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Article ; Online: Exploring the potential of phenolic and antioxidant compounds in new Rosaceae fruits.

Razola-Díaz, María Del Carmen / Aznar-Ramos, María José / Benítez, Guillermo / Gómez-Caravaca, Ana María / Verardo, Vito

Journal of the science of food and agriculture

2024 Volume 104, Issue 6, Page(s) 3705–3718

Abstract: Background: Rosaceae fruits have been used in traditional medicine for the prevention and treatment of diseases. However, these fruits have not extensively been studied regarding their phenolic composition. Thus, this research focuses on the ... ...

Abstract	Background: Rosaceae fruits have been used in traditional medicine for the prevention and treatment of diseases. However, these fruits have not extensively been studied regarding their phenolic composition. Thus, this research focuses on the determination of phenolic compounds by high-performance liquid chromatography electrospray ionization time-of-flight mass spectrometry, flavan-3-ols by high-performance liquid chromatography with fluorescence detection, and the antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and ferric reducing antioxidant power of the fruits of five species of genera Crataegus and Sorbus (Rosaceae). Results: We found a total of 71 phenolic compounds from which 30 were identified in these berries for the first time. Crataegus monogyna and Crataegus laciniata revealed higher total phenolic and flavan-3-ol contents than the other species and the highest antioxidant activities. Conclusions: Therefore, the fruits evaluated have demonstrated to be important sources of bioactive compounds with huge potential for being used in nutraceutical or food scopes. Additional studies could be needed to evaluate the influence of the different production areas on the phenolic content. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
MeSH term(s)	Antioxidants/chemistry ; Rosaceae/chemistry ; Fruit/chemistry ; Phenols/chemistry ; Chromatography, High Pressure Liquid/methods
Chemical Substances	Antioxidants ; Phenols
Language	English
Publishing date	2024-01-20
Publishing country	England
Document type	Journal Article
ZDB-ID	184116-6
ISSN	1097-0010 ; 0022-5142
ISSN (online)	1097-0010
ISSN	0022-5142
DOI	10.1002/jsfa.13255
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Article ; Online: Evaluation of saponin and phenolic profile of quinoa seeds after fungal fermentation

Starzyńska-Janiszewska, Anna / Stodolak, Bożena / Duliński, Robert / Fernández-Fernández, Carmen / Martín-García, Beatriz / Gómez-Caravaca, Ana María

Journal of Cereal Science. 2023 Mar. 01, p.103656-

2023 , Page(s) 103656–

Abstract: Solid-state fermentation can be an effective method of modelling the bioactive potential of plant materials, but the result is substrate and strain dependent. In this study, edible fungi traditionally used in Asian food fermentations (tempe moulds - ... ...

Abstract	Solid-state fermentation can be an effective method of modelling the bioactive potential of plant materials, but the result is substrate and strain dependent. In this study, edible fungi traditionally used in Asian food fermentations (tempe moulds - Rhizopus oligosporus, R. oryzae and R. chinensis, koji mould - Aspergillus oryzae, oncom mould - Neurospora intermedia, and Mucor indicus) were grown on high-saponin quinoa seeds for 4 days at 30 °C. The sapogenin profile in the substrate was not changed after the fermentation. Whereas, the level and profile of phenolic compounds, as well as the antioxidant activity of the seeds, were diversified. The fermentation with R. chinensis, R. oryzae and A. oryzae caused an increase in soluble quercetin derivatives. Moreover, the concentration of bound phenolics was higher in the quinoa seeds fermented with most Rhizopus strains. The majority of the moulds were also efficient in the decomposition of phytate. Among the strains tested, the most promising results were obtained in the case of R. chinensis and R. oryzae. Further research is required to study their potential for the purpose of the fermentation of cereal and pseudo-cereal substrates.
Keywords	Aspergillus oryzae ; Mucor ; Neurospora ; Rhizopus microsporus var. oligosporus ; antioxidant activity ; fungi ; koji ; phenolic compounds ; phytic acid ; pseudocereals ; quercetin ; saponins ; solid state fermentation ; tempeh ; Quinoa ; Phenolics ; Fermentation
Language	English
Dates of publication	2023-0301
Publishing place	Elsevier Ltd
Document type	Article ; Online
Note	Pre-press version
ZDB-ID	408468-8
ISSN	0733-5210
ISSN	0733-5210
DOI	10.1016/j.jcs.2023.103656
Database	NAL-Catalogue (AGRICOLA)

