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Article ; Online: Do Ionic Liquids Exhibit the Required Characteristics to Dissolve, Extract, Stabilize, and Purify Proteins? Past-Present-Future Assessment.

Bharmoria, Pankaj / Tietze, Alesia A / Mondal, Dibyendu / Kang, Tejwant Singh / Kumar, Arvind / Freire, Mara G

2024 Volume 124, Issue 6, Page(s) 3037–3084

Abstract: Proteins are highly labile molecules, thus requiring the presence of appropriate solvents and excipients in their liquid milieu to keep their stability and biological activity. In this field, ionic liquids (ILs) have gained momentum in the past years, ... ...

Abstract	Proteins are highly labile molecules, thus requiring the presence of appropriate solvents and excipients in their liquid milieu to keep their stability and biological activity. In this field, ionic liquids (ILs) have gained momentum in the past years, with a relevant number of works reporting their successful use to dissolve, stabilize, extract, and purify proteins. Different approaches in protein-IL systems have been reported, namely, proteins dissolved in (
MeSH term(s)	Ionic Liquids/chemistry ; Proteins/chemistry ; Solvents/chemistry ; Water/chemistry ; Polymers
Chemical Substances	Ionic Liquids ; Proteins ; Solvents ; Water (059QF0KO0R) ; Polymers
Language	English
Publishing date	2024-03-04
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	207949-5
ISSN	1520-6890 ; 0009-2665
ISSN (online)	1520-6890
ISSN	0009-2665
DOI	10.1021/acs.chemrev.3c00551
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Article: Corrigendum: Ionic-liquid-based approaches to improve biopharmaceuticals downstream processing and formulation.

Almeida, Catarina / Pedro, Augusto Q / Tavares, Ana P M / Neves, Márcia C / Freire, Mara G

Frontiers in bioengineering and biotechnology

2023 Volume 11, Page(s) 1213921

Abstract: This corrects the article DOI: 10.3389/fbioe.2023.1037436.]. ...

Abstract	[This corrects the article DOI: 10.3389/fbioe.2023.1037436.].
Language	English
Publishing date	2023-05-09
Publishing country	Switzerland
Document type	Published Erratum
ZDB-ID	2719493-0
ISSN	2296-4185
ISSN	2296-4185
DOI	10.3389/fbioe.2023.1213921
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Article ; Online: Towards the Use of Adsorption Methods for the Removal of Purines from Beer.

Almeida, Catarina / Neves, Márcia C / Freire, Mara G

Molecules (Basel, Switzerland)

2021 Volume 26, Issue 21

Abstract: Beer corresponds to a fermented alcoholic beverage composed of several components, including purine compounds. These molecules, when ingested by humans, can be catabolized into uric acid, contributing to uric acid's level increase in serum, which may ... ...

Abstract	Beer corresponds to a fermented alcoholic beverage composed of several components, including purine compounds. These molecules, when ingested by humans, can be catabolized into uric acid, contributing to uric acid's level increase in serum, which may lead to hyperuricemia and gout. To assure a proper management of this disease, physicians recommend restrictive dietary measures, particularly by avoiding the consumption of beer. Therefore, it is of relevance to develop efficient methods to remove purine compounds from alcoholic beverages such as beer. In this review, we provide an introduction on fermented alcoholic beverages, with emphasis on beer, as well as its purine compounds and their role in uric acid metabolism in the human body in relation to hyperuricemia and gout development. The several reported enzymatic, biological and adsorption methods envisaging purine compounds' removal are then reviewed. Some enzymatic and biological methods present drawbacks, which can be overcome by adsorption methods. Within adsorption methods, adsorbent materials, such as activated carbon or charcoal, have been reported and applied to beer or wort samples, showing an excellent capacity for adsorbing and removing purine compounds. Although the main topic of this review is on the removal of purine compounds from beer, other studies involving other matrices rather than beer or wort that are rich in purines are included, since they provide relevant clues on designing efficient removal processes. By ensuring the selective removal of purine compounds from this beverage, beer can be taken by hyperuricemic and gouty patients, avoiding restrictive dietary measures, while decreasing the related healthcare economic burden.
MeSH term(s)	Adsorption ; Beer/analysis ; Molecular Structure ; Particle Size ; Purines/chemistry ; Purines/isolation & purification ; Surface Properties
Chemical Substances	Purines
Language	English
Publishing date	2021-10-26
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules26216460
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Article: Ionic-liquid-based approaches to improve biopharmaceuticals downstream processing and formulation.

