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  1. AU="Fraser, Alice J"
  2. AU="Arenas, Sara"
  3. AU=Godehardt Antonia W
  4. AU="Serna, Brigitte"
  5. AU="Kearney, Kathleen E"
  6. AU=HAN SHUWEN
  7. AU="Abdul Basir"
  8. AU="Linden, David R"
  9. AU="Schaefer, Jan"
  10. AU="Sprent, Noah"
  11. AU="Dreyer, Benard P"
  12. AU=Borchman Douglas
  13. AU="Navarro-Garcia, Fernando"
  14. AU=Vandenbon Alexis
  15. AU="Alonso-Ventura, Vanesa"
  16. AU="Ganhewa, Aparna D"
  17. AU="Draggoo, V"
  18. AU="Natalia Skogberg"
  19. AU="Hiroaki Itoh" AU="Hiroaki Itoh"
  20. AU="Nicolette de Keizer"
  21. AU="Jamjoom, Dima"
  22. AU="Seeman, Tomas"
  23. AU="Popescu, S"
  24. AU="Kurtul, Irmak"
  25. AU="Christofferson, Scott"
  26. AU="Balghith, Mohammed A"
  27. AU="Banu, Qamar"
  28. AU="Giangregorio, Lora"
  29. AU="Stafiej, Patrycja"
  30. AU="Lau, Vincent W-H"
  31. AU="Francesca Storici"
  32. AU="Coulter-Mackie, Marion"
  33. AU="Mayank Goyal"
  34. AU="Lempke, Olga M"
  35. AU="Khan, Asad Majeed"
  36. AU=Ismail Mohd Iswadi
  37. AU="Jewel Park"
  38. AU="Hunter-Smith, David J"
  39. AU="Requião-Moura, Lúcio Roberto"
  40. AU=DesRochers Teresa M.
  41. AU="Kruschwitz, Sabine"
  42. AU=Sriwijiatalai Won
  43. AU="Bozzaro, Claudia"
  44. AU="Beckendorf, C"
  45. AU="Birge, N W"
  46. AU="Hoang, Oi Pui"
  47. AU="Saradha Baskaran"
  48. AU="Culotta, Lorenza"
  49. AU=Cleaver Ondine
  50. AU="Jordan A. Kreidberg"
  51. AU="Al-Marshoud, Majida"
  52. AU="David S Hui"
  53. AU="Manjappa, Shivaprasad"
  54. AU="Balkan, S"
  55. AU="Chen, Emma"
  56. AU="Delean, Ada"
  57. AU="Gurao, Ankita"
  58. AU="Lang, Zhen"
  59. AU="Mahnaz Mohammadpour"
  60. AU="Britta Grillitsch"
  61. AU=Hoeffner Ellen G
  62. AU="Al Harbi, Shmeylan"
  63. AU=Polevoda Bogdan
  64. AU="Raffaele Galiero"
  65. AU=Hruskova Z
  66. AU="Ayers, J"
  67. AU="Cohen, A D"
  68. AU="Brunetti, Gian Luca"
  69. AU=Andrade Daniel
  70. AU=Hay William W Jr

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  1. Artikel ; Online: A high-resolution melt curve toolkit to identify lineage-defining SARS-CoV-2 mutations.

    Fraser, Alice J / Greenland-Bews, Caitlin / Kelly, Daniel / Williams, Christopher T / Body, Richard / Adams, Emily R / Atienzar, Ana Cubas / Edwards, Thomas / Allen, David J

    Scientific reports

    2023  Band 13, Heft 1, Seite(n) 3887

    Abstract: The emergence of severe acute respiratory syndrome 2 (SARS-CoV-2) variants of concern (VOCs), with mutations linked to increased transmissibility, vaccine escape and virulence, has necessitated the widespread genomic surveillance of SARS-CoV-2. This has ... ...

