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Article ; Online: Introducing the f

Gamal, Mahmoud / Ibrahim, Marwa A

2024 Volume 25, Issue 1, Page(s) 17

Abstract: Background: qPCR is a widely used technique in scientific research as a basic tool in gene expression analysis. Classically, the quantitative endpoint of qPCR is the threshold cycle (C: Results: The performance of the new f: Conclusions: We ... ...

Abstract	Background: qPCR is a widely used technique in scientific research as a basic tool in gene expression analysis. Classically, the quantitative endpoint of qPCR is the threshold cycle (C Results: The performance of the new f Conclusions: We recommend using the f
Language	English
Publishing date	2024-01-11
Publishing country	England
Document type	Journal Article
ZDB-ID	2041484-5
ISSN	1471-2105 ; 1471-2105
ISSN (online)	1471-2105
ISSN	1471-2105
DOI	10.1186/s12859-024-05630-y
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Article: Platelet Glycoprotein IIIa

Mohamed Ahmed, Mohanad Altayeb / Ali, Elshazali Widaa / Alimairi, Gamal Mahmoud

Journal of clinical medicine research

2024 Volume 16, Issue 2-3, Page(s) 75–80

Abstract: Background: Genetic polymorphisms of platelet glycoprotein IIIa (: Methods: A total of 200 participants were recruited for the study, 100 non-diabetic patients with myocardial infarction and 100 apparently healthy volunteers as a control group. : ... ...

Abstract	Background: Genetic polymorphisms of platelet glycoprotein IIIa ( Methods: A total of 200 participants were recruited for the study, 100 non-diabetic patients with myocardial infarction and 100 apparently healthy volunteers as a control group. Results: The distribution of Conclusions: The
Language	English
Publishing date	2024-03-16
Publishing country	Canada
Document type	Journal Article
ZDB-ID	2548987-2
ISSN	1918-3011 ; 1918-3003
ISSN (online)	1918-3011
ISSN	1918-3003
DOI	10.14740/jocmr5094
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Article ; Online: Antibiotic use numeracy: Developing the infectious numeracy test (INT).

Hasan, Sanah / Gamal, Mahmoud / Samorinha, Catarina / Hassan, Nageeb AbdulGalil

Research in social & administrative pharmacy : RSAP

2022 Volume 18, Issue 9, Page(s) 3580–3587

Abstract: Background: Inadequate numeracy is a barrier to patients' understanding of their health. No measure of numeracy related to antibiotic use is available.: Objective: to develop and validate a measure of numeracy of antibiotic use, and to make this ... ...

Abstract	Background: Inadequate numeracy is a barrier to patients' understanding of their health. No measure of numeracy related to antibiotic use is available. Objective: to develop and validate a measure of numeracy of antibiotic use, and to make this measure available in the Arabic language and context. Methods: Best practices for developing, translating and validating scales were consulted to develop an anonymous survey administered on SurveyMonkey. Ten open-ended questions for which participants provided an answer assessed basic numeracy covering fractions, percentages, proportionality, measurement, and estimation, contextualized to real life situations. A panel of five experts, and 10 cognitive debriefings provided face and content validity for the tool. Individuals ≥18 years, who had mastery of Arabic or English were included. Exploratory factor analysis was performed to characterize the psychometric properties of the items, using principal-component analysis. Convergent validity was established by comparing numeracy scores with the antibiotic knowledge scale scores. Differences in numeracy scores according to sociodemographic variables were tested using multivariate analyses of variance. Results: Two hundred fifty-four responses were obtained, giving a response rate of 63.5%. A two-factor structure which explained 44.2% of the variance evolved: "Mathematical knowledge and problem-solving skills" and "Numeracy-related practices and experience". An acceptable reliability of Cronbach's alpha coefficient = 0.713. Higher numeracy scores were significantly correlated with the scores of the antibiotic knowledge (r = 0.205, p < 0.01) and factor one within the tool: "side effects and resistance" (r = 0.162, p = 0.05). Participants with University/College education presented higher levels of numeracy-related practices and experience than those with high-school education. Likewise, participants with medical insurance presented with higher numeracy scores than those without. Conclusions: A valid and reliable measure of antibiotic numeracy in English and Arabic was created. The tool could identify patients with low numeracy who are candidates for targeted interventions and intensive education on appropriate antibiotic use.
MeSH term(s)	Anti-Bacterial Agents/therapeutic use ; Health Literacy ; Humans ; Psychometrics ; Reproducibility of Results ; Surveys and Questionnaires ; Translating
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2022-03-17
Publishing country	United States
Document type	Journal Article
ZDB-ID	2192059-X
ISSN	1934-8150 ; 1551-7411
ISSN (online)	1934-8150
ISSN	1551-7411
DOI	10.1016/j.sapharm.2022.03.007
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Article ; Online: Comparative Study Between Single Layer and Double Layer Graft Technique in Tympanoplasty for Subtotal Tympanic Membrane Perforation

