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Article ; Online: Influence of native ureolytic microbial community on biocementation potential of Sporosarcina pasteurii

Raja Murugan / G. K. Suraishkumar / Abhijit Mukherjee / Navdeep K. Dhami

Scientific Reports, Vol 11, Iss 1, Pp 1-

2021 Volume 12

Abstract: Abstract Microbially induced calcium carbonate precipitation (MICP)/Biocementation has emerged as a promising technique for soil engineering applications. There are chiefly two methods by which MICP is applied for field applications including ... ...

Abstract	Abstract Microbially induced calcium carbonate precipitation (MICP)/Biocementation has emerged as a promising technique for soil engineering applications. There are chiefly two methods by which MICP is applied for field applications including biostimulation and bioaugmentation. Although bioaugmentation strategy using efficient ureolytic biocementing culture of Sporosarcina pasteurii is widely practiced, the impact of native ureolytic microbial communities (NUMC) on CaCO3 mineralisation via S. pasteurii has not been explored. In this paper, we investigated the effect of different concentrations of NUMC on MICP kinetics and biomineral properties in the presence and absence of S. pasteurii. Kinetic analysis showed that the biocementation potential of S. pasteurii is sixfold higher than NUMC and is not significantly impacted even when the concentration of the NUMC is eight times higher. Micrographic results revealed a quick rate of CaCO3 precipitation by S. pasteurii leading to generation of smaller CaCO3 crystals (5–40 µm), while slow rate of CaCO3 precipitation by NUMC led to creation of larger CaCO3 crystals (35–100 µm). Mineralogical results showed the predominance of calcite phase in both sets. The outcome of current study is crucial for tailor-made applications of MICP.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2021-10-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Insights into the influence of cell concentration in design and development of microbially induced calcium carbonate precipitation (MICP) process.

Raja Murugan / G K Suraishkumar / Abhijit Mukherjee / Navdeep K Dhami

PLoS ONE, Vol 16, Iss 7, p e

2021 Volume 0254536

Abstract: Microbially induced calcium carbonate precipitation (MICP) process utilising the biogeochemical reactions for low energy cementation has recently emerged as a potential technology for numerous engineering applications. The design and development of an ... ...

Abstract	Microbially induced calcium carbonate precipitation (MICP) process utilising the biogeochemical reactions for low energy cementation has recently emerged as a potential technology for numerous engineering applications. The design and development of an efficient MICP process depends upon several physicochemical and biological variables; amongst which the initial bacterial cell concentration is a major factor. The goal of this study is to assess the impact of initial bacterial cell concentration on ureolysis and carbonate precipitation kinetics along with its influence on the calcium carbonate crystal properties; as all these factors determine the efficacy of this process for specific engineering applications. We have also investigated the role of subsequent cell recharge in calcium carbonate precipitation kinetics for the first time. Experimental results showed that the kinetics of ureolysis and calcium carbonate precipitation are well-fitted by an exponential logistic equation for cell concentrations between optical density range of 0.1 OD to 0.4 OD. This equation is highly applicable for designing the optimal processes for microbially cemented soil stabilization applications using native or augmented bacterial cultures. Multiple recharge kinetics study revealed that the addition of fresh bacterial cells is an essential step to keep the fast rate of precipitation, as desirable in certain applications. Our results of calcium carbonate crystal morphology and mineralogy via scanning electron micrography, energy dispersive X-ray spectroscopy and X-ray diffraction analysis exhibited a notable impact of cell number and extracellular urease concentration on the properties of carbonate crystals. Lower cell numbers led to formation of larger crystals compared to high cell numbers and these crystals transform from vaterite phase to the calcite phase over time. This study has demonstrated the significance of kinetic models for designing large-scale MICP applications.
Keywords	Medicine ; R ; Science ; Q
Subject code	500
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Metabolic modeling of host–microbe interactions for therapeutics in colorectal cancer

Prerna Bhalla / Raghunathan Rengaswamy / Devarajan Karunagaran / G. K. Suraishkumar / Swagatika Sahoo

npj Systems Biology and Applications, Vol 8, Iss 1, Pp 1-

2022 Volume 11

Abstract: Abstract The onset of colorectal cancer (CRC) is often attributed to gut bacterial dysbiosis, and thus gut microbiota are highly relevant in devising treatment strategies. Certain gut microbes, like Enterococcus spp., exhibit remarkable anti-neoplastic ... ...

