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  1. Book ; Online: Whole-body regeneration

    Blanchoud, Simon / Galliot, Brigitte

    methods and protocols

    (Methods in molecular biology ; 2450)

    2022  

    Title variant Whole body regeneration
    Author's details edited by Simon Blanchoud, Brigitte Galliot
    Series title Methods in molecular biology ; 2450
    Collection
    Language English
    Size 1 Online-Ressource (XVII, 685 Seiten), Illustrationen, Diagramme
    Publisher Springer ; Imprint: Humana
    Publishing place New York
    Publishing country United States
    Document type Book ; Online
    Note Open Access
    HBZ-ID HT021317005
    ISBN 978-1-07-162172-1 ; 9781071621745 ; 9781071621714 ; 1-07-162172-6 ; 1071621742 ; 1071621718
    DOI 10.1007/978-1-0716-2172-1
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Book: Mechanisms of regeneration

    Galliot, Brigitte

    (Current topics in developmental biology ; 108)

    2014  

    Author's details ed. by Brigitte Galliot
    Series title Current topics in developmental biology ; 108
    Collection
    Language English
    Size XVIII, 346, [20] S. : Ill., graph. Darst.
    Edition 1. ed.
    Publisher Elsevier
    Publishing place Amsterdam u.a.
    Publishing country Netherlands
    Document type Book
    HBZ-ID HT018240887
    ISBN 978-0-12-391498-9 ; 0-12-391498-1
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Se 175 -108- verfügbar in Königswinter Königswinter

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  3. Book ; Online: Whole-Body Regeneration

    Bale, Gemma / Taylor, Nathan / Mitra, Subhabrata / Sudakou, Aleh / de Roever, Isabel / Meek, Judith / Robertson, Nicola / Tachtsidis, Ilias / Blanchoud, Simon / Galliot, Brigitte

    Methods and Protocols

    (Methods in Molecular Biology)

    2022  

    Series title Methods in Molecular Biology
    Keywords Cellular biology (cytology) ; Genetics (non-medical) ; immune response ; cell sorting ; stem cells ; cell isolation ; apoptosis ; Open Access
    Language 0|e
    Size 1 electronic resource (685 pages)
    Publisher Springer Nature
    Publishing place New York, NY
    Document type Book ; Online
    Note English ; Open Access
    HBZ-ID HT021614846
    ISBN 9781071621721 ; 1071621726
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  4. Article ; Online: Combining RNAi-Mediated β-Catenin Inhibition and Reaggregation to Study Hydra Whole-Body Regeneration.

    Vogg, Matthias Christian / Galliot, Brigitte

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2450, Page(s) 635–647

    Abstract: In addition to its ability to regenerate any amputated body part, the Hydra freshwater polyp shows the amazing ability to regenerate as a full polyp after a complete dissociation of its tissues. The developmental processes at work in reaggregates ... ...

    Abstract In addition to its ability to regenerate any amputated body part, the Hydra freshwater polyp shows the amazing ability to regenerate as a full polyp after a complete dissociation of its tissues. The developmental processes at work in reaggregates undergoing whole-body regeneration can be investigated at the molecular level by RNA interference (RNAi). Here we provide a protocol that combines β-catenin RNAi with reaggregation. This protocol serves as a basis to generate "RNAi-reaggregates," followed by the extraction of high-quality RNA for the precise quantification of gene expression by real-time PCR. This protocol is efficient, providing both a molecular signature, with the significant downregulation of β-catenin and Wnt3, as well as a robust phenotype, the lack of axis formation, which is observed in all reaggregates.
    MeSH term(s) Animals ; Hydra/genetics ; Hydra/metabolism ; RNA Interference ; beta Catenin/genetics ; beta Catenin/metabolism
    Chemical Substances beta Catenin
    Language English
    Publishing date 2022-04-28
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2172-1_34
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Studying Stem Cell Biology in Intact and Whole-Body Regenerating Hydra by Flow Cytometry.

    Buzgariu, Wanda / Aubry-Lachainaye, Jean-Pierre / Galliot, Brigitte

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2450, Page(s) 373–398

    Abstract: The freshwater Hydra polyp is a versatile model to study whole-body regeneration from a developmental as well as a cellular point of view. The outstanding regenerative capacities of Hydra are based on its three populations of adult stem cells located in ... ...

