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  1. Article: Impact of soil inorganic nitrogen on bacterial phylogeny in estuarine intertidal zones: a study of nitrogen metabolism.

    Li, Siqi / Liu, Tianyang / Liu, Cheng / Sun, Donglei / Yan, Qin / Gao, Dengzhou / Zhang, Zongxiao

    Frontiers in microbiology

    2024  Volume 14, Page(s) 1341564

    Abstract: Here we investigated the potential impacts of soil inorganic nitrogen (SIN) content on the phylogenetic characteristics and ecological functions of soil bacterial communities in estuarine intertidal zones in China, aiming to comprehend the response ... ...

    Abstract Here we investigated the potential impacts of soil inorganic nitrogen (SIN) content on the phylogenetic characteristics and ecological functions of soil bacterial communities in estuarine intertidal zones in China, aiming to comprehend the response mechanism of soil microorganisms to variations in SIN content within estuarine wetlands. Our results show that SIN in estuarine areas has a significant spatiotemporal variation on spatial and seasonal scales, in this study and is significantly associated with the phylogenetic diversity and phylogenetic turnover of soil bacterial communities. In addition, the results of the metagenomic analysis showed that the relative abundance of nitrogen-cycling functional genes in bacterial communities did not differ significantly in sampling sites and seasons, and weakly correlated with SIN content. Further, the results based on structural equation modeling (SEM) analysis showed that SIN directly and significantly regulated the phylogenetic characteristics of bacterial communities, thereby indirectly affecting the potential of bacterial nitrogen metabolism. This study emphasizes the key influence of SIN variations on the phylogenetic dissimilarity in soil bacterial communities. Moreover, although there was a weak direct relationship between the functional characteristics of the bacterial nitrogen metabolism and SIN content, the spatiotemporal variation of bacterial nitrogen metabolic potential may be indirectly regulated by SIN content by influencing the phylogenetic diversity in bacterial communities. Our study unravels the pivotal mechanisms through which SIN content influences bacterial communities, thereby offering novel insights into the microbial intricacies governing nitrogen metabolism within estuaries.
    Language English
    Publishing date 2024-01-05
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2023.1341564
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Editorial: Linking microbial-driven key processes with carbon and nitrogen cycling in estuarine, coastal, and the nearshore areas.

    Lin, Genmei / Gao, Dengzhou / Yang, Ping / Liu, Shuting / Sun, Dongyao / Lin, Xianbiao

    Frontiers in microbiology

    2024  Volume 15, Page(s) 1382148

    Language English
    Publishing date 2024-03-18
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2024.1382148
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  3. Article: Crab bioturbation affects competition between microbial nitrogen removal and retention in estuarine and coastal wetlands

    Liu, Cheng / Xia, Jiangbao / Cui, Qian / Zhang, Dongjie / Liu, Min / Hou, Lijun / Gao, Dengzhou

    Environmental research. 2022 Dec., v. 215

    2022  

    Abstract: As the important benthic animal in coastal wetlands, crab bioturbation may significantly affect the nitrogen (N) budgets by regulating microbial N transformation processes. However, the response of interaction between different microbial N processes to ... ...

