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  1. Article ; Online: Genetic modification of

    Chávez-Tinoco, Marco / García-Ortega, Luis F / Mancera, Eugenio

    Microbiology (Reading, England)

    2024  Volume 170, Issue 3

    Abstract: ... Candida ... ...

    Abstract Candida maltosa
    MeSH term(s) Humans ; Candida/genetics ; Candida albicans/genetics ; Candida tropicalis/genetics ; Biological Evolution
    Language English
    Publishing date 2024-03-08
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1180712-x
    ISSN 1465-2080 ; 1350-0872
    ISSN (online) 1465-2080
    ISSN 1350-0872
    DOI 10.1099/mic.0.001447
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Conservation of the Pal/Rim Pathway in Ustilaginomycetes.

    Martínez-Soto, Domingo / García-Ortega, Luis F / Guzmán-Rincón, Andrés / Ortiz-Castellanos, Lucila / León-Ramírez, Claudia G

    Current microbiology

    2024  Volume 81, Issue 7, Page(s) 173

    Abstract: The ability of fungi to effectively sense and internalize signals related to extracellular changing environments is essential for survival. This adaptability is particularly important for fungal pathogens of humans and plants that must sense and respond ... ...

    Abstract The ability of fungi to effectively sense and internalize signals related to extracellular changing environments is essential for survival. This adaptability is particularly important for fungal pathogens of humans and plants that must sense and respond to drastic environmental changes when colonizing their hosts. One of the most important physicochemical factors affecting fungal growth and development is the pH. Ascomycota fungal species possess mechanisms such as the Pal/Rim pathway for external pH sensing and adaptation. However, the conservation of this mechanism in other fungi, such as Ustilaginomycetes is still little studied. To overcome this knowledge gap, we used a comparative genomic approach to explore the conservation of the Pal/Rim pathway in the 13 best sequenced and annotated Ustilaginomycetes. Our findings reveal that the Rim proteins and the Endosomal Sorting Complex Required for Transport (ESCRT) proteins are conserved in Ustilaginomycetes. They conserve the canonical domains present in Pal/Rim and ESCRT proteins of Ascomycota. This study sheds light on the molecular mechanisms used by these fungi for responding to extracellular stresses such as the pH, and open the door to further experimentations for understanding the molecular bases of the signaling in Ustilaginomycetes.
    MeSH term(s) Fungal Proteins/genetics ; Fungal Proteins/metabolism ; Hydrogen-Ion Concentration ; Signal Transduction ; Ascomycota/genetics ; Ascomycota/metabolism ; Endosomal Sorting Complexes Required for Transport/metabolism ; Endosomal Sorting Complexes Required for Transport/genetics ; Phylogeny
    Language English
    Publishing date 2024-05-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 134238-1
    ISSN 1432-0991 ; 0343-8651
    ISSN (online) 1432-0991
    ISSN 0343-8651
    DOI 10.1007/s00284-024-03696-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  3. Article: Whole-Genome DNA Methylation Analysis in Hydrogen Peroxide Overproducing Transgenic Tobacco Resistant to Biotic and Abiotic Stresses.

    Villagómez-Aranda, Ana L / García-Ortega, Luis F / Torres-Pacheco, Irineo / Guevara-González, Ramón G

    Plants (Basel, Switzerland)

    2021  Volume 10, Issue 1

    Abstract: Epigenetic regulation is a key component of stress responses, acclimatization and adaptation processes in plants. DNA methylation is a stable mark plausible for the inheritance of epigenetic traits, such that it is a potential scheme for plant breeding. ... ...

    Abstract Epigenetic regulation is a key component of stress responses, acclimatization and adaptation processes in plants. DNA methylation is a stable mark plausible for the inheritance of epigenetic traits, such that it is a potential scheme for plant breeding. However, the effect of modulators of stress responses, as hydrogen peroxide (H
    Language English
    Publishing date 2021-01-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704341-1
    ISSN 2223-7747
    ISSN 2223-7747
    DOI 10.3390/plants10010178
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  4. Article: Whole-Genome DNA Methylation Analysis in Hydrogen Peroxide Overproducing Transgenic Tobacco Resistant to Biotic and Abiotic Stresses

    Villagómez-Aranda, Ana L / García-Ortega, Luis F / Torres-Pacheco, Irineo / Guevara-González, Ramón G

    Plants. 2021 Jan. 19, v. 10, no. 1

    2021  

    Abstract: Epigenetic regulation is a key component of stress responses, acclimatization and adaptation processes in plants. DNA methylation is a stable mark plausible for the inheritance of epigenetic traits, such that it is a potential scheme for plant breeding. ... ...