    Abstract The freshwater Hydra polyp is a versatile model to study whole-body regeneration from a developmental as well as a cellular point of view. The outstanding regenerative capacities of Hydra are based on its three populations of adult stem cells located in the central body column of the animal. There, these three populations, gastrodermal epithelial, epidermal epithelial, and interstitial, continuously cycle in homeostatic conditions, and their activity is locally regulated after mid-gastric bisection. Moreover, they present an unusual cycling behavior with a short G1 phase and a pausing in G2. This particular cell cycle has been studied for a long time with classical microscopic methods. We describe here two flow cytometry methods that provide accurate and reproducible quantitative data to monitor cell cycle regulation in homeostatic and regenerative contexts. We also present a cell sorting procedure based on flow cytometry, whereby stem cells expressing a fluorescent reporter protein in transgenic lines can be enriched for use in applications such as transcriptomic, proteomic, or cell cycle analysis.
    MeSH term(s) Animals ; Cell Cycle/physiology ; Flow Cytometry ; Hydra ; Proteomics ; Stem Cells
    Language English
    Publishing date 2022-04-28
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2172-1_20
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Book: Mechanisms of regeneration

    Galliot, Brigitte

    (Current topics in developmental biology, ; v. 108)

    2014  

    Author's details edited by Brigitte Galliot
    Series title Current topics in developmental biology, ; v. 108
    MeSH term(s) Regeneration/physiology
    Language English
    Dates of publication 2014-2014
    Size xviii, 346 pages, 20 pages of unnumbered plates :, illustrations (some color) ;, 24 cm.
    Edition First edition.
    Document type Book
    ISBN 9780123914989 ; 0123914981
    Database Catalogue of the US National Library of Medicine (NLM)

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  7. Article ; Online: Mechanisms of regeneration. Preface.

    Galliot, Brigitte

    Current topics in developmental biology

    2014  Volume 108, Page(s) xiii–xviii

    MeSH term(s) Animals ; Cell Death ; Developmental Biology ; Humans ; Models, Theoretical ; Plant Physiological Phenomena ; Regeneration/physiology
    Language English
    Publishing date 2014
    Publishing country United States
    Document type Introductory Journal Article
    ISSN 1557-8933 ; 0070-2153
    ISSN (online) 1557-8933
    ISSN 0070-2153
    DOI 10.1016/B978-0-12-391498-9.10000-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Hydra, a fruitful model system for 270 years.

    Galliot, Brigitte

    The International journal of developmental biology

    2012  Volume 56, Issue 6-8, Page(s) 411–423

    Abstract: The discovery of Hydra regeneration by Abraham Trembley in 1744 promoted much scientific curiosity thanks to his clever design of experimental strategies away from the natural environment. Since then, this little freshwater cnidarian polyp flourished as ... ...

    Abstract The discovery of Hydra regeneration by Abraham Trembley in 1744 promoted much scientific curiosity thanks to his clever design of experimental strategies away from the natural environment. Since then, this little freshwater cnidarian polyp flourished as a potent and fruitful model system. Here, we review some general biological questions that benefitted from Hydra research, such as the nature of embryogenesis, neurogenesis, induction by organizers, sex reversal, symbiosis, aging, feeding behavior, light regulation, multipotency of somatic stem cells, temperature-induced cell death, neuronal transdifferentiation, to cite only a few. To understand how phenotypes arise, theoricists also chose Hydra to model patterning and morphogenetic events, providing helpful concepts such as reaction-diffusion, positional information, and autocatalysis combined with lateral inhibition. Indeed, throughout these past 270 years, scientists used transplantation and grafting experiments, together with tissue, cell and molecular labelings, as well as biochemical procedures, in order to establish the solid foundations of cell and developmental biology. Nowadays, thanks to transgenic, genomic and proteomic tools, Hydra remains a promising model for these fields, but also for addressing novel questions such as evolutionary mechanisms, maintenance of dynamic homeostasis, regulation of stemness, functions of autophagy, cell death, stress response, innate immunity, bioactive compounds in ecosystems, ecotoxicant sensing and science communication.
    MeSH term(s) Animals ; Apoptosis ; Cell Transdifferentiation ; Developmental Biology ; Hydra/cytology ; Hydra/genetics ; Hydra/physiology ; Models, Animal ; Models, Biological ; Morphogenesis ; Multipotent Stem Cells ; Regeneration ; Signal Transduction
    Language English
    Publishing date 2012
    Publishing country Spain
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 1036070-0
    ISSN 1696-3547 ; 0214-6282
    ISSN (online) 1696-3547
    ISSN 0214-6282
    DOI 10.1387/ijdb.120086bg
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3202: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  9. Article ; Online: Preface: the hydra model system.

