LIVIVO - Search results -

Search results

Result 1 - 10 of total 15

Search options

Article ; Online: The prevalence of Dupuytren's disease in patients with diabetes mellitus.

Ganesan, Sandhya / Tantone, Ryan P / Komatsu, David E / Hurst, Lawrence C

Communications medicine

2023 Volume 3, Issue 1, Page(s) 96

Abstract: Background: Dupuytren's disease (DD) is a fibroproliferative hand disorder associated with various medical conditions, including diabetes mellitus (DM). The reported prevalence of DM among DD patients varies widely, primarily due to small sample sizes ... ...

Abstract	Background: Dupuytren's disease (DD) is a fibroproliferative hand disorder associated with various medical conditions, including diabetes mellitus (DM). The reported prevalence of DM among DD patients varies widely, primarily due to small sample sizes in previous studies. Methods: This was a retrospective cohort study using data from the TriNetX Research Database. We analyzed the overall prevalence of DD between 2010 and 2020, comparing the DM, type 1 diabetes mellitus (T1DM), and type 2 diabetes mellitus (T2DM) cohorts. Within the DM group, patients were further categorized based on hemoglobin A1c (HbA1c) values and prescribed anti-diabetic agents (insulin or metformin). We compared the prevalence of DD diagnosis in each group using prevalence ratios and differences. Results: There is a higher prevalence of DD in patients with T2DM than in patients with T1DM (relative risk [RR]: 1.641; 95% confidence interval [CI]: [1.356, 1.986]). Among patients with diabetes, there is a higher prevalence of DD in those taking insulin compared to those taking metformin (RR: 0.801, 95% CI: [0.774, 0.83]). The prevalence of DD varies depending on HbA1c levels, with a prevalence of 0.463% in patients having levels within the diabetic range, while lower prevalences of 0.392% and 0.416% are found in patients with prediabetes or uncontrolled diabetes, respectively. Conclusions: This study provides further insight into the relationship between DM and DD. These findings may be attributed to the increased accumulation of advanced glycosylated end products (AGEs) in patients with diabetes. Future research exploring the connection between AGE accumulation and DD development may enhance our understanding of the relationship between DD and DM.
Language	English
Publishing date	2023-07-13
Publishing country	England
Document type	Journal Article
ISSN	2730-664X
ISSN (online)	2730-664X
DOI	10.1038/s43856-023-00332-7
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Host cell depletion of tryptophan by IFNγ-induced Indoleamine 2,3-dioxygenase 1 (IDO1) inhibits lysosomal replication of Coxiella burnetii.

Ganesan, Sandhya / Roy, Craig R

PLoS pathogens

2019 Volume 15, Issue 8, Page(s) e1007955

Abstract: Most intracellular pathogens that reside in a vacuole prevent transit of their compartment to lysosomal organelles. Effector mechanisms induced by the pro-inflammatory cytokine Interferon-gamma (IFNγ) can promote the delivery of pathogen-occupied ... ...