Abstract	Abstract The onset of colorectal cancer (CRC) is often attributed to gut bacterial dysbiosis, and thus gut microbiota are highly relevant in devising treatment strategies. Certain gut microbes, like Enterococcus spp., exhibit remarkable anti-neoplastic and probiotic properties, which can aid in silver nanoparticle (AgNPs) induced reactive oxygen species (ROS)-based CRC treatment. However, the effects of AgNPs on gut microbial metabolism have not been reported thus far. In this study, a detailed systems-level understanding of ROS metabolism in Enterococcus durans (E. durans), a representative gut microbe, was gained using constraint-based modeling, wherein, the critical association between ROS and folate metabolism was established. Experimental studies involving low AgNP concentration treatment of E. durans cultures confirmed these modeling predictions (an increased extracellular folate concentration by 52%, at the 9th h of microbial growth, was observed). Besides, the computational studies established various metabolic pathways involving amino acids, energy metabolites, nucleotides, and SCFAs as the key players in elevating folate levels on ROS exposure. The anti-cancer potential of E. durans was also studied through MTT analysis of HCT 116 cells treated with microbial culture (AgNP treated) supernatant. A decrease in cell viability by 19% implicated the role of microbial metabolites (primarily folate) in causing cell death. The genome-scale modeling approach was then extended to extensively model CRC metabolism, as well as CRC–E. durans interactions in the context of CRC treatment, using tissue-specific metabolic models of CRC and healthy colon. These findings on further validation can facilitate the development of robust and effective cancer therapy.
Keywords	Biology (General) ; QH301-705.5
Subject code	500
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: High carbon (CO2) supply leads to elevated intracellular acetyl CoA levels and increased lipid accumulation in Chlorella vulgaris

Jose, Steffi / G.K. Suraishkumar

Algal research. 2016 Nov., v. 19

2016

Abstract: Carbon dioxide, the sole carbon source in phototrophic cultivation of microalgae, is the limiting factor for photosynthesis due to its low concentration in the atmosphere (0.04% v/v). We postulate that exposure to increased CO2 concentrations can ... ...

Abstract	Carbon dioxide, the sole carbon source in phototrophic cultivation of microalgae, is the limiting factor for photosynthesis due to its low concentration in the atmosphere (0.04% v/v). We postulate that exposure to increased CO2 concentrations can increase the steady-state specific intracellular levels of carbon metabolic intermediates, including, the specific intracellular (si) levels of the precursor for fatty acid synthesis (acetyl CoA (AcCoA)), which in turn, appears to improve lipid accumulation. The effects of higher CO2 concentrations on Chlorella vulgaris were studied. At 2.6% v/v CO2, a 6-fold increase in volumetric lipid production was achieved. A 5.4-fold increase in the specific intracellular neutral lipid level (si-NL) from 9.6 to 52.3mg triolein (TO)/g biomass was also obtained. Si-AcCoA was significantly elevated at 2.6% CO2, and showed a 41, 25 and 27% increase over the air-sparged controls in the lag, log and stationary phases, respectively. Further, we show a quantitative empirical relationship between si-AcCoA and si-NL, which provides a new outlook for the design of strategies to improve lipid accumulation. In addition, favorable biodiesel characteristics were also obtained at higher CO2. Increased si-AcCoA with increase in CO2 concentrations, a related increase in lipid levels, and a quantitative empirical relationship between them have not been reported in the literature thus far.
Keywords	acetyl coenzyme A ; biodiesel ; biomass ; carbon ; carbon dioxide ; Chlorella vulgaris ; fatty acids ; microalgae ; photosynthesis ; triolein
Language	English
Dates of publication	2016-11
Size	p. 307-315.
Publishing place	Elsevier B.V.
Document type	Article
ISSN	2211-9264
DOI	10.1016/j.algal.2016.08.011
Database	NAL-Catalogue (AGRICOLA)

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Article: Effect of light on the kinetics and equilibrium of the textile dye (Reactive Red 120) adsorption by Helianthus annuus hairy roots

Srikantan, Chitra / G.K. Suraishkumar / Smita Srivastava

Bioresource technology. 2018 June, v. 257

2018

Abstract: The study demonstrates for the first time that light influences the adsorption equilibrium and kinetics of a dye by root culture system. The azo dye (Reactive Red 120) adsorption by the hairy roots of H. annuus followed a pseudo first-order kinetic model ...

