Article ; Online: Cell-based gene therapy for repair of critical size defects in the rat fibula.
Journal of cellular biochemistry
2011 Volume 112, Issue 6, Page(s) 1563–1571
Abstract: More than a decade has passed since the first experiments using adenovirus-transduced cells expressing bone morphogenetic protein 2 were performed for the synthesis of bone. Since this time, the field of bone gene therapy has tackled many issues ... ...
| Abstract | More than a decade has passed since the first experiments using adenovirus-transduced cells expressing bone morphogenetic protein 2 were performed for the synthesis of bone. Since this time, the field of bone gene therapy has tackled many issues surrounding safety and efficacy of this type of strategy. We present studies examining the parameters of the timing of bone healing, and remodeling when heterotopic ossification (HO) is used for bone fracture repair using an adenovirus gene therapy approach. We use a rat fibula defect, which surprisingly does not heal even when a simple fracture is introduced. In this model, the bone quickly resorbs most likely due to the non-weight bearing nature of this bone in rodents. Using our gene therapy system robust HO can be introduced at the targeted location of the defect resulting in bone repair. The HO and resultant bone healing appeared to be dose dependent, based on the number of AdBMP2-transduced cells delivered. Interestingly, the HO undergoes substantial remodeling, and assumes the size and shape of the missing segment of bone. However, in some instances we observed some additional bone associated with the repair, signifying that perhaps the forces on the newly forming bone are inadequate to dictate shape. In all cases, the HO appeared to fuse into the adjacent long bone. The data collectively indicates that the use of BMP2 gene therapy strategies may vary depending on the location and nature of the defect. Therefore, additional parameters should be considered when implementing such strategies. |
|||||
|---|---|---|---|---|---|---|
| MeSH term(s) | Adenoviridae/genetics ; Animals ; Bone Morphogenetic Protein 2/genetics ; Bone Morphogenetic Protein 2/metabolism ; Bone and Bones/abnormalities ; Cell Line ; Cell- and Tissue-Based Therapy/methods ; Fibula/abnormalities ; Genetic Therapy/methods ; Humans ; Mice ; Osteogenesis/physiology ; Rats ; Wound Healing/physiology | |||||
| Chemical Substances | Bone Morphogenetic Protein 2 | |||||
| Language | English | |||||
| Publishing date | 2011-02-23 | |||||
| Publishing country | United States | |||||
| Document type | Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. | |||||
| ZDB-ID | 392402-6 | |||||
| ISSN | 1097-4644 ; 0730-2312 | |||||
| ISSN (online) | 1097-4644 | |||||
| ISSN | 0730-2312 | |||||
| DOI | 10.1002/jcb.23068 | |||||
| Shelf mark |
|
|||||
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
Full text online
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Zs.A 1114: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.