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  1. Article ; Online: Pregnant women's attitudes and behaviours towards antenatal vaccination against Influenza and COVID-19 in the Liverpool City Region, United Kingdom: Cross-sectional survey.

    Kilada, Samantha / French, Neil / Perkins, Elizabeth / Hungerford, Dan

    Vaccine: X

    2023  Volume 15, Page(s) 100387

    Abstract: Objectives: Influenza poses a serious health risk to pregnant women and their babies. Despite this risk, influenza vaccine uptake in pregnant women in the UK is less than 50%. Little is known about how COVID-19 affects pregnant women, but its management ...

    Abstract Objectives: Influenza poses a serious health risk to pregnant women and their babies. Despite this risk, influenza vaccine uptake in pregnant women in the UK is less than 50%. Little is known about how COVID-19 affects pregnant women, but its management may affect attitudes and behaviours towards vaccination in pregnancy. The study objectives were to establish attitudes and knowledge of pregnant women towards influenza disease and influenza vaccination and to compare these to attitudes and knowledge about COVID-19 and COVID-19 vaccination.
    Design: A cross-sectional survey was conducted using an online questionnaire distributed through local advertisement and social media outlets. Information was sought on attitudes and knowledge of influenza and COVID-19 and their respective vaccines.
    Participants and setting: Pregnant women residing in Liverpool City Region, UK.
    Results: Of the 237 respondents, 73.8% reported receiving an influenza vaccine. Over half (56.5%) perceived themselves to be at risk from influenza, 70.5% believed that if they got influenza, their baby would get ill, and 64.6% believed getting influenza could hurt their baby, 60.3% believed that the influenza vaccine would prevent their baby from getting ill, and 70.8% believed it would protect their baby. Only 32.9% of respondents stated they would receive the COVID-19 vaccine if it were available to them. However, 80.2% stated they would receive a COVID-19 vaccine if they were not pregnant. Most of the women stated that they would accept a vaccine if recommended to them by healthcare professionals.
    Conclusions: Acceptance of the influenza and COVID-19 vaccines during pregnancy seems to be more related to the safety of the baby rather than the mother. Women perceived their child to be more at risk than themselves. Information about influenza and COVID-19 vaccine safety as well as healthcare provider recommendations play an important role in vaccine uptake in pregnant women.
    Language English
    Publishing date 2023-09-16
    Publishing country England
    Document type Journal Article
    ISSN 2590-1362
    ISSN (online) 2590-1362
    DOI 10.1016/j.jvacx.2023.100387
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Pregnant women's attitudes and behaviours towards antenatal vaccination against Influenza and COVID-19 in the Liverpool City Region, United Kingdom: cross-sectional survey

    Kilada, Samantha / French, Neil / Perkins, Elizabeth / Hungerford, Daniel

    medRxiv

    Abstract: Objectives Influenza poses a serious health risk to pregnant women and their babies. Despite this risk, influenza vaccine uptake in pregnant women in the UK is less than 50%. Little is known about how COVID-19 affects pregnant women, but its management ... ...

    Abstract Objectives Influenza poses a serious health risk to pregnant women and their babies. Despite this risk, influenza vaccine uptake in pregnant women in the UK is less than 50%. Little is known about how COVID-19 affects pregnant women, but its management may affect attitudes and behaviours towards vaccination in pregnancy. The study objectives were to establish attitudes and knowledge of pregnant women towards influenza disease and influenza vaccination and to compare these to attitudes and knowledge about COVID-19 and COVID-19 vaccination. Design A cross-sectional survey was conducted using an online questionnaire distributed through local advertisement and social media outlets. Information was sought on attitudes and knowledge of influenza and COVID-19 and their respective vaccines. Participants and setting Pregnant women residing in Liverpool City Region, UK Results Of the 237 respondents, 73.8% reported receiving an influenza vaccine. Over half (56.5%) perceived themselves to be at risk from influenza, 70.5% believed that if they got influenza, their baby would get ill, and 64.6% believed getting influenza could hurt their baby, 60.3% believed that the influenza vaccine would prevent their baby from getting ill, and 70.8% believed it would protect their baby. Only 32.9% of respondents stated they would receive the COVID-19 vaccine if it were available to them. However, 80.2% stated they would receive a COVID-19 vaccine if they were not pregnant. Most of the women stated that they would accept a vaccine if recommended to them by healthcare professionals. Conclusions Acceptance of the influenza and COVID-19 vaccines during pregnancy seems to be more related to the safety of the baby rather than the mother. Women perceived their child to be more at risk than themselves. Information about influenza and COVID-19 vaccine safety as well as healthcare provider recommendations play an important role in vaccine uptake in pregnant women.
    Keywords covid19
    Language English
    Publishing date 2022-09-14
    Publisher Cold Spring Harbor Laboratory Press
    Document type Article ; Online
    DOI 10.1101/2022.09.13.22279846
    Database COVID19

