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  1. Book ; Thesis: Phasing using high intensity free-electron laser radiation

    Galli, Lorenzo

    2014  

    Author's details vorgelegt von Lorenzo Galli
    Language English
    Size xiv, 129 S., Ill., graph. Darst.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Univ., FB Physik, Diss.--Hamburg, 2014
    Note Zsfassung in dt. und engl. Sprache
    Database Former special subject collection: coastal and deep sea fishing

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  2. Article ; Online: Management of the axilla in breast cancer patients: critical review, regional modified Delphi consensus and implementation in the Tuscan breast network.

    Ghilli, Matteo / Becherini, Carlotta / Meattini, Icro / Angiolini, Catia / Bengala, Carmelo / Marconi, Aroldo / Galli, Lorenzo / Angiolucci, Giovanni / Coltelli, Luigi / Borghesi, Simona / Lastrucci, Luciana / Manca, Gianpiero / Bianchi, Simonetta / Doria, Morena / Casella, Donato / Marotti, Lorenza / Amunni, Gianni / Roncella, Manuela

    La Radiologia medica

    2024  

    Abstract: Purpose: Data from recently trials have provided practice-changing recommendations in management of the axilla in early breast cancer (eBC). However, further controversies have been raised, resulting in heterogeneous diffusion of these recommendations. ... ...

    Abstract Purpose: Data from recently trials have provided practice-changing recommendations in management of the axilla in early breast cancer (eBC). However, further controversies have been raised, resulting in heterogeneous diffusion of these recommendations. Our purpose was to obtain a better homogeneity.
    Material and methods: In 2021, the Tuscan Breast Network (TBN) established a consensus with the aim to update recommendations in this area. We performed a literature review on axillary management in eBC patients which led to an expert Delphi consensus aiming to explore the gray areas, build consensus and propose evidence-based suggestions for an appropriate management. Thereafter, we investigate their implementation in clinical practice.
    Results: (1) DCIS patients should have SLN biopsy only in case of mastectomy or in conservative surgery if tumor is in a location that would preclude future nodal sampling or in case of a mass; (2) ALND may be omitted for 1-2 positive SLN patients undergoing BCS in T1-2 tumors with 1-2 SLN positive, eligible for whole-breast irradiation and adjuvant systemic therapies; (3) consider the option of RNI in patients with 1-3 positive lymph nodes and one or more high-risk characteristics; (4) the population identified in 2) should NOT undergo lymph node irradiation as an alternative to axillary surgery and (5) patients with clinically (pre-operatively) positive axilla, or undergoing primary systemic therapy, or outside the criteria reported in 2) must receive additional ALND and/or RT as per local policy.
    Conclusion: This consensus provided a practical tool to stimulate local and national breast surgical and radiotherapy protocols.
    Language English
    Publishing date 2024-04-29
    Publishing country Italy
    Document type Journal Article
    ZDB-ID 205751-7
    ISSN 1826-6983 ; 0033-8362
    ISSN (online) 1826-6983
    ISSN 0033-8362
    DOI 10.1007/s11547-024-01818-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ue I Zs.59: Show issues Location:
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    ab Jg. 2022: Lesesaal (EG)

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  3. Book ; Online ; Thesis: Phasing using high intensity free-electron laser radiation

    Galli, Lorenzo / Chapman, Henry

    2015  

    Title variant Phasierung mittels hochintensiver Roentgenstrahlung aus Freie-Elektronen-Lasern
    Author's details Lorenzo Galli. Betreuer: Henry Chapman
    Language English
    Size Online-Ressource
    Publisher Staats- und Universitätsbibliothek Hamburg
    Publishing place Hamburg
    Document type Book ; Online ; Thesis
    Thesis / German Habilitation thesis Univ. Hamburg, FB Physik, Diss.--Hamburg, 2014
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

    Full text online

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  4. Article ; Online: The clinical impact of pectoral nerve block in an 'enhanced recovery after surgery' program in breast surgery.

    Conti, Duccio / Valoriani, Juri / Ballo, Piercarlo / Pazzi, Maddalena / Gianesello, Lara / Mengoni, Veronica / Criscenti, Valentina / Gemmi, Eleonora / Stera, Caterina / Zoppi, Federica / Galli, Lorenzo / Pavoni, Vittorio

    Pain management

    2023  Volume 13, Issue 10, Page(s) 585–592

    Abstract: Background: ...

    Abstract Background:
    MeSH term(s) Humans ; Female ; Analgesics, Opioid ; Case-Control Studies ; Pain, Postoperative/prevention & control ; Thoracic Nerves ; Breast Neoplasms/surgery
    Chemical Substances Analgesics, Opioid
    Language English
    Publishing date 2023-11-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 2617136-3
    ISSN 1758-1877 ; 1758-1869
    ISSN (online) 1758-1877
    ISSN 1758-1869
    DOI 10.2217/pmt-2023-0063
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Metabolic labelling of a subpopulation of small extracellular vesicles using a fluorescent palmitic acid analogue.