    Abstract The emergence of severe acute respiratory syndrome 2 (SARS-CoV-2) variants of concern (VOCs), with mutations linked to increased transmissibility, vaccine escape and virulence, has necessitated the widespread genomic surveillance of SARS-CoV-2. This has placed a strain on global sequencing capacity, especially in areas lacking the resources for large scale sequencing activities. Here we have developed three separate multiplex high-resolution melting assays to enable the identification of Alpha, Beta, Delta and Omicron VOCs. The assays were evaluated against whole genome sequencing on upper-respiratory swab samples collected during the Alpha, Delta and Omicron [BA.1] waves of the UK pandemic. The sensitivities of the eight individual primer sets were all 100%, and specificity ranged from 94.6 to 100%. The multiplex HRM assays have potential as a tool for high throughput surveillance of SARS-CoV-2 VOCs, particularly in areas with limited genomics facilities.
    Mesh-Begriff(e) Humans ; COVID-19 ; SARS-CoV-2/genetics ; Mutation ; Biological Assay ; Genomics
    Sprache Englisch
    Erscheinungsdatum 2023-03-08
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-30754-1
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Piperacillin/tazobactam-resistant, cephalosporin-susceptible

    Edwards, Thomas / Heinz, Eva / van Aartsen, Jon / Howard, Alex / Roberts, Paul / Corless, Caroline / Fraser, Alice J / Williams, Christopher T / Bulgasim, Issra / Cuevas, Luis E / Parry, Christopher M / Roberts, Adam P / Adams, Emily R / Mason, Jenifer / Hubbard, Alasdair T M

    Microbial genomics

    2022  Band 8, Heft 4

    Abstract: Resistance to piperacillin/tazobactam (TZP) ... ...

    Abstract Resistance to piperacillin/tazobactam (TZP) in
    Mesh-Begriff(e) Anti-Bacterial Agents/pharmacology ; Cephalosporins/pharmacology ; Escherichia coli/genetics ; Escherichia coli Infections/epidemiology ; Humans ; Piperacillin, Tazobactam Drug Combination ; Sepsis
    Chemische Substanzen Anti-Bacterial Agents ; Cephalosporins ; Piperacillin, Tazobactam Drug Combination (157044-21-8)
    Sprache Englisch
    Erscheinungsdatum 2022-04-11
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2835258-0
    ISSN 2057-5858 ; 2057-5858
    ISSN (online) 2057-5858
    ISSN 2057-5858
    DOI 10.1099/mgen.0.000789
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Piperacillin/tazobactam resistance in a clinical isolate of Escherichia coli due to IS26-mediated amplification of bla

    Hubbard, Alasdair T M / Mason, Jenifer / Roberts, Paul / Parry, Christopher M / Corless, Caroline / van Aartsen, Jon / Howard, Alex / Bulgasim, Issra / Fraser, Alice J / Adams, Emily R / Roberts, Adam P / Edwards, Thomas

    Nature communications

    2020  Band 11, Heft 1, Seite(n) 4915

    Abstract: A phenotype of Escherichia coli and Klebsiella pneumoniae, resistant to piperacillin/tazobactam (TZP) but susceptible to carbapenems and 3rd generation cephalosporins, has emerged. The resistance mechanism associated with this phenotype has been ... ...

