Substances Aptamers, Nucleotide ; Riboswitch
    Language English
    Publishing date 2014
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-62703-755-6_5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Emerging insights into the biology of typhoid toxin.

    Fowler, Casey C / Chang, Shu-Jung / Gao, Xiang / Geiger, Tobias / Stack, Gabrielle / Galán, Jorge E

    Current opinion in microbiology

    2017  Volume 35, Page(s) 70–77

    Abstract: Typhoid toxin is a unique ... ...

    Abstract Typhoid toxin is a unique A
    MeSH term(s) Animals ; Bacterial Toxins/metabolism ; Exotoxins/metabolism ; Humans ; Mice ; Salmonella typhi/metabolism ; Salmonella typhi/pathogenicity ; Typhoid Fever/microbiology ; Typhoid Fever/physiopathology ; Typhoid Fever/prevention & control ; Typhoid Fever/therapy ; Virulence Factors/metabolism
    Chemical Substances Bacterial Toxins ; Exotoxins ; Virulence Factors
    Language English
    Publishing date 2017-02-16
    Publishing country England
    Document type Journal Article ; Review ; Research Support, N.I.H., Extramural
    ZDB-ID 1418474-6
    ISSN 1879-0364 ; 1369-5274
    ISSN (online) 1879-0364
    ISSN 1369-5274
    DOI 10.1016/j.mib.2017.01.012
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    In stock of ZB MED Cologne/Königswinter

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