Article: Production of two types of phytase from Aspergillus oryzae during industrial koji making.
Journal of bioscience and bioengineering
2005 Volume 95, Issue 5, Page(s) 460–465
Abstract: In our previous study, it was determined that phytase produced by Aspergillus oryzae plays an important role in supplying phosphate to yeast in the process of making sake. During koji making, two types of phytase (Phy-I and Phy-II) are produced. The ... ...
| Abstract | In our previous study, it was determined that phytase produced by Aspergillus oryzae plays an important role in supplying phosphate to yeast in the process of making sake. During koji making, two types of phytase (Phy-I and Phy-II) are produced. The purified phytases have high thermal and pH stability, in comparison to phytase purified from a submerged culture (ACP-II). In the present study, Phy-I and Phy-II retained their activities for 45 h. The NH2-terminal sequence of Phy-1, which is eight amino acids in length, was identical to that of ACP-II, but the molecular weights of these two forms, as estimated by SDS-PAGE, were quite different from each other (Phy-I, 120 kDa; ACP-II, 58 kDa). From the NH2-terminal amino acid sequence analysis of the predominant phytase (Phy-II), a molecular weight of 116 kDa was expected to reflect a new type of phytase produced only in koji culture. The substrate specificity of Phy-II was sufficiently broad that it hydrolyzed not only phytic acid and p-nitro phenyl phosphate, but also glucose 6-phosphate and glycerol 1-phosphate. In the process of making koji, Phy-I was produced at an early stage, followed by Phy-II; with both phytases being thought to function to hydrolyze phytic acid cooperatively. |
|---|---|
| Language | English |
| Publishing date | 2005-10-03 |
| Publishing country | Japan |
| Document type | Journal Article |
| ZDB-ID | 1465387-4 |
| ISSN | 1347-4421 ; 1389-1723 |
| ISSN (online) | 1347-4421 |
| ISSN | 1389-1723 |
| DOI | 10.1016/s1389-1723(03)80045-2 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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