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Article ; Online: Climatic and calf-related risk factors associated with failure of transfer of passive immunity in Holstein calves in a hot environment.

Mellado, M / Arroyo, N / García, J E / Arias, N / Macías-Cruz, U / Mellado, J

2024 Volume 56, Issue 2, Page(s) 57

Abstract: This retrospective observational study aimed to assess the effect of temperature-humidity index (THI) at calving and in the last trimester of pregnancy and calve-related factors affecting passive transfer of maternal immunoglobulin using Brix ... ...

Abstract	This retrospective observational study aimed to assess the effect of temperature-humidity index (THI) at calving and in the last trimester of pregnancy and calve-related factors affecting passive transfer of maternal immunoglobulin using Brix refractometry in Holstein calves. Blood samples from 4411 Holstein calves from a single large dairy farm in spring 2022 were used. A subset of data containing 6318 calvings was used to determine the effect of climatic conditions on the occurrence of agammaglobulinemia. Risk factors predictive of failure of passive transfer (FPT) were calculated using multiple logistic models. Females were 1.4 times more likely not to have FPT (56%; Brix% > 8 equivalent to ≥ 10 g/L IgG) than males (47.2%). Calves born as singles increased the likelihood of not presenting FPT (52.6%) than calves born as twins (42.9%). Calves from cows with no dystocic delivery had a lower risk for FPT (odds ratio = 2.3) than calves from cows with dystocia. Agammaglobulinemia was 1.5 and 1.8 times more likely to occur in calves with THI ≥ 80 and ≥ 82 in the last trimester of gestation and at calving, respectively, than in calves not experiencing heat stress. Agammaglobulinemia was twice as likely to occur in male than in female calves. Calves with birth weight ≥ 37 kg and gestation length ≥ 275 kg were less likely to present agammaglobulinemia than lighter calves and calves with shorter gestation periods. This study raises questions for management practices in Holstein calves undergoing in utero heat stress and around calving to avoid agammaglobulinemia.
MeSH term(s)	Animals ; Cattle ; Female ; Male ; Pregnancy ; Agammaglobulinemia/veterinary ; Antibodies ; Birth Weight ; Cattle Diseases ; Dystocia/veterinary ; Risk Factors ; Retrospective Studies
Chemical Substances	Antibodies
Language	English
Publishing date	2024-01-24
Publishing country	United States
Document type	Journal Article ; Observational Study, Veterinary
ZDB-ID	603363-5
ISSN	1573-7438 ; 0049-4747
ISSN (online)	1573-7438
ISSN	0049-4747
DOI	10.1007/s11250-024-03900-8
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Article: Seasonal prevalence of gastrointestinal parasites in desert bighorn sheep (Ovis canadensis) in northern Mexico

García, J.E. / Rodriguez-Huerta, F.A. / Lozano, E.A. / Encina, J. / Mellado, M.

Journal of helminthology. , v. 96

2022

Abstract: This study aimed to describe the shedding pattern of gastrointestinal parasite eggs by a wild population of desert bighorn sheep (DBS; Ovis canadensis) in northern Mexico. Seventy-five fresh faecal samples were collected from the ground in each season ... ...

Abstract	This study aimed to describe the shedding pattern of gastrointestinal parasite eggs by a wild population of desert bighorn sheep (DBS; Ovis canadensis) in northern Mexico. Seventy-five fresh faecal samples were collected from the ground in each season within an hour after being expelled by these animals. The generalized linear mixed model showed that eggs per gram of faeces were highest in winter (577 ± 399) and lowest in summer (260 ± 198). Generalized linear models revealed that Strongyloides spp. predominated during most seasons with a peak in summer (85% of faecal samples analysed) and the absence of this helminth in winter. Nematodirus spp. was another helminth present in three seasons, with the presence of this nematode in 35% of the faecal samples in spring and 0% in summer. Other parasites in DBS faeces included Bunostomun spp., Trichostrongylus spp., Cooperia spp., Mecistocirrus digitatus, Haemonchus contortus, Chabertia ovina and Eimeria ovinoidalis. There were differences among seasons in the percentage of these helminths and coccidia in faecal samples for all these parasites. It was concluded that helminths egg output in DBS in a semi-arid rangeland is lowest in summer and spring and highest in autumn and winter. Furthermore, it was shown that DBS in the study site do not suffer from severe parasite burden. Therefore, this nematode parasite burden is compatible with the conservation and well-being of this particular population.
Keywords	Chabertia ; Cooperia ; Eimeria ; Haemonchus contortus ; Nematodirus ; Ovis canadensis ; Strongyloides ; Trichostrongylus ; autumn ; eggs ; feces ; gastrointestinal parasites ; gastrointestinal system ; helminths ; rangelands ; spring ; summer ; winter ; Mexico
Language	English
Publishing place	Cambridge University Press
Document type	Article
ZDB-ID	390188-9
ISSN	1475-2697 ; 0022-149X
ISSN (online)	1475-2697
ISSN	0022-149X
DOI	10.1017/S0022149X22000207
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Article ; Online: Flower colour and size signals differ depending on geographical location and altitude region.