Ahmed Abdel Rahman Abdel Aziz / Montaser Abdel Salam Hafez / Ashraf Gamal Mahmoud / AbdelMoneim H. Hamad

International Archives of Otorhinolaryngology (2023)

2023

Abstract: Introduction Tympanoplasty is the main treatment of mucosal type of chronic suppurative otitis media. Objective The aim of the present study was to compare clinical outcomes in terms of healing and audiological outcomes of two groups. The authors used ... ...

Abstract	Introduction Tympanoplasty is the main treatment of mucosal type of chronic suppurative otitis media. Objective The aim of the present study was to compare clinical outcomes in terms of healing and audiological outcomes of two groups. The authors used single layer graft (perichondrium with cartilage island) graft in one group and double layer grafts (perichondrium cartilage island composite plus temporalis fascia) were used in the second group. Methods Forty patients complained of chronic suppurative otitis media safe type with subtotal perforation subdivided into two groups. The first group author used single-layer perichondrium with cartilage island graft (composite graft) while in the second group authors used double graft in the form of perichondrium/cartilage island (composite graft) plus temporalis fascia. Results There was no significant difference in the mean Air bone gap (ABG) between pre- and post-operative audiometry in subjects who had single layer tympanoplasty. There was a significant difference in the mean ABG between pre- and postoperative audiometry in subjects who had double layer tympanoplasty. There was a significant difference in in the mean ABG differences between subjects who had single layer tympanoplasty and double layer tympanoplasty. Also, there was a significant difference in the healing of the tympanic membrane between subjects who had single layer tympanoplasty and double layer tympanoplasty. Conclusion Tympanoplasty by double graft (temporalis fascia and tragal cartilage/perichondrium) achieved a considerable improvement in healing of the tympanic membrane ™ with lower risk for residual perforation or graft rejection.
Keywords	otitis media ; ear diseases ; hearing disorders ; Medicine ; R ; Otorhinolaryngology ; RF1-547
Subject code	610
Language	English
Publishing date	2023-03-01T00:00:00Z
Publisher	Thieme Revinter Publicações Ltda.
Document type	Article ; Online
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Article ; Online: Effect of pre-pubertal growth rate of Sohagi ram lambs on some physiological parameters and sexual behavioral patterns at puberty.

Elaref, Mohamed Youssef / Solouma, Gamal Mahmoud / Abdel-Latif, Doaa Ahmed

Animal reproduction

2021 Volume 18, Issue 4, Page(s) e20210104

Abstract: Thirty healthy Sohagi ram lambs with an average age of 188.6±7.3 days were used to study the effect of pre-pubertal growth rate on some physiological parameters and sexual behavioral patterns at puberty. Ram lambs were divided into three groups (10 ... ...

Abstract	Thirty healthy Sohagi ram lambs with an average age of 188.6±7.3 days were used to study the effect of pre-pubertal growth rate on some physiological parameters and sexual behavioral patterns at puberty. Ram lambs were divided into three groups (10 animals per each group) according to the previous growth rate until 6 months of age. Groups were marked as fast, medium and slow growing. Animal groups were housed in closed barns with access to an open area. Results showed that age and weight of ram lambs at puberty were significantly affected (P<0.05) by the pre-pubertal growth rate. Ram lambs in the fast growing group were reached to onset puberty firstly at 272.6 days with body weight (BW) 37.1 kg on average then ram lambs in medium group (284.8 days with BW 32.7 kg), while ram lambs in slow growing group were the last (314.1 days with BW 32.5 kg). Blood‎ testosterone‎ concentration at puberty was not significantly different among growing groups (1.494± 0.03 ng/ml on average, ranged from 1.287 to 1.902 ng/ml). Testes measurements from 6 months of age until puberty show that ram lambs in fast growing group had the highest values of testes length, circumference and volume followed by those in medium and slow growing group. Sexual behavioral observation showed that flehmen and mounting behavior were significantly higher for ram lambs in fast growing group (5.63 and 6.75 number/12h) than slow growing group (4.25 and 5.38 number/12h) while in medium growing group were intermediate (4.88 and 5.88 number/12h). From these findings, could be concluded that age, weight and sexual behavioral patterns of Sohagi ram lambs at puberty were affected by pre-pubertal growth rate, and the breeders should strive to achieve good growth rates for their lambs before puberty which led to improving reproductive performance.
Language	English
Publishing date	2021-12-01
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2227229-X
ISSN	1984-3143 ; 1984-3143
ISSN (online)	1984-3143
ISSN	1984-3143
DOI	10.1590/1984-3143-AR2021-0104
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Article: ER Stress in COVID-19 and Parkinson's Disease: In Vitro and In Silico Evidences.

