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Article ; Online: Mechanism of Chinese sturgeon IFN-γ inhibition on Mycobacterium marinum (Acipenser sinensis).

Yu, Ting / Xie, Meng / Luo, Kai / Zhang, Xiao / Gao, Weihua / Xu, Qiaoqing / Zhang, Shuhuan

2024 Volume 147, Page(s) 109436

Abstract: IFN-γ plays a crucial role in both innate and adaptive immune responses and is a typical Th1 cytokine that promotes Th1 response and activates macrophages. When macrophages were incubated with IFN-γ, their phagocytosis ratio against Mycobacterium marinum ...

Abstract	IFN-γ plays a crucial role in both innate and adaptive immune responses and is a typical Th1 cytokine that promotes Th1 response and activates macrophages. When macrophages were incubated with IFN-γ, their phagocytosis ratio against Mycobacterium marinum increased significantly, as observed under fluorescence microscopy. The macrophages engulfed a large number of M. marinum. The proliferative ability of macrophages treated with IFN-γ was significantly weaker on the 4th and 7th day after phagocytosis and subsequent re-infection with marine chlamydia (P < 0.001). This suggests that IFN-γ enhances the phagocytosis and killing ability of macrophages against M. marinum. IFN-γ protein also significantly increased the production of reactive oxygen species (H
MeSH term(s)	Animals ; Toll-Like Receptor 2/genetics ; Mycobacterium marinum ; Hydrogen Peroxide/metabolism ; Macrophages ; Cytokines/metabolism
Chemical Substances	Toll-Like Receptor 2 ; Hydrogen Peroxide (BBX060AN9V) ; Cytokines
Language	English
Publishing date	2024-02-17
Publishing country	England
Document type	Journal Article
ZDB-ID	1067738-0
ISSN	1095-9947 ; 1050-4648
ISSN (online)	1095-9947
ISSN	1050-4648
DOI	10.1016/j.fsi.2024.109436
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Article ; Online: ATM orchestrates ferritinophagy and ferroptosis by phosphorylating NCOA4.

Wu, Hao / Liu, Qian / Shan, Xinyi / Gao, Weihua / Chen, Quan

Autophagy

2023 Volume 19, Issue 7, Page(s) 2062–2077

Abstract: Ferroptosis is a newly characterized form of programmed cell death, which is driven by the lethal accumulation of lipid peroxides catalyzed by the intracellular bioactive iron. Targeted induction of ferroptotic cell death holds great promise for ... ...

Abstract	Ferroptosis is a newly characterized form of programmed cell death, which is driven by the lethal accumulation of lipid peroxides catalyzed by the intracellular bioactive iron. Targeted induction of ferroptotic cell death holds great promise for therapeutic design against other therapy-resistant cancers. To date, multiple post-translational modifications have been elucidated to impinge on the ferroptotic sensitivity. Here we report that the Ser/Thr protein kinase ATM, the major sensor of DNA double-strand break damage, is indispensable for ferroptosis execution. Pharmacological inhibition or genetic ablation of ATM significantly antagonizes ferroptosis. Besides, ATM ablation-induced ferroptotic resistance is largely independent of its downstream target TRP53, as cells defective in both
MeSH term(s)	Animals ; Mice ; Ferroptosis ; Autophagy ; Fibroblasts ; Transcription Factors ; Ferritins ; Iron ; Buthionine Sulfoximine
Chemical Substances	Transcription Factors ; Ferritins (9007-73-2) ; Iron (E1UOL152H7) ; pifithrin (D213B92S1Y) ; Buthionine Sulfoximine (5072-26-4)
Language	English
Publishing date	2023-02-08
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2454135-7
ISSN	1554-8635 ; 1554-8627
ISSN (online)	1554-8635
ISSN	1554-8627
DOI	10.1080/15548627.2023.2170960
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Article ; Online: Tumor Targeted Cuprous-Based Nanocomposite as Responsive Cascade Nanocatalyst for Efficient Tumor Synergistic Therapy.

Gao, Weihua / Zhang, Jie / Ding, Lina / Chang, Yi / Gao, Fangli / Yang, Pengfei / Ma, Xiaoming / Guo, Yuming

Chemistry (Weinheim an der Bergstrasse, Germany)

2024 Volume 30, Issue 10, Page(s) e202302961

Abstract: The single-functionality of traditional chemodynamic therapy (CDT) reagents usually limits the therapeutic efficacy of cancer treatment. Synergistic nanocomposites that involve cascade reaction provide a promising strategy to achieve satisfactory ... ...