Abstract	The study demonstrates for the first time that light influences the adsorption equilibrium and kinetics of a dye by root culture system. The azo dye (Reactive Red 120) adsorption by the hairy roots of H. annuus followed a pseudo first-order kinetic model and the adsorption equilibrium parameters were best estimated using Langmuir isotherm. The maximum dye adsorption capacity of the roots increased 6-fold, from 0.26 mg g−1 under complete dark conditions to 1.51 mg g−1 under 16/8 h light/dark photoperiod. Similarly, adsorption rate of the dye and removal (%) also increased in the presence of light, irrespective of the initial concentration of the dye (20–110 mg L−1). The degradation of the azo dye upon adsorption by the hairy roots of H. annuus was also confirmed. In addition, a strategy for simultaneous dye removal and increased alpha-tocopherol (industrially relevant) production by H. annuus hairy root cultures has been proposed and demonstrated.
Keywords	adsorption ; alpha-tocopherol ; azo dyes ; fabrics ; Helianthus annuus ; kinetics ; photoperiod ; roots ; sorption isotherms
Language	English
Dates of publication	2018-06
Size	p. 84-91.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	1065195-0
ISSN	1873-2976 ; 0960-8524
ISSN (online)	1873-2976
ISSN	0960-8524
DOI	10.1016/j.biortech.2018.02.075
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Entrainment of superoxide rhythm by menadione in HCT116 colon cancer cells

Uma Kizhuveetil / Meghana V. Palukuri / Priyanshu Sharma / Devarajan Karunagaran / Raghunathan Rengaswamy / G. K. Suraishkumar

Scientific Reports, Vol 9, Iss 1, Pp 1-

2019 Volume 12

Abstract: Abstract Reactive oxygen species (ROS) are primary effectors of cytotoxicity induced by many anti-cancer drugs. Rhythms in the pseudo-steady-state (PSS) levels of particular intracellular ROS in cancer cells and their relevance to drug effectiveness are ... ...

Abstract	Abstract Reactive oxygen species (ROS) are primary effectors of cytotoxicity induced by many anti-cancer drugs. Rhythms in the pseudo-steady-state (PSS) levels of particular intracellular ROS in cancer cells and their relevance to drug effectiveness are unknown thus far. We report that the PSS levels of intracellular superoxide (SOX), an important ROS, exhibit an inherent rhythm in HCT116 colon cancer cells, which is entrained (reset) by the SOX inducer, menadione (MD). This reset was dependent on the expression of p53, and it doubled the sensitivity of the cells to MD. The period of oscillation was found to have a linear correlation with MD concentration, given by the equation, T, in h = 23.52 − 1.05 [MD concentration in µM]. Further, we developed a mathematical model to better understand the molecular mechanisms involved in rhythm reset. Biologically meaningful parameters were obtained through parameter estimation techniques; the model can predict experimental profiles of SOX, establish qualitative relations between interacting species in the system and serves as an important tool to understand the profiles of various species. The model was also able to successfully predict the rhythm reset in MD treated hepatoma cell line, HepG2.
Keywords	Medicine ; R ; Science ; Q
Subject code	612
Language	English
Publishing date	2019-03-01T00:00:00Z
Publisher	Nature Publishing Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Sustainable Diesel Feedstock: a Comparison of Oleaginous Bacterial and Microalgal Model Systems

Archanaa, S / Steffi Jose / Amitava Mukherjee / G. K. Suraishkumar

BioEnergy research. 2019 Mar., v. 12, no. 1

2019

Abstract: The key to sustainable and commercially viable biodiesel production relies primarily on species selection. Oleaginous species with high biomass productivity, lipid content, and lipid productivity are desirable. High growth rate of the species results in ... ...

Abstract	The key to sustainable and commercially viable biodiesel production relies primarily on species selection. Oleaginous species with high biomass productivity, lipid content, and lipid productivity are desirable. High growth rate of the species results in high biomass productivity, which leads to high lipid productivity. It is known that algal oil technology lacks commercial feasibility predominantly due to low biomass productivity and other factors. The use of a faster-growing organism, such as oleaginous bacteria, could offset this major disadvantage. Thus, the current study analyzes two model oleaginous systems: Rhodococcus opacus PD630 (a bacterium) and Chlorella vulgaris NIOT5 (a microalga) for their growth rate and lipid productivity. It was found that the bacterial growth rate was 25-fold the microalgal growth rate. The bacterium also showed 57-fold higher biomass productivity and 75-fold higher biodiesel productivity. Further, the analysis of a large number of literature data from relevant studies under different cultivation conditions showed that R. opacus PD630 has productivities far higher than various autotrophic microalgae. Similarly, a frequency distribution of data collected from the literature showed that Rhodococcus sp. has productivities in the higher range as compared to heterotrophic microalgae. Thus, bacteria could serve as a better alternative to microalgae toward developing a commercially viable biofuel technology. Further, the biodiesel characterization study showed that the quality of diesel from the bacterium was better than that from the microalga.
Keywords	Chlorella vulgaris ; Rhodococcus opacus ; algal oils ; autotrophic algae ; bacteria ; bacterial growth ; biodiesel ; biomass production ; data collection ; feedstocks ; frequency distribution ; fuel production ; lipid content ; lipids ; microalgae ; models
Language	English
Dates of publication	2019-03
Size	p. 205-216.
Publishing place	Springer US
Document type	Article
ZDB-ID	2424546-X
ISSN	1939-1242 ; 1939-1234
ISSN (online)	1939-1242
ISSN	1939-1234
DOI	10.1007/s12155-018-9948-6
Database	NAL-Catalogue (AGRICOLA)

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Article: Diminishing bioavailability and toxicity of P25 TiO2 NPs during continuous exposure to marine algae Chlorella sp

Thiagarajan, Vignesh / M., Pavani / S., Archanaa / R., Seenivasan / N., Chandrasekaran / G.K., Suraishkumar / Mukherjee, Amitava

Chemosphere. 2019 Oct., v. 233

2019

Abstract: Titanium dioxide nanoparticles (TiO2 NPs) find applications in our day-to-day life because of unique physicochemical properties. Their release into the aquatic environment poses a possible risk to the organisms. However, the continuing exposure of NPs ... ...

Abstract	Titanium dioxide nanoparticles (TiO2 NPs) find applications in our day-to-day life because of unique physicochemical properties. Their release into the aquatic environment poses a possible risk to the organisms. However, the continuing exposure of NPs might reduce their bioavailability to marine organisms owing to aggregation and sedimentation in the aqueous systems thus significantly reducing their toxic impact. In this regard, the present study investigates the effect of continuous exposure of TiO2 NPs to marine microalgae Chlorella sp. under UV-A irradiation through “tanks in series” mode of experiments. In a three-cycle experiment, concentration of TiO2 NPs in the first cycle was fixed at 62.6 μM, and the interacted nanoparticles was subsequently exposed to fresh batches of algae in the next two cycles. After the interaction, the NPs underwent severe aggregation (mean hydrodynamic diameter 3000 ± 18.2 nm after cycle I) leading to gravitational settling in the medium and thus decreased bioavailability. The aggregation can be attributed to interactions between the particles themselves (homo-aggregation) further aggravated by the presence of the algal cells (hetero-aggregation). Cellular viability after cycle I was found to be only 24.2 ± 2.5%, and it was enhanced to 96.5 ± 2.8% after the cycle III in the course of continuous exposure. The results were validated with estimation of oxidative stress markers such as intracellular ROS (total ROS, superoxide and hydroxyl radicals) and LPO after each cycle of exposure. The continuing decrease in the EPS across the cycles further confirmed the diminishing toxicity of the NPs.
Keywords	Chlorella ; aquatic environment ; bioavailability ; hydrodynamics ; hydroxyl radicals ; irradiation ; microalgae ; nanoparticles ; oxidative stress ; physicochemical properties ; risk ; tanks ; titanium dioxide ; toxicity ; ultraviolet radiation ; viability
Language	English
Dates of publication	2019-10
Size	p. 363-372.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	120089-6
ISSN	1879-1298 ; 0045-6535 ; 0366-7111
ISSN (online)	1879-1298
ISSN	0045-6535 ; 0366-7111
DOI	10.1016/j.chemosphere.2019.05.270
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Diminishing bioavailability and toxicity of P25 TiO

Thiagarajan, Vignesh / M, Pavani / S, Archanaa / R, Seenivasan / N, Chandrasekaran / G K, Suraishkumar / Mukherjee, Amitava

Chemosphere

2019 Volume 233, Page(s) 363–372

Abstract: Titanium dioxide nanoparticles ( ... ...

Abstract	Titanium dioxide nanoparticles (TiO
MeSH term(s)	Aquatic Organisms/drug effects ; Aquatic Organisms/metabolism ; Biological Availability ; Chlorella/drug effects ; Chlorella/metabolism ; Environmental Exposure ; Microalgae/drug effects ; Microalgae/metabolism ; Models, Theoretical ; Nanoparticles/chemistry ; Nanoparticles/toxicity ; Oxidative Stress/drug effects ; Particle Size ; Titanium/chemistry ; Titanium/metabolism ; Titanium/toxicity ; Ultraviolet Rays ; Water Pollutants, Chemical/chemistry ; Water Pollutants, Chemical/metabolism ; Water Pollutants, Chemical/toxicity
Chemical Substances	Water Pollutants, Chemical ; titanium dioxide (15FIX9V2JP) ; Titanium (D1JT611TNE)
Language	English
Publishing date	2019-05-31
Publishing country	England
Document type	Journal Article
ZDB-ID	120089-6
ISSN	1879-1298 ; 0045-6535 ; 0366-7111
ISSN (online)	1879-1298
ISSN	0045-6535 ; 0366-7111
DOI	10.1016/j.chemosphere.2019.05.270
Database	MEDical Literature Analysis and Retrieval System OnLINE

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