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  1. Article ; Online: Aging in Indigenous Communities: Perspective from Two Ancestral Communities in the Colombian Andean-Amazon Region.

    García, Hernán D / García, Wilson A / Curcio, Carmen L

    Journal of cross-cultural gerontology

    2024  Volume 39, Issue 2, Page(s) 189–205

    Abstract: The phenomenon of world aging is not foreign to indigenous communities. In the last few years, research about these communities around the world has increased, but aging in indigenous towns still has not been studied widely. The purpose of this research ... ...

    Abstract The phenomenon of world aging is not foreign to indigenous communities. In the last few years, research about these communities around the world has increased, but aging in indigenous towns still has not been studied widely. The purpose of this research is to interpret the meaning of old age in two indigenous communities from the Colombian Andean-Amazon region (the Inga and Kamëntsa) to reinforce the relevance of the local sociocultural context within the configuration of the meaning of old age and to emphasize the importance of considering particular regional characteristics for the design of policies and interventions aiming to recognize and integrate indigenous populations. This is a qualitative study with an interactionism-symbolism approach. In total, six indigenous people older than 60 years from two ancestral communities from the Colombian Andean-Amazon region participated in the in-depth interviews. Data analysis was carried out in three moments: discovery, coding, and relativization of the information. The results show that old age means wisdom, "I am wise," which is supported in the cosmology and the trajectory of life, reinforces the identity and autonomy, and allows them to be agents in the dynamics of their communities from the "I do," in other words, their roles as builders of the family-society and as guards of ancestral knowledge. The loss of this knowledge and the elements that it is composed of uproot them and put them at risk of disappearing as individuals and as a collective. In conclusion, the meaning of old age in these communities is not centered on a determinate age; you are not old, you are wise, and as such, they play a central role in their communities. Moreover, wisdom is built in parallel with their cosmology and assigns them the task of safekeeping ancestral knowledge. In order to do this, they use oral tradition as a tool, words that are born in their territories, travel in a nonlinear timeline, and get strengthened by the community while also protecting it and building it. Knowing what aging means for Indigenous communities can facilitate to the development of policies and initiatives and to provide culturally appropriate and effective programs.
    MeSH term(s) Humans ; Colombia/ethnology ; Female ; Male ; Aging/ethnology ; Qualitative Research ; Aged ; Indians, South American ; Middle Aged ; Indigenous Peoples ; Interviews as Topic ; Aged, 80 and over
    Language English
    Publishing date 2024-05-08
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 644348-5
    ISSN 1573-0719 ; 0169-3816
    ISSN (online) 1573-0719
    ISSN 0169-3816
    DOI 10.1007/s10823-023-09495-1
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  2. Article ; Online: Structural and Theoretical Study of Copper(II)-5-fluoro Uracil Acetate Coordination Compounds: Single-Crystal to Single-Crystal Transformation as Possible Humidity Sensor.

    Vegas, Verónica G / García-Hernán, Andrea / Aguilar-Galindo, Fernando / Perles, Josefina / Amo-Ochoa, Pilar

    Polymers

    2023  Volume 15, Issue 13

    Abstract: This paper describes the synthesis and characterization of seven different copper(II) coordination compounds, as well as the formation of a protonated ligand involving all compounds from the same reaction. Their synthesis required hydrothermal conditions, ...

    Abstract This paper describes the synthesis and characterization of seven different copper(II) coordination compounds, as well as the formation of a protonated ligand involving all compounds from the same reaction. Their synthesis required hydrothermal conditions, causing the partial in situ transformation of 5-fluoro uracil-1-acetic acid (5-FUA) into an oxalate ion (ox), as well as the protonation of the 4,4'-bipyridine (bipy) ligand through a catalytic process resulting from the presence of Cu(II) within the reaction. These initial conditions allowed obtaining the new coordination compounds
    Language English
    Publishing date 2023-06-26
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527146-5
    ISSN 2073-4360 ; 2073-4360
    ISSN (online) 2073-4360
    ISSN 2073-4360
    DOI 10.3390/polym15132827
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  3. Article ; Online: Competing constraints shape the nonequilibrium limits of cellular decision-making.

    Lammers, Nicholas C / Flamholz, Avi I / Garcia, Hernan G

    Proceedings of the National Academy of Sciences of the United States of America

    2023  Volume 120, Issue 10, Page(s) e2211203120

    Abstract: Gene regulation is central to cellular function. Yet, despite decades of work, we lack quantitative models that can predict how transcriptional control emerges from molecular interactions at the gene locus. Thermodynamic models of transcription, which ... ...