Abstract	The single-functionality of traditional chemodynamic therapy (CDT) reagents usually limits the therapeutic efficacy of cancer treatment. Synergistic nanocomposites that involve cascade reaction provide a promising strategy to achieve satisfactory anticancer effects. Herein, a cuprous-based nanocomposite (CCS@GOx@HA) is fabricated, which owns the tumor targeting ability and can undergo tumor microenvironment responsive cascade reaction to enhance the tumor therapeutic efficiency significantly. Surface modification of nanocomposite with hyaluronic acid enables the targeted delivery of the nanocomposite to cancer cells. Acid-triggered decomposition of nanocomposite in cancer cell results in the release of Cu
MeSH term(s)	Humans ; Neoplasms/drug therapy ; Catalysis ; Glucose ; Hyaluronic Acid ; Nanocomposites/therapeutic use ; Hydrogen Peroxide ; Cell Line, Tumor ; Tumor Microenvironment
Chemical Substances	Glucose (IY9XDZ35W2) ; Hyaluronic Acid (9004-61-9) ; Hydrogen Peroxide (BBX060AN9V)
Language	English
Publishing date	2024-01-04
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1478547-X
ISSN	1521-3765 ; 0947-6539
ISSN (online)	1521-3765
ISSN	0947-6539
DOI	10.1002/chem.202302961
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Article ; Online: Cardiovascular outcomes by time-varying New York Heart Association class among patients with obstructive hypertrophic cardiomyopathy: a retrospective cohort study.

Wang, Yan / Gao, Weihua / Han, Xu / Jiang, Jenny / Sandler, Belinda / Li, Xiaoyan / Zema, Carla

Journal of medical economics

2023 Volume 26, Issue 1, Page(s) 1495–1506

Abstract: Aims: Assess the relationship between New York Heart Association (NYHA) functional class and cardiovascular (CV) outcomes in obstructive hypertrophic cardiomyopathy (HCM).: Materials and methods: This retrospective cohort study used the Optum Market ... ...

Abstract	Aims: Assess the relationship between New York Heart Association (NYHA) functional class and cardiovascular (CV) outcomes in obstructive hypertrophic cardiomyopathy (HCM). Materials and methods: This retrospective cohort study used the Optum Market Clarity database with linked claims and electronic health records. Adults (aged ≥18 years) with obstructive HCM and ≥1 NYHA class assessment after first HCM diagnosis were eligible (selection period: 2007-2021). Thirteen outcomes were assessed following the index date (first documented NYHA class assessment after first HCM diagnosis in the study period): all-cause mortality; first occurrences of all-cause hospitalization; CV-related hospitalization; primary ischemic stroke or transient ischemic attack (TIA); myocardial infarction (MI); deep vein thrombosis (DVT) or pulmonary embolism (PE); and major adverse CV event (MACE); as well as first incident events of atrial fibrillation or flutter; primary ischemic stroke or TIA; heart failure; acute MI; DVT/PE; and a composite endpoint of pacemaker and cardiac resynchronization therapy. Their associations with the index NYHA class were described using the Kaplan-Meier method (mortality) or cumulative incidence functions (other outcomes). Hazard ratios between NYHA class over time and outcomes were evaluated using time-varying Cox models, adjusting for age at first observed HCM diagnosis, sex, and race. Results: Among 4,631 eligible patients, the mean age was 59 years at the first observed HCM diagnosis (female, 47%; White, 77%). The risks of all outcomes increased with worse (higher) index NYHA class and worsening NYHA class over time. Deterioration in the NYHA class from the index date was associated with increased risks of outcomes. Limitations: The study population may not be representative of all patients with obstructive HCM in the real world. Documented NYHA classes may not fully reflect the longitudinal variation of NYHA class for each patient. Conclusions: Worsening NYHA class was associated with increased risks of all-cause mortality and CV outcomes in obstructive HCM.
MeSH term(s)	Adult ; Humans ; Female ; Adolescent ; Middle Aged ; Retrospective Studies ; Ischemic Attack, Transient/epidemiology ; New York ; Cardiomyopathy, Hypertrophic/complications ; Cardiomyopathy, Hypertrophic/therapy ; Myocardial Infarction ; Ischemic Stroke
Language	English
Publishing date	2023-11-30
Publishing country	England
Document type	Journal Article
ZDB-ID	2270945-9
ISSN	1941-837X ; 1369-6998
ISSN (online)	1941-837X
ISSN	1369-6998
DOI	10.1080/13696998.2023.2277076
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Article ; Online: Emerging Pathological Engagement of Ferroptosis in Gut Diseases.

