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  1. Article: Augenheilkunde. Interdisziplinäre Zusammenarbeit rund um die Augengesundheit. Am Beispiel von Nierenversagen

    Freitag, Randy

    Der Heilpraktiker

    2022  Volume 89, Issue 9, Page(s) 44

    Language German
    Document type Article
    ZDB-ID 2588428-1
    ISSN 2751-1596
    Database Current Contents Medicine

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  2. Article ; Online: Influence of NH

    Mlinar, Stanislava / Weig, Alfons R / Freitag, Ruth

    Bioresource technology

    2022  Volume 361, Page(s) 127638

    Abstract: Despite the extensive research dedicated to ammonia inhibition, the effect of ... ...

    Abstract Despite the extensive research dedicated to ammonia inhibition, the effect of NH
    MeSH term(s) Ammonia/pharmacology ; Anaerobiosis ; Bacteria ; Biofuels ; Bioreactors/microbiology ; Nitrogen ; Triacetoneamine-N-Oxyl
    Chemical Substances Biofuels ; Triacetoneamine-N-Oxyl (2896-70-0) ; Ammonia (7664-41-7) ; Nitrogen (N762921K75)
    Language English
    Publishing date 2022-07-16
    Publishing country England
    Document type Journal Article
    ZDB-ID 1065195-0
    ISSN 1873-2976 ; 0960-8524
    ISSN (online) 1873-2976
    ISSN 0960-8524
    DOI 10.1016/j.biortech.2022.127638
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  3. Article ; Online: Optimization of soil microbial fuel cell for sustainable bio-electricity production: combined effects of electrode material, electrode spacing, and substrate feeding frequency on power generation and microbial community diversity.

    Simeon, Imologie Meshack / Weig, Alfons / Freitag, Ruth

    Biotechnology for biofuels and bioproducts

    2022  Volume 15, Issue 1, Page(s) 124

    Abstract: Background: Microbial fuel cells (MFCs) are among the leading research topics in the field of alternative energy sources due to their multifunctional potential. However, their low bio-energy production rate and unstable performance limit their ... ...

    Abstract Background: Microbial fuel cells (MFCs) are among the leading research topics in the field of alternative energy sources due to their multifunctional potential. However, their low bio-energy production rate and unstable performance limit their application in the real world. Therefore, optimization is needed to deploy MFCs beyond laboratory-scale experiments. In this study, we investigated the combined influence of electrode material (EM), electrode spacing (ES), and substrate feeding interval (SFI) on microbial community diversity and the electrochemical behavior of a soil MFC (S-MFC) for sustainable bio-electricity generation.
    Results: Two EMs (carbon felt (CF) and stainless steel/epoxy/carbon black composite (SEC)) were tested in an S-MFC under three levels of ES (2, 4, and 8 cm) and SFI (4, 6, and 8 days). After 30 days of operation, all MFCs achieved open-circuit voltage in the range of 782 + 12.2 mV regardless of the treatment. However, the maximum power of the SEC-MFC was 3.6 times higher than that of the CF-MFC under the same experimental conditions. The best solution, based on the interactive influence of the two discrete variables, was obtained with SEC at an ES of 4.31 cm and an SFI of 7.4 days during an operating period of 66 days. Analysis of the experimental treatment effects of the variables revealed the order SFI < ES < EM, indicating that EM is the most influential factor affecting the performance of S-MFC. The performance of S-MFC at a given ES value was found to be dependent on the levels of SFI with the SEC electrode, but this interactive influence was found to be insignificant with the CF electrode. The microbial bioinformatic analysis of the samples from the S-MFCs revealed that both electrodes (SEC and CF) supported the robust metabolism of electroactive microbes with similar morphological and compositional characteristics, independent of ES and SFI. The complex microbial community showed significant compositional changes at the anode and cathode over time.
    Conclusion: This study has demonstrated that the performance of S-MFC depends mainly on the electrode materials and not on the diversity of the constituent microbial communities. The performance of S-MFCs can be improved using electrode materials with pseudocapacitive properties and a larger surface area, instead of using unmodified CF electrodes commonly used in S-MFC systems.
    Language English
    Publishing date 2022-11-16
    Publishing country England
    Document type Journal Article
    ISSN 2731-3654
    ISSN (online) 2731-3654
    DOI 10.1186/s13068-022-02224-9
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  4. Article ; Conference proceedings ; Online: Biosensor Systems for Process Control in Biotechnolgy

    Scheper, Th. / Anders, K. D. / Freitag, R. / Hundeck, H. G. / Müller, W. / Schelp, C. / Bückmann, A. F. / Reardon, K. F.

    2024  

    Abstract: Three different types of biosensor systems will be presented in this paper: a microbial optrode (measuring the intracellular NAD(P)H-content in immobilized cells), an optrode with immobilized coenzymes and enzymes (measuring the fluorescence of molecular ...