Abstract	Most intracellular pathogens that reside in a vacuole prevent transit of their compartment to lysosomal organelles. Effector mechanisms induced by the pro-inflammatory cytokine Interferon-gamma (IFNγ) can promote the delivery of pathogen-occupied vacuoles to lysosomes for proteolytic degradation and are therefore important for host defense against intracellular pathogens. The bacterial pathogen Coxiella burnetii is unique in that, transport to the lysosome is essential for replication. The bacterium modulates membrane traffic to create a specialized autophagolysosomal compartment called the Coxiella-containing vacuole (CCV). Importantly, IFNγ signaling inhibits intracellular replication of C. burnetii, raising the question of which IFNγ-activated mechanisms restrict replication of a lysosome-adapted pathogen. To address this question, siRNA was used to silence a panel of IFNγ-induced genes in HeLa cells to identify genes required for restriction of C. burnetii intracellular replication. This screen demonstrated that Indoleamine 2,3-dioxygenase 1 (IDO1) contributes to IFNγ-mediated restriction of C. burnetii. IDO1 is an enzyme that catabolizes cellular tryptophan to kynurenine metabolites thereby reducing tryptophan availability in cells. Cells deficient in IDO1 function were more permissive for C. burnetii replication when treated with IFNγ, and supplementing IFNγ-treated cells with tryptophan enhanced intracellular replication. Additionally, ectopic expression of IDO1 in host cells was sufficient to restrict replication of C. burnetii in the absence of IFNγ signaling. Using differentiated THP1 macrophage-like cells it was determined that IFNγ-activation resulted in IDO1 production, and that supplementation of IFNγ-activated THP1 cells with tryptophan enhanced C. burnetii replication. Thus, this study identifies IDO1 production as a key cell-autonomous defense mechanism that limits infection by C. burnetii, which suggests that peptides derived from hydrolysis of proteins in the CCV do not provide an adequate supply of tryptophan for bacterial replication.
MeSH term(s)	Coxiella burnetii/drug effects ; Coxiella burnetii/pathogenicity ; HeLa Cells ; Host-Pathogen Interactions ; Humans ; Indoleamine-Pyrrole 2,3,-Dioxygenase/antagonists & inhibitors ; Indoleamine-Pyrrole 2,3,-Dioxygenase/genetics ; Indoleamine-Pyrrole 2,3,-Dioxygenase/metabolism ; Interferon-gamma/pharmacology ; Lysosomes/virology ; Macrophages/metabolism ; Macrophages/pathology ; Macrophages/virology ; Q Fever/genetics ; Q Fever/prevention & control ; Q Fever/virology ; RNA, Small Interfering/genetics ; Tryptophan/metabolism ; Virus Replication/genetics
Chemical Substances	IDO1 protein, human ; Indoleamine-Pyrrole 2,3,-Dioxygenase ; RNA, Small Interfering ; Interferon-gamma (82115-62-6) ; Tryptophan (8DUH1N11BX)
Language	English
Publishing date	2019-08-28
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2205412-1
ISSN	1553-7374 ; 1553-7366
ISSN (online)	1553-7374
ISSN	1553-7366
DOI	10.1371/journal.ppat.1007955
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Syntaxin 11 Contributes to the Interferon-Inducible Restriction of Coxiella burnetii Intracellular Infection.

Ganesan, Sandhya / Alvarez, Natalie N / Steiner, Samuel / Fowler, Karen M / Corona, Abigail K / Roy, Craig R

mBio

2023 Volume 14, Issue 1, Page(s) e0354522

Abstract: There is a limited understanding of host defense mechanisms targeting intracellular pathogens that proliferate in a lysosome. Coxiella burnetii is a model bacterial pathogen capable of replicating in the hydrolytic and acidic environment of the lysosome. ...

Abstract	There is a limited understanding of host defense mechanisms targeting intracellular pathogens that proliferate in a lysosome. Coxiella burnetii is a model bacterial pathogen capable of replicating in the hydrolytic and acidic environment of the lysosome. It has been shown that gamma interferon (IFNγ)-stimulated host cells restrict C. burnetii replication by a mechanism that involves host IDO1 depletion of tryptophan. Host cells deficient in IDO1 activity, however, retain the ability to restrict C. burnetii replication when stimulated with IFNγ, which suggests additional mechanisms of host defense. This study identified syntaxin 11 (STX11) as a host protein that contributes to IFNγ-mediated suppression of C. burnetii replication. STX11 is a SNARE protein; SNARE proteins are proteins that mediate fusion of host vesicles with specific subcellular organelles. Depletion of STX11 using either small interfering RNA (siRNA)- or CRISPR-based approaches enhanced C. burnetii replication intracellularly. Stable expression of STX11 reduced C. burnetii replication in epithelial cells and macrophages, which indicates that this STX11-dependent cell-autonomous response is operational in multiple cell types and can function independently of other IFNγ-induced factors. Fluorescently tagged STX11 localized to the
MeSH term(s)	Humans ; Coxiella burnetii ; Host-Pathogen Interactions/physiology ; Interferon-gamma/metabolism ; Interferons/metabolism ; Q Fever/metabolism ; Qa-SNARE Proteins/genetics ; Qa-SNARE Proteins/metabolism ; RNA, Small Interfering/metabolism ; Vacuoles/metabolism
Chemical Substances	Interferon-gamma (82115-62-6) ; Interferons (9008-11-1) ; Qa-SNARE Proteins ; RNA, Small Interfering ; STX11 protein, human
Language	English
Publishing date	2023-02-02
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2557172-2
ISSN	2150-7511 ; 2161-2129
ISSN (online)	2150-7511
ISSN	2161-2129
DOI	10.1128/mbio.03545-22
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Measures of Bone Mineral Carbonate Content and Mineral Maturity/Crystallinity for FT-IR and Raman Spectroscopic Imaging Differentially Relate to Physical-Chemical Properties of Carbonate-Substituted Hydroxyapatite.