    Barreca, Valeria / Boussadia, Zaira / Polignano, Deborah / Galli, Lorenzo / Tirelli, Valentina / Sanchez, Massimo / Falchi, Mario / Bertuccini, Lucia / Iosi, Francesca / Tatti, Massimo / Sargiacomo, Massimo / Fiani, Maria Luisa

    Journal of extracellular vesicles

    2023  Volume 12, Issue 12, Page(s) e12392

    Abstract: Exosomes are among the most puzzling vehicles of intercellular communication, but several crucial aspects of their biogenesis remain elusive, primarily due to the difficulty in purifying vesicles with similar sizes and densities. Here we report an ... ...

    Abstract Exosomes are among the most puzzling vehicles of intercellular communication, but several crucial aspects of their biogenesis remain elusive, primarily due to the difficulty in purifying vesicles with similar sizes and densities. Here we report an effective methodology for labelling small extracellular vesicles (sEV) using Bodipy FL C16, a fluorescent palmitic acid analogue. In this study, we present compelling evidence that the fluorescent sEV population derived from Bodipy C16-labelled cells represents a discrete subpopulation of small exosomes following an intracellular pathway. Rapid cellular uptake and metabolism of Bodipy C16 resulted in the incorporation of fluorescent phospholipids into intracellular organelles specifically excluding the plasma membrane and ultimately becoming part of the exosomal membrane. Importantly, our fluorescence labelling method facilitated accurate quantification and characterization of exosomes, overcoming the limitations of nonspecific dye incorporation into heterogeneous vesicle populations. The characterization of Bodipy-labelled exosomes reveals their enrichment in tetraspanin markers, particularly CD63 and CD81, and in minor proportion CD9. Moreover, we employed nanoFACS sorting and electron microscopy to confirm the exosomal nature of Bodipy-labelled vesicles. This innovative metabolic labelling approach, based on the fate of a fatty acid, offers new avenues for investigating exosome biogenesis and functional properties in various physiological and pathological contexts.
    MeSH term(s) Extracellular Vesicles/metabolism ; Palmitic Acid/metabolism ; Exosomes/metabolism ; Biological Transport
    Chemical Substances 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene ; Palmitic Acid (2V16EO95H1)
    Language English
    Publishing date 2023-12-10
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2683797-3
    ISSN 2001-3078 ; 2001-3078
    ISSN (online) 2001-3078
    ISSN 2001-3078
    DOI 10.1002/jev2.12392
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Book ; Online ; Thesis: Phasing using high intensity free-electron laser radiation

    Galli, Lorenzo [Verfasser] / Chapman, Henry [Akademischer Betreuer]

    2015  

    Author's details Lorenzo Galli. Betreuer: Henry Chapman
    Keywords Biowissenschaften, Biologie ; Life Science, Biology
    Subject code sg570
    Language English
    Publisher Staats- und Universitätsbibliothek Hamburg
    Publishing place Hamburg
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

    Full text online

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    Inter-library loan at ZB MED

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  7. Article ; Online: Towards RIP using free-electron laser SFX data.

    Galli, Lorenzo / Son, Sang Kil / White, Thomas A / Santra, Robin / Chapman, Henry N / Nanao, Max H

    Journal of synchrotron radiation

    2015  Volume 22, Issue 2, Page(s) 249–255

    Abstract: Here, it is shown that simulated native serial femtosecond crystallography (SFX) cathepsin B data can be phased by rapid ionization of sulfur atoms. Utilizing standard software adopted for radiation-damage-induced phasing (RIP), the effects on both ... ...

    Abstract Here, it is shown that simulated native serial femtosecond crystallography (SFX) cathepsin B data can be phased by rapid ionization of sulfur atoms. Utilizing standard software adopted for radiation-damage-induced phasing (RIP), the effects on both substructure determination and phasing of the number of collected patterns and fluences are explored for experimental conditions already available at current free-electron laser facilities.
    MeSH term(s) Cathepsin B/radiation effects ; Cathepsins/chemistry ; Crystallography, X-Ray/methods ; Data Accuracy ; Electrons ; Humans ; Lasers ; Models, Molecular ; Models, Theoretical ; Protein Conformation/radiation effects ; Radiation Injuries ; Scattering, Radiation ; Sensitivity and Specificity
    Chemical Substances Cathepsins (EC 3.4.-) ; Cathepsin B (EC 3.4.22.1)
    Language English
    Publishing date 2015-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2021413-3
    ISSN 1600-5775 ; 0909-0495
    ISSN (online) 1600-5775
    ISSN 0909-0495
    DOI 10.1107/S1600577514027854
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Recent developments in

    White, Thomas A / Mariani, Valerio / Brehm, Wolfgang / Yefanov, Oleksandr / Barty, Anton / Beyerlein, Kenneth R / Chervinskii, Fedor / Galli, Lorenzo / Gati, Cornelius / Nakane, Takanori / Tolstikova, Alexandra / Yamashita, Keitaro / Yoon, Chun Hong / Diederichs, Kay / Chapman, Henry N

    Journal of applied crystallography

    2016  Volume 49, Issue Pt 2, Page(s) 680–689

    Abstract: ... ...