    Abstract A phenotype of Escherichia coli and Klebsiella pneumoniae, resistant to piperacillin/tazobactam (TZP) but susceptible to carbapenems and 3rd generation cephalosporins, has emerged. The resistance mechanism associated with this phenotype has been identified as hyperproduction of the β-lactamase TEM. However, the mechanism of hyperproduction due to gene amplification is not well understood. Here, we report a mechanism of gene amplification due to a translocatable unit (TU) excising from an IS26-flanked pseudo-compound transposon, PTn6762, which harbours bla
    Mesh-Begriff(e) Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Chromosomes, Bacterial/genetics ; DNA Transposable Elements/genetics ; DNA, Bacterial/genetics ; Drug Resistance, Multiple, Bacterial/genetics ; Drug Therapy, Combination/methods ; Escherichia coli/drug effects ; Escherichia coli/genetics ; Escherichia coli/isolation & purification ; Escherichia coli Infections/drug therapy ; Escherichia coli Infections/microbiology ; Escherichia coli Proteins/genetics ; Gene Amplification ; Gene Expression Regulation, Bacterial ; Genome, Bacterial/genetics ; Humans ; Microbial Sensitivity Tests ; Piperacillin/pharmacology ; Piperacillin/therapeutic use ; Polymorphism, Restriction Fragment Length ; RNA, Ribosomal, 16S/genetics ; Tazobactam/pharmacology ; Tazobactam/therapeutic use ; Whole Genome Sequencing ; beta-Lactamases/genetics
    Chemische Substanzen Anti-Bacterial Agents ; DNA Transposable Elements ; DNA, Bacterial ; Escherichia coli Proteins ; RNA, Ribosomal, 16S ; beta-Lactamases (EC 3.5.2.6) ; beta-lactamase TEM-1 (EC 3.5.2.6) ; Tazobactam (SE10G96M8W) ; Piperacillin (X00B0D5O0E)
    Sprache Englisch
    Erscheinungsdatum 2020-10-01
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-020-18668-2
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel ; Online: Saliva Alternative to Upper Respiratory Swabs for SARS-CoV-2 Diagnosis.

    Byrne, Rachel L / Kay, Grant A / Kontogianni, Konstantina / Aljayyoussi, Ghaith / Brown, Lottie / Collins, Andrea M / Cuevas, Luis E / Ferreira, Daniela M / Fraser, Alice J / Garrod, Gala / Hill, Helen / Hughes, Grant L / Menzies, Stefanie / Mitsi, Elena / Owen, Sophie I / Patterson, Edward I / Williams, Christopher T / Hyder-Wright, Angela / Adams, Emily R /
    Cubas-Atienzar, Ana I

    Emerging infectious diseases

    2020  Band 26, Heft 11, Seite(n) 2770–2771

    Abstract: PCR of upper respiratory specimens is the diagnostic standard for severe acute respiratory syndrome coronavirus 2 infection. However, saliva sampling is an easy alternative to nasal and throat swabbing. We found similar viral loads in saliva samples and ... ...

    Abstract PCR of upper respiratory specimens is the diagnostic standard for severe acute respiratory syndrome coronavirus 2 infection. However, saliva sampling is an easy alternative to nasal and throat swabbing. We found similar viral loads in saliva samples and in nasal and throat swab samples from 110 patients with coronavirus disease.
    Mesh-Begriff(e) Adult ; Aged ; Betacoronavirus/isolation & purification ; COVID-19 ; COVID-19 Testing ; Clinical Laboratory Techniques/methods ; Coronavirus Infections/diagnosis ; Female ; Humans ; Male ; Middle Aged ; Nose/virology ; Pandemics ; Pharynx/virology ; Pneumonia, Viral/diagnosis ; SARS-CoV-2 ; Saliva/virology ; Viral Load
    Schlagwörter covid19
    Sprache Englisch
    Erscheinungsdatum 2020-09-11
    Erscheinungsland United States
    Dokumenttyp Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1380686-5
    ISSN 1080-6059 ; 1080-6040
    ISSN (online) 1080-6059
    ISSN 1080-6040
    DOI 10.3201/eid2611.203283
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel ; Online: Application of a High-Resolution Melt Assay for Monitoring SARS-CoV-2 Variants in Burkina Faso and Kenya

