Article ; Online: Evaluation of Clastogenic/Aneugenic Damage Using the FISH Micronucleus Assay in Mice Exposed to Chromium (VI).
2023 Volume 37, Issue 4, Page(s) 1666–1671
Abstract: Background/aim: Exposure to chromium (VI) [Cr(VI)] has been postulated to be associated with the induction of cancer. In vivo studies utilizing biomarkers of genotoxic damage could aid in elucidating the mechanisms underlying the genotoxic effects of Cr( ...
Abstract | Background/aim: Exposure to chromium (VI) [Cr(VI)] has been postulated to be associated with the induction of cancer. In vivo studies utilizing biomarkers of genotoxic damage could aid in elucidating the mechanisms underlying the genotoxic effects of Cr(VI) and their relationship with carcinogenesis. In this study, the origin (clastogenic and/or aneugenic damage) and kinetics of micronuclei (MN) induced by Cr(VI) were investigated. Materials and methods: Hsd:ICR female mice were divided into groups of five individuals each. MN kinetics were measured in groups treated with 20 or 25 mg/kg CrO Results: Total MN were quantified and the clastogenic/aneugenic effects of Cr(VI) were evaluated based on the proportion of MNK+ versus micronuclei without centromeric DNA (MNK-). There was a significant increase in MN frequencies beginning at 12 h in the Cr(VI)-treated groups demonstrating its genotoxicity. When calculating the MNK+ as a percentage of the total MN, the increase was significant beginning 12 h after treatment. Conclusion: The fact that the MNK+ and MNK- were observed at both evaluation times corroborates Cr(VI) as a genotoxic agent and demonstrates that both clastogenic and aneugenic damages are involved in the formation of MN. |
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MeSH term(s) | Female ; Animals ; Mice ; Mutagens ; Micronucleus Tests ; In Situ Hybridization, Fluorescence ; Aneugens ; Mice, Inbred ICR ; DNA Damage ; DNA |
Chemical Substances | Mutagens ; chromium hexavalent ion (18540-29-9) ; Aneugens ; DNA (9007-49-2) |
Language | English |
Publishing date | 2023-06-27 |
Publishing country | Greece |
Document type | Journal Article |
ZDB-ID | 807031-3 |
ISSN | 1791-7549 ; 0258-851X |
ISSN (online) | 1791-7549 |
ISSN | 0258-851X |
DOI | 10.21873/invivo.13252 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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