Taylor, Erik A / Mileti, Cassidy J / Ganesan, Sandhya / Kim, Joo Ho / Donnelly, Eve

Calcified tissue international

2021 Volume 109, Issue 1, Page(s) 77–91

Abstract: Bone mineral carbonate content assessed by vibrational spectroscopy relates to fracture incidence, and mineral maturity/ crystallinity (MMC) relates to tissue age. As FT-IR and Raman spectroscopy become more widely used to characterize the chemical ... ...

Abstract	Bone mineral carbonate content assessed by vibrational spectroscopy relates to fracture incidence, and mineral maturity/ crystallinity (MMC) relates to tissue age. As FT-IR and Raman spectroscopy become more widely used to characterize the chemical composition of bone in pre-clinical and translational studies, their bone mineral outcomes require improved validation to inform interpretation of spectroscopic data. In this study, our objectives were (1) to relate Raman and FT-IR carbonate:phosphate ratios calculated through direct integration of peaks to gold-standard analytical measures of carbonate content and underlying subband ratios; (2) to relate Raman and FT-IR MMC measures to gold-standard analytical measures of crystal size in chemical standards and native bone powders. Raman and FT-IR direct integration carbonate:phosphate ratios increased with carbonate content (Raman: p < 0.01, R
MeSH term(s)	Carbonates ; Durapatite ; Hydroxyapatites ; Spectroscopy, Fourier Transform Infrared ; Spectrum Analysis, Raman
Chemical Substances	Carbonates ; Hydroxyapatites ; Durapatite (91D9GV0Z28)
Language	English
Publishing date	2021-03-12
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	304266-2
ISSN	1432-0827 ; 0944-0747 ; 0008-0594 ; 0171-967X
ISSN (online)	1432-0827
ISSN	0944-0747 ; 0008-0594 ; 0171-967X
DOI	10.1007/s00223-021-00825-4
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 317: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Potentiation of Cytokine-Mediated Restriction of

Ngwaga, Tshegofatso / Hydock, Alex J / Ganesan, Sandhya / Shames, Stephanie R

Journal of bacteriology

2019 Volume 201, Issue 14

Abstract: Legionella ... ...

Abstract	Legionella pneumophila
MeSH term(s)	Animals ; Bacterial Proteins/physiology ; Cells, Cultured ; Cytokines/immunology ; Cytoplasm/metabolism ; Host-Pathogen Interactions ; Immunity, Innate ; Legionella/physiology ; Macrophages/immunology ; Macrophages/microbiology ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Protein Transport ; Signal Transduction
Chemical Substances	Bacterial Proteins ; Cytokines
Language	English
Publishing date	2019-06-21
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2968-3
ISSN	1098-5530 ; 0021-9193
ISSN (online)	1098-5530
ISSN	0021-9193
DOI	10.1128/JB.00755-18
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ud II Zs.50: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Peripheral cortical bone density predicts vertebral bone mineral properties in spine fusion surgery patients.

Heilbronner, Alison K / Dash, Alexander / Straight, Beth E / Snyder, Leah J / Ganesan, Sandhya / Adu, Kobby B / Jae, Andy / Clare, Shannon / Billings, Emma / Kim, Han Jo / Cunningham, Matthew / Lebl, Darren R / Donnelly, Eve / Stein, Emily M

Bone

2023 Volume 169, Page(s) 116678

Abstract: Spine fusion surgery is one of the most common orthopedic procedures, with over 400,000 performed annually to correct deformities and pain. However, complications occur in approximately one third of cases. While many of these complications may be related ...

Abstract	Spine fusion surgery is one of the most common orthopedic procedures, with over 400,000 performed annually to correct deformities and pain. However, complications occur in approximately one third of cases. While many of these complications may be related to poor bone quality, it is difficult to detect bone abnormalities prior to surgery. Areal BMD (aBMD) assessed by DXA may be artifactually high in patients with spine pathology, leading to missed diagnosis of deficits. In this study, we related preoperative imaging characteristics of both central and peripheral sites to direct measurements of bone quality in vertebral biopsies. We hypothesized that pre-operative imaging outcomes would relate to vertebral bone mineralization and collagen properties. Pre-operative assessments included DXA measurements of aBMD of the spine, hip, and forearm, central quantitative computed tomography (QCT) of volumetric BMD (vBMD) at the lumbar spine, and high resolution peripheral quantitative computed tomography (HRpQCT; Xtreme CT2) measurements of vBMD and microarchitecture at the distal radius and tibia. Bone samples were collected intraoperatively from the lumbar vertebrae and analyzed using Fourier-transform Infrared (FTIR) spectroscopy. Bone samples were obtained from 23 postmenopausal women (mean age 67 ± 7 years, BMI 28 ± 8 kg/m
MeSH term(s)	Humans ; Female ; Middle Aged ; Aged ; Absorptiometry, Photon/methods ; Bone Density ; Bone and Bones ; Cortical Bone ; Lumbar Vertebrae ; Radius
Language	English
Publishing date	2023-01-14
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	632515-4
ISSN	1873-2763 ; 8756-3282
ISSN (online)	1873-2763
ISSN	8756-3282
DOI	10.1016/j.bone.2023.116678
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1571: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 332: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: NF-κB/Rel proteins and the humoral immune responses of Drosophila melanogaster.