Gao, Weihua / Zhang, Ting / Wu, Hao

Oxidative medicine and cellular longevity

2021 Volume 2021, Page(s) 4246255

Abstract: Inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease, is mainly characterized by chronic and progressive inflammation that damages the gastrointestinal mucosa. Increasing studies have enlightened that dysregulated cell death ...

Abstract	Inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease, is mainly characterized by chronic and progressive inflammation that damages the gastrointestinal mucosa. Increasing studies have enlightened that dysregulated cell death occurs in the inflamed sites, leading to the disruption of the intestinal barrier and aggravating inflammatory response. Ferroptosis, a newly characterized form of regulated cell death, is driven by the lethal accumulation of lipid peroxides catalyzed by cellular free iron. It has been widely documented that the fundamental features of ferroptosis, including iron deposition, GSH exhaustion, GPX4 inactivation, and lipid peroxidation, are manifested in the injured gastrointestinal tract in IBD patients. Furthermore, manipulation of the critical ferroptotic genes could alter the progression, severity, or even morbidity of the experimental colitis. Herein, we critically summarize the recent advances in the field of ferroptosis, focusing on interpreting the potential engagement of ferroptosis in the pathogenesis of IBD. Moreover, we are attempting to shed light on a perspective insight into the possibility of targeting ferroptosis as novel therapeutic designs for the clinical intervention of these gastrointestinal diseases.
MeSH term(s)	Animals ; Chelating Agents/therapeutic use ; Ferroptosis ; Gastrointestinal Diseases/drug therapy ; Gastrointestinal Diseases/metabolism ; Gastrointestinal Diseases/pathology ; Humans
Chemical Substances	Chelating Agents
Language	English
Publishing date	2021-10-25
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2455981-7
ISSN	1942-0994 ; 1942-0994
ISSN (online)	1942-0994
ISSN	1942-0994
DOI	10.1155/2021/4246255
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Article: Partial substitution of fish meal by Clostridium autoethanogenum protein in the diets of juvenile largemouth bass (Micropterus salmoides)

Zhu, Shujie / Gao, Weihua / Wen, Zhengyong / Chi, Shuyan / Shi, Yuhui / Hu, Wei / Tan, Beiping

Aquaculture reports. 2022 Feb., v. 22

2022

Abstract: The present study was conducted to investigate the effect of fish meal replacement with Clostridium autoethanogenum protein (CAP) on growth performance, haemato-biochemistry, digestive capacity, antioxidant index, and histopathological examination of ... ...

Abstract	The present study was conducted to investigate the effect of fish meal replacement with Clostridium autoethanogenum protein (CAP) on growth performance, haemato-biochemistry, digestive capacity, antioxidant index, and histopathological examination of liver and intestine in largemouth bass (Micropterus salmoides). Seven experimental diets were formulated with CAP replacement of 0% (CAP0), 15% (CAP15), 27% (CAP27), 39% (CAP39), 51% (CAP51), 63% (CAP63) and 75% (CAP75) fish meal, respectively. Results showed that replacement of fish meal by CAP did not affect SR, FBW, WGR, SGR and CF. While the fish fed diets containing CAP displayed a higher PER and a lower FCR than the control group (P < 0.05). However, the CAP inclusion level did not statistically affect the whole-body composition (P > 0.05). Additionally, The ADC of dry matter and protein were improved by addition of CAP (P < 0.05), while corresponding ADC of lipid were not significantly changed among different groups (P > 0.05). The lipase and amylase activities were not dramatically altered in stomach (P > 0.05), but were significantly increased in intestine with dietary substitution level up to 39% (P < 0.05). Differently, the protease activity of fish fed CAP75 was higher than other groups in stomach and intestine (P < 0.05). In addition, the activities of T-AOC and SOD in serum, liver and intestine were elevated with dietary CAP level (P < 0.05), whereas MDA content displayed the opposite trend. Moreover, the increased TP, ALB, TC, TG and AST/ALT were observed in treatment groups. In addition, the villus length and width of hindgut were dramatically increased with increasing dietary substitution level up to 39%. Furthermore, no adverse effect on the hepatic histology was occurred when fed diet with substitution level below 63%. Overall, the optimal CAP replacement level was 49.80% with maximum weight gain rate of juvenile largemouth bass.
Keywords	Clostridium autoethanogenum ; Micropterus salmoides ; adverse effects ; amylases ; antioxidants ; aquaculture ; blood serum ; carboxylic ester hydrolases ; enzyme activity ; fish ; fish meal ; growth performance ; hindgut ; histology ; histopathology ; intestines ; juveniles ; lipids ; liver ; stomach ; villi ; weight gain
Language	English
Dates of publication	2022-02
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	2835391-2
ISSN	2352-5134
ISSN	2352-5134
DOI	10.1016/j.aqrep.2021.100938
Database	NAL-Catalogue (AGRICOLA)