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  3. Article ; Online: Mechanistic Insights into the Impact of Air Pollution on Pneumococcal Pathogenesis and Transmission.

    Beentjes, Daan / Shears, Rebecca K / French, Neil / Neill, Daniel R / Kadioglu, Aras

    American journal of respiratory and critical care medicine

    2022  Volume 206, Issue 9, Page(s) 1070–1080

    Abstract: Streptococcus ... ...

    Abstract Streptococcus pneumoniae
    MeSH term(s) Humans ; Streptococcus pneumoniae ; Air Pollution/analysis ; Air Pollutants/analysis ; Particulate Matter/analysis ; Pneumonia/epidemiology ; Pneumococcal Infections/complications
    Chemical Substances Air Pollutants ; Particulate Matter
    Language English
    Publishing date 2022-05-27
    Publishing country United States
    Document type Review ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1180953-x
    ISSN 1535-4970 ; 0003-0805 ; 1073-449X
    ISSN (online) 1535-4970
    ISSN 0003-0805 ; 1073-449X
    DOI 10.1164/rccm.202112-2668TR
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online ; Conference proceedings: Biomarkers - SMi's Third Summit: discovery, validation & qualification.

    French, Neil

    IDrugs : the investigational drugs journal

    2010  Volume 13, Issue 4, Page(s) 204–208

    Abstract: SMi's Third Summit on Biomarkers, held in London, included topics covering new technological and therapeutic developments in the field of biomarkers. This conference report highlights selected presentations on the development of biomarkers, novel ... ...

    Abstract SMi's Third Summit on Biomarkers, held in London, included topics covering new technological and therapeutic developments in the field of biomarkers. This conference report highlights selected presentations on the development of biomarkers, novel biomarkers for cancer and other diseases, and safety issues relating to biomarkers.
    MeSH term(s) Alzheimer Disease/cerebrospinal fluid ; Alzheimer Disease/diagnosis ; Animals ; Biomarkers/analysis ; Biomarkers/blood ; Biomarkers/cerebrospinal fluid ; Biomarkers/metabolism ; Biomarkers/urine ; Biomarkers, Pharmacological/analysis ; Biomarkers, Pharmacological/blood ; Biomarkers, Pharmacological/metabolism ; Biomarkers, Pharmacological/urine ; Carcinogenicity Tests/methods ; Chemical and Drug Induced Liver Injury/blood ; Chemical and Drug Induced Liver Injury/metabolism ; Drug-Related Side Effects and Adverse Reactions/blood ; Drug-Related Side Effects and Adverse Reactions/metabolism ; Drug-Related Side Effects and Adverse Reactions/urine ; Humans ; Kidney Diseases/chemically induced ; Kidney Diseases/metabolism ; Kidney Diseases/urine ; Legislation as Topic ; Lupus Erythematosus, Systemic/metabolism ; Male ; Neoplasms/diagnosis ; Neoplasms/immunology ; Neoplasms/metabolism ; Prostatic Neoplasms/diagnosis ; Prostatic Neoplasms/metabolism ; Quality Control
    Chemical Substances Biomarkers ; Biomarkers, Pharmacological
    Language English
    Publishing date 2010-04
    Publishing country England
    Document type Congresses
    ZDB-ID 2086568-5
    ISSN 2040-3410 ; 1369-7056
    ISSN (online) 2040-3410
    ISSN 1369-7056
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: TNFR2