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Article: The Establishment of Ultrasonic-Assisted Extraction for the Recovery of Phenolic Compounds and Evaluation of Their Antioxidant Activity from Morus alba Leaves

Martín-García, Beatriz / Aznar-Ramos, María José / Verardo, Vito / Gómez-Caravaca, Ana María

Foods. 2022 Jan. 24, v. 11, no. 3

2022

Abstract: Phenolic compounds of Morus alba leaves are bioactive compounds with beneficial properties for human health. Therefore, in this study, an optimization of ultrasonic assisted extraction by Box–Behnken design was used for the first time to optimize factors ...

Abstract	Phenolic compounds of Morus alba leaves are bioactive compounds with beneficial properties for human health. Therefore, in this study, an optimization of ultrasonic assisted extraction by Box–Behnken design was used for the first time to optimize factors such as the percentage of ethanol, ratio solvent/sample (v/w) and extraction time to reach the highest phenolic compound amounts (evaluated by HPLC-MS) while also evaluating in vitro antioxidant activity using DPPH, ABTS and FRAP assays. The optimal extraction conditions were 40% ethanol, 1/400 (w/v) and 35 min. Applying these optimal conditions, which were identified and quantified by HPLC-MS, resulted in the extraction of 21 phenolic compounds. According to these results, the main phenolic compounds in Morus alba leaves are the phenolic glycoside and phenolic acid named protocatechuic acid-glucoside and caffeoylquinic. In addition, Morus alba leaf extract contains flavonols such quercetin-3-O-6-acetylglucoside and rutin, which represent more than 7% of its total phenolic content.
Keywords	Morus alba ; antioxidant activity ; ethanol ; experimental design ; glycosides ; human health ; leaf extracts ; phenolic acids ; rutin ; solvents ; ultrasonics
Language	English
Dates of publication	2022-0124
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods11030314
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Article: Development of an Effective Sonotrode Based Extraction Technique for the Recovery of Phenolic Compounds with Antioxidant Activities in Cherimoya Leaves

Martín-García, Beatriz / Aznar-Ramos, María José / Verardo, Vito / Gómez-Caravaca, Ana María

Plants. 2022 Aug. 04, v. 11, no. 15

2022

Abstract: The leaves of Annona cherimola Mill (cherimoya) are a potential source of phenolic compounds that have been shown to have beneficial properties. Therefore, this study focuses on establishing an ultrasonic-assisted extraction of phenolic compounds in ... ...