Chaudhry, Zahara L / Gamal, Mahmoud / Ferhati, Ingrid / Warda, Mohamad / Ahmed, Bushra Y

Brain sciences

2022 Volume 12, Issue 4

Abstract: The outbreak of COVID-19 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) signifies a serious worldwide concern to public health. Both transcriptome and proteome of SARS-CoV-2-infected cells synergize the progression of infection in ...

Abstract	The outbreak of COVID-19 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) signifies a serious worldwide concern to public health. Both transcriptome and proteome of SARS-CoV-2-infected cells synergize the progression of infection in host, which may exacerbate symptoms and/or progression of other chronic diseases such as Parkinson's disease (PD). Oxidative stress is a well-known cause of endoplasmic reticulum (ER) stress observed in both SARS-CoV-2 and PD. In the current study, we aimed to explore the influence of PKR-like ER kinase (PERK) stress pathway under SARS-CoV-2-mediated infection and in human cell model of PD. Furthermore, we investigated whether they are interconnected and if the ER stress inhibitors could inhibit cell death and provide cellular protection. To achieve this aim, we have incorporated in silico analysis obtained from gene set enrichment analysis (GSEA), a literature review and laboratory data. The neurotoxin, 6-hydroxy dopamine (6OHDA), was used to mimic the biochemical and neuropathological characteristics of PD by inducing oxidative stress in dopamine-containing neurons differentiated from ReNVM cell line (dDCNs). Furthermore, we explored if ER stress influences activation of caspases-2, -4 and -8 in SARS-CoV-2 and in stressed dDCNs. Our laboratory data using Western blot, immunocytochemistry and 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) analyses indicated that 6OHDA-induced toxicity triggered activation of caspases-2, -4 and -8 in dDCNs. Under SARS-CoV-2 infection of different cell types, GSEA revealed cell-specific sensitivities to oxidative and ER stresses. Cardiomyocytes and type II alveolar epithelial-like cells were more vulnerable to oxidative stress than neural cells. On the other side, only cardiomyocytes activated the unfolded protein response, however, the PERK pathway was operative in both cardiomyocytes and neural cells. In addition, caspase-4 activation by a SARS-CoV-2 was observed via in silico analyses. These results demonstrate that the ER stress pathway under oxidative stress in SARS-CoV-2 and PD are interconnected using diverse pathways. Furthermore, our results using the ER stress inhibitor and caspase specific inhibitors provided cellular protection suggesting that the use of specific inhibitors can provide effective therapeutic approaches for the treatment of COVID-19 and PD.
Language	English
Publishing date	2022-04-16
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2651993-8
ISSN	2076-3425
ISSN	2076-3425
DOI	10.3390/brainsci12040507
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Article ; Online: Effect of in-ovo inoculation of betaine on hatchability, serum antioxidant levels, muscle gene expression and intestinal development of broiler chicks.

El-Shater, Saad N / Abo-El-Sooud, Khaled / Tolba, Ayman / Gamal, Mahmoud / Awad, Mohamed A / Ibrahim, Moataz / Tayeh, Mahmoud / Swielim, Gamal A

Journal of animal physiology and animal nutrition

2024

Abstract: This study investigated the effects of in-ovo inoculation of betaine on hatchability, hatching weight, and intestinal development, as well as serum and expression levels of some antioxidants in the posthatched chicks. A total of 350 fertile eggs of ... ...