    Abstract Three different types of biosensor systems will be presented in this paper: a microbial optrode (measuring the intracellular NAD(P)H-content in immobilized cells), an optrode with immobilized coenzymes and enzymes (measuring the fluorescence of molecular weight increased NADH/NADPH), an enzymethermistor system (measuring the heat evolved during enzymatic or microbial reactions) and a automized immunoanalysis system. The application of these sensor systemswill be discussed.
    Language English
    Publishing date 2024-01-16
    Publisher GBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweig
    Publishing country de
    Document type Article ; Conference proceedings ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Book ; Thesis: Untersuchungen zum Einfluß ausgewählter Harnparameter auf das Kristallwachstum von Kalziumoxalat in einem neuen Flußmodell der Kristallisation in Gelmatrizes

    Freitag, Rainer

    1995  

    Author's details vorgelegt von Rainer Freitag, geb. Kothe
    Language German
    Size 79 S. : Ill., graph. Darst.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Marburg, Univ., Diss., 1996
    HBZ-ID HT007265150
    Database Catalogue ZB MED Medicine, Health

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    Shelf markLoan statusLocation
    96 D 2705 order for loan Magazin

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  6. Article ; Online: Data mining antibody sequences for database searching in bottom-up proteomics

    Trinh, Xuan-Tung / Freitag, Rebecca / Krawczyk, Konrad / Schwammle, Veit

    bioRxiv

    Abstract: Mass spectrometry (MS)-based proteomics allows identifying and quantifying thousands of proteins but suffers from challenges when measuring human antibodies due to their vast variety. The mainly used bottom-up proteomics approaches rely on database ... ...

    Abstract Mass spectrometry (MS)-based proteomics allows identifying and quantifying thousands of proteins but suffers from challenges when measuring human antibodies due to their vast variety. The mainly used bottom-up proteomics approaches rely on database searches that compare experimental values of peptides and their fragments to theoretical values derived from protein sequences in a database. While the human body can produce millions of distinct antibodies, the current databases for human antibodies such as UniProtKB/Swiss-Prot are limited to only 1095 sequences (as of 2024 Jan). This limitation may hinder the identification of new antibodies using mass spectrometry. Therefore, extending the database for mass spectrometry is an important task for discovering new antibodies. Recent genomic studies have compiled millions of human antibody sequences publicly accessible through the Observed Antibody Space (OAS) database. However, this data has yet to be exploited to confirm the presence of these antibodies. In this study, we adopted this extensive collection of antibody sequences for conducting efficient database searches in publicly available proteomics data with a focus on the SARS-CoV-2 disease. Thirty million heavy antibody sequences from 146 SARS-CoV-2 patients in the OAS database were digested in silico to obtain 18 million unique peptides. These peptides were then used to create new databases for bottom-up proteomics. We used those databases for searching new antibody peptides in publicly available SARS-CoV-2 human plasma samples in the Proteomics Identification Database (PRIDE). This approach avoids false positives in antibody peptide identification as confirmed by searching against negative controls (brain samples) and employing different database sizes. We show that the found sequences provide valuable information to distinguish diseased from healthy and expect that the newly discovered antibody peptides can be further employed to develop therapeutic antibodies. The method will be broadly applicable to find characteristic antibodies for other diseases.
    Keywords covid19
    Language English
    Publishing date 2024-02-14
    Publisher Cold Spring Harbor Laboratory
    Document type Article ; Online
    DOI 10.1101/2024.02.13.580076
    Database COVID19

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  7. Article ; Online: Data mining antibody sequences for database searching in bottom-up proteomics

    Trinh, Xuan-Tung / Freitag, Rebecca / Krawczyk, Konrad / Schwämmle, Veit

    bioRxiv

    Abstract: Mass spectrometry (MS)-based proteomics allows identifying and quantifying thousands of proteins but suffers from challenges when measuring human antibodies due to their vast variety. The mainly used bottom-up proteomics approaches rely on database ... ...

    Abstract Mass spectrometry (MS)-based proteomics allows identifying and quantifying thousands of proteins but suffers from challenges when measuring human antibodies due to their vast variety. The mainly used bottom-up proteomics approaches rely on database searches that compare experimental values of peptides and their fragments to theoretical values derived from protein sequences in a database. While the human body can produce millions of distinct antibodies, the current databases for human antibodies such as UniProtKB/Swiss-Prot are limited to only 1095 sequences (as of 2024 Jan). This limitation may hinder the identification of new antibodies using mass spectrometry. Therefore, extending the database for mass spectrometry is an important task for discovering new antibodies. Recent genomic studies have compiled millions of human antibody sequences publicly accessible through the Observed Antibody Space (OAS) database. However, this data has yet to be exploited to confirm the presence of these antibodies. In this study, we adopted this extensive collection of antibody sequences for conducting efficient database searches in publicly available proteomics data with a focus on the SARS-CoV-2 disease. Thirty million heavy antibody sequences from 146 SARS-CoV-2 patients in the OAS database were digested in silico to obtain 18 million unique peptides. These peptides were then used to create new databases for bottom-up proteomics. We used those databases for searching new antibody peptides in publicly available SARS-CoV-2 human plasma samples in the Proteomics Identification Database (PRIDE). This approach avoids false positives in antibody peptide identification as confirmed by searching against negative controls (brain samples) and employing different database sizes. We show that the found sequences provide valuable information to distinguish diseased from healthy and expect that the newly discovered antibody peptides can be further employed to develop therapeutic antibodies. The method will be broadly applicable to find characteristic antibodies for other diseases.
    Keywords covid19
    Language English
    Publishing date 2024-02-14
    Publisher Cold Spring Harbor Laboratory
    Document type Article ; Online
    DOI 10.1101/2024.02.13.580076
    Database COVID19