    Greenland-Bews, Caitlin / Shah, Sonal / Achieng, Morine / Badoum, Emilie S / Bah, Yaya / Barsosio, Hellen C / Brazal-Monzó, Helena / Canizales, Jennifer / Drabko, Anna / Fraser, Alice J / Hannan, Luke / Jarju, Sheikh J / Kaboré, Jean-Moise T / Kujabi, Mariama A / Lesosky, Maia / Manneh, Jarra / Marlais, Tegwen / Matthewman, Julian / Nebie, Issa /
    Onyango, Eric D / Ouedraogo, Alphonse / Otieno, Kephas / Serme, Samuel S / Sirima, Sodiomon B / Soulama, Ben I / Tangara, Brian / Tiono, Alfred B / Wu, William / Sesay, Abdul Karim / Soulama, Issiaka / Kariuki, Simon / Drakeley, Chris / ter Kuile, Feiko O / Adams, Emily R / Allen, David J / Edwards, Thomas

    medRxiv

    Abstract: The rapid emergence and global dissemination of SARS-CoV-2 highlighted a need for robust, adaptable surveillance systems. However, financial and infrastructure requirements for whole genome sequencing (WGS) mean most surveillance data have come from ... ...

    Abstract The rapid emergence and global dissemination of SARS-CoV-2 highlighted a need for robust, adaptable surveillance systems. However, financial and infrastructure requirements for whole genome sequencing (WGS) mean most surveillance data have come from higher-resource geographies, despite unprecedented investment in sequencing in low-middle income countries (LMICs) throughout the SARS-CoV-2 pandemic. Consequently, the molecular epidemiology of SARS-CoV-2 in some LMICs is limited, and there is a need for more cost-accessible technologies to help close data gaps for surveillance of SARS-CoV-2 variants. To address this, we have developed two high-resolution melt curve (HRM) assays that target key variant-defining mutations in the SARS-CoV-2 genome, which give unique signature profiles that define different SARS-CoV-2 variants of concern (VOCs). Extracted RNA from SARS-CoV-2 positive samples collected from 205 participants (112 in Burkina Faso, 93 in Kenya) on the day of enrolment in the MALCOV study (Malaria as a Risk Factor for COVID-19) between February 2021 and February 2022 were analysed using our optimised HRM assays and compared to Next Generation Sequencing (NGS) on Oxford Nanopore MinION . With NGS as a reference, two HRM assays, HRM-VOC-1 and HRM-VOC-2, demonstrated sensitivity/specificity of 100%/99.29% and 92.86/99.39%, respectively, for detecting Alpha, 90.08%/100% and 92.31%/100% for Delta and 93.75%/100% and 100%/99.38% for Omicron. The assays described here provide a lower-cost approach (<$1 per sample) to conducting molecular epidemiology, capable of high-throughput testing. We successfully scaled up the HRM-VOC-2 assay to screen a total of 506 samples from which we were able to show the replacement of Alpha with the introduction of Delta and the replacement of Delta by the Omicron variant in this community in Kisumu, Kenya. These assays are readily adaptable and can focus on local epidemiological surveillance questions or be updated quickly to accommodate the emergence of a novel variant or adapt to novel and emerging pathogens
    Schlagwörter covid19
    Sprache Englisch
    Erscheinungsdatum 2024-04-16
    Verlag Cold Spring Harbor Laboratory Press
    Dokumenttyp Artikel ; Online
    DOI 10.1101/2024.04.11.24305244
    Datenquelle COVID19

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  6. Artikel ; Online: Twelve lateral flow immunoassays (LFAs) to detect SARS-CoV-2 antibodies.

    Owen, Sophie I / Williams, Christopher T / Garrod, Gala / Fraser, Alice J / Menzies, Stefanie / Baldwin, Lisa / Brown, Lottie / Byrne, Rachel L / Collins, Andrea M / Cubas-Atienzar, Ana I / de Vos, Margaretha / Edwards, Thomas / Escadafal, Camille / Ferreira, Daniela M / Fletcher, Tom / Hyder-Wright, Angela / Kay, Grant A / Kontogianni, Konstantina / Mason, Jenifer /
    Mitsi, Elena / Planche, Tim / Sacks, Jilian A / Taylor, Joseph / Todd, Stacy / Tully, Caroline / Cuevas, Luis E / Adams, Emily R

    The Journal of infection

    2021  Band 84, Heft 3, Seite(n) 355–360

    Abstract: Background: There are an abundance of commercially available lateral flow assays (LFAs) that detect antibodies to SARS-CoV-2. Whilst these are usually evaluated by the manufacturer, externally performed diagnostic accuracy studies to assess performance ... ...