Garcia, J E / Dyer, A G / Burd, M / Shrestha, M

Plant biology (Stuttgart, Germany)

2021 Volume 23, Issue 6, Page(s) 905–914

Abstract: Bees are major pollinators of angiosperms and have phylogenetically conserved colour vision but differ in how various key species use achromatic information that is vital for both flower detection and size processing. We modelled green contrast and ... ...

Abstract	Bees are major pollinators of angiosperms and have phylogenetically conserved colour vision but differ in how various key species use achromatic information that is vital for both flower detection and size processing. We modelled green contrast and colour contrast signals from flowers of different countries where there are well established differences in availability of model bee species along altitudinal gradients. We tested for consistency in visual signals as expected from generalization in pollination principles using phylogenetically informed linear models. Patterns of chromatic contrast, achromatic green contrast and flower size differed among the three floras we examined. In Nepal there is a significant positive correlation between flower size and colour contrast in the subalpine region, but a negative correlation at the lower altitudes. At high elevations in Norway, where pollinators other than bees are common, flower size was positively correlated with colour contrast. At low and medium altitudes in Norway and in Australia, we did not observe a significant relationship between size and colour contrast. We thus find that the relationship between size, green and colour contrast cannot be generalized across communities, thus suggesting that flower visual signal adaptations to local pollinators are not limited to chromatic contrast.
MeSH term(s)	Altitude ; Animals ; Bees ; Color ; Flowers ; Magnoliopsida ; Pollination
Language	English
Publishing date	2021-09-21
Publishing country	England
Document type	Journal Article
ZDB-ID	1464075-2
ISSN	1438-8677 ; 1435-8603
ISSN (online)	1438-8677
ISSN	1435-8603
DOI	10.1111/plb.13326
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Article ; Online: RedLabRA; a Spanish Network of Microbiology Laboratories for the Surveillance of Antibiotic Resistant Microorganisms.

Cañada-García, J E / Pérez-Vázquez, M / Oteo-Iglesias, J

Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia

2021 Volume 34 Suppl 1, Page(s) 12–14

Abstract: There is an urgent need to control the clinical and public health impact that antibiotic resistance (AR) causes worldwide. Any measure for its control must be based on an up-to-date and comprehensive knowledge of the situation. However, it is difficult ... ...

Abstract	There is an urgent need to control the clinical and public health impact that antibiotic resistance (AR) causes worldwide. Any measure for its control must be based on an up-to-date and comprehensive knowledge of the situation. However, it is difficult to determine the current dimension of AR because a large part of the available information is based on heterogeneous, insufficiently unified and retrospective data. The integration of genomic information in the surveillance of AR is another important factor for improvement. The Spanish Network of Laboratories for the Surveillance of Resistant Microorganisms (RedLabRA) is a structured network of interconnected microbiology laboratories developed within the Spanish National Plan against Antibiotic Resistance. Its main objective is to support the diagnosis of resistance to antibiotics, integrating molecular characterization in the surveillance.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Drug Resistance, Microbial ; Humans ; Laboratories ; Public Health ; Retrospective Studies
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2021-09-30
Publishing country	Spain
Document type	Journal Article ; Review
ZDB-ID	1018135-0
ISSN	1988-9518 ; 0214-3429
ISSN (online)	1988-9518
ISSN	0214-3429
DOI	10.37201/req/s01.03.2021
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Article ; Online: Seasonal prevalence of gastrointestinal parasites in desert bighorn sheep (