    Galliot, Brigitte

    The International journal of developmental biology

    2012  Volume 56, Issue 6-8, Page(s) 407–409

    Abstract: The freshwater Hydra polyp emerged as a model system in 1741 when Abraham Trembley not only discovered its amazing regenerative potential, but also demonstrated that experimental manipulations pave the way to research in biology. Since then, Hydra ... ...

    Abstract The freshwater Hydra polyp emerged as a model system in 1741 when Abraham Trembley not only discovered its amazing regenerative potential, but also demonstrated that experimental manipulations pave the way to research in biology. Since then, Hydra flourished as a potent and fruitful model system to help answer questions linked to cell and developmental biology, as such as the setting up of an organizer to regenerate a complex missing structure, the establishment and maintainance of polarity in a multicellular organism, the development of mathematical models to explain the robust developmental rules observed in this animal, the maintainance of stemness and multipotency in a highly dynamic environment, the plasticity of differentiated cells, to name but a few. However the Hydra model system is not restricted to cell and developmental biology; during the past 270 years it has also been heavily used to investigate the relationships between Hydra and its environment, opening new horizons concerning neurophysiology, innate immunity, ecosystems, ecotoxicology, symbiosis...
    MeSH term(s) Animals ; Hydra/physiology ; Models, Animal ; Models, Biological ; Regeneration
    Language English
    Publishing date 2012
    Publishing country Spain
    Document type Journal Article
    ZDB-ID 1036070-0
    ISSN 1696-3547 ; 0214-6282
    ISSN (online) 1696-3547
    ISSN 0214-6282
    DOI 10.1387/ijdb.120094bg
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3202: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  10. Article ; Online: Injury-induced asymmetric cell death as a driving force for head regeneration in Hydra.

    Galliot, Brigitte

    Development genes and evolution

    2012  Volume 223, Issue 1-2, Page(s) 39–52

    Abstract: The freshwater Hydra polyp provides a unique model system to decipher the mechanisms underlying adult regeneration. Indeed, a single cut initiates two distinct regenerative processes, foot regeneration on one side and head regeneration on the other side, ...

    Abstract The freshwater Hydra polyp provides a unique model system to decipher the mechanisms underlying adult regeneration. Indeed, a single cut initiates two distinct regenerative processes, foot regeneration on one side and head regeneration on the other side, the latter relying on the rapid formation of a local head organizer. Two aspects are discussed here: the asymmetric cellular remodeling induced by mid-gastric bisection and the signaling events that trigger head organizer formation. In head-regenerating tips (but not in foot ones), a wave of cell death takes place immediately, leading the apoptotic cells to transiently release Wnt3 and activate the β-catenin pathway in the neighboring cycling cells to push them through mitosis. This process, which mimics the apoptosis-induced compensatory proliferation process deciphered in Drosophila larvae regenerating their discs, likely corresponds to an evolutionarily conserved mechanism, also at work in Xenopus tadpoles regenerating their tail or mice regenerating their skin or liver. How is this process generated in Hydra? Several studies pointed to the necessary activation of the extracellular signal-regulated kinase (ERK) 1-2 and mitogen-activated protein kinase (MAPK) pathways during early head regeneration. Indeed inhibition of ERK 1-2 or knockdown of RSK, cAMP response element-binding protein (CREB), and CREB-binding protein (CBP) prevent injury-induced apoptosis and head regeneration. The current scenario involves an asymmetric activation of the MAPK/CREB pathway to trigger injury-induced apoptosis in the interstitial cells and in the epithelial cells a CREB/CBP-dependent transcriptional activation of early genes essential for head-organizing activity as wnt3, HyBra1, and prdl-a. The question now is how bisection in the rather uniform central region of the polyp can generate this immediately asymmetric signaling.
    MeSH term(s) Animals ; Cell Death ; Hydra/cytology ; Hydra/physiology ; Models, Animal ; Regeneration ; Signal Transduction ; Stem Cells/cytology
    Language English
    Publishing date 2012-07-26
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 1327962-2
    ISSN 1432-041X ; 0949-944X
    ISSN (online) 1432-041X
    ISSN 0949-944X
    DOI 10.1007/s00427-012-0411-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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