    Abstract Gene regulation is central to cellular function. Yet, despite decades of work, we lack quantitative models that can predict how transcriptional control emerges from molecular interactions at the gene locus. Thermodynamic models of transcription, which assume that gene circuits operate at equilibrium, have previously been employed with considerable success in the context of bacterial systems. However, the presence of ATP-dependent processes within the eukaryotic transcriptional cycle suggests that equilibrium models may be insufficient to capture how eukaryotic gene circuits sense and respond to input transcription factor concentrations. Here, we employ simple kinetic models of transcription to investigate how energy dissipation within the transcriptional cycle impacts the rate at which genes transmit information and drive cellular decisions. We find that biologically plausible levels of energy input can lead to significant gains in how rapidly gene loci transmit information but discover that the regulatory mechanisms underlying these gains change depending on the level of interference from noncognate activator binding. When interference is low, information is maximized by harnessing energy to push the sensitivity of the transcriptional response to input transcription factors beyond its equilibrium limits. Conversely, when interference is high, conditions favor genes that harness energy to increase transcriptional specificity by proofreading activator identity. Our analysis further reveals that equilibrium gene regulatory mechanisms break down as transcriptional interference increases, suggesting that energy dissipation may be indispensable in systems where noncognate factor interference is sufficiently large.
    MeSH term(s) Eukaryotic Cells ; Eukaryota ; Gene Regulatory Networks ; Kinetics ; Thermodynamics ; Transcription Factors/genetics
    Chemical Substances Transcription Factors
    Language English
    Publishing date 2023-03-02
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.2211203120
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  4. Article: Unified bursting strategies in ectopic and endogenous

    Berrocal, Augusto / Lammers, Nicholas C / Garcia, Hernan G / Eisen, Michael B

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Transcription often occurs in bursts as gene promoters switch stochastically between active and inactive states. Enhancers can dictate transcriptional activity in animal development through the modulation of burst frequency, duration, or amplitude. ... ...

    Abstract Transcription often occurs in bursts as gene promoters switch stochastically between active and inactive states. Enhancers can dictate transcriptional activity in animal development through the modulation of burst frequency, duration, or amplitude. Previous studies observed that different enhancers can achieve a wide range of transcriptional outputs through the same strategies of bursting control. For example, despite responding to different transcription factors, all
    Language English
    Publishing date 2023-02-10
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.02.09.527927
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  5. Article ; Online: Incidence of type 1 diabetes in Chilean children between 2006 and 2021: significant increase during the COVID-19 pandemic.

    Tampe, Ingeborg / Garfias, Carolina / Borzutzky, Arturo / Slaibe, Lissette / García, Hernan

    Acta diabetologica

    2023  Volume 61, Issue 1, Page(s) 29–34

    Abstract: Aims: An increase in type 1 diabetes (T1D) incidence has been observed in several countries during the COVID-19 pandemic. The objective of this study is to determine T1D incidence trends in Chilean children between 2006 and 2021, and specifically ... ...

    Abstract Aims: An increase in type 1 diabetes (T1D) incidence has been observed in several countries during the COVID-19 pandemic. The objective of this study is to determine T1D incidence trends in Chilean children between 2006 and 2021, and specifically evaluate the effect of the COVID-19 pandemic in this population.
    Methods: We reviewed mandatory notifications of T1D in Chile's public and private health system in youth < 20 years between 2006 and 2021, and compared COVID-19 pre-pandemic and pandemic incidence.
    Results: In Chile, 9472 new T1D cases in children were confirmed between 2006 and 2021. The mean annual T1D incidence in the entire period was 12.7/100,000 inhabitants, with an incidence of 11.7/100,000 between 2006 and 2019 vs. 20.2/100,000 during 2020-2021 (β = 0.691, [95%CI 0.479-0903], p < 0.001.) The highest incidence was observed in the 10-14 years age group, but a significant increasing incidence was observed in all age groups. The second year of the COVID-19 pandemic, 2021, had the highest incidence rate of the study period. While a 5% mean annual increase was observed between 2006 and 2019, in 2021 the T1D incidence jumped 28.5% compared with the two previous years. We found a higher T1D incidence in population with private insurance than public insurance (14.8 vs. 11.7/100.000, respectively, RR = 1.26 [95%CI 1.03-1.53], p < 0.027).
    Conclusions: T1D incidence rates in Chilean youth doubled between 2006 and 2018, subsequently presenting a striking increase during the COVID-19 pandemic.
    MeSH term(s) Child ; Adolescent ; Humans ; Diabetes Mellitus, Type 1/epidemiology ; Incidence ; Pandemics ; Chile/epidemiology ; COVID-19/epidemiology
    Language English
    Publishing date 2023-08-14
    Publishing country Germany
    Document type Review ; Journal Article
    ZDB-ID 1097676-0
    ISSN 1432-5233 ; 0940-5429
    ISSN (online) 1432-5233
    ISSN 0940-5429
    DOI 10.1007/s00592-023-02163-3
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  6. Article ; Online: A bistable autoregulatory module in the developing embryo commits cells to binary expression fates.