    Abstract CrystFEL
    Language English
    Publishing date 2016-03-29
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2020879-0
    ISSN 1600-5767 ; 0021-8898
    ISSN (online) 1600-5767
    ISSN 0021-8898
    DOI 10.1107/S1600576716004751
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: XFEL and NMR Structures of Francisella Lipoprotein Reveal Conformational Space of Drug Target against Tularemia.

    Zook, James / Shekhar, Mrinal / Hansen, Debra / Conrad, Chelsie / Grant, Thomas / Gupta, Chitrak / White, Thomas / Barty, Anton / Basu, Shibom / Zhao, Yun / Zatsepin, Nadia / Ishchenko, Andrii / Batyuk, Alex / Gati, Cornelius / Li, Chufeng / Galli, Lorenzo / Coe, Jesse / Hunter, Mark / Liang, Meng /
    Weierstall, Uwe / Nelson, Garret / James, Daniel / Stauch, Benjamin / Craciunescu, Felicia / Thifault, Darren / Liu, Wei / Cherezov, Vadim / Singharoy, Abhishek / Fromme, Petra

    Structure (London, England : 1993)

    2020  Volume 28, Issue 5, Page(s) 540–547.e3

    Abstract: Francisella tularensis is the causative agent for the potentially fatal disease tularemia. The lipoprotein Flpp3 has been identified as a virulence determinant of tularemia with no sequence homology outside the Francisella genus. We report a room ... ...

    Abstract Francisella tularensis is the causative agent for the potentially fatal disease tularemia. The lipoprotein Flpp3 has been identified as a virulence determinant of tularemia with no sequence homology outside the Francisella genus. We report a room temperature structure of Flpp3 determined by serial femtosecond crystallography that exists in a significantly different conformation than previously described by the NMR-determined structure. Furthermore, we investigated the conformational space and energy barriers between these two structures by molecular dynamics umbrella sampling and identified three low-energy intermediate states, transitions between which readily occur at room temperature. We have also begun to investigate organic compounds in silico that may act as inhibitors to Flpp3. This work paves the road to developing targeted therapeutics against tularemia and aides in our understanding of the disease mechanisms of tularemia.
    MeSH term(s) Anti-Bacterial Agents/chemistry ; Anti-Bacterial Agents/pharmacology ; Crystallography, X-Ray/methods ; Databases, Pharmaceutical ; Drug Evaluation, Preclinical/methods ; Francisella tularensis/chemistry ; Francisella tularensis/pathogenicity ; Humans ; Hydrophobic and Hydrophilic Interactions ; Lasers ; Lipoproteins/antagonists & inhibitors ; Lipoproteins/chemistry ; Lipoproteins/genetics ; Molecular Dynamics Simulation ; Molecular Targeted Therapy ; Nuclear Magnetic Resonance, Biomolecular ; Protein Conformation ; Tularemia/drug therapy ; Virulence Factors/chemistry
    Chemical Substances Anti-Bacterial Agents ; Lipoproteins ; Virulence Factors
    Language English
    Publishing date 2020-03-05
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 1213087-4
    ISSN 1878-4186 ; 0969-2126
    ISSN (online) 1878-4186
    ISSN 0969-2126
    DOI 10.1016/j.str.2020.02.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4141: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  10. Article: Towards phasing using high X-ray intensity.

    Galli, Lorenzo / Son, Sang-Kil / Barends, Thomas R M / White, Thomas A / Barty, Anton / Botha, Sabine / Boutet, Sébastien / Caleman, Carl / Doak, R Bruce / Nanao, Max H / Nass, Karol / Shoeman, Robert L / Timneanu, Nicusor / Santra, Robin / Schlichting, Ilme / Chapman, Henry N

    IUCrJ

    2015  Volume 2, Issue Pt 6, Page(s) 627–634

    Abstract: X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can ... ...

    Abstract X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential 'bleaching' of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme is proposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed.
    Language English
    Publishing date 2015-09-30
    Publishing country England
    Document type Journal Article
    ZDB-ID 2754953-7
    ISSN 2052-2525
    ISSN 2052-2525
    DOI 10.1107/S2052252515014049
    Database MEDical Literature Analysis and Retrieval System OnLINE

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