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Article: Influences of replacing dietary fish meal by Antarctic krill meal on growth performance, immunity and muscle quality of white shrimp Litopenaeus vannamei

Wei, Yuting / Wang, Xiaoxia / Xie, Fangli / Shen, Haohao / Gao, Weihua / Zhang, Wenbing / Mai, Kangsen

Aquaculture reports. 2022 Aug., v. 25

2022

Abstract: The present study was conducted to investigate the effects of Antarctic krill Euphausia superba meal replacing dietary fish meal on the growth performance, immunity and muscle quality of white shrimp Litopenaeus vannamei (initial body weight: 4.080 ± 0 ... ...

Abstract	The present study was conducted to investigate the effects of Antarctic krill Euphausia superba meal replacing dietary fish meal on the growth performance, immunity and muscle quality of white shrimp Litopenaeus vannamei (initial body weight: 4.080 ± 0.143 g, mean±SD). The replacement levels were designed as 0%, 5%, 15%, 30%, 45%, 60%, 75% and 100%, respectively. After a 52-day feeding trial, results showed that the specific growth rate (P = 0.129) and feed intake (P = 0.217) of shrimp were not significantly affected. Feed conversion ratio (FCR, P = 0.000), protein efficiency ratio (PER, P = 0.000) and protein retention (PR, P = 0.000) were significantly affected with a quadratic positive trend for FCR, a linear and quadratic negative trend for PER and a quadratic negative trend for PR. The total haemocyte count (P = 0.307), respiratory burst (P = 0.168), activities of superoxide dismutase (P = 0.221) and glutathione reductase (P = 0.170) in serum were not significantly affected. While, malondialdehyde content (P = 0.000), acid phosphatase (P = 0.000) and alkaline phosphatase activity (P = 0.000) in serum were significantly decreased both in linear and quadratic trend. The carotenoid content in exoskeleton (P = 0.019) and pH in muscle (P = 0.000) were linearly increased. With the increasing replacement levels, n-3 PUFA content (P = 0.001) in muscle significantly decreased and n-6 PUFA (P = 0.000) increased. The ratio of essential amino acid to total amino acid (P = 0.000) and the inosinic acid content in muscle (P = 0.002) were significantly increased with the dietary replacement levels in a linear trend. In conclusion, Antarctic krill meal can totally replace dietary fish meal without negative impact on the growth of L. vannamei. From the perspective of feed utilization, after the quadratic regression analysis of FCR, PER and PR, the appropriate replacement level of dietary fish meal by krill meal for L. vannamei was 52.78%, 68.50% and 47.41%, respectively (i.e., dietary krill meal level was 13.27%, 17.22%, and 11.92%, respectively).
Keywords	Euphausia superba ; Litopenaeus vannamei ; acid phosphatase ; alkaline phosphatase ; aquaculture ; blood serum ; body weight ; carotenoids ; essential amino acids ; exoskeleton ; feed conversion ; feed intake ; fish meal ; glutathione-disulfide reductase ; growth performance ; hemocytes ; immunity ; krill meal ; malondialdehyde ; muscles ; omega-3 fatty acids ; omega-6 fatty acids ; pH ; protein efficiency ratio ; regression analysis ; respiratory burst ; shrimp ; specific growth rate ; superoxide dismutase
Language	English
Dates of publication	2022-08
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	2835391-2
ISSN	2352-5134
ISSN	2352-5134
DOI	10.1016/j.aqrep.2022.101256
Database	NAL-Catalogue (AGRICOLA)

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Article: Effect of the Interaction between Viral PB2 and Host SphK1 on H9N2 AIV Replication in Mammals

Zhou, Yong / Gao, Weihua / Sun, Yan / Guo, Yuxin / Wu, Yuping / Pu, Juan

Viruses. 2022 July 21, v. 14, no. 7

2022

Abstract: The H9N2 avian influenza virus (AIV) is currently widespread worldwide, posing a severe threat to the poultry industry and public health. Reassortment is an important way for influenza viruses to adapt to a new host. In 2007, the PB2 gene of H9N2 AIV in ... ...