    Abstract As the important benthic animal in coastal wetlands, crab bioturbation may significantly affect the nitrogen (N) budgets by regulating microbial N transformation processes. However, the response of interaction between different microbial N processes to crab bioturbation remains poorly understood. Here, a 30-day microcosmic experiment was conducted using sediment collected from the Yangtze Estuary wetland, followed by the determination of temporal variations of physicochemical parameters, N removal (denitrification plus anammox, which is defined as N₂ production) and retention rates (nitrate dissimilatory reduction to ammonium, DNRA) as well as relevant gene abundances in response to different crabs densities. The results showed that crab bioturbation simultaneously promoted the rates of N₂ production and DNRA processes. These two process rates were positively associated with the intensity of crab bioturbation, which was supported by molecular analysis of relevant functional gene abundance. Crab bioturbation was more beneficial to DNRA than N₂ production. Due to this disproportionate stimulation, crab bioturbation increased the importance of DNRA, indicating that N retention was becoming more significant under crab bioturbation in estuarine and coastal wetlands. The variations of sediment total organic carbon and oxygen availability driven by crab bioturbation were the critical factors mediating the changes in microbial N removal and retention. Overall, our findings highlighted that crab bioturbation can affect the N budgets in estuarine and coastal wetlands by altering the competition between microbial N removal and retention.
    Keywords ammonium ; anaerobic ammonium oxidation ; bioturbation ; crabs ; denitrification ; estuaries ; genes ; microbial nitrogen ; nitrates ; oxygen ; research ; sediments ; total organic carbon ; wetlands
    Language English
    Dates of publication 2022-12
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 205699-9
    ISSN 1096-0953 ; 0013-9351
    ISSN (online) 1096-0953
    ISSN 0013-9351
    DOI 10.1016/j.envres.2022.114280
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  4. Article: Switches of methane production pathways and emissions with human activity intensity in subtropical estuaries

    Li, Xiaofei / Qi, Mengting / Gao, Dengzhou / Liu, Min / Hou, Lijun

    Journal of hydrology. 2022 Sept., v. 612

    2022  

    Abstract: Nutrient loading caused by human activities can drive changes in methane (CH₄) production pathways, yet the mechanisms and magnitudes of estuary CH₄ emissions remain poorly understood. Here we investigated CH₄ fluxes and δ¹³C-CH₄ values in wet and dry ... ...

    Abstract Nutrient loading caused by human activities can drive changes in methane (CH₄) production pathways, yet the mechanisms and magnitudes of estuary CH₄ emissions remain poorly understood. Here we investigated CH₄ fluxes and δ¹³C-CH₄ values in wet and dry seasons from five subtropical estuaries with varying human activity intensity and hydrologic conditions. Water dissolved CH₄ concentrations varied from 0.19 to 0.51 µmol CH₄ L–¹ in these studied estuaries, resulting in coincident water–air CH₄ fluxes ranging from 5.46 to 29.6 µg CH₄ m–²h⁻¹. Water CH₄ concentrations and fluxes were significantly lower in the wet than dry season, and were higher in the high human-impacted estuaries, indicating that large discharge and water flow could slow down areal CH₄ emissions. The ratios of C-CH₄ to organic carbon (OC) were much higher and showed a positive response to increasing nitrogen concentrations in high human-impacted estuaries, suggesting that eutrophic estuaries trigger high CH₄ emissions. In addition, an increase in δ¹³C-CH₄ and a declining in fractionation factor (αₐₚₚ) from the low to high human-impacted estuaries were accompanied by a switch of CH₄ production from hydrogenotrophic to acetoclastic pathways. These results suggest that human activity intensity can drive the alterations in the pathways and magnitudes of CH₄ production and that hydrologic conditions can modify CH₄ emissions, which have important implications for estimates of CH₄ emissions from global estuaries under increasing human activity intensity.
    Keywords dry season ; estuaries ; eutrophication ; fractionation ; humans ; methane ; methane production ; nitrogen ; organic carbon ; water flow
    Language English
    Dates of publication 2022-09
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1473173-3
    ISSN 1879-2707 ; 0022-1694
    ISSN (online) 1879-2707
    ISSN 0022-1694
    DOI 10.1016/j.jhydrol.2022.128061
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  5. Article: N2O emission dynamics along an intertidal elevation gradient in a subtropical estuary: Importance of N2O consumption

    Gao, Dengzhou / Hou, Lijun / Liu, Min / Zheng, Yanling / Yin, Guoyu / Niu, Yuhui

    Environmental research. 2022 Apr. 01, v. 205

    2022  

    Abstract: Studying nitrous oxide (N₂O) production and consumption processes along an intertidal elevation gradient can improve the understanding of N₂O dynamics among coastal wetlands. A natural-abundance isotope technique was applied to characterize the processes ...

