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  1. Article: A rapid and simple procedure for the isolation and cultivation of fibroblast-like cells from medaka and zebrafish embryos and fin clip biopsies

    Beedgen, Lars / Hüllen, Andreas / Gücüm, Sevinç / Thumberger, Thomas / Wittbrodt, Jochen / Thiel, Christian

    Laboratory animals. 2022 June, v. 56, no. 3

    2022  

    Abstract: In many human diseases, the molecular pathophysiological mechanisms are not understood, which makes the development and testing of new therapeutic approaches difficult. The generation and characterization of animal models such as mice, rats, fruit flies, ...

    Abstract In many human diseases, the molecular pathophysiological mechanisms are not understood, which makes the development and testing of new therapeutic approaches difficult. The generation and characterization of animal models such as mice, rats, fruit flies, worms or fish offers the possibility for in detail studies of a disease’s development, its course and potential therapies in an organismal context, which considerably minimizes the risk of therapeutic side effects for patients. Nevertheless, due to the high numbers of experimental animals used in research worldwide, attempts to develop alternative test systems will help in reducing their count. In this regard, the cell culture system displays a suitable option due to its potential of delivering nearly unlimited material and the good opportunities for high-throughput studies such as drug testing. Here, we describe a quick and simple method to isolate and cultivate vital fibroblast-like cells from embryos and adults of two popular teleost model organisms, the Japanese rice fish medaka (Oryzias latipes) and the zebrafish (Danio rerio).
    Keywords Danio rerio ; Oryzias latipes ; cell culture ; drugs ; fish ; fruits ; humans ; laboratories ; risk
    Language English
    Dates of publication 2022-06
    Size p. 270-278.
    Publishing place SAGE Publications
    Document type Article
    ZDB-ID 391008-8
    ISSN 1758-1117 ; 0023-6772
    ISSN (online) 1758-1117
    ISSN 0023-6772
    DOI 10.1177/00236772211045483
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

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  2. Book ; Online ; Thesis: Modeling hypo-N-glycosylation in medaka, Oryzias latipes, to decipher mechanisms of Congenital Disorders of Glycosylation

    Gücüm, Sevinç [Verfasser] / Lohmann, Ingrid [Akademischer Betreuer]

    2021  

    Author's details Sevinc Gücüm ; Betreuer: Ingrid Lohmann
    Keywords Biowissenschaften, Biologie ; Life Science, Biology
    Subject code sg570
    Language English
    Publisher Universitätsbibliothek Heidelberg
    Publishing place Heidelberg
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  3. Article ; Online: A rapid and simple procedure for the isolation and cultivation of fibroblast-like cells from medaka and zebrafish embryos and fin clip biopsies.

    Beedgen, Lars / Hüllen, Andreas / Gücüm, Sevinç / Thumberger, Thomas / Wittbrodt, Jochen / Thiel, Christian

    Laboratory animals

    2021  Volume 56, Issue 3, Page(s) 270–278

    Abstract: In many human diseases, the molecular pathophysiological mechanisms are not understood, which makes the development and testing of new therapeutic approaches difficult. The generation and characterization of animal models such as mice, rats, fruit flies, ...

    Abstract In many human diseases, the molecular pathophysiological mechanisms are not understood, which makes the development and testing of new therapeutic approaches difficult. The generation and characterization of animal models such as mice, rats, fruit flies, worms or fish offers the possibility for in detail studies of a disease's development, its course and potential therapies in an organismal context, which considerably minimizes the risk of therapeutic side effects for patients. Nevertheless, due to the high numbers of experimental animals used in research worldwide, attempts to develop alternative test systems will help in reducing their count. In this regard, the cell culture system displays a suitable option due to its potential of delivering nearly unlimited material and the good opportunities for high-throughput studies such as drug testing. Here, we describe a quick and simple method to isolate and cultivate vital fibroblast-like cells from embryos and adults of two popular teleost model organisms, the Japanese rice fish medaka (
    MeSH term(s) Animals ; Biopsy ; Fibroblasts ; Humans ; Mice ; Oryzias ; Rats ; Surgical Instruments ; Zebrafish
    Language English
    Publishing date 2021-09-22
    Publishing country England
    Document type Journal Article
    ZDB-ID 391008-8
    ISSN 1758-1117 ; 0023-6772
    ISSN (online) 1758-1117
    ISSN 0023-6772
    DOI 10.1177/00236772211045483
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  4. Article ; Online: A patient-based medaka alg2 mutant as a model for hypo-N-glycosylation.

    Gücüm, Sevinç / Sakson, Roman / Hoffmann, Marcus / Grote, Valerian / Becker, Clara / Pakari, Kaisa / Beedgen, Lars / Thiel, Christian / Rapp, Erdmann / Ruppert, Thomas / Thumberger, Thomas / Wittbrodt, Joachim

    Development (Cambridge, England)

    2021  Volume 148, Issue 11

    Abstract: Defects in the evolutionarily conserved protein-glycosylation machinery during embryonic development are often fatal. Consequently, congenital disorders of glycosylation (CDG) in human are rare. We modelled a putative hypomorphic mutation described in an ...

    Abstract Defects in the evolutionarily conserved protein-glycosylation machinery during embryonic development are often fatal. Consequently, congenital disorders of glycosylation (CDG) in human are rare. We modelled a putative hypomorphic mutation described in an alpha-1,3/1,6-mannosyltransferase (ALG2) index patient (ALG2-CDG) to address the developmental consequences in the teleost medaka (Oryzias latipes). We observed specific, multisystemic, late-onset phenotypes, closely resembling the patient's syndrome, prominently in the facial skeleton and in neuronal tissue. Molecularly, we detected reduced levels of N-glycans in medaka and in the patient's fibroblasts. This hypo-N-glycosylation prominently affected protein abundance. Proteins of the basic glycosylation and glycoprotein-processing machinery were over-represented in a compensatory response, highlighting the regulatory topology of the network. Proteins of the retinal phototransduction machinery, conversely, were massively under-represented in the alg2 model. These deficiencies relate to a specific failure to maintain rod photoreceptors, resulting in retinitis pigmentosa characterized by the progressive loss of these photoreceptors. Our work has explored only the tip of the iceberg of N-glycosylation-sensitive proteins, the function of which specifically impacts on cells, tissues and organs. Taking advantage of the well-described human mutation has allowed the complex interplay of N-glycosylated proteins and their contribution to development and disease to be addressed.
    MeSH term(s) Animals ; Congenital Disorders of Glycosylation/genetics ; Congenital Disorders of Glycosylation/metabolism ; Disease Models, Animal ; Fibroblasts/metabolism ; Glycoproteins/genetics ; Glycoproteins/metabolism ; Glycosylation ; Humans ; Mannosyltransferases/genetics ; Mannosyltransferases/metabolism ; Mutation ; Oryzias/genetics ; Oryzias/metabolism ; Phenotype ; Polysaccharides ; Retinitis Pigmentosa
    Chemical Substances Glycoproteins ; Polysaccharides ; Mannosyltransferases (EC 2.4.1.-)
    Language English
    Publishing date 2021-06-09
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 90607-4
    ISSN 1477-9129 ; 0950-1991
    ISSN (online) 1477-9129
    ISSN 0950-1991
    DOI 10.1242/dev.199385
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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