Abstract	This study investigated the effects of in-ovo inoculation of betaine on hatchability, hatching weight, and intestinal development, as well as serum and expression levels of some antioxidants in the posthatched chicks. A total of 350 fertile eggs of Hubbard efficiency plus breeder's flock were incubated at normal incubation temperature (37.5°C) and randomly assembled into 3 groups with 4 replicates, and 25 eggs per each. The experimental groups were allocated as noninjected control group (CN), diluent-injected group (CP, 0.1 mL saline), and betaine-injected group (B, 2.5 mg in 0.1 mL saline). The injections were performed in the air cells of the eggs on the 12th day of the embryonic phase. Hatchability percentage, hatching weight, serum-reduced glutathione (GSH), and superoxide dismutase (SOD) were estimated in 7-day-old chicks. Moreover, expression levels of the nuclear factor erythroid 2-related factor 2 (Nrf2) and SOD were determined in the breast skeletal muscles of chicks. Jejunum histo-morphometric analysis was assessed with computerised morphometric measurements. The results revealed that the hatchability percentage was not influenced by in-ovo injection of betaine or vehicle while betaine significantly increased the hatchling's weight of chicks. Moreover, there were a significant increase in SOD and Nrf2 mRNA expression levels. In-ovo injection of betaine significantly induced positive effects on intestinal morphometry by ameliorating the jejunal villus length, the ratio of villus height to villus width, and absorptive surface area.
Language	English
Publishing date	2024-02-14
Publishing country	Germany
Document type	Journal Article
ZDB-ID	232729-6
ISSN	1439-0396 ; 0044-3565 ; 0931-2439
ISSN (online)	1439-0396
ISSN	0044-3565 ; 0931-2439
DOI	10.1111/jpn.13938
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Article ; Online: Methanolic Extract of Leathery Murdah, Terminalia coriacea (Roxb.) Wight and Arn. Leaves Exhibits Anti-Inflammatory Activity in Acute and Chronic Models.

Safwan Ali Khan, Mohammed / Khatoon, Nishath / Al-Sanea, Mohammad M / Gamal Mahmoud, Mohamed / Rahman, Hidayat Ur

Medical principles and practice : international journal of the Kuwait University, Health Science Centre

2018 Volume 27, Issue 3, Page(s) 267–271

Abstract: Objective: The aim of the present study was to evaluate anti-inflammatory activity of methanolic extract of Terminalia coriacea.: Materials and methods: A methanolic extract of T. coriacea leaves was studied in albino Wistar rats with carrageenan- ... ...

Abstract	Objective: The aim of the present study was to evaluate anti-inflammatory activity of methanolic extract of Terminalia coriacea. Materials and methods: A methanolic extract of T. coriacea leaves was studied in albino Wistar rats with carrageenan-induced paw edema, an acute model, and cotton pellet-induced granuloma, a chronic model, at 3 oral test doses (125, 250, and 500 mg/kg). Aspirin 100 mg/kg was used as a positive control. Paw volume and wet and dry weights of cotton pellets were determined. The data were analyzed by one-way ANOVA followed by Dunnett's multiple comparison test. Results: The test extract at doses of 125 and 250 mg/kg decreased paw volume and wet and dry weights of cotton pellets. The highest test dose (500 mg/kg) displayed a response comparable to that of the standard drug (p < 0.01) on paw volume. The extract produced similar (p < 0.05) decrease in wet weight of the cotton pellet at 125 and 250 mg/kg, whereas the effect of 500 mg/kg of the extract was comparable to that of aspirin 100 mg/kg (p < 0.01). The extract of T. coriacea at 500 mg/kg induced the most significant (p < 0.01) effect on wet weight of granulomatous tissue. Conclusion: The methanolic extract of T. coriacea leaves successfully decreased paw edema as well as dry and wet weights of granulomatous tissue in both acute and chronic inflammatory models thus confirming the anti- edematogenic, antitransudative, and antiproliferative properties of T. coriacea.
MeSH term(s)	Animals ; Anti-Inflammatory Agents/pharmacology ; Aspirin/pharmacology ; Disease Models, Animal ; Dose-Response Relationship, Drug ; Edema/chemically induced ; Edema/drug therapy ; Granuloma/chemically induced ; Granuloma/drug therapy ; Male ; Phytotherapy/methods ; Plant Extracts/pharmacology ; Rats ; Rats, Wistar ; Terminalia
Chemical Substances	Anti-Inflammatory Agents ; Plant Extracts ; Aspirin (R16CO5Y76E)
Language	English
Publishing date	2018-03-07
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	645108-1
ISSN	1423-0151 ; 1011-7571
ISSN (online)	1423-0151
ISSN	1011-7571
DOI	10.1159/000488199
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Article ; Online: Vector Analysis of Femtosecond Laser-Assisted Arcuate Keratotomy for Post-Keratoplasty Astigmatic Correction.

Elzarga, Ali A A / Osman, Amr A / Gamal, Mahmoud / Khafagy, Mohamed M / Osman, Ihab S

Ophthalmic research

2019 Volume 62, Issue 3, Page(s) 150–156

Abstract: Purpose: To study the astigmatic correction of high post-keratoplasty astigmatism using Femtosecond laser (FSL)-assisted Arcuate Keratotomy (FS-AK).: Methods: A prospective interventional cohort study. We enrolled 17 eyes with high degree of ... ...