Almeida, Catarina / Pedro, Augusto Q / Tavares, Ana P M / Neves, Márcia C / Freire, Mara G

Frontiers in bioengineering and biotechnology

2023 Volume 11, Page(s) 1037436

Abstract: The emergence of biopharmaceuticals, including proteins, nucleic acids, peptides, and vaccines, revolutionized the medical field, contributing to significant advances in the prophylaxis and treatment of chronic and life-threatening diseases. However, ... ...

Abstract	The emergence of biopharmaceuticals, including proteins, nucleic acids, peptides, and vaccines, revolutionized the medical field, contributing to significant advances in the prophylaxis and treatment of chronic and life-threatening diseases. However, biopharmaceuticals manufacturing involves a set of complex upstream and downstream processes, which considerably impact their cost. In particular, despite the efforts made in the last decades to improve the existing technologies, downstream processing still accounts for more than 80% of the total biopharmaceutical production cost. On the other hand, the formulation of biological products must ensure they maintain their therapeutic performance and long-term stability, while preserving their physical and chemical structure. Ionic-liquid (IL)-based approaches arose as a promise alternative, showing the potential to be used in downstream processing to provide increased purity and recovery yield, as well as excipients for the development of stable biopharmaceutical formulations. This manuscript reviews the most important progress achieved in both fields. The work developed is critically discussed and complemented with a SWOT analysis.
Language	English
Publishing date	2023-02-07
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2719493-0
ISSN	2296-4185
ISSN	2296-4185
DOI	10.3389/fbioe.2023.1037436
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: 25th Anniversary of Molecules-Recent Advances in Green Chemistry.

de Gonzalo, Gonzalo / Freire, Mara G

Molecules (Basel, Switzerland)

2021 Volume 26, Issue 12

Abstract: Green Chemistry has been defined by the EPA as the design of chemical products and processes that reduce or eliminate the use or generation of hazardous substances [ ... ]. ...

Abstract	Green Chemistry has been defined by the EPA as the design of chemical products and processes that reduce or eliminate the use or generation of hazardous substances [...].
Language	English
Publishing date	2021-06-21
Publishing country	Switzerland
Document type	Editorial
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules26123768
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Article ; Online: Wood inspired biobased nanocomposite films composed of xylans, lignosulfonates and cellulose nanofibers for active food packaging.

Silva, José M / Vilela, Carla / Girão, Ana Violeta / Branco, Pedro C / Martins, João / Freire, Mara G / Silvestre, Armando J D / Freire, Carmen S R

Carbohydrate polymers

2024 Volume 337, Page(s) 122112

Abstract: The growing concerns on environmental pollution and sustainability have raised the interest on the development of functional biobased materials for different applications, including food packaging, as an alternative to the fossil resources-based ... ...

Abstract	The growing concerns on environmental pollution and sustainability have raised the interest on the development of functional biobased materials for different applications, including food packaging, as an alternative to the fossil resources-based counterparts, currently available in the market. In this work, functional wood inspired biopolymeric nanocomposite films were prepared by solvent casting of suspensions containing commercial beechwood xylans, cellulose nanofibers (CNF) and lignosulfonates (magnesium or sodium), in a proportion of 2:5:3 wt%, respectively. All films presented good homogeneity, translucency, and thermal stability up to 153 °C. The incorporation of CNF into the xylan/lignosulfonates matrix provided good mechanical properties to the films (Young's modulus between 1.08 and 3.79 GPa and tensile strength between 12.75 and 14.02 MPa). The presence of lignosulfonates imparted the films with antioxidant capacity (DPPH radical scavenging activity from 71.6 to 82.4 %) and UV barrier properties (transmittance ≤19.1 % (200-400 nm)). Moreover, the films obtained are able to successfully delay the browning of packaged fruit stored over 7 days at 4 °C. Overall, the obtained results show the potential of using low-cost and eco-friendly resources for the development of sustainable active food packaging materials.
MeSH term(s)	Food Packaging/methods ; Lignin/chemistry ; Lignin/analogs & derivatives ; Nanocomposites/chemistry ; Cellulose/chemistry ; Cellulose/analogs & derivatives ; Wood/chemistry ; Nanofibers/chemistry ; Xylans/chemistry ; Tensile Strength ; Antioxidants/chemistry ; Fruit/chemistry
Chemical Substances	Lignin (9005-53-2) ; Cellulose (9004-34-6) ; lignosulfuric acid (8062-15-5) ; Xylans ; Antioxidants
Language	English
Publishing date	2024-04-01
Publishing country	England
Document type	Journal Article
ZDB-ID	1501516-6
ISSN	1879-1344 ; 0144-8617
ISSN (online)	1879-1344
ISSN	0144-8617
DOI	10.1016/j.carbpol.2024.122112
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Article: Cholinium-Based Ionic Liquids as Efficient Media for Improving the Structural and Thermal Stability of Immunoglobulin G Antibodies