    Abstract Background: There are an abundance of commercially available lateral flow assays (LFAs) that detect antibodies to SARS-CoV-2. Whilst these are usually evaluated by the manufacturer, externally performed diagnostic accuracy studies to assess performance are essential. Herein we present an evaluation of 12 LFAs.
    Methods: Sera from 100 SARS-CoV-2 reverse-transcriptase polymerase chain reaction (RT-PCR) positive participants were recruited through the FASTER study. A total of 105 pre-pandemic sera from participants with other infections were included as negative samples.
    Results: At presentation sensitivity against RT-PCR ranged from 37.4 to 79% for IgM/IgG, 30.3-74% for IgG, and 21.2-67% for IgM. Sensitivity for IgM/IgG improved ≥ 21 days post symptom onset for 10/12 tests. Specificity ranged from 74.3 to 99.1% for IgM/IgG, 82.9-100% for IgG, and 75.2-98% for IgM. Compared to the EuroImmun IgG enzyme-linked immunosorbent assay (ELISA), sensitivity and specificity ranged from 44.6 to 95.4% and 85.4-100%, respectively.
    Conclusion: There are many LFAs available with varied sensitivity and specificity. Understanding the diagnostic accuracy of these tests will be vital as we come to rely more on the antibody status of a person moving forward, and as such manufacturer-independent evaluations are crucial.
    Mesh-Begriff(e) Antibodies, Viral ; COVID-19/diagnosis ; Humans ; Immunoassay ; Immunoglobulin G ; Immunoglobulin M ; SARS-CoV-2 ; Sensitivity and Specificity
    Chemische Substanzen Antibodies, Viral ; Immunoglobulin G ; Immunoglobulin M
    Sprache Englisch
    Erscheinungsdatum 2021-12-11
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 424417-5
    ISSN 1532-2742 ; 0163-4453
    ISSN (online) 1532-2742
    ISSN 0163-4453
    DOI 10.1016/j.jinf.2021.12.007
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  7. Artikel: Saliva Alternative to Upper Respiratory Swabs for SARS-CoV-2 Diagnosis

    Byrne, Rachel L / Kay, Grant A / Kontogianni, Konstantina / Aljayyoussi, Ghaith / Brown, Lottie / Collins, Andrea M / Cuevas, Luis E / Ferreira, Daniela M / Fraser, Alice J / Garrod, Gala / Hill, Helen / Hughes, Grant L / Menzies, Stefanie / Mitsi, Elena / Owen, Sophie I / Patterson, Edward I / Williams, Christopher T / Hyder-Wright, Angela / Adams, Emily R /
    Cubas-Atienzar, Ana I

    Emerg Infect Dis

    Abstract: PCR of upper respiratory specimens is the diagnostic standard for severe acute respiratory syndrome coronavirus 2 infection. However, saliva sampling is an easy alternative to nasal and throat swabbing. We found similar viral loads in saliva samples and ... ...

    Abstract PCR of upper respiratory specimens is the diagnostic standard for severe acute respiratory syndrome coronavirus 2 infection. However, saliva sampling is an easy alternative to nasal and throat swabbing. We found similar viral loads in saliva samples and in nasal and throat swab samples from 110 patients with coronavirus disease.
    Schlagwörter covid19
    Verlag WHO
    Dokumenttyp Artikel
    Anmerkung WHO #Covidence: #760833
    Datenquelle COVID19

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  8. Artikel ; Online: IgG Seroconversion and Pathophysiology in Severe Acute Respiratory Syndrome Coronavirus 2 Infection.