Abstract	The leaves of Annona cherimola Mill (cherimoya) are a potential source of phenolic compounds that have been shown to have beneficial properties. Therefore, this study focuses on establishing an ultrasonic-assisted extraction of phenolic compounds in cherimoya leaves using a sonotrode. For that purpose, a Box-Behnken design based on a response surface methodology (RSM) was used to optimize factors, such as amplitude, extraction time and solvent composition to obtain the maximum content of phenolic compounds by HPLC-MS and the maximum in-vitro antioxidant activity by DPPH, ABTS and FRAP assays in ‘Fino de Jete’ cherimoya leaves. The optimal conditions were 70% amplitude, 10 min and 40:60 ethanol/water (EtOH/H₂O) (v/v). The results obtained under these optimum conditions by using a sonotrode were compared with those from an ultrasonic bath; briefly, recovery of phenolic compounds by sonotrode was 2.3 times higher than a bath. Therefore, these optimal conditions were applied to different varieties ‘Campas’, ‘Fino de Jete’ and ‘Negrito Joven’ harvested in the Tropical Coast of Granada (Spain). A total of 39 phenolic compounds were determined in these cherimoya leaf extracts, 24 phenolic compounds by HPLC-MS and 15 proanthocianidins by HPLC-FLD. 5-p-coumaroylquinic acid, lathyroside-7-O-α-l-rhamnopyranoside and quercetin hexose acetate were first identified in cherimoya leaves. The most concentrated phenolic compounds were the flavonoids, such as rutin and quercetin hexoside and proanthocyanidins including monomers. Almost no significant differences in the phenolic content in these cultivars were found (11–13 mg/g d.w. for phenolic compounds and 11–20 mg/g d.w. for proanthocyanidins). In addition, sonotrode ultrasonic-assisted extraction has been shown to be an efficient extraction technique in the phenolic recovery from cherimoya leaves that could be implemented on an industrial scale.
Keywords	Annona cherimola ; acetates ; antioxidant activity ; antioxidants ; cherimoyas ; coasts ; cultivars ; ethanol ; experimental design ; leaves ; proanthocyanidins ; quercetin ; response surface methodology ; rutin ; solvents ; ultrasonics ; Spain
Language	English
Dates of publication	2022-0804
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants11152034
Database	NAL-Catalogue (AGRICOLA)

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Article: Bioactive Components in Fermented Foods and Food By-Products.

Verardo, Vito / Gómez-Caravaca, Ana Maria / Tabanelli, Giulia

Foods (Basel, Switzerland)

2020 Volume 9, Issue 2

Abstract: Food fermentation is one of the most ancient processes of food production that has historically been used to extend food shelf life and to enhance its organoleptic properties [ ... ]. ...

Abstract	Food fermentation is one of the most ancient processes of food production that has historically been used to extend food shelf life and to enhance its organoleptic properties [...].
Language	English
Publishing date	2020-02-05
Publishing country	Switzerland
Document type	Editorial
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods9020153
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Article: The Establishment of Ultrasonic-Assisted Extraction for the Recovery of Phenolic Compounds and Evaluation of Their Antioxidant Activity from

Martín-García, Beatriz / Aznar-Ramos, María José / Verardo, Vito / Gómez-Caravaca, Ana María

Foods (Basel, Switzerland)

2022 Volume 11, Issue 3

Abstract: Phenolic compounds ... ...

Abstract	Phenolic compounds of
Language	English
Publishing date	2022-01-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods11030314
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Article ; Online: Assessment of Lipid Quality in Commercial Omega-3 Supplements Sold in the French Market.

Pasini, Federica / Gómez-Caravaca, Ana Maria / Blasco, Thierry / Cvejić, Jelena / Caboni, Maria Fiorenza / Verardo, Vito

Biomolecules

2022 Volume 12, Issue 10

Abstract: Supplementation of omega-3 fatty acids is considered a valuable strategy to supply the low intake of these fatty acids. Thus, the safety of the supplements is an important milestone. Because of that, we analyzed 20 unflavored supplements sold in the ... ...

Abstract	Supplementation of omega-3 fatty acids is considered a valuable strategy to supply the low intake of these fatty acids. Thus, the safety of the supplements is an important milestone. Because of that, we analyzed 20 unflavored supplements sold in the French market for fatty acid and triglyceride composition, for EPA and DHA, and for tocol content, as well as for oxidative status. This study found that only 2.5% of the supplements did not meet their label claims for omega-3 content. TAG analysis showed high variability among the triglyceride distribution, and the same trend was also noticed for the tocol content; in fact, a high variability of the distribution of the six tocols (four tocopherols and two tocotrienols) was found among the samples. Of the tested products, all of them complied with peroxide value, p-anisidine value, and Totox values established by the Global Organization for EPA and DHA Omega-3s (GOED) and were not oxidized.
MeSH term(s)	Eicosapentaenoic Acid/analysis ; Docosahexaenoic Acids/analysis ; Tocotrienols ; Fatty Acids, Omega-3/analysis ; Dietary Supplements/analysis ; Fatty Acids ; Triglycerides ; Tocopherols ; Peroxides ; Fish Oils
Chemical Substances	Eicosapentaenoic Acid (AAN7QOV9EA) ; Docosahexaenoic Acids (25167-62-8) ; Tocotrienols ; Fatty Acids, Omega-3 ; Fatty Acids ; Triglycerides ; Tocopherols (R0ZB2556P8) ; Peroxides ; Fish Oils
Language	English
Publishing date	2022-09-23
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2701262-1
ISSN	2218-273X ; 2218-273X
ISSN (online)	2218-273X
ISSN	2218-273X
DOI	10.3390/biom12101361
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Article: Development of an Effective Sonotrode Based Extraction Technique for the Recovery of Phenolic Compounds with Antioxidant Activities in Cherimoya Leaves.