Dhiman, Diksha / Bisht, Meena / Tavares, Ana P. M. / Freire, Mara G. / Venkatesu, Pannuru

ACS sustainable chemistry & engineering. 2022 Apr. 19, v. 10, no. 17

2022

Abstract: Among antibody-based biopharmaceuticals, immunoglobulin G (IgG) plays a central role. However, IgG is a protein and as such easily loses stability, thus compromising its use as an effective therapeutic. To overcome this drawback, in this work, we ... ...

Abstract	Among antibody-based biopharmaceuticals, immunoglobulin G (IgG) plays a central role. However, IgG is a protein and as such easily loses stability, thus compromising its use as an effective therapeutic. To overcome this drawback, in this work, we evaluated cholinium-based ionic liquids (ILs) as effective stabilizers of IgG. We have investigated four ILs in aqueous solution, namely, cholinium acetate ([Ch][Ac]), cholinium chloride ([Ch]Cl), cholinium dihydrogen citrate ([Ch][Dhc]), and cholinium dihydrogen phosphate ([Ch][Dhp]). We used a quantitative approach using different spectroscopic and chromatographic techniques to evaluate the thermal and structural stability of IgG. Thermal fluorescence spectroscopy studies were performed to obtain the transition temperature (Tₘ) and the change in Gibbs free energy (ΔG) of IgG in all cholinium-based IL aqueous solutions. The results indicate an appreciable increase in Tₘ in the presence of [Ch][Ac] and [Ch]Cl. The thermodynamic parameters obtained from thermal fluorescence are compared with the structural stability of IgG by UV, fluorescence, CD, and FT-IR spectroscopies, as well as with size exclusion high-performance liquid chromatography and sodium dodecyl-sulfate polyacrylamide gel electrophoresis, showing that all results are in good agreement. Furthermore, the hydrodynamic diameter (dH) of the IgG as a function of the concentration of IL was analyzed using dynamic light scattering, showing favorable interactions between protein residues. Finally, molecular docking studies for IgG in ILs using Molegro virtual docker have been performed, reinforcing the main interactions ruling the IgG stability. Time dependent studies were also performed for one month to study the long-term stability of IgG at room temperature in presence of ILs. This work highlights the potential validity of using cholinium-based ILs in IgG formulations for enhancing its thermal and structural stability and thus the preservation of IgG antibodies.
Keywords	Gibbs free energy ; acetates ; ambient temperature ; aqueous solutions ; biopharmaceuticals ; citrates ; fluorescence ; fluorescence emission spectroscopy ; green chemistry ; high performance liquid chromatography ; hydrodynamics ; immunoglobulin G ; phosphates ; polyacrylamide gel electrophoresis ; quantitative analysis ; sodium ; therapeutics ; thermal stability
Language	English
Dates of publication	2022-0419
Size	p. 5404-5420.
Publishing place	American Chemical Society
Document type	Article
ISSN	2168-0485
DOI	10.1021/acssuschemeng.1c07979
Database	NAL-Catalogue (AGRICOLA)

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Article: Insights on the DNA Stability in Aqueous Solutions of Ionic Liquids.

Dinis, Teresa B V / Sousa, Fani / Freire, Mara G

Frontiers in bioengineering and biotechnology

2020 Volume 8, Page(s) 547857

Abstract: Deoxyribonucleic acid (DNA) carries the genetic information essential for the growth and functioning of living organisms, playing a significant role in life sciences research. However, the long-term storage and preservation of DNA, while ensuring its ... ...