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  8. Article ; Online: Chromatographic techniques in the downstream processing of proteins in biotechnology.

    Freitag, Ruth

    Methods in molecular biology (Clifton, N.J.)

    2014  Volume 1104, Page(s) 419–458

    Abstract: The purification of the product, the so-called downstream process (DSP), tends to be one of the most costly aspects of modern bioprocessing, especially in the case of proteins. In such cases, chromatography is still the major tool on all levels of the ... ...

    Abstract The purification of the product, the so-called downstream process (DSP), tends to be one of the most costly aspects of modern bioprocessing, especially in the case of proteins. In such cases, chromatography is still the major tool on all levels of the DSP from the first capture to the final polishing step. In this chapter, we will first outline the commonly used methods and their setup, in particular ion exchange chromatography (IEX), hydrophobic interaction chromatography (HIC), affinity chromatography (AC), and gel filtration (GPC, SEC), but also some less-known alternatives such as hydroxyapatite chromatography (HAC). Then the rational design of a downstream process, which usually comprises three orthogonal chromatographic steps, is discussed. Finally, process variants deviating from the usual batch-column/gradient elution approach will be presented, including expanded bed, displacement, and continuous chromatography, but also affinity precipitation. A most recent trend observable in the biotechnical DSP is the drive towards disposable elements (single-use technologies). Some options for this will be discussed as well.
    MeSH term(s) Biotechnology/methods ; Chromatography/instrumentation ; Chromatography/methods ; Chromatography, Affinity/methods ; Chromatography, Gel/methods ; Chromatography, Ion Exchange/methods ; Equipment Design ; Recombinant Proteins/isolation & purification
    Chemical Substances Recombinant Proteins
    Language English
    Publishing date 2014
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-62703-733-4_25
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Isolation of primary human B lymphocytes from tonsils compared to blood as alternative source for ex vivo application.

    Helm, M / A B Riedl, S / Gollner, K / Gollner, U / Jérôme, V / Freitag, R

    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    2021  Volume 1179, Page(s) 122853

    Abstract: B lymphocytes ('B cells') are components of the human immune system with obvious potential for medical and biotechnological applications. Here, we discuss the isolation of primary human B cells from both juvenile and adult tonsillar material using a two- ... ...

    Abstract B lymphocytes ('B cells') are components of the human immune system with obvious potential for medical and biotechnological applications. Here, we discuss the isolation of primary human B cells from both juvenile and adult tonsillar material using a two-step procedure based on gradient centrifugation followed by separation on a nylon wool column as alternative to the current gold standard, i.e., negative immunosorting from buffy coats by antibody-coated magnetic beads. We show that the nylon wool separation is a low-cost method well suited to the isolation of large amounts of primary B cells reaching purities ≥ 80%. More importantly, this method allows the preservation of all B cell subsets, while MACS sorting seems to be biased against a certain B cell subtype, namely the CD27
    MeSH term(s) Adult ; B-Lymphocytes/classification ; B-Lymphocytes/cytology ; B-Lymphocytes/drug effects ; Cell Culture Techniques ; Cell Proliferation/drug effects ; Cell Separation/methods ; Cell Separation/standards ; Cells, Cultured ; Centrifugation ; Child ; Humans ; Nylons ; Palatine Tonsil/cytology ; Pokeweed Mitogens/pharmacology
    Chemical Substances Nylons ; Pokeweed Mitogens
    Language English
    Publishing date 2021-07-07
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1180823-8
    ISSN 1873-376X ; 0378-4347 ; 1570-0232 ; 1387-2273
    ISSN (online) 1873-376X
    ISSN 0378-4347 ; 1570-0232 ; 1387-2273
    DOI 10.1016/j.jchromb.2021.122853
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Digitalisierung als Chance

    Freitag, Robert

    Aspekte der Digitalisierung in Banken , p. 21-27

    2017  , Page(s) 21–27

    Author's details Robert Freitag
    Keywords Fintech ; Electronic Banking ; Bankgeschäft ; Wettbewerbsstrategie ; Bank
    Language German
    Publisher Bank-Verlag GmbH
    Publishing place Köln
    Document type Article
    ISBN 978-3-86556-484-9 ; 3-86556-484-4
    Database ECONomics Information System

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