Abstract	The H9N2 avian influenza virus (AIV) is currently widespread worldwide, posing a severe threat to the poultry industry and public health. Reassortment is an important way for influenza viruses to adapt to a new host. In 2007, the PB2 gene of H9N2 AIV in China was reassorted, and the DK1-like lineage replaced the F/98-like lineage, forming a dominant genotype of G57. This genotype and its reassortants (such as H7N9, H10N8 and H5N6) showed higher mammalian adaptation, and caused increased human infections. However, the adaptive mechanisms of the DK1-like lineage PB2 gene remain unclear. Here, we confirmed that the PB2 lineage of the H9N2 AIV currently prevalent in China still belongs to the DK1-like lineage and, compared with the previously predominant F/98-like lineage, the DK1-like lineage PB2 gene significantly enhances H9N2 AIV to mammalian adaptation. Through transcriptomic analysis and qRT–PCR and western blot experiments, we identified a host factor, sphingosine kinase 1 (SphK1), that is closely related to viral replication. SphK1 inhibits the replication of DK1-like PB2 gene H9N2 AIV, but the ability of SphK1 protein to bind DK1-like PB2 protein is weaker than that of F/98-like PB2 protein, which may contribute to H9N2 AIV containing the DK1-like PB2 gene to escape the inhibitory effect of host factor SphK1 for efficient infection. This study broadens our understanding of the adaptive evolution of H9N2 AIV and highlights the necessity to pay close attention to the AIV that contains the adaptive PB2 protein in animals and humans.
Keywords	Influenza A virus ; Western blotting ; evolutionary adaptation ; genes ; genotype ; humans ; influenza ; poultry industry ; public health ; sphingosine ; transcriptomics ; virus replication ; China
Language	English
Dates of publication	2022-0721
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2516098-9
ISSN	1999-4915
ISSN	1999-4915
DOI	10.3390/v14071585
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Article: Dietary phosphorus requirement of red swamp crayfish (Procambarus clarkia)

Xu, Lei / Chen, Xiaoru / Wen, Hua / Wu, Fan / Zhang, Wenbing / Gao, Weihua / Tian, Juan

Aquaculture research. 2022 Mar., v. 53, no. 4

2022

Abstract: To evaluate dietary phosphorus requirement of red swamp crayfish (Procambarus clarkia), six diets containing different concentrations phosphorus (2.7, 6.6, 10.5, 14.6, 18.3 and 22.5 g kg⁻¹ diet respectively) were prepared. Each diet was assigned to ... ...

Abstract	To evaluate dietary phosphorus requirement of red swamp crayfish (Procambarus clarkia), six diets containing different concentrations phosphorus (2.7, 6.6, 10.5, 14.6, 18.3 and 22.5 g kg⁻¹ diet respectively) were prepared. Each diet was assigned to triplicate of 15 crayfish (initial mean weight: 9.33 ± 0.54 g) for 56 days. The weight gain rate (WGR) significantly increased when dietary phosphorus content increased from 2.7 to 14.6 g kg⁻¹ (p < 0.05), and the feed conversion ratio (FCR) showed an opposite tendency as observed in WGR. As dietary phosphorus level increased, the muscular ash content, the content of calcium and phosphorus in muscle and shell, the inorganic phosphorus concentration in serum, and the protease activities in intestine and hepatopancreas significantly increased (p < 0.05), while the muscular crude lipid content, the lipase activities in intestine and hepatopancreas, the malondialdehyde concentration in hepatopancreas, and the concentrations of total cholesterol, triglyceride and parathyroid hormone in serum significantly decreased (p < 0.05). When crayfish fed diet with phosphorus content from 10.5 to 14.6 g kg⁻¹, the activities of amylase in intestine and hepatopancreas, the activities of catalase in hepatopancreas and the concentration of total antioxidant capacity in hepatopancreas significantly increased (p < 0.05). Broken‐line model analysis in terms of the WGR and FCR indicated that optimal dietary phosphorus requirement of crayfish was from 13.9 to 14.3 g kg⁻¹. The signs of phosphorus deficiency for red swamp crayfish were characterized by poor growth, slightly reduced mineralization and increased body lipid deposition.
Keywords	Procambarus clarkii ; amylases ; antioxidant activity ; aquaculture ; ash content ; blood serum ; body fat ; calcium ; carboxylic ester hydrolases ; catalase ; cholesterol ; crayfish ; diet ; feed conversion ; hepatopancreas ; inorganic phosphorus ; intestines ; lipid content ; malondialdehyde ; mineralization ; muscles ; parathyroid hormone ; phosphorus content ; proteinases ; research ; triacylglycerols ; weight gain
Language	English
Dates of publication	2022-03
Size	p. 1293-1303.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	1227359-4
ISSN	1355-557X
ISSN	1355-557X
DOI	10.1111/are.15663
Database	NAL-Catalogue (AGRICOLA)