    Abstract Studying nitrous oxide (N₂O) production and consumption processes along an intertidal elevation gradient can improve the understanding of N₂O dynamics among coastal wetlands. A natural-abundance isotope technique was applied to characterize the processes responsible for N₂O emission in high, middle and low intertidal zones in the Yangtze Estuary. The results showed that N₂O emission rates in high tidal zones (0.84 ± 0.35 nmol g⁻¹ h⁻¹) were significantly higher than those in middle (0.21 ± 0.04 nmol g⁻¹ h⁻¹) and low tidal zones (0.26 ± 0.05 nmol g⁻¹ h⁻¹). Gross N₂O production and consumption rates were greater in high and low tidal zones than in middle tidal zones, whereas N₂O consumption proportions generally increased from high to low tidal zones. N₂O consumption was quite pronounced, implying that N₂O emission in estuarine wetlands accounts for only a small fraction of the total production. Higher degrees of N₂O consumption were the pivotal driver of less N₂O emission in low tidal zones. Bacterial denitrification (>84%) was the dominant pathway, although hydroxylamine (NH₂OH) oxidation/fungal denitrification contributed substantially to N₂O production in high tidal flats. The contribution to N₂O production exhibited a decrease in NH₂OH oxidation/fungal denitrification and an increase in bacterial denitrification with decreasing elevation. Changes in N₂O dynamics along the elevation gradient were affected by carbon and nitrogen substrate availabilities as well as the redox environments. Overall, our findings highlight the importance of N₂O consumption in controlling N₂O emission in intertidal wetlands, especially with higher inundation frequencies and durations.
    Keywords altitude ; carbon ; denitrification ; estuaries ; fungi ; hydroxylamine ; isotopes ; littoral zone ; nitrogen ; nitrous oxide ; nitrous oxide production ; oxidation ; research
    Language English
    Dates of publication 2022-0401
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 205699-9
    ISSN 1096-0953 ; 0013-9351
    ISSN (online) 1096-0953
    ISSN 0013-9351
    DOI 10.1016/j.envres.2021.112432
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  6. Article: Nitrous oxide emissions from subtropical estuaries: Insights for environmental controls and implications

    Li, Xiaofei / Qi, Mengting / Gao, Dengzhou / Liu, Min / Sardans, Jordi / Peñuelas, Josep / Hou, Lijun

    Water research. 2022 Apr. 01, v. 212

    2022  

    Abstract: Estuaries are expected to contribute large nitrous oxide (N₂O) emissions, however the environmental controls and implications of N₂O emissions have not been well understood. Here we investigated water N₂O concentrations, fluxes and sources in wet and dry ...

    Abstract Estuaries are expected to contribute large nitrous oxide (N₂O) emissions, however the environmental controls and implications of N₂O emissions have not been well understood. Here we investigated water N₂O concentrations, fluxes and sources in wet and dry seasons for 2019–2020 in five subtropical estuaries spanning hydrologic characteristics and nitrogen concentrations gradient. Water dissolved N₂O concentrations and fluxes were in a range of 15.8–84.9 nmol L–¹ and 0.66–22.2 µg m–² h–¹, respectively. These studied estuaries were oversaturated in N₂O, with the saturations of 118–615%. Water dissolved N₂O concentrations, saturations and fluxes increased significantly as nitrogen concentrations increase, whereas they did not differ significantly between the wet and dry seasons. Water N₂O emissions, however, were also lower in the estuaries characterized by large discharge and water flow. N₂O saturations and fluxes were determined directly by water nitrogen and oxygen concentrations and more indirectly by water temperature and velocity. The δ¹⁵N-N₂O and site preference-N₂O varied respectively from 2.86 to 11.31‰ and from 1.58 to 11.72‰, which overlapped the values between nitrification and denitrification. Nitrification and denitrification were responsible for 18.7–38.1% and 61.9–81.3% of N₂O emissions, respectively. Indirect N₂O emission factors were 0.08–0.14% and decreased with increasing total nitrogen concentrations. It is estimated that water N₂O emissions in CO₂ equiv could offset approximately 4.9% of average CO₂ sink of China estuaries. Therefore, these results suggest that nitrogen concentrations and hydrologic characteristics together modify N₂O emissions and that estuaries may be the important contributors to N₂O emissions.
    Keywords carbon dioxide ; denitrification ; nitrification ; nitrogen ; nitrous oxide ; oxygen ; research ; total nitrogen ; water ; water flow ; water temperature ; China
    Language English
    Dates of publication 2022-0401
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 202613-2
    ISSN 1879-2448 ; 0043-1354
    ISSN (online) 1879-2448
    ISSN 0043-1354
    DOI 10.1016/j.watres.2022.118110
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  7. Article ; Online: Anaerobic oxidation of methane with denitrification in sediments of a subtropical estuary: Rates, controlling factors and environmental implications.