Martín-García, Beatriz / Aznar-Ramos, María José / Verardo, Vito / Gómez-Caravaca, Ana María

Plants (Basel, Switzerland)

2022 Volume 11, Issue 15

Abstract: The leaves ... ...

Abstract	The leaves of
Language	English
Publishing date	2022-08-04
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants11152034
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Article: Mathematical Modelling of Convective Drying of Orange By-Product and Its Influence on Phenolic Compounds and Ascorbic Acid Content, and Its Antioxidant Activity.

Razola-Díaz, María Del Carmen / Verardo, Vito / Gómez-Caravaca, Ana María / García-Villanova, Belén / Guerra-Hernández, Eduardo Jesús

Foods (Basel, Switzerland)

2023 Volume 12, Issue 3

Abstract: Orange peel is one of the main by-products from juice processing, and is considered as a promising source of phenolic compounds with anti-carcinogenic, anti-inflammatory, anti-microbial and antioxidant properties. The drying is an essential step to ... ...

Abstract	Orange peel is one of the main by-products from juice processing, and is considered as a promising source of phenolic compounds with anti-carcinogenic, anti-inflammatory, anti-microbial and antioxidant properties. The drying is an essential step to ensure the storage of this by-product at an industrial level, in order to use it as a functional ingredient or as a nutraceutical. Thus, this research focuses on the evaluation of the effect of the convective air-drying process in orange by-products at three different temperatures (40, 60 and 80 °C) and air flows (0, 0.8 and 1.6 m/s) on the phenolic content (measured by HPLC-MS), the antioxidant activity (measured by DPPH, ABTS and FRAP), and the vitamin C content (measured by HPLC-UV/VIS). Moreover, the mathematical modelling of its drying kinetics was carried out to examine the orange by-product behavior. Among the tested mathematical models, the Page model reported the highest fit and the best drying conditions, which showed the lowest reductions were at 60 °C with an air flow of 1.6 m/s and taking 315 min.
Language	English
Publishing date	2023-01-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods12030500
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Article: Response Surface Methodology for the Optimization of Flavan-3-ols Extraction from Avocado By-Products via Sonotrode Ultrasound-Assisted Extraction.

Razola-Díaz, María Del Carmen / Verardo, Vito / Guerra-Hernández, Eduardo Jesús / García-Villanova Ruiz, Belén / Gómez-Caravaca, Ana María

Antioxidants (Basel, Switzerland)

2023 Volume 12, Issue 7

Abstract: Avocado peel and seed are the main by-products of avocado processing and are considered as promising sources of phenolic compounds with biological activities. Thus, this research focuses on the establishment, for the first time, of ultrasound-assisted ... ...

Abstract	Avocado peel and seed are the main by-products of avocado processing and are considered as promising sources of phenolic compounds with biological activities. Thus, this research focuses on the establishment, for the first time, of ultrasound-assisted extraction of flavan-3-ols with high antioxidant activity from avocado peel and seed using a sonotrode. Indeed, 2 Box-Behnken designs were performed for 15 experiments, with each design having three independent factors (ratio ethanol/water (
Language	English
Publishing date	2023-07-11
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704216-9
ISSN	2076-3921
ISSN	2076-3921
DOI	10.3390/antiox12071409
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