Ganesan, Sandhya / Aggarwal, Kamna / Paquette, Nicholas / Silverman, Neal

Current topics in microbiology and immunology

2010 Volume 349, Page(s) 25–60

Abstract: Nuclear Factor-κB (NF-κB)/Rel transcription factors form an integral part of innate immune defenses and are conserved throughout the animal kingdom. Studying the function, mechanism of activation and regulation of these factors is crucial for ... ...

Abstract	Nuclear Factor-κB (NF-κB)/Rel transcription factors form an integral part of innate immune defenses and are conserved throughout the animal kingdom. Studying the function, mechanism of activation and regulation of these factors is crucial for understanding host responses to microbial infections. The fruit fly Drosophila melanogaster has proved to be a valuable model system to study these evolutionarily conserved NF-κB mediated immune responses. Drosophila combats pathogens through humoral and cellular immune responses. These humoral responses are well characterized and are marked by the robust production of a battery of anti-microbial peptides. Two NF-κB signaling pathways, the Toll and the IMD pathways, are responsible for the induction of these antimicrobial peptides. Signal transduction in these pathways is strikingly similar to that in mammalian TLR pathways. In this chapter, we discuss in detail the molecular mechanisms of microbial recognition, signal transduction and NF-κB regulation, in both the Toll and the IMD pathways. Similarities and differences relative to their mammalian counterparts are discussed, and recent advances in our understanding of the intricate regulatory networks in these NF-κB signaling pathways are also highlighted.
MeSH term(s)	Adaptive Immunity ; Animals ; Drosophila Proteins/physiology ; Drosophila melanogaster/immunology ; Immunity, Humoral ; NF-kappa B/physiology ; Signal Transduction ; Toll-Like Receptors/physiology ; Transcription Factors/physiology
Chemical Substances	Drosophila Proteins ; NF-kappa B ; Rel protein, Drosophila ; Tl protein, Drosophila ; Toll-Like Receptors ; Transcription Factors ; imd protein, Drosophila
Language	English
Publishing date	2010-09-18
Publishing country	Germany
Document type	Journal Article ; Review
ISSN	0070-217X
ISSN	0070-217X
DOI	10.1007/82_2010_107
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Bonn / Germany

Einzelsignatur: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article: 87 : Caspase-8 regulates β-glucan induced IL-1β production and cell death

Ganesan, Sandhya / K. Rathinam, Vijay A / Bossaller, Lukas / Kaiser, William J / Mocarski, Edward S / Green, Douglas R / Silverman, Neal S / Fitzgerald, Katherine A

Cytokine. 2013 Sept., v. 63, no. 3

2013

Abstract: Inflammasomes play a crucial role in host defense against a variety of microbial infections through regulating the maturation of the IL-1 family of cytokines as well as pyroptotic cell death. We have previously identified an essential role for the NLRP3- ... ...