    Abstract Epigenetic regulation is a key component of stress responses, acclimatization and adaptation processes in plants. DNA methylation is a stable mark plausible for the inheritance of epigenetic traits, such that it is a potential scheme for plant breeding. However, the effect of modulators of stress responses, as hydrogen peroxide (H₂O₂), in the methylome status has not been elucidated. A transgenic tobacco model to the CchGLP gene displayed high H₂O₂ endogen levels correlated with biotic and abiotic stresses resistance. The present study aimed to determine the DNA methylation status changes in the transgenic model to obtain more information about the molecular mechanism involved in resistance phenotypes. The Whole-genome bisulfite sequencing analysis revealed a minimal impact of overall levels and distribution of methylation. A total of 9432 differential methylated sites were identified in distinct genome regions, most of them in CHG context, with a trend to hypomethylation. Of these, 1117 sites corresponded to genes, from which 83 were also differentially expressed in the plants. Several genes were associated with respiration, energy, and calcium signaling. The data obtained highlighted the relevance of the H₂O₂ in the homeostasis of the system in stress conditions, affecting at methylation level and suggesting an association of the H₂O₂ in the physiological adaptation to stress functional linkages may be regulated in part by DNA methylation.
    Keywords DNA methylation ; abiotic stress ; acclimation ; bisulfites ; calcium signaling ; energy ; epigenetics ; gene expression regulation ; genes ; genetically modified organisms ; homeostasis ; hydrogen peroxide ; information ; models ; phenotype ; plant breeding ; sequence analysis ; stress response ; tobacco
    Language English
    Dates of publication 2021-0119
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    Note NAL-light
    ZDB-ID 2704341-1
    ISSN 2223-7747
    ISSN 2223-7747
    DOI 10.3390/plants10010178
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  5. Article ; Online: Draft Genome Sequence of a Kazachstania humilis Strain Isolated from Agave Fermentation.

    García-Ortega, Luis F / Colón-González, Maritrini / Sedeño, Iván / Santiago-Garduño, Erick / Avelar-Rivas, J Abraham / Kirchmayr, Manuel R / DeLuna, Alexander / Delaye, Luis / Morales, Lucia / Mancera, Eugenio

    Microbiology resource announcements

    2022  Volume 11, Issue 3, Page(s) e0115421

    Abstract: The ascomycetous yeast Kazachstania humilis is an active species in backslopped sourdough and in the spontaneous fermentation of several traditional foods and beverages. Here, we report the draft genome sequence of ... ...

    Abstract The ascomycetous yeast Kazachstania humilis is an active species in backslopped sourdough and in the spontaneous fermentation of several traditional foods and beverages. Here, we report the draft genome sequence of a
    Language English
    Publishing date 2022-03-02
    Publishing country United States
    Document type Journal Article
    ISSN 2576-098X
    ISSN (online) 2576-098X
    DOI 10.1128/mra.01154-21
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Response of transgenic tobacco overexpressing the CchGLP gene to cadmium and aluminium: phenotypic and microRNAs expression changes

    Sáenz-de la O, Diana / Cedillo-Jimenez, Christopher Alexis / García-Ortega, Luis F / Martínez-Reséndiz, Mariela / Arné-Robles, Diego / Cruz-Hernandez, Andrés / Guevara-Gonzalez, Ramón Gerardo

    Physiology and molecular biology of plants. 2020 Jan., v. 26, no. 1

    2020  

    Abstract: Transgenic tobacco (N. tabacum cv. Xanthi nc) expressing Capsicum chinense CchGLP gene that encodes an Mn-SOD, constitutively produces hydrogen peroxide that increase endogenous ROS levels. Previous studies using these plants against geminivirus ... ...