    Li, Xiaofei / Lai, Derrick Y F / Gao, Dengzhou

    Journal of environmental management

    2020  Volume 273, Page(s) 111151

    Abstract: Anaerobic oxidation of methane with denitrification (DAMO), as an important microbial process regulating methane emission, has been widely reported in freshwater ecosystems. However, the DAMO process and associated biogeochemical controls in estuaries ... ...

    Abstract Anaerobic oxidation of methane with denitrification (DAMO), as an important microbial process regulating methane emission, has been widely reported in freshwater ecosystems. However, the DAMO process and associated biogeochemical controls in estuaries remain poorly understood. Here, we used
    MeSH term(s) Anaerobiosis ; Denitrification ; Ecosystem ; Estuaries ; Geologic Sediments ; Methane ; Nitrites ; Oxidation-Reduction ; RNA, Ribosomal, 16S
    Chemical Substances Nitrites ; RNA, Ribosomal, 16S ; Methane (OP0UW79H66)
    Language English
    Publishing date 2020-08-03
    Publishing country England
    Document type Journal Article
    ZDB-ID 184882-3
    ISSN 1095-8630 ; 0301-4797
    ISSN (online) 1095-8630
    ISSN 0301-4797
    DOI 10.1016/j.jenvman.2020.111151
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  8. Article ; Online: Sediment nitrate reduction processes in response to environmental gradients along an urban river-estuary-sea continuum.

    Wei, Hengchen / Gao, Dengzhou / Liu, Yong / Lin, Xianbiao

    The Science of the total environment

    2020  Volume 718, Page(s) 137185

    Abstract: Sediment denitrification (DEN), anaerobic ammonium oxidation (Anammox), and dissimilatory nitrate reduction to ammonium (DNRA) are three important nitrate ( ... ...

    Abstract Sediment denitrification (DEN), anaerobic ammonium oxidation (Anammox), and dissimilatory nitrate reduction to ammonium (DNRA) are three important nitrate (NO
    Language English
    Publishing date 2020-02-08
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2020.137185
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  9. Article: Sediment nitrate reduction processes in response to environmental gradients along an urban river-estuary-sea continuum

    Wei, Hengchen / Gao, Dengzhou / Liu, Yong / Lin, Xianbiao

    Science of the total environment. 2020 May 20, v. 718

    2020  

    Abstract: Sediment denitrification (DEN), anaerobic ammonium oxidation (Anammox), and dissimilatory nitrate reduction to ammonium (DNRA) are three important nitrate (NO3−) reduction pathways in aquatic ecosystems. These processes modify nitrogen (N) loadings from ... ...