    Zhao, Jiaxi / Perkins, Mindy Liu / Norstad, Matthew / Garcia, Hernan G

    Current biology : CB

    2023  Volume 33, Issue 14, Page(s) 2851–2864.e11

    Abstract: Bistable autoactivation has been proposed as a mechanism for cells to adopt binary fates during embryonic development. However, it is unclear whether the autoactivating modules found within developmental gene regulatory networks are bistable, unless ... ...

    Abstract Bistable autoactivation has been proposed as a mechanism for cells to adopt binary fates during embryonic development. However, it is unclear whether the autoactivating modules found within developmental gene regulatory networks are bistable, unless their parameters are quantitatively determined. Here, we combine in vivo live imaging with mathematical modeling to dissect the binary cell fate dynamics of the fruit fly pair-rule gene fushi tarazu (ftz), which is regulated by two known enhancers: the early (non-autoregulating) element and the autoregulatory element. Live imaging of transcription and protein concentration in the blastoderm revealed that binary Ftz fates are achieved as Ftz expression rapidly transitions from being dictated by the early element to the autoregulatory element. Moreover, we discovered that Ftz concentration alone is insufficient to activate the autoregulatory element, and that this element only becomes responsive to Ftz at a prescribed developmental time. Based on these observations, we developed a dynamical systems model and quantitated its kinetic parameters directly from experimental measurements. Our model demonstrated that the ftz autoregulatory module is indeed bistable and that the early element transiently establishes the content of the binary cell fate decision to which the autoregulatory module then commits. Further in silico analysis revealed that the autoregulatory element locks the Ftz fate quickly, within 35 min of exposure to the transient signal of the early element. Overall, our work confirms the widely held hypothesis that autoregulation can establish developmental fates through bistability and, most importantly, provides a framework for the quantitative dissection of cellular decision-making.
    MeSH term(s) Animals ; Homeodomain Proteins/genetics ; Fushi Tarazu Transcription Factors/metabolism ; Drosophila Proteins/metabolism ; Drosophila/genetics ; Homeostasis
    Chemical Substances Homeodomain Proteins ; Fushi Tarazu Transcription Factors ; Drosophila Proteins
    Language English
    Publishing date 2023-07-14
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1071731-6
    ISSN 1879-0445 ; 0960-9822
    ISSN (online) 1879-0445
    ISSN 0960-9822
    DOI 10.1016/j.cub.2023.06.060
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  7. Article ; Online: Structural and Theoretical Study of Copper(II)-5-fluoro Uracil Acetate Coordination Compounds

    Verónica G. Vegas / Andrea García-Hernán / Fernando Aguilar-Galindo / Josefina Perles / Pilar Amo-Ochoa

    Polymers, Vol 15, Iss 2827, p

    Single-Crystal to Single-Crystal Transformation as Possible Humidity Sensor

    2023  Volume 2827

    Abstract: This paper describes the synthesis and characterization of seven different copper(II) coordination compounds, as well as the formation of a protonated ligand involving all compounds from the same reaction. Their synthesis required hydrothermal conditions, ...

    Abstract This paper describes the synthesis and characterization of seven different copper(II) coordination compounds, as well as the formation of a protonated ligand involving all compounds from the same reaction. Their synthesis required hydrothermal conditions, causing the partial in situ transformation of 5-fluoro uracil-1-acetic acid (5-FUA) into an oxalate ion (ox), as well as the protonation of the 4,4′-bipyridine (bipy) ligand through a catalytic process resulting from the presence of Cu(II) within the reaction. These initial conditions allowed obtaining the new coordination compounds [Cu 2 (5-FUA) 2 (ox)(bipy)] n ·2n H 2 O (CP2) , [Cu(5-FUA) 2 (H 2 O)(bipy)] n ·2n H 2 O (CP3) , as well as the ionic pair [(H 2 bipy) +2 2NO 3 − ] (1) . The mother liquor evolved rapidly at room temperature and atmospheric pressure, due to the change in concentration of the initial reagents and the presence of the new chemical species generated in the reaction process, yielding CPs [Cu(5-FUA) 2 (bipy)] n ·3.5n H 2 O , [Cu 3 (ox) 3 (bipy) 4 ] n and [Cu(ox)(bipy)] n . The molecular compound [Cu(5-FUA) 2 (H 2 O) 4 ]·4H 2 O (more thermodynamically stable) ended up in the mother liquor after filtration at longer reaction times at 25 °C and 1 atm., cohabiting in the medium with the other crystalline solids in different proportions. In addition, the evaporation of H 2 O caused the single-crystal to single-crystal transformation (SCSC) of [Cu(5-FUA) 2 (H 2 O)(bipy)] n ·2n H 2 O (CP3) into [Cu(5-FUA) 2 (bipy)] n ·2n H 2 O (CP4) . A theoretical study was performed to analyze the thermodynamic stability of the phases. The observed SCSC transformation also involved a perceptible color change, highlighting this compound as a possible water sensor.
    Keywords copper ; humidity sensors ; coordination compounds ; coordination polymers ; theoretical calculations ; Organic chemistry ; QD241-441
    Subject code 290
    Language English
    Publishing date 2023-06-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Determining Factors to Understand the External Quantum Efficiency Values: Study Carried Out with Copper(I)-I and 1,2-Bis(4-pyridyl)ethane Coordination Polymers as Downshifters in Photovoltaic Modules.