    Abstract Transgenic tobacco (N. tabacum cv. Xanthi nc) expressing Capsicum chinense CchGLP gene that encodes an Mn-SOD, constitutively produces hydrogen peroxide that increase endogenous ROS levels. Previous studies using these plants against geminivirus infections as well as drought stress confirmed that CchGLP expression conferred resistance against biotic and abiotic stresses. Cadmium (Cd) and Aluminium (Al) contamination in soils are a major ecological concern since they are two of the most widespread toxic elements in terrestrial environments. Trying to explore additional possible tolerance to another stresses in these plants, the aim of this work was to analyse the response to cadmium and aluminium salts during germination and early stages of plantlet development and a differential transcriptome of microRNAs (miRNAs) expression in expressing CchGLP transgenic lines and an azygote non-CchGLP expressing line. Plants were grown in vitro with addition of CdCl2 and AlCl3 at three different concentrations: 100, 300 and 500 μM and 50, 150 and 300 μM, respectively. The results showed higher tolerance to Cd and Al salts evaluated in two CchGLP-expressing transgenic lines L8 and L26 in comparison with the azygous non-CchGLP expressing line L1. Interestingly, L8 under Al stress presented vigorous roots and development of radicular hairs in comparison with azygous control (L1). Differentially expressed miRNAs in the comparison between L8 and L1 were associated with up and down-regulation of target genes related with structural molecule activity and ribosome constituents, as well as down-regulation in proton-transporting V-type ATPase (Vacuolar ATPase or V-ATPase). Moreover, KEGG analysis of the target genes for the differentially expressed miRNAs, led to identification of genes related with metabolic pathways and biosynthesis of secondary metabolites. One possible explanation of the tolerance to Cd and Al displayed in the transgenic tobaccos evaluated, might involve the fact that several down-regulated miRNAs, were found associated with target genes expressing V-ATPase. Specifically, miR7904-5p was down regulated and related with the up-regulation of one V-ATPase. The expression levels of these genes was confirmed by qRT-PCR assays, thus suggesting that a cation transport activity driven by the V-ATPases-dependent proton motive force, might significantly contribute as one mechanism for Cd and Al detoxification by vacuolar compartmentation in these transgenic tobacco plants.
    Keywords Capsicum chinense ; Geminiviridae ; H-transporting ATP synthase ; aluminum ; aluminum chloride ; biochemical pathways ; biosynthesis ; biotic stress ; cadmium ; cadmium chloride ; cations ; gene expression regulation ; gene overexpression ; genes ; germination ; hydrogen peroxide ; microRNA ; phenotype ; plantlets ; proton-motive force ; quantitative polymerase chain reaction ; reverse transcriptase polymerase chain reaction ; ribosomes ; root hairs ; secondary metabolites ; soil ; superoxide dismutase ; tobacco ; toxic substances ; transcriptome ; transgenic plants ; vacuoles ; water stress
    Language English
    Dates of publication 2020-01
    Size p. 3-13.
    Publishing place Springer India
    Document type Article
    ZDB-ID 2487126-6
    ISSN 0974-0430 ; 0971-5894
    ISSN (online) 0974-0430
    ISSN 0971-5894
    DOI 10.1007/s12298-019-00716-x
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  7. Article ; Online: Yeast diversity in open agave fermentations across Mexico.

    Gallegos-Casillas, Porfirio / García-Ortega, Luis F / Espinosa-Cantú, Adriana / Avelar-Rivas, J Abraham / Torres-Lagunes, Carolina G / Cano-Ricardez, Adrián / García-Acero, Ángela M / Ruiz-Castro, Susana / Flores-Barraza, Mayra / Castillo, Alejandra / González-Zozaya, Fernando / Delgado-Lemus, América / Molina-Freaner, Francisco / Jacques-Hernández, Cuauhtémoc / Hernández-López, Antonio / Delaye, Luis / Aguirre-Dugua, Xitlali / Kirchmayr, Manuel R / Morales, Lucia /
    Mancera, Eugenio / DeLuna, Alexander

    Yeast (Chichester, England)

    2023  Volume 41, Issue 1-2, Page(s) 35–51

    Abstract: Yeasts are a diverse group of fungal microorganisms that are widely used to produce fermented foods and beverages. In Mexico, open fermentations are used to obtain spirits from agave plants. Despite the prevalence of this traditional practice throughout ... ...