    Staines, Henry M / Kirwan, Daniela E / Clark, David J / Adams, Emily R / Augustin, Yolanda / Byrne, Rachel L / Cocozza, Michael / Cubas-Atienzar, Ana I / Cuevas, Luis E / Cusinato, Martina / Davies, Benedict M O / Davis, Mark / Davis, Paul / Duvoix, Annelyse / Eckersley, Nicholas M / Forton, Daniel / Fraser, Alice J / Garrod, Gala / Hadcocks, Linda /
    Hu, Qinxue / Johnson, Michael / Kay, Grant A / Klekotko, Kesja / Lewis, Zawditu / Macallan, Derek C / Mensah-Kane, Josephine / Menzies, Stefanie / Monahan, Irene / Moore, Catherine M / Nebe-von-Caron, Gerhard / Owen, Sophie I / Sainter, Chris / Sall, Amadou A / Schouten, James / Williams, Christopher T / Wilkins, John / Woolston, Kevin / Fitchett, Joseph R A / Krishna, Sanjeev / Planche, Tim

    Emerging infectious diseases

    2020  Band 27, Heft 1

    Abstract: We investigated the dynamics of seroconversion in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. During March 29-May 22, 2020, we collected serum samples and associated clinical data from 177 persons in London, UK, who had SARS- ... ...

    Abstract We investigated the dynamics of seroconversion in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. During March 29-May 22, 2020, we collected serum samples and associated clinical data from 177 persons in London, UK, who had SARS-CoV-2 infection. We measured IgG against SARS-CoV-2 and compared antibody levels with patient outcomes, demographic information, and laboratory characteristics. We found that 2.0%-8.5% of persons did not seroconvert 3-6 weeks after infection. Persons who seroconverted were older, were more likely to have concurrent conditions, and had higher levels of inflammatory markers. Non-White persons had higher antibody concentrations than those who identified as White; these concentrations did not decline during follow-up. Serologic assay results correlated with disease outcome, race, and other risk factors for severe SARS-CoV-2 infection. Serologic assays can be used in surveillance to clarify the duration and protective nature of humoral responses to SARS-CoV-2 infection.
    Mesh-Begriff(e) Adult ; Aged ; Antibodies, Viral/blood ; COVID-19/blood ; COVID-19/immunology ; COVID-19/physiopathology ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Immunoglobulin G/blood ; Male ; Middle Aged ; Reverse Transcriptase Polymerase Chain Reaction ; SARS-CoV-2 ; Seroconversion
    Chemische Substanzen Antibodies, Viral ; Immunoglobulin G
    Sprache Englisch
    Erscheinungsdatum 2020-11-30
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 1380686-5
    ISSN 1080-6059 ; 1080-6040
    ISSN (online) 1080-6059
    ISSN 1080-6040
    DOI 10.3201/eid2701.203074
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 4778: Hefte anzeigen Standort:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
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  9. Artikel ; Online: Rapid development of COVID-19 rapid diagnostics for low resource settings: accelerating delivery through transparency, responsiveness, and open collaboration

    Adams, Emily R / Augustin, Yolanda / Byrne, Rachel L / Clark, David J / Cocozza, Michael / Cubas-Atienzar, Ana I / Cuevas, Luis E / Cusinato, Martina / Davies, Benedict M. O. / Davies, Mark / Davies, Paul / Duvoix, Annelyse / Eckersley, Nicholas M / Edwards, Thomas / Fletcher, Thomas / Fraser, Alice j / Garrod, Gala / Hadcocks, Linda / Hu, QInxue /
    johnson, Michael / Kay, Grant A / Keymer, Katherin / Kirwan, Daniela / Klekotko, Kesja / Lewis, Zawditu / Mason, Jenifer / Mensah-Kane, Josie / Menzies, Stefanie / Monahan, Irene / Moore, Catherine M / Nebe-von-Caron, Gerhard / Owen, Sophie I / Planche, Tim / Sainter, Chris / Schouten, James / Staines, Henry M / Turtle, Lance / Williams, Chris / Wilkins, John / Woolston, Kevin / Sall, Amadou A / Fitchett, Joseph R.A. / Krishna, Sanjeev

    Abstract: In January, Mologic, embarked on a product development pathway for COVID-19 diagnostics focusing on ELISA and rapid diagnostic tests (RDTs), with anticipated funding from Wellcome Trust and DFID. 755 clinical samples from known COVID-19 patients and ... ...