Abstract	Inflammasomes play a crucial role in host defense against a variety of microbial infections through regulating the maturation of the IL-1 family of cytokines as well as pyroptotic cell death. We have previously identified an essential role for the NLRP3-ASC-Caspase-1 inflammasome in IL-1β production triggered by the fungal pathogen, C. albicans and the importance of this pathway in the clearance of C. albicans in an in vivo mouse model of Oropharyngeal Candidiasis (OPC). Here, we uncovered an important role for the pro-apoptotic caspase, Caspase-8 in IL-1β production induced by β-glucan, a potent immunostimulatory component of fungal cell walls. We found that β-glucan induced IL-1β production requires Caspase-8, in addition to the NLRP3-ASC-Caspase-1 inflammasome. We treated bone marrow derived macrophages and dendritic cells from RIP3⁻/⁻Caspase-8⁻/⁻ mice with various β-glucan related molecules, such as curdlan, fungal whole glucan particles or heat killed C. albicans, and found that Caspase-8 was required for IL-1β production with these stimuli but not with canonical inflammasome activators such as silica, nigericin or poly (dA:dT). Of note, Caspase-8 deficiency did not affect β-glucan induced synthesis of pro-IL1β. In addition, RIP3⁻/⁻Caspase-8⁻/⁻cells were protected from β-glucan induced cell death, whereas RIP3⁻/⁻ deficiency did not affect β-glucan induced IL-1β production or cell death. In contrast to these observations, we found that IL-1β production and cell death in response to live C. albicans infection was not Caspase-8 dependent, raising the possibility that β-glucan may not be exposed by C. albicans to engage this pathway. This study establishes a key link between Caspase-8 and Caspase-1 in coordinating cytokine secretion and cell death in response to fungal pathogenic patterns, and provides evidence for how pathways leading to inflammation and cell death are intertwined for an effective host immune response.
Keywords	animal models ; apoptosis ; bone marrow ; candidiasis ; caspase-1 ; caspase-8 ; cell walls ; curdlan ; dendritic cells ; fungi ; heat ; immune response ; inflammation ; interleukin-1 ; macrophages ; mice ; nigericin ; pathogens ; secretion ; silica
Language	English
Dates of publication	2013-09
Size	p. 263-264.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	1018055-2
ISSN	1096-0023 ; 1043-4666
ISSN (online)	1096-0023
ISSN	1043-4666
DOI	10.1016/j.cyto.2013.06.087
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.A 2840: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: A novel role for the NLRC4 inflammasome in mucosal defenses against the fungal pathogen Candida albicans.

Tomalka, Jeffrey / Ganesan, Sandhya / Azodi, Elaheh / Patel, Krupen / Majmudar, Parth / Hall, Brian A / Fitzgerald, Katherine A / Hise, Amy G

PLoS pathogens

2011 Volume 7, Issue 12, Page(s) e1002379

Abstract: Candida sp. are opportunistic fungal pathogens that colonize the skin and oral cavity and, when overgrown under permissive conditions, cause inflammation and disease. Previously, we identified a central role for the NLRP3 inflammasome in regulating IL-1β ...

Abstract	Candida sp. are opportunistic fungal pathogens that colonize the skin and oral cavity and, when overgrown under permissive conditions, cause inflammation and disease. Previously, we identified a central role for the NLRP3 inflammasome in regulating IL-1β production and resistance to dissemination from oral infection with Candida albicans. Here we show that mucosal expression of NLRP3 and NLRC4 is induced by Candida infection, and up-regulation of these molecules is impaired in NLRP3 and NLRC4 deficient mice. Additionally, we reveal a role for the NLRC4 inflammasome in anti-fungal defenses. NLRC4 is important for control of mucosal Candida infection and impacts inflammatory cell recruitment to infected tissues, as well as protects against systemic dissemination of infection. Deficiency in either NLRC4 or NLRP3 results in severely attenuated pro-inflammatory and antimicrobial peptide responses in the oral cavity. Using bone marrow chimeric mouse models, we show that, in contrast to NLRP3 which limits the severity of infection when present in either the hematopoietic or stromal compartments, NLRC4 plays an important role in limiting mucosal candidiasis when functioning at the level of the mucosal stroma. Collectively, these studies reveal the tissue specific roles of the NLRP3 and NLRC4 inflammasome in innate immune responses against mucosal Candida infection.
MeSH term(s)	Animals ; Apoptosis Regulatory Proteins/immunology ; Apoptosis Regulatory Proteins/metabolism ; Calcium-Binding Proteins/immunology ; Calcium-Binding Proteins/metabolism ; Candida albicans/immunology ; Candidiasis/immunology ; Candidiasis/metabolism ; Carrier Proteins/immunology ; Carrier Proteins/metabolism ; Enzyme-Linked Immunosorbent Assay ; Fluorescent Antibody Technique ; Immunity, Innate/immunology ; Immunity, Mucosal ; Inflammasomes/immunology ; Inflammasomes/metabolism ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Mouth Mucosa/immunology ; Mouth Mucosa/metabolism ; Mouth Mucosa/microbiology ; NLR Family, Pyrin Domain-Containing 3 Protein ; Real-Time Polymerase Chain Reaction
Chemical Substances	Apoptosis Regulatory Proteins ; Calcium-Binding Proteins ; Carrier Proteins ; Inflammasomes ; Ipaf protein, mouse ; NLR Family, Pyrin Domain-Containing 3 Protein ; Nlrp3 protein, mouse
Language	English
Publishing date	2011-12-08
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2205412-1
ISSN	1553-7374 ; 1553-7366
ISSN (online)	1553-7374
ISSN	1553-7366
DOI	10.1371/journal.ppat.1002379
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Caspase-8 modulates dectin-1 and complement receptor 3-driven IL-1β production in response to β-glucans and the fungal pathogen, Candida albicans.