    García-Hernán, Andrea / Brito-Santos, Gabriela / de la Rubia, Elena / Aguilar-Galindo, Fernando / Castillo, Oscar / Lifante-Pedrola, Ginés / Sanchiz, Joaquín / Guerrero-Lemus, Ricardo / Amo-Ochoa, Pilar

    Inorganic chemistry

    2024  Volume 63, Issue 10, Page(s) 4646–4656

    Abstract: Downshifters refer to compounds with the capacity to absorb UV photons and transform them into visible light. The integration of such downshifters has the potential to improve the efficiency of commercial photovoltaic modules. Initially, costly ... ...

    Abstract Downshifters refer to compounds with the capacity to absorb UV photons and transform them into visible light. The integration of such downshifters has the potential to improve the efficiency of commercial photovoltaic modules. Initially, costly lanthanide derivatives and organic fluorescent dyes were introduced, resulting in a heightened module efficiency. In a novel research direction guided by the same physicochemical principles, the utilization of copper(I) coordination compounds is proposed. This choice is motivated by its simpler and more economical synthesis, primarily due to copper being a more abundant and less toxic element. Our proposal involves employing 1,2-bis(4-pyridyl) ethane (
    Language English
    Publishing date 2024-03-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1484438-2
    ISSN 1520-510X ; 0020-1669
    ISSN (online) 1520-510X
    ISSN 0020-1669
    DOI 10.1021/acs.inorgchem.3c04232
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  9. Article ; Online: Reseña de

    Enrique García Hernán

    Espacio, Tiempo y Forma. Serie IV, Historia Moderna, Vol 0, Iss 29, Pp 297-

    Joseph Pérez, Cisneros, el cardenal de España, Taurus, 2014, 368 pp. isbn 978-84-306-0948-2

    2016  Volume 300

    Abstract: Reseña de: Joseph Pérez, Cisneros, el cardenal de España, Taurus, 2014, 368 pp. isbn 978-84-306-0948- ... ...

    Abstract Reseña de: Joseph Pérez, Cisneros, el cardenal de España, Taurus, 2014, 368 pp. isbn 978-84-306-0948-2
    Keywords History (General) and history of Europe ; D ; History (General) ; D1-2009 ; Modern history ; 1453- ; D204-475
    Language English
    Publishing date 2016-12-01T00:00:00Z
    Publisher Universidad Nacional de Educación a Distancia (UNED)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Optogenetic control of YAP reveals a dynamic communication code for stem cell fate and proliferation.

    Meyer, Kirstin / Lammers, Nicholas C / Bugaj, Lukasz J / Garcia, Hernan G / Weiner, Orion D

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 6929

    Abstract: YAP is a transcriptional regulator that controls pluripotency, cell fate, and proliferation. How cells ensure the selective activation of YAP effector genes is unknown. This knowledge is essential to rationally control cellular decision-making. Here we ... ...

    Abstract YAP is a transcriptional regulator that controls pluripotency, cell fate, and proliferation. How cells ensure the selective activation of YAP effector genes is unknown. This knowledge is essential to rationally control cellular decision-making. Here we leverage optogenetics, live-imaging of transcription, and cell fate analysis to understand and control gene activation and cell behavior. We reveal that cells decode the steady-state concentrations and timing of YAP activation to control proliferation, cell fate, and expression of the pluripotency regulators Oct4 and Nanog. While oscillatory YAP inputs induce Oct4 expression and proliferation optimally at frequencies that mimic native dynamics, cellular differentiation requires persistently low YAP levels. We identify the molecular logic of the Oct4 dynamic decoder, which acts through an adaptive change sensor. Our work reveals how YAP levels and dynamics enable multiplexing of information transmission for the regulation of developmental decision-making and establishes a platform for the rational control of these behaviors.
    MeSH term(s) Cell Differentiation/genetics ; Cell Proliferation/genetics ; Optogenetics ; Stem Cells ; Cell Communication
    Language English
    Publishing date 2023-10-30
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-42643-2
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