Abstract	Purpose: To study the astigmatic correction of high post-keratoplasty astigmatism using Femtosecond laser (FSL)-assisted Arcuate Keratotomy (FS-AK). Methods: A prospective interventional cohort study. We enrolled 17 eyes with high degree of irregular astigmatism, scheduled for FS-AK. FSL was used to perform paired arcuate incisions 1.00 mm inside the graft. Patients' uncorrected visual acuity (UCVA), best corrected visual acuity (BCVA), and astigmatic change were recorded and followed up to 1 year after surgery. Vector analysis using Alpins' method was done to analyze the astigmatic correction. Results: FS-AK reduced the refractive astigmatism at final follow-up visit at 12 months (p = 0.0008, repeated-measures analysis of variance [ANOVA]). The procedure improved the UCVA over the follow-up period (p = 0.007, repeated-measures ANOVA), with a similar effect on the BCVA (p = 0.046, repeated-measures ANOVA). There was a mild correlation between the target-induced astigmatism and the surgically induced astigmatism (R2 = 0.245) with a tendency to overcorrect more than under correct the astigmatism. A constant rotational error in the counterclockwise direction was also detected. Conclusions: FS-AK improves the visual outcome and reduces the refractive cylinder in post-penetrating keratoplasty astigmatism. The predictability of astigmatism correction was variable in reducing post-keratoplasty astigmatism. Refinement of the treatment nomogram for such cases is highly recommended.
MeSH term(s)	Adolescent ; Adult ; Analysis of Variance ; Astigmatism/surgery ; Child ; Female ; Humans ; Keratoplasty, Penetrating/adverse effects ; Keratotomy, Radial/methods ; Laser Therapy/methods ; Male ; Middle Aged ; Postoperative Complications/surgery ; Prospective Studies ; Visual Acuity
Language	English
Publishing date	2019-06-05
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	205708-6
ISSN	1423-0259 ; 0030-3747
ISSN (online)	1423-0259
ISSN	0030-3747
DOI	10.1159/000499859
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Article: Double-Fermented Soybean Meal Totally Replaces Soybean Meal in Broiler Rations with Favorable Impact on Performance, Digestibility, Amino Acids Transporters and Meat Nutritional Value.

Abdel-Raheem, Sherief M / Mohammed, El Said Yehia / Mahmoud, Rania Elsaid / El Gamal, Mahmoud Fathy / Nada, Hend S / El-Ghareeb, Waleed Rizk / Marzok, Mohamed / Meligy, Ahmed M A / Abdulmohsen, Mohamad / Ismail, Hesham / Ibrahim, Doaa / Kishawy, Asmaa T Y

Animals : an open access journal from MDPI

2023 Volume 13, Issue 6

Abstract: Inclusion of microbial fermented soybean meal in broiler feed has induced advantageous outcomes for their performance and gastrointestinal health via exhibiting probiotic effects. In this study, soybean meal (SBM) was subjected to double-stage microbial ... ...

Abstract	Inclusion of microbial fermented soybean meal in broiler feed has induced advantageous outcomes for their performance and gastrointestinal health via exhibiting probiotic effects. In this study, soybean meal (SBM) was subjected to double-stage microbial fermentation utilizing functional metabolites of fungi and bacteria. In broiler diet, DFSBM replaced SBM by 0, 25, 50 and 100%. DFSBM was reported to have higher protein content and total essential, nonessential and free amino acids (increased by 3.67%, 12.81%, 10.10% and 5.88-fold, respectively, compared to SBM). Notably, phytase activity and lactic acid bacteria increased, while fiber, lipid and trypsin inhibitor contents were decreased by 14.05%, 38.24% and 72.80%, respectively, in a diet containing 100% DFSBM, compared to SBM. Improved growth performance and apparent nutrient digestibility, including phosphorus and calcium, and pancreatic digestive enzyme activities were observed in groups fed higher DFSBM levels. In addition, higher inclusion levels of DFSBM increased blood immune response (IgG, IgM, nitric oxide and lysozyme levels) and liver antioxidant status. Jejunal amino acids- and peptide transporter-encoding genes (LAT1, CAT-1, CAT-2, PepT-1 and PepT-2) were upregulated with increasing levels of DFSBM in the ration. Breast muscle crude protein, calcium and phosphorus retention were increased, especially at higher inclusion levels of DFSBM. Coliform bacteria load was significantly reduced, while lactic acid bacteria count in broiler intestines was increased with higher dietary levels of DFSBM. In conclusion, replacement of SBM with DFSBM positively impacted broiler chicken feed utilization and boosted chickens' amino acid transportation, in addition to improving the nutritional value of their breast meat.
Language	English
Publishing date	2023-03-11
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2606558-7
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani13061030
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