    Abstract Sediment denitrification (DEN), anaerobic ammonium oxidation (Anammox), and dissimilatory nitrate reduction to ammonium (DNRA) are three important nitrate (NO3−) reduction pathways in aquatic ecosystems. These processes modify nitrogen (N) loadings from land to the ocean, with important implications on the management of coastal eutrophication. While NO3− reduction has been studied intensively for various types of habitats, studies on its distributions along river-estuary-sea continua remain scarce. In this study, we examined these three pathways along a N-laden urban river-estuary-sea continuum comprised of three types of habitats (urban river, estuary, and adjacent sea) in the densely populated Shanghai-East China Sea area. The potential DEN, Anammox, and DNRA rates decreased seaward both in summer and winter in response to decreasing sediment organic matter (OM, 20 to 7 to 7 mg C g−1), ferrous oxide (9 to 2.7 to 2.8 mg Fe g−1), and bottom water dissolved inorganic nitrogen (543 to 112 to 21 μM). Among these pathways, DEN remained a major component (~69.6%) across habitats, while Anammox (47.9%) rivaled DEN (48.3%) in the urban river in winter. N retention index (NIRI), the ratio between retained and removed NO3−, ranged from 0 to 0.5 and increased downstream. Together, these results suggest that the decreasing gradients of OM and inorganic matter shape the distribution of NO3− reduction along the continuum, reflecting the diminishing impact of the river and human inputs from the urban river to the ocean. Our results highlight the importance of taking a continuum perspective in N cycling studies and emphasize the role of urban rivers as N removal hotspots, which should be a focus of research and management.
    Keywords ammonium ; anaerobic ammonium oxidation ; aquatic ecosystems ; denitrification ; dissolved inorganic nitrogen ; estuaries ; eutrophication ; ferrous oxide ; habitats ; inorganic matter ; nitrate reduction ; nitrates ; nitrogen ; nitrogen cycle ; organic matter ; pollution load ; rivers ; sediments ; summer ; winter ; China
    Language English
    Dates of publication 2020-0520
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2020.137185
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  10. Article: Anaerobic oxidation of methane with denitrification in sediments of a subtropical estuary: Rates, controlling factors and environmental implications

    Li, Xiaofei / Lai, Derrick Y.F / Gao, Dengzhou

    Journal of environmental management. 2020 Nov. 01, v. 273

    2020  

    Abstract: Anaerobic oxidation of methane with denitrification (DAMO), as an important microbial process regulating methane emission, has been widely reported in freshwater ecosystems. However, the DAMO process and associated biogeochemical controls in estuaries ... ...

    Abstract Anaerobic oxidation of methane with denitrification (DAMO), as an important microbial process regulating methane emission, has been widely reported in freshwater ecosystems. However, the DAMO process and associated biogeochemical controls in estuaries remain poorly understood. Here, we used ¹³C- and ¹⁵N-labelling experiments to quantify the potential rates of DAMO and determined the crucial factors controlling the DAMO rates in the sediment of Yangtze Estuary. Potential rates of DAMO varied greatly across the estuary, ranging from 0.07 to 0.28 nmol CO₂ g⁻¹ d⁻¹. Salinity negatively affected the DAMO and also showed an indirectly negative influence on DAMO process by high salinity inhibition on NO₃⁻ availability and denitrification. Nitrate concentrations were significantly correlated with the DAMO rates. Denitrification rates showed positive correlation with DAMO rates, implying that nitrate reduction drives the DAMO process. Sediment total organic carbon and NH₄⁺ had important effects on DAMO rates. These results together indicate that DAMO process can occur and the DAMO rates were mainly controlled by sediment NO₃⁻ and denitrification in estuary. We further conclude that increasing NO₃⁻ load can drive the DAMO process with more important implications on methane sink in estuarine ecosystems.
    Keywords carbon dioxide ; denitrification ; environmental management ; estuaries ; freshwater ; methane ; methane production ; nitrate reduction ; nitrates ; oxidation ; salinity ; sediments ; total organic carbon
    Language English
    Dates of publication 2020-1101
    Publishing place Elsevier Ltd
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 184882-3
    ISSN 1095-8630 ; 0301-4797
    ISSN (online) 1095-8630
    ISSN 0301-4797
    DOI 10.1016/j.jenvman.2020.111151
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