    Xu, Rong / Jacques, Laura C / Khandaker, Shadia / Beentjes, Daan / Leon-Rios, Miguel / Wei, Xiaoqing / French, Neil / Neill, Daniel R / Kadioglu, Aras

    Cell reports

    2023  Volume 42, Issue 2, Page(s) 112054

    Abstract: Streptococcus pneumoniae is a pathogen of global morbidity and mortality. Pneumococcal pneumonia can lead to systemic infections associated with high rates of mortality. We find that, upon pneumococcal infection, pulmonary Treg cells are activated and ... ...

    Abstract Streptococcus pneumoniae is a pathogen of global morbidity and mortality. Pneumococcal pneumonia can lead to systemic infections associated with high rates of mortality. We find that, upon pneumococcal infection, pulmonary Treg cells are activated and have upregulated TNFR2 expression. TNFR2-deficient mice have compromised Treg cell responses and highly activated IL-17A-producing γδ T cell (γδT17) responses, resulting in significantly enhanced neutrophil infiltration, tissue damage, and rapid development of bacteremia, mirroring responses in Treg cell-depleted mice. Deletion of total Treg cells predominantly activate IFNγ-T cell responses, whereas adoptive transfer of TNFR2
    MeSH term(s) Mice ; Animals ; Pneumonia, Pneumococcal/metabolism ; T-Lymphocytes, Regulatory/metabolism ; Interleukin-17/metabolism ; Receptors, Tumor Necrosis Factor, Type II ; Lung/metabolism ; Bacteremia ; Mice, Inbred C57BL ; Receptors, Antigen, T-Cell, gamma-delta/metabolism
    Chemical Substances Interleukin-17 ; Receptors, Tumor Necrosis Factor, Type II ; Receptors, Antigen, T-Cell, gamma-delta
    Language English
    Publishing date 2023-01-30
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2649101-1
    ISSN 2211-1247 ; 2211-1247
    ISSN (online) 2211-1247
    ISSN 2211-1247
    DOI 10.1016/j.celrep.2023.112054
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: The seroprevalence of SARS-CoV-2 during the first wave in Europe 2020: A systematic review.

    Vaselli, Natasha Marcella / Hungerford, Daniel / Shenton, Ben / Khashkhusha, Arwa / Cunliffe, Nigel A / French, Neil

    PloS one

    2021  Volume 16, Issue 11, Page(s) e0250541

    Abstract: Background: A year following the onset of the COVID-19 pandemic, new infections and deaths continue to increase in Europe. Serological studies, through providing evidence of past infection, can aid understanding of the population dynamics of SARS-CoV-2 ... ...

    Abstract Background: A year following the onset of the COVID-19 pandemic, new infections and deaths continue to increase in Europe. Serological studies, through providing evidence of past infection, can aid understanding of the population dynamics of SARS-CoV-2 infection.
    Objectives: This systematic review of SARS-CoV-2 seroprevalence studies in Europe was undertaken to inform public health strategies including vaccination, that aim to accelerate population immunity.
    Methods: We searched the databases Web of Science, MEDLINE, EMBASE, SCOPUS, Cochrane Database of Systematic Reviews and grey literature sources for studies reporting seroprevalence of SARS-CoV-2 antibodies in Europe published between 01/12/2019-30/09/20. We provide a narrative synthesis of included studies. Studies were categorized into subgroups including healthcare workers (HCWs), community, outbreaks, pregnancy and children/school. Due to heterogeneity in other subgroups, we only performed a random effects meta-analysis of the seroprevalence amongst HCWs stratified by their country.
    Results: 115 studies were included spanning 17 European countries, that estimated the seroprevalence of SARS-CoV-2 from samples obtained between November 2019 -August 2020. A total of 54/115 studies included HCWs with a reported seroprevalence among HCWs ranging from 0.7% to 45.3%, which did not differ significantly by country. In community studies significant heterogeneity was reported in the seroprevalence between different age groups and the majority of studies reported there was no significant difference by gender.
    Conclusion: This review demonstrates a wide heterogeneity in reported seroprevalence of SARS-CoV-2 antibodies between populations. Continued evaluation of seroprevalence is required to understand the impact of public health measures and inform interventions including vaccination programmes.
    MeSH term(s) COVID-19/epidemiology ; COVID-19/immunology ; COVID-19/prevention & control ; Europe/epidemiology ; Humans ; Pandemics ; Public Health ; Seroepidemiologic Studies
    Language English
    Publishing date 2021-11-02
    Publishing country United States
    Document type Journal Article ; Systematic Review
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0250541
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Investigating dihydroorotate dehydrogenase inhibitor mediated mitochondrial dysfunction in hepatic in vitro models.