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Article: Effects of recombinant anti-lipopolysaccharide factor expressed by Pichia pastoris on the growth performance, immune response and disease resistance of Litopenaeusvannamei

Wu, Jing / Lei, Keke / Wu, Zhenhua / Zhang, Yanjiao / Gao, Weihua / Zhang, Wenbing / Mai, Kangsen

Fish & shellfish immunology. 2022 Oct., v. 129

2022

Abstract: The codon-optimized anti-lipopolysaccharide factor (ALF) sequence was introduced into pPICZαA vector and transformed into Pichia pastoris GS115. The recombinant ALF yeast supernatant (rALF-mix) was freeze-dried and evaluated as a feed additive for ... ...

Abstract	The codon-optimized anti-lipopolysaccharide factor (ALF) sequence was introduced into pPICZαA vector and transformed into Pichia pastoris GS115. The recombinant ALF yeast supernatant (rALF-mix) was freeze-dried and evaluated as a feed additive for Litopenaeus vannamei. It was found by antibacterial activity test in vitro that the rALF-mix had antibacterial activity under different pH and temperature conditions. The 0, 0.00375%, 0.0075%, 0.015%, 0.03% and 0.06% of rALF-mix were added respectively to make the six experimental diets. After a 10-week feeding trial with shrimps (2.36 ± 0.02 g), it was found that the weight gain rate (WGR) and protein efficiency ratio (PER) of shrimp in the groups with 0.0075%, 0.015% and 0.03% of dietary rALF-mix supplementation were significantly higher than those in the control group (P < 0.05). Dietary rALF-mix supplementation significantly increased the total haemocyte count, respiratory burst, phagocytic activity, total anti-oxidative capacity (T-AOC), phenol oxidase activity, nitric oxide synthase activity, lysozyme (LYZ) activity, serum antibacterial capacity in the hemolymph and the T-AOC, LYZ in the hepatopancreas of shrimps (P < 0.05). The malondialdehyde contents in hemolymph and hepatopancreas were significantly decreased (P < 0.05). Meanwhile, the expression levels of toll, immune deficiency, heat shock protein 70, crustin and lipopolysaccharide-β-glucan binding protein in the gill of shrimps were significantly increased (P < 0.05). After the challenge test, it was showed that dietary rALF-mix supplementation significantly improved the resistance of L. vannamei to Vibrio parahaemolyticus (P < 0.05). In conclusion, the rALF-mix can be used as a functional feed additive to improve the growth, immunity and disease resistance of shrimp. Based on the quadratic regression analysis for WGR, the optimal supplemental level of rALF-mix in diet for shrimp was estimated to be 0.02813%.
Keywords	Komagataella pastoris ; Litopenaeus vannamei ; Vibrio parahaemolyticus ; antibacterial properties ; antioxidant activity ; blood serum ; crustin ; diet ; disease resistance ; feed additives ; fish ; freeze drying ; growth performance ; heat-shock protein 70 ; hemocytes ; hepatopancreas ; immune response ; lysozyme ; malondialdehyde ; monophenol monooxygenase ; nitric oxide synthase ; pH ; phagocytosis ; protein efficiency ratio ; regression analysis ; respiratory burst ; shellfish ; shrimp ; temperature ; weight gain ; yeasts
Language	English
Dates of publication	2022-10
Size	p. 231-242.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	1067738-0
ISSN	1095-9947 ; 1050-4648
ISSN (online)	1095-9947
ISSN	1050-4648
DOI	10.1016/j.fsi.2022.08.074
Database	NAL-Catalogue (AGRICOLA)

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