    Abstract In January, Mologic, embarked on a product development pathway for COVID-19 diagnostics focusing on ELISA and rapid diagnostic tests (RDTs), with anticipated funding from Wellcome Trust and DFID. 755 clinical samples from known COVID-19 patients and hospital negative controls were tested on Mologics IgG ELISA. The reported sensitivity on 191 SGUL prospectively enrolled patients was 95% on day 7 or more post diagnosis, and 97% 10 days or more post-diagnosis. A specificity panel comprising 564 samples pre-December 2019 were tested to include most common respiratory pathogens, other types of coronavirus, and flaviviruses. Specificity in this panel was 97%. This is the first in a series of Mologic products for COVID-19, which will be deployed for COVID-19 diagnosis, contact tracing and sero-epidemiological studies to estimate disease burden and transmission with a focus on ensuring access, affordability, and availability to lowest resource settings.
    Schlagwörter covid19
    Verlag MedRxiv; WHO
    Dokumenttyp Artikel ; Online
    Anmerkung WHO #Covidence: #20082099
    DOI 10.1101/2020.04.29.20082099
    Datenquelle COVID19

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  10. Artikel ; Online: Rapid development of COVID-19 rapid diagnostics for low resource settings: accelerating delivery through transparency, responsiveness, and open collaboration

    Adams, Emily R / Augustin, Yolanda / Byrne, Rachel L / Clark, David J / Cocozza, Michael / Cubas-Atienzar, Ana I / Cuevas, Luis E / Cusinato, Martina / Davies, Benedict M. O. / Davies, Mark / Davies, Paul / Duvoix, Annelyse / Eckersley, Nicholas M / Edwards, Thomas / Fletcher, Thomas / Fraser, Alice j / Garrod, Gala / Hadcocks, Linda / Hu, QInxue /
    johnson, Michael / Kay, Grant A / Keymer, Katherin / Kirwan, Daniela / Klekotko, Kesja / Lewis, Zawditu / Mason, Jenifer / Mensah-Kane, Josie / Menzies, Stefanie / Monahan, Irene / Moore, Catherine M / Nebe-von-Caron, Gerhard / Owen, Sophie I / Planche, Tim / Sainter, Chris / Schouten, James / Staines, Henry M / Turtle, Lance / Williams, Chris / Wilkins, John / Woolston, Kevin / Sall, Amadou A / Fitchett, Joseph R.A. / Krishna, Sanjeev

    medRxiv

    Abstract: In January, Mologic, embarked on a product development pathway for COVID-19 diagnostics focusing on ELISA and rapid diagnostic tests (RDTs), with anticipated funding from Wellcome Trust and DFID. 755 clinical samples from known COVID-19 patients and ... ...

    Abstract In January, Mologic, embarked on a product development pathway for COVID-19 diagnostics focusing on ELISA and rapid diagnostic tests (RDTs), with anticipated funding from Wellcome Trust and DFID. 755 clinical samples from known COVID-19 patients and hospital negative controls were tested on Mologics IgG ELISA. The reported sensitivity on 191 SGUL prospectively enrolled patients was 95% on day 7 or more post diagnosis, and 97% 10 days or more post-diagnosis. A specificity panel comprising 564 samples pre-December 2019 were tested to include most common respiratory pathogens, other types of coronavirus, and flaviviruses. Specificity in this panel was 97%. This is the first in a series of Mologic products for COVID-19, which will be deployed for COVID-19 diagnosis, contact tracing and sero-epidemiological studies to estimate disease burden and transmission with a focus on ensuring access, affordability, and availability to lowest resource settings.
    Schlagwörter covid19
    Sprache Englisch
    Erscheinungsdatum 2020-05-05
    Verlag Cold Spring Harbor Laboratory Press
    Dokumenttyp Artikel ; Online
    DOI 10.1101/2020.04.29.20082099
    Datenquelle COVID19

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