    Jones, Samantha W / Penman, Sophie L / French, Neil S / Park, B Kevin / Chadwick, Amy E

    Toxicology in vitro : an international journal published in association with BIBRA

    2021  Volume 72, Page(s) 105096

    Abstract: Inhibition of dihydroorotate dehydrogenase (DHODH), the rate-limiting enzymatic step in de novo pyrimidine synthesis, has broad immunosuppressive effects in vivo and shows promise as a therapeutic target for the treatment of malignancies, viral ... ...

    Abstract Inhibition of dihydroorotate dehydrogenase (DHODH), the rate-limiting enzymatic step in de novo pyrimidine synthesis, has broad immunosuppressive effects in vivo and shows promise as a therapeutic target for the treatment of malignancies, viral infections and auto-immune diseases. Whilst there are numerous DHODH inhibitors under development, leflunomide and teriflunomide are the only FDA approved compounds on the market, each of which have been issued with black-box warnings for hepatotoxicity. Mitochondrial dysfunction is a putative mechanism by which teriflunomide and leflunomide elicit their hepatotoxic effects, however it is as yet unclear whether this is shared by other nascent DHODH inhibitors. The present study aimed to evaluate the propensity for DHODH inhibitors to mediate mitochondrial dysfunction in two hepatic in vitro models. Initial comparisons of cytotoxicity and ATP content in HepaRG® cells primed for oxidative metabolism, in tandem with mechanistic evaluations by extracellular flux analysis identified multifactorial toxicity and moderate indications of respiratory chain dysfunction or uncoupling. Further investigations using HepG2 cells, a hepatic line with limited capability for phase I xenobiotic metabolism, identified leflunomide and brequinar as positive mitochondrial toxicants. Taken together, biotransformation of some DHODH inhibitor species may play a role in mediating or masking hepatic mitochondrial liabilities.
    MeSH term(s) Adenosine Triphosphate/metabolism ; Antineoplastic Agents/toxicity ; Biphenyl Compounds/toxicity ; Cell Line ; Cell Respiration/drug effects ; Crotonates/toxicity ; Dicarboxylic Acids/toxicity ; Humans ; Hydroxybutyrates/toxicity ; Immunosuppressive Agents/toxicity ; Leflunomide/toxicity ; Liver/drug effects ; Liver/metabolism ; Mitochondria/drug effects ; Mitochondria/metabolism ; Models, Biological ; Nitriles/toxicity ; Oxidoreductases Acting on CH-CH Group Donors/antagonists & inhibitors ; Salicylanilides/toxicity ; Toluidines/toxicity ; Triazoles/toxicity
    Chemical Substances Antineoplastic Agents ; Biphenyl Compounds ; Crotonates ; Dicarboxylic Acids ; Hydroxybutyrates ; Immunosuppressive Agents ; Nitriles ; Salicylanilides ; Toluidines ; Triazoles ; teriflunomide (1C058IKG3B) ; brequinar (5XL19F49H6) ; Adenosine Triphosphate (8L70Q75FXE) ; vidofludimus (8Y1PJ3VG81) ; Oxidoreductases Acting on CH-CH Group Donors (EC 1.3.-) ; dihydroorotate dehydrogenase (EC 1.3.5.2) ; Leflunomide (G162GK9U4W) ; orludodstat (X8GF945GMK)
    Language English
    Publishing date 2021-01-16
    Publishing country England
    Document type Journal Article
    ZDB-ID 639064-x
    ISSN 1879-3177 ; 0887-2333
    ISSN (online) 1879-3177
    ISSN 0887-2333
    DOI 10.1016/j.tiv.2021.105096
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Epidemiology of Human Seasonal Coronaviruses Among People With Mild and Severe Acute Respiratory Illness in Blantyre, Malawi, 2011-2017.

    Kovacs, Dory / Mambule, Ivan / Read, Jonathan M / Kiran, Anmol / Chilombe, Moses / Bvumbwe, Thandiwe / Aston, Stephen / Menyere, Mavis / Masina, Mazuba / Kamzati, Moses / Ganiza, Thokozani Namale / Iuliano, Danielle / McMorrow, Meredith / Bar-Zeev, Naor / Everett, Dean / French, Neil / Ho, Antonia

    The Journal of infectious diseases

    2024  

    Abstract: Background: The aim of this study was to characterize the epidemiology of human seasonal coronaviruses (HCoVs) in southern Malawi.: Methods: We tested for HCoVs 229E, OC43, NL63, and HKU1 using real-time polymerase chain reaction (PCR) on upper ... ...

    Abstract Background: The aim of this study was to characterize the epidemiology of human seasonal coronaviruses (HCoVs) in southern Malawi.
    Methods: We tested for HCoVs 229E, OC43, NL63, and HKU1 using real-time polymerase chain reaction (PCR) on upper respiratory specimens from asymptomatic controls and individuals of all ages recruited through severe acute respiratory illness (SARI) surveillance at Queen Elizabeth Central Hospital, Blantyre, and a prospective influenza-like illness (ILI) observational study between 2011 and 2017. We modeled the probability of having a positive PCR for each HCoV using negative binomial models, and calculated pathogen-attributable fractions (PAFs).
    Results: Overall, 8.8% (539/6107) of specimens were positive for ≥1 HCoV. OC43 was the most frequently detected HCoV (3.1% [191/6107]). NL63 was more frequently detected in ILI patients (adjusted incidence rate ratio [aIRR], 9.60 [95% confidence interval {CI}, 3.25-28.30]), while 229E (aIRR, 8.99 [95% CI, 1.81-44.70]) was more frequent in SARI patients than asymptomatic controls. In adults, 229E and OC43 were associated with SARI (PAF, 86.5% and 89.4%, respectively), while NL63 was associated with ILI (PAF, 85.1%). The prevalence of HCoVs was similar between children with SARI and controls. All HCoVs had bimodal peaks but distinct seasonality.
    Conclusions: OC43 was the most prevalent HCoV in acute respiratory illness of all ages. Individual HCoVs had distinct seasonality that differed from temperate settings.
    Language English
    Publishing date 2024-02-14
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3019-3
    ISSN 1537-6613 ; 0022-1899
    ISSN (online) 1537-6613
    ISSN 0022-1899
    DOI 10.1093/infdis/jiad587
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Clonal Expansion of a Streptococcus pneumoniae Serotype 3 Capsule Variant Sequence Type 700 With Enhanced Vaccine Escape Potential After 13-Valent Pneumococcal Conjugate Vaccine Introduction.

    Kalizang'oma, Akuzike / Swarthout, Todd D / Mwalukomo, Thandie S / Kamng'ona, Arox / Brown, Comfort / Msefula, Jacquline / Demetriou, Hayley / Chan, Jia Mun / Roalfe, Lucy / Obolski, Uri / Lourenço, Jose / Goldblatt, David / Chaguza, Chrispin / French, Neil / Heyderman, Robert S

    The Journal of infectious diseases

    2024  

    Abstract: Background: Streptococcus pneumoniae serotype 3 remains a problem globally. Malawi introduced 13-valent pneumococcal conjugate vaccine (PCV13) in 2011, but there has been no direct protection against serotype 3 carriage. We explored whether vaccine ... ...

    Abstract Background: Streptococcus pneumoniae serotype 3 remains a problem globally. Malawi introduced 13-valent pneumococcal conjugate vaccine (PCV13) in 2011, but there has been no direct protection against serotype 3 carriage. We explored whether vaccine escape by serotype 3 is due to clonal expansion of a lineage with a competitive advantage.
    Methods: The distribution of serotype 3 Global Pneumococcal Sequence Clusters (GPSCs) and sequence types (STs) globally was assessed using sequences from the Global Pneumococcal Sequencing Project. Whole-genome sequences of 135 serotype 3 carriage isolates from Blantyre, Malawi (2015-2019) were analyzed. Comparative analysis of the capsule locus, entire genomes, antimicrobial resistance, and phylogenetic reconstructions were undertaken. Opsonophagocytosis was evaluated using serum samples from vaccinated adults and children.
    Results: Serotype 3 GPSC10-ST700 isolates were most prominent in Malawi. Compared with the prototypical serotype 3 capsular polysaccharide locus sequence, 6 genes are absent, with retention of capsule polysaccharide biosynthesis. This lineage is characterized by increased antimicrobial resistance and lower susceptibility to opsonophagocytic killing.
    Conclusions: A serotype 3 variant in Malawi has genotypic and phenotypic characteristics that could enhance vaccine escape and clonal expansion after post-PCV13 introduction. Genomic surveillance among high-burden populations is essential to improve the effectiveness of next-generation pneumococcal vaccines.
    Language English
    Publishing date 2024-03-26
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3019-3
    ISSN 1537-6613 ; 0022-1899
    ISSN (online) 1537-6613
    ISSN 0022-1899
    DOI 10.1093/infdis/jiae040
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Vaccine strategies to reduce the burden of pneumococcal disease in HIV-infected adults in Africa.

    Thindwa, Deus / Pinsent, Amy / Ojal, John / Gallagher, Katherine E / French, Neil / Flasche, Stefan

    Expert review of vaccines

    2020  Volume 19, Issue 11, Page(s) 1085–1092

    Abstract: Introduction: Streptococcus pneumoniae: Areas covered: Nonsystematic review on the pneumococcal burden in HIV-infected adults and vaccine strategies to reduce this burden.: Expert opinion: We propose and discuss the relative merit of changing the ... ...

    Abstract Introduction: Streptococcus pneumoniae
    Areas covered: Nonsystematic review on the pneumococcal burden in HIV-infected adults and vaccine strategies to reduce this burden.
    Expert opinion: We propose and discuss the relative merit of changing the infant PCV program to use (1a) a two prime plus booster dose schedule, (1b) a two prime plus booster dose schedule with an additional booster dose at school entry, to directly vaccinate (2a) HIV-infected adults or vaccinating (2b) HIV-infected pregnant women for direct protection, with added indirect protection to the high-risk neonates. We identify key knowledge gaps for such an evaluation and propose strategies to overcome them.
    MeSH term(s) Adult ; Africa ; Anti-HIV Agents/administration & dosage ; HIV Infections/complications ; HIV Infections/drug therapy ; Humans ; Immunization Schedule ; Infant ; Pneumococcal Infections/prevention & control ; Pneumococcal Vaccines/administration & dosage ; Streptococcus pneumoniae/isolation & purification ; Vaccines, Conjugate/administration & dosage
    Chemical Substances Anti-HIV Agents ; Pneumococcal Vaccines ; Vaccines, Conjugate
    Language English
    Publishing date 2020-12-03
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2181284-6
    ISSN 1744-8395 ; 1476-0584
    ISSN (online) 1744-8395
    ISSN 1476-0584
    DOI 10.1080/14760584.2020.1843435
    Database MEDical Literature Analysis and Retrieval System OnLINE

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