Ganesan, Sandhya / Rathinam, Vijay A K / Bossaller, Lukas / Army, Kelly / Kaiser, William J / Mocarski, Edward S / Dillon, Christopher P / Green, Douglas R / Mayadas, Tanya N / Levitz, Stuart M / Hise, Amy G / Silverman, Neal / Fitzgerald, Katherine A

Journal of immunology (Baltimore, Md. : 1950)

2014 Volume 193, Issue 5, Page(s) 2519–2530

Abstract: Inflammasomes are central mediators of host defense to a wide range of microbial pathogens. The nucleotide-binding domain and leucine-rich repeat containing family (NLR), pyrin domain-containing 3 (NLRP3) inflammasome plays a key role in triggering ... ...

Abstract	Inflammasomes are central mediators of host defense to a wide range of microbial pathogens. The nucleotide-binding domain and leucine-rich repeat containing family (NLR), pyrin domain-containing 3 (NLRP3) inflammasome plays a key role in triggering caspase-1-dependent IL-1β maturation and resistance to fungal dissemination in Candida albicans infection. β-Glucans are major components of fungal cell walls that trigger IL-1β secretion in both murine and human immune cells. In this study, we sought to determine the contribution of β-glucans to C. albicans-induced inflammasome responses in mouse dendritic cells. We show that the NLRP3-apoptosis-associated speck-like protein containing caspase recruitment domain protein-caspase-1 inflammasome is absolutely critical for IL-1β production in response to β-glucans. Interestingly, we also found that both complement receptor 3 (CR3) and dectin-1 play a crucial role in coordinating β-glucan-induced IL-1β processing as well as a cell death response. In addition to the essential role of caspase-1, we identify an important role for the proapoptotic protease caspase-8 in promoting β-glucan-induced cell death and NLRP3 inflammasome-dependent IL-1β maturation. A strong requirement for CR3 and caspase-8 also was found for NLRP3-dependent IL-1β production in response to heat-killed C. albicans. Taken together, these results define the importance of dectin-1, CR3, and caspase-8, in addition to the canonical NLRP3 inflammasome, in mediating β-glucan- and C. albicans-induced innate responses in dendritic cells. Collectively, these findings establish a novel link between β-glucan recognition receptors and the inflammatory proteases caspase-8 and caspase-1 in coordinating cytokine secretion and cell death in response to immunostimulatory fungal components.
MeSH term(s)	Animals ; Candida albicans/genetics ; Candida albicans/immunology ; Candidiasis/genetics ; Candidiasis/immunology ; Candidiasis/pathology ; Carrier Proteins/genetics ; Carrier Proteins/immunology ; Caspase 8/genetics ; Caspase 8/immunology ; Cell Death/genetics ; Cell Death/immunology ; Dendritic Cells/immunology ; Dendritic Cells/pathology ; Fungal Polysaccharides/genetics ; Fungal Polysaccharides/immunology ; Humans ; Interleukin-1beta/genetics ; Interleukin-1beta/immunology ; Lectins, C-Type/genetics ; Lectins, C-Type/immunology ; Macrophage-1 Antigen/genetics ; Macrophage-1 Antigen/immunology ; Mice ; Mice, Knockout ; NLR Family, Pyrin Domain-Containing 3 Protein ; beta-Glucans/immunology
Chemical Substances	Carrier Proteins ; Fungal Polysaccharides ; IL1B protein, mouse ; Interleukin-1beta ; Lectins, C-Type ; Macrophage-1 Antigen ; NLR Family, Pyrin Domain-Containing 3 Protein ; Nlrp3 protein, mouse ; beta-Glucans ; dectin 1 ; Casp8 protein, mouse (EC 3.4.22.-) ; Caspase 8 (EC 3.4.22.-)
Language	English
Publishing date	2014-07-25
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	3056-9
ISSN	1550-6606 ; 0022-1767 ; 1048-3233 ; 1047-7381
ISSN (online)	1550-6606
ISSN	0022-1767 ; 1048-3233 ; 1047-7381
DOI	10.4049/jimmunol.1400276
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ud II Zs.37: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MG 28: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito