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  1. Article ; Online: Paediatric reference ranges for plasma chromogranin A.

    Lieberoth, Sofie / Friis-Hansen, Frederik / Friis-Hansen, Lennart

    Annals of clinical biochemistry

    2023  , Page(s) 45632231202652

    Abstract: Background: Neuroendocrine neoplasms (NENs) are a heterogeneous group of rare diseases with varied aggressiveness originating from endocrine cells belonging to the diffuse endocrine system and most often produce and secrete chromogranin A (CgA). CgA in ... ...

    Abstract Background: Neuroendocrine neoplasms (NENs) are a heterogeneous group of rare diseases with varied aggressiveness originating from endocrine cells belonging to the diffuse endocrine system and most often produce and secrete chromogranin A (CgA). CgA in plasma is therefore used to screen, diagnose, and monitor for NENs in both adults and children with sporadic or familial NENs.
    Methods: Plasma CgA was measured using the Brahms Kryptor assay in 268 healthy children/adolescents; 85 children were tested as part of a familial cancer screening program and 183 additional children younger than 20 years of age underwent screening for allergies. Repeated measurements (month - years) was used to calculate the intra-individual variation. The dataset was analysed in R using the referenceInterval package.
    Results: The plasma CgA concentration decreased with age and was 32-118 µg/L for children aged 0-3 years, 18-85 µg/L for children aged 4-13 years, and 6-79 µg/L for adolescents aged 14-19 years. Earlier reported CgA reference intervals for adults have upper limits from 88 to 102 µg/L while no lower limits have been reported. The median for the three groups were 78, 51, and 39 µg/L, respectively. The median intra-individual variation was 14% (25%-centile 9.4%/75%-centile 21%).
    Conclusions: The reference interval will be useful when screening, diagnosing, and monitoring children for NENs respecting the limitations plasma CgA has.
    Language English
    Publishing date 2023-09-15
    Publishing country England
    Document type Journal Article
    ZDB-ID 390309-6
    ISSN 1758-1001 ; 0004-5632
    ISSN (online) 1758-1001
    ISSN 0004-5632
    DOI 10.1177/00045632231202652
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  2. Book ; Thesis: Lessons from the gastrin knockout mice

    Friis-Hansen, Lennart

    2007  

    Author's details Lennart Friis-Hansen
    Language English
    Size 83 S., Ill., graph. Darst.
    Edition 1. udgave
    Publishing country Denmark
    Document type Book ; Thesis
    Thesis / German Habilitation thesis København, Univ., Diss., 2007
    HBZ-ID HT015377747
    ISBN 87-991392-0-0 ; 978-87-991392-0-0
    Database Catalogue ZB MED Medicine, Health

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    2007 E 2598 order for loan Magazin

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  3. Article ; Online: Collagenase-producing bacteria are common in anastomotic leakage after colorectal surgery: a systematic review.

    Jørgensen, Anders Bech / Jonsson, Isabella / Friis-Hansen, Lennart / Brandstrup, Birgitte

    International journal of colorectal disease

    2023  Volume 38, Issue 1, Page(s) 275

    Abstract: Purpose: Some gut bacteria can produce enzymes (collagenases) that can break down collagen in the intestinal wall. This could be a part of the pathophysiology of anastomotic leakage (AL). This systematic review aimed to investigate if such bacteria were ...

    Abstract Purpose: Some gut bacteria can produce enzymes (collagenases) that can break down collagen in the intestinal wall. This could be a part of the pathophysiology of anastomotic leakage (AL). This systematic review aimed to investigate if such bacteria were present more frequently in AL patients versus non-AL patients following colorectal surgery.
    Methods: This systematic review was reported according to the PRISMA and AMSTAR guidelines. Before the literature search, a study protocol was registered at PROSPERO (CRD42022363454). We searched PubMed, EMBASE, Google Scholar, and Cochrane CENTRAL on April 9
    Results: We included 15 studies, with a total of 52,945 patients, of which 1,747 had AL, and bacteriological information from feces, mucosa, the resected specimen, or drain fluid was presented. In 10 of the 15 studies, one or more collagenase-producing bacteria were identified in the patients with AL. Neither the bacteria nor the collagenase production were quantified in any of the studies. The studies varied greatly in terms of sample material, analytical method, and time of collection. Studies using DNA sequencing methods did not report findings of collagenase-producing bacteria.
    Conclusion: Collagenase-producing bacteria are more common in patients with AL following colorectal surgery than in patients without AL, but the significance is unclear. From the current studies, it is not possible to determine the pathogenicity of the individual gut bacteria.
    MeSH term(s) Humans ; Anastomotic Leak/etiology ; Colorectal Surgery/adverse effects ; Digestive System Surgical Procedures ; Collagenases ; Bacteria
    Chemical Substances Collagenases (EC 3.4.24.-)
    Language English
    Publishing date 2023-12-01
    Publishing country Germany
    Document type Systematic Review ; Journal Article ; Review
    ZDB-ID 84975-3
    ISSN 1432-1262 ; 0179-1958
    ISSN (online) 1432-1262
    ISSN 0179-1958
    DOI 10.1007/s00384-023-04562-y
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  4. Article ; Online: Validation of plasma thyroxine and triiodothyronine methods on the ADVIA Centaur

    Kristiansen, Søren / Friis-Hansen, Lennart

    Scandinavian journal of clinical and laboratory investigation

    2019  Volume 79, Issue 1-2, Page(s) 43–49

    Abstract: Standardization programs for thyroid hormones have revealed bias between immunochemical methods and the reference method ED-ID-LC/MS. Lack of standardization between methods, suboptimal reference intervals and replacement of serum with plasma may ... ...

    Abstract Standardization programs for thyroid hormones have revealed bias between immunochemical methods and the reference method ED-ID-LC/MS. Lack of standardization between methods, suboptimal reference intervals and replacement of serum with plasma may compromise the capability of the immunochemical thyroid methods to diagnose thyroid disease. To accommodate the demand for faster turn-around times for laboratory replies, we replaced serum with plasma on some serum CE marked thyroid methods. This forced us to do on-board analytical correction for the plasma total T4 (TT4) method on ADVIA Centaur
    MeSH term(s) Adolescent ; Adult ; Aged ; Automation, Laboratory/standards ; Biomarkers/blood ; Case-Control Studies ; Chromatography, Liquid/standards ; Female ; Humans ; Hyperthyroidism/blood ; Hyperthyroidism/diagnosis ; Hypothyroidism/blood ; Hypothyroidism/diagnosis ; Immunoassay/standards ; Male ; Mass Spectrometry/standards ; Middle Aged ; Reference Values ; Retrospective Studies ; Thyroxine/blood ; Triiodothyronine/blood
    Chemical Substances Biomarkers ; Triiodothyronine (06LU7C9H1V) ; Thyroxine (Q51BO43MG4)
    Language English
    Publishing date 2019-02-19
    Publishing country England
    Document type Journal Article ; Validation Studies
    ZDB-ID 3150-1
    ISSN 1502-7686 ; 0036-5513
    ISSN (online) 1502-7686
    ISSN 0036-5513
    DOI 10.1080/00365513.2019.1571624
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  5. Article ; Online: Varying fecal immunochemical test screening cutoffs by age and gender: a way to increase detection rates and reduce the number of colonoscopies.

    Njor, Sisse Helle / Rasmussen, Morten / Friis-Hansen, Lennart / Andersen, Berit

    Gastrointestinal endoscopy

    2021  Volume 95, Issue 3, Page(s) 540–549

    Abstract: Background and aims: Most colorectal cancer (CRC) screening programs based on the fecal immunochemical test (FIT) use the same cutoff value for all participants. This study aimed at finding age- and gender-specific cutoff values that can improve ... ...

    Abstract Background and aims: Most colorectal cancer (CRC) screening programs based on the fecal immunochemical test (FIT) use the same cutoff value for all participants. This study aimed at finding age- and gender-specific cutoff values that can improve population-based CRC screening.
    Methods: This observational study used data from the first 2 years of the Danish FIT-based CRC screening program to estimate sensitivity, specificity, number of positive tests, number of screen-detected cancers and adenomas, and number of interval cancers for various cutoff values for different male and female age groups.
    Results: Data from 531,828 participants showed that lower cutoff values for older residents and higher cutoff values for younger residents increased the overall sensitivity and specificity, decreased the number of needed colonoscopies by 7%, increased the number of screen-detected cancer by 1.1%, increased the number of screen-detected adenomas by 5%, and decreased the number of interval cancers by approximately 1.5%. However, these cutoff values also increased an inequality in sensitivity and specificity. Choosing cutoff values that ensured equal sensitivity between the groups, however, did increase inequality in, for example, the interval cancer rate.
    Conclusions: In a FIT-based CRC program it is possible to decrease the number of needed colonoscopies while at the same time to increase overall sensitivity and specificity and detect more cancers and adenomas by using different cutoff values for different male and female age groups. However, this increases inequality in sensitivity and specificity, whereas other strategies like ensuring equal sensitivity could be considered.
    MeSH term(s) Colonoscopy ; Colorectal Neoplasms/diagnosis ; Early Detection of Cancer ; Feces ; Female ; Humans ; Male ; Mass Screening ; Occult Blood
    Language English
    Publishing date 2021-10-06
    Publishing country United States
    Document type Journal Article ; Observational Study
    ZDB-ID 391583-9
    ISSN 1097-6779 ; 0016-5107
    ISSN (online) 1097-6779
    ISSN 0016-5107
    DOI 10.1016/j.gie.2021.09.038
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    Zs.MO 519: Show issues

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  6. Article ; Online: A novel hepcidin mutation.

    Praeger-Jahnsen, Louis / Magnussen, Karin / Schiødt, Frank Vinholt / Therkildsen, Rikke Christina / Jørgensen, Niels / Friis-Hansen, Lennart

    Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine

    2023  Volume 30, Issue 3, Page(s) 335–340

    Abstract: Background: The bioactive peptide hormone hepcidin-25 regulates iron levels by inhibiting iron transport to plasma via ferroportin. Hepcidin-25 is synthesized in the liver where the 84 amino acids pro-hepcidin is cleaved into the bioactive hepcidin-25. ... ...

    Abstract Background: The bioactive peptide hormone hepcidin-25 regulates iron levels by inhibiting iron transport to plasma via ferroportin. Hepcidin-25 is synthesized in the liver where the 84 amino acids pro-hepcidin is cleaved into the bioactive hepcidin-25. A patient admitted to the hospital presented with infertility and fatigue.
    Methods: Genomic DNA was purified from whole blood using the Maxwell 16 system (Promega). MLPA analysis was performed to detect large genomic rearrangements using the SALSA MLPA kit # P347, Hemochromatosis (MRC Holland, Holland). Plasma hepcidin measurements were performed using liquid chromatography/tandem mass spectrometry (LC-MS/MS).
    Results: A novel HAMP mutation (homozygous one base deletion in c.215delG, p.Cys72Serfs*?) was detected. The deletion in nucleotide 215 causes a frameshift altering the predicted protein sequence from cysteine13 in mature peptide. Whether this leads to nonsense mediated decay of the mRNA or synthesis of an aberrant peptide in unknown, but bioactive hepcidin-25 was undetectable in plasma. The patient had massive iron overload with ferritin up to 8360 µg/L. He was anaemic with a Hb at 7.0 mmol/L (11.3 g/dL) and suffered from hypogonadotropic hypogonadism with a total testosterone of 1.2 nmol/l. Continued treatment with venesection and gonadotropins led to reduced fatigue, reduction in iron overload, a normalized Hb and improvement of semen quality.
    Conclusion: A novel hepcidin mutation was detected in a patient with massive iron overload, fatigue and hypogonadotropic hypogonadism.
    MeSH term(s) Humans ; Hepcidins ; Iron/metabolism ; Chromatography, Liquid ; Semen Analysis ; Tandem Mass Spectrometry ; Iron Overload/genetics ; Iron Overload/metabolism ; Hypogonadism ; Mutation
    Chemical Substances Hepcidins ; Iron (E1UOL152H7)
    Language English
    Publishing date 2023-03-15
    Publishing country France
    Document type Journal Article
    ZDB-ID 1204698-x
    ISSN 1953-8022 ; 1246-7820
    ISSN (online) 1953-8022
    ISSN 1246-7820
    DOI 10.1016/j.tracli.2023.03.001
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    Zs.MO 308: Show issues

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  7. Article ; Online: A Novel Approach to Predicting Early Pregnancy Outcomes Dynamically in a Prospective Cohort Using Repeated Ultrasound and Serum Biomarkers.

    Petersen, Jesper Friis / Friis-Hansen, Lennart Jan / Bryndorf, Thue / Jensen, Andreas Kryger / Andersen, Anders Nyboe / Løkkegaard, Ellen

    Reproductive sciences (Thousand Oaks, Calif.)

    2023  Volume 30, Issue 12, Page(s) 3597–3609

    Abstract: This study aimed to develop a dynamic model for predicting outcome during the first trimester of pregnancy using baseline demographic data and serially collected blood samples and transvaginal sonographies. A prospective cohort of 203 unselected women ... ...

    Abstract This study aimed to develop a dynamic model for predicting outcome during the first trimester of pregnancy using baseline demographic data and serially collected blood samples and transvaginal sonographies. A prospective cohort of 203 unselected women with an assumed healthy pregnancy of < 8 weeks' gestation was followed fortnightly from 4-14 weeks' gestation until either miscarriage or confirmed first trimester viability. The main outcome was development of a model to predict outcome from gestational age-dependent hazard ratios using both baseline and updated serial data from each visit. Secondary outcomes were descriptions of risk factors for miscarriage. The results showed that 18% of the women experienced miscarriages. A fetal heart rate detected before 8 weeks' gestation indicated a 90% (95% CI 85-95%) chance of subsequent delivery. Maternal age (≥ 35 years), insufficient crown-rump-length (CRL) and mean gestational sac diameter (MSD) development, and presence of bleeding increased the risk of miscarriage. Serum biomarkers, including hCG, progesterone, and estradiol, were found to impact the risk of miscarriage with estradiol as the most important. The best model to predict miscarriage was a combination of maternal age, vaginal bleeding, CRL, and hCG. The second-best model was the sonography-absent model of maternal age, bleeding, hCG, and estradiol. This study suggests that combining maternal age, and evolving data from hCG, estradiol, CRL, and bleeding could be used to predict fetal outcome during the first trimester of pregnancy.Trial registration ClinicalTrials.gov identifier: NCT02761772.
    MeSH term(s) Pregnancy ; Humans ; Female ; Adult ; Pregnancy Outcome ; Abortion, Spontaneous/etiology ; Prospective Studies ; Ultrasonography, Prenatal/methods ; Pregnancy Trimester, First ; Biomarkers ; Estradiol
    Chemical Substances Biomarkers ; Estradiol (4TI98Z838E)
    Language English
    Publishing date 2023-08-28
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2276411-2
    ISSN 1933-7205 ; 1933-7191
    ISSN (online) 1933-7205
    ISSN 1933-7191
    DOI 10.1007/s43032-023-01323-8
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  8. Article ; Online: The HemoScreen hematology point-of-care device is suitable for rapid evaluation of acute leukemia patients.

    Kristian Kur, Dår / Thøgersen, Danny / Kjeldsen, Lars / Friis-Hansen, Lennart

    International journal of laboratory hematology

    2020  Volume 43, Issue 1, Page(s) 52–60

    Abstract: Background: Hematological patients, receiving intensive chemotherapy (predominantly acute leukemia patients), have repeated postchemotherapy periods with severe bone marrow suppression. As a result, these patients require regular monitoring of the ... ...

    Abstract Background: Hematological patients, receiving intensive chemotherapy (predominantly acute leukemia patients), have repeated postchemotherapy periods with severe bone marrow suppression. As a result, these patients require regular monitoring of the complete blood counts (CBC) for optimal patient care. To reduce the strain on the patient, there is a need for a point-of-care (POC) hematology device that provides rapid and reliable results both in general and in cytopenic samples and is suitable for outpatient clinics. We evaluated the HemoScreen device for the most used CBC parameter both overall and at the lower range.
    Methods: The HemoScreen was compared with the Sysmex XN-9000 in 206 routine venous samples and 79 capillary bedside samples focusing on white blood cells (WBC), absolute neutrophil count (ANC), red blood cells (RBC), PLT and HGB.
    Results: The HemoScreen was less precise compared to the acceptance criteria set for larger and more advanced hematology instrument with a CV% 3.0-3.7 for WBCs, 3.6-8.4 for ANCs, 1.1-1.5 for RBCs, 2.5-4.4 for PLTs, and 1.7-2.3 for HGB. Correlation coefficient for all five parameters for the entire range was r >.95 and r >.90 at lower range for venous and capillary samples. Bias limits were within the CTCAE acceptance limits.
    Conclusions: The HemoScreen provides rapid and accurate test results, for evaluation of WBC, PLT, and HGB, as well as at low concentrations for guiding transfusions and postchemotherapy treatment. The device is easy to operate and can measure both venous and capillary samples. Therefore, the HemoScreen is well suited for smaller outpatient clinics and potentially home use.
    MeSH term(s) Acute Disease ; Blood Cell Count/instrumentation ; Humans ; Leukemia/blood ; Point-of-Care Systems
    Keywords covid19
    Language English
    Publishing date 2020-09-07
    Publishing country England
    Document type Evaluation Study ; Journal Article
    ZDB-ID 2268590-X
    ISSN 1751-553X ; 1751-5521 ; 0141-9854
    ISSN (online) 1751-553X
    ISSN 1751-5521 ; 0141-9854
    DOI 10.1111/ijlh.13330
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  9. Article ; Online: Serological response following vaccination with BNT162b2 mRNA in patients with chronic lymphocytic leukemia.

    da Cunha-Bang, Caspar / Kirkby, Nikolai S / Friis-Hansen, Lennart / Niemann, Carsten U

    Leukemia & lymphoma

    2021  Volume 63, Issue 2, Page(s) 503–505

    MeSH term(s) Antibodies, Viral ; BNT162 Vaccine ; Humans ; Leukemia, Lymphocytic, Chronic, B-Cell/genetics ; RNA, Messenger/genetics ; Vaccination
    Chemical Substances Antibodies, Viral ; RNA, Messenger ; BNT162 Vaccine (N38TVC63NU)
    Language English
    Publishing date 2021-11-02
    Publishing country United States
    Document type Letter ; Research Support, Non-U.S. Gov't
    ZDB-ID 1042374-6
    ISSN 1029-2403 ; 1042-8194
    ISSN (online) 1029-2403
    ISSN 1042-8194
    DOI 10.1080/10428194.2021.1973673
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  10. Article ; Online: Evaluation of the HemoCue WBC DIFF in leukopenic patient samples.

    Kur, Dår K / Agersnap, Niels / Holländer, Niels Henrik / Pedersen, Ole B Vesterager / Friis-Hansen, Lennart

    International journal of laboratory hematology

    2020  Volume 42, Issue 3, Page(s) 256–262

    Abstract: Background: White blood cell (WBC) counts are used to monitor bone marrow function and to screen for infections. The HemoCue WBC DIFF Point-Of-Care (POC) instrument classifies WBCs through cell image recognition. To evaluate its suitability for ... ...

    Abstract Background: White blood cell (WBC) counts are used to monitor bone marrow function and to screen for infections. The HemoCue WBC DIFF Point-Of-Care (POC) instrument classifies WBCs through cell image recognition. To evaluate its suitability for monitoring cancer patients, we examined its performance in samples from patient with leukopenia and in samples containing nRBC.
    Methods: Sysmex samples with WBCs 0.05-3.40 × 10
    Results: The counting variation was primarily the source of statistical error in the lower counts with an imprecision between 3.8-9.2% for total WBC (0.56-2.29 ×10
    Conclusions: The HemoCue WBC DIFF provided reliable and accurate counts of total WBC, neutrophil, and lymphocyte in leukopenic samples. Until POC instruments that can perform an accurate complete blood count are available, the HemoCue WBC DIFF can be used to assist physicians in making decisions in situations of postchemotherapy leukopenia and neutropenia.
    MeSH term(s) Adult ; Humans ; Leukocyte Count/instrumentation ; Leukopenia/blood ; Male ; Neoplasms/blood ; Point-of-Care Testing
    Language English
    Publishing date 2020-02-10
    Publishing country England
    Document type Evaluation Study ; Journal Article
    ZDB-ID 2268590-X
    ISSN 1751-553X ; 1751-5521 ; 0141-9854
    ISSN (online) 1751-553X
    ISSN 1751-5521 ; 0141-9854
    DOI 10.1111/ijlh.13158
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