Abstract	Deoxyribonucleic acid (DNA) carries the genetic information essential for the growth and functioning of living organisms, playing a significant role in life sciences research. However, the long-term storage and preservation of DNA, while ensuring its bioactivity, are still current challenges to overcome. In this work, aqueous solutions of ionic liquids (ILs) were investigated as potential preservation media for double stranded (dsDNA). A screening of several ILs, by combining the cholinium, tetrabutylammonium, tetrabutylphosphonium, and 1-ethyl-3-methylimidazolium, cations with the anions bromide, chloride, dihydrogen phosphate, acetate, and glycolate, was carried out in order to gather fundamental knowledge on the molecular features of ILs that improve the dsDNA stability. Different IL concentrations and the pH effect were also addressed. Circular dichroism (CD) spectroscopy was used to evaluate the conformational structure and stability of dsDNA. IL-DNA interactions were appraised by UV-Vis absorption spectrophotometry and
Language	English
Publishing date	2020-10-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2719493-0
ISSN	2296-4185
ISSN	2296-4185
DOI	10.3389/fbioe.2020.547857
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Article ; Online: Supported Ionic Liquids Used as Chromatographic Matrices in Bioseparation-An Overview.

Bernardo, Sandra C / Carapito, Rita / Neves, Márcia C / Freire, Mara G / Sousa, Fani

Molecules (Basel, Switzerland)

2022 Volume 27, Issue 5

Abstract: Liquid chromatography plays a central role in biomanufacturing, and, apart from its use as a preparative purification strategy, either in biopharmaceuticals or in fine chemicals industries, it is also very useful as an analytical tool for monitoring, ... ...

Abstract	Liquid chromatography plays a central role in biomanufacturing, and, apart from its use as a preparative purification strategy, either in biopharmaceuticals or in fine chemicals industries, it is also very useful as an analytical tool for monitoring, assessing, and characterizing diverse samples. The present review gives an overview of the progress of the chromatographic supports that have been used in the purification of high-value products (e.g., small molecules, organic compounds, proteins, and nucleic acids). Despite the diversity of currently available chromatographic matrices, the interest in innovative biomolecules emphasizes the need for novel, robust, and more efficient supports and ligands with improved selectivity. Accordingly, ionic liquids (ILs) have been investigated as novel ligands in chromatographic matrices. Given herein is an extensive review regarding the different immobilization strategies of ILs in several types of supports, namely in silica, Sepharose, and polymers. In addition to depicting their synthesis, the main application examples of these supports are also presented. The multiple interactions promoted by ILs are critically discussed concerning the improved selectivity towards target molecules. Overall, the versatility of supported ILs is here considered a critical point to their exploitation as alternatives to the more conventional liquid chromatographic matrices used in bioseparation processes.
MeSH term(s)	Chromatography, Liquid/methods ; Ionic Liquids/chemistry ; Polymers/chemistry ; Proteins
Chemical Substances	Ionic Liquids ; Polymers ; Proteins
Language	English
Publishing date	2022-02-28
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules27051618
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Article: New liquid supports in the development of integrated platforms for the reuse of oxidative enzymes and polydopamine production.

Magalhães, Flávia F / Pereira, Ana F / Freire, Mara G / Tavares, Ana P M

Frontiers in bioengineering and biotechnology

2022 Volume 10, Page(s) 1037322

Abstract: Polydopamine (PDA), a bioinspired polymer from mussel adhesive proteins, has attracted impressive attention as a novel coating for (nano) materials with an adequate conformal layer and adjustable thickness. Currently, PDA is obtained from dopamine ... ...

Abstract	Polydopamine (PDA), a bioinspired polymer from mussel adhesive proteins, has attracted impressive attention as a novel coating for (nano) materials with an adequate conformal layer and adjustable thickness. Currently, PDA is obtained from dopamine chemical oxidation under alkaline conditions, limiting its use in materials sensible to alkaline environments. Envisaging a widespread use of PDA, the polymerization of dopamine by enzymatic catalysis allows the dopamine polymerization in a large range of pHs, overcoming thus the limitations of conventional chemical oxidation. Moreover, the conventional method of polymerization is a time-consuming process and produces PDA films with poor stability, which restricts its applications. On the other hand, the main bottleneck of enzyme-based biocatalytic processes is the high cost of the single use of the enzyme. In this work, laccase was used to catalyse dopamine polymerization. To improve its performance, a liquid support for integrating the laccase and its reuse together with the PDA production and recovery was developed using aqueous biphasic systems (ABS). Firstly, dopamine polymerization by laccase was optimized in terms of pH, temperature and initial dopamine concentration. It was demonstrated that the highest enzymatic polymerization of dopamine was achieved at pH 5.5, 30°C and 2 mg ml
Language	English
Publishing date	2022-11-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2719493-0
ISSN	2296-4185
ISSN	2296-4185
DOI	10.3389/fbioe.2022.1037322
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