    Abstract Yeasts are a diverse group of fungal microorganisms that are widely used to produce fermented foods and beverages. In Mexico, open fermentations are used to obtain spirits from agave plants. Despite the prevalence of this traditional practice throughout the country, yeasts have only been isolated and studied from a limited number of distilleries. To systematically describe the diversity of yeast species from open agave fermentations, here we generate the YMX-1.0 culture collection by isolating 4524 strains from 68 sites with diverse climatic, geographical, and biological contexts. We used MALDI-TOF mass spectrometry for taxonomic classification and validated a subset of the strains by ITS and D1/D2 sequencing, which also revealed two potential novel species of Saccharomycetales. Overall, the composition of yeast communities was weakly associated with local variables and types of climate, yet a core set of six species was consistently isolated from most producing regions. To explore the intraspecific variation of the yeasts from agave fermentations, we sequenced the genomes of four isolates of the nonconventional yeast Kazachstania humilis. The genomes of these four strains were substantially distinct from a European isolate of the same species, suggesting that they may belong to different populations. Our work contributes to the understanding and conservation of an open fermentation system of great cultural and economic importance, providing a valuable resource to study the biology and genetic diversity of microorganisms living at the interface of natural and human-associated environments.
    MeSH term(s) Humans ; Fermentation ; Agave/microbiology ; Mexico ; Yeasts ; Alcoholic Beverages/microbiology
    Language English
    Publishing date 2023-12-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 632636-5
    ISSN 1097-0061 ; 0749-503X
    ISSN (online) 1097-0061
    ISSN 0749-503X
    DOI 10.1002/yea.3913
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  8. Article: Response of transgenic tobacco overexpressing the

    Sáenz-de la O, Diana / Cedillo-Jimenez, Christopher Alexis / García-Ortega, Luis F / Martínez-Reséndiz, Mariela / Arné-Robles, Diego / Cruz-Hernandez, Andrés / Guevara-Gonzalez, Ramón Gerardo

    Physiology and molecular biology of plants : an international journal of functional plant biology

    2019  Volume 26, Issue 1, Page(s) 3–13

    Abstract: Transgenic tobacco ( ...

    Abstract Transgenic tobacco (
    Language English
    Publishing date 2019-10-23
    Publishing country India
    Document type Journal Article
    ZDB-ID 2487126-6
    ISSN 0974-0430 ; 0971-5894
    ISSN (online) 0974-0430
    ISSN 0971-5894
    DOI 10.1007/s12298-019-00716-x
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  9. Article ; Online: Transcriptional landscapes of Axolotl (Ambystoma mexicanum).

    Caballero-Pérez, Juan / Espinal-Centeno, Annie / Falcon, Francisco / García-Ortega, Luis F / Curiel-Quesada, Everardo / Cruz-Hernández, Andrés / Bako, Laszlo / Chen, Xuemei / Martínez, Octavio / Alberto Arteaga-Vázquez, Mario / Herrera-Estrella, Luis / Cruz-Ramírez, Alfredo

    Developmental biology

    2018  Volume 433, Issue 2, Page(s) 227–239

    Abstract: The axolotl (Ambystoma mexicanum) is the vertebrate model system with the highest regeneration capacity. Experimental tools established over the past 100 years have been fundamental to start unraveling the cellular and molecular basis of tissue and limb ... ...

    Abstract The axolotl (Ambystoma mexicanum) is the vertebrate model system with the highest regeneration capacity. Experimental tools established over the past 100 years have been fundamental to start unraveling the cellular and molecular basis of tissue and limb regeneration. In the absence of a reference genome for the Axolotl, transcriptomic analysis become fundamental to understand the genetic basis of regeneration. Here we present one of the most diverse transcriptomic data sets for Axolotl by profiling coding and non-coding RNAs from diverse tissues. We reconstructed a population of 115,906 putative protein coding mRNAs as full ORFs (including isoforms). We also identified 352 conserved miRNAs and 297 novel putative mature miRNAs. Systematic enrichment analysis of gene expression allowed us to identify tissue-specific protein-coding transcripts. We also found putative novel and conserved microRNAs which potentially target mRNAs which are reported as important disease candidates in heart and liver.
    MeSH term(s) Ambystoma mexicanum/genetics ; Ambystoma mexicanum/physiology ; Animals ; Female ; Gene Expression Regulation ; Gene Library ; Gene Ontology ; Humans ; MicroRNAs/biosynthesis ; MicroRNAs/genetics ; Organ Specificity ; Principal Component Analysis ; RNA, Messenger/biosynthesis ; RNA, Messenger/genetics ; RNA, Small Interfering/genetics ; Regeneration/genetics ; Sequence Analysis, RNA ; Species Specificity ; Transcription, Genetic ; Transcriptome
    Chemical Substances MicroRNAs ; RNA, Messenger ; RNA, Small Interfering
    Language English
    Publishing date 2018--15
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1114-9
    ISSN 1095-564X ; 0012-1606
    ISSN (online) 1095-564X
    ISSN 0012-1606
    DOI 10.1016/j.ydbio.2017.08.022
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