García, J E / Rodriguez-Huerta, F A / Lozano, E A / Encina, J / Mellado, M

Journal of helminthology

2022 Volume 96, Page(s) e26

Abstract	This study aimed to describe the shedding pattern of gastrointestinal parasite eggs by a wild population of desert bighorn sheep (DBS; Ovis canadensis) in northern Mexico. Seventy-five fresh faecal samples were collected from the ground in each season within an hour after being expelled by these animals. The generalized linear mixed model showed that eggs per gram of faeces were highest in winter (577 ± 399) and lowest in summer (260 ± 198). Generalized linear models revealed that Strongyloides spp. predominated during most seasons with a peak in summer (85% of faecal samples analysed) and the absence of this helminth in winter. Nematodirus spp. was another helminth present in three seasons, with the presence of this nematode in 35% of the faecal samples in spring and 0% in summer. Other parasites in DBS faeces included Bunostomun spp., Trichostrongylus spp., Cooperia spp., Mecistocirrus digitatus, Haemonchus contortus, Chabertia ovina and Eimeria ovinoidalis. There were differences among seasons in the percentage of these helminths and coccidia in faecal samples for all these parasites. It was concluded that helminths egg output in DBS in a semi-arid rangeland is lowest in summer and spring and highest in autumn and winter. Furthermore, it was shown that DBS in the study site do not suffer from severe parasite burden. Therefore, this nematode parasite burden is compatible with the conservation and well-being of this particular population.
MeSH term(s)	Animals ; Feces/parasitology ; Helminths ; Intestinal Diseases, Parasitic/epidemiology ; Intestinal Diseases, Parasitic/parasitology ; Intestinal Diseases, Parasitic/veterinary ; Mexico/epidemiology ; Nematoda ; Nematode Infections/parasitology ; Parasite Egg Count ; Parasites ; Prevalence ; Seasons ; Sheep ; Sheep Diseases/epidemiology ; Sheep Diseases/parasitology ; Sheep, Bighorn
Language	English
Publishing date	2022-04-11
Publishing country	England
Document type	Journal Article
ZDB-ID	390188-9
ISSN	1475-2697 ; 0022-149X
ISSN (online)	1475-2697
ISSN	0022-149X
DOI	10.1017/S0022149X22000207
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Book ; Online: Mejoramiento del frijol común por resistencia a Empoasca kraemeri

García, J.E.

2016

Keywords	phaseolus vulgaris ; empoasca kraemeri ; plant breeding ; pest resistance ; genetic resistance ; fitomejoramiento ; resistencia a las plagas ; resistencia genética
Language	Spanish
Publishing date	2016-02-29T17:09:55Z
Publisher	International Center for Tropical Agriculture
Publishing country	fr
Document type	Book ; Online
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Article ; Online: Prospective study of the modified Atkins diet in adult drug-resistant epilepsy: effectiveness, tolerability, and adherence.

Alanis Guevara, M I / García de Alba García, J E / López Alanis, A L / González Ojeda, A / Fuentes Orozco, C

Neurologia

2023

Abstract: Introduction: Drug-resistant epilepsy presents high worldwide prevalence and is difficult to control despite the wide variety of available antiepileptic drugs (AED). The modified Atkins diet (MAD) is an additional treatment alternative. Several studies ... ...

Abstract	Introduction: Drug-resistant epilepsy presents high worldwide prevalence and is difficult to control despite the wide variety of available antiepileptic drugs (AED). The modified Atkins diet (MAD) is an additional treatment alternative. Several studies have addressed the use of the ketogenic diet and MAD in children with drug-resistant epilepsy, but insufficient research has been conducted into adults with the same condition. Objective: To evaluate the effectiveness and tolerability of, and adherence to, the MAD in adults with drug-resistant epilepsy. Material and methods: We conducted a 6-month pre-post prospective study at a reference hospital. Patients were prescribed the MAD with limited carbohydrate intake and unlimited fat intake. We conducted clinical and electroencephalographic follow-up according to the relevant guidelines, and assessed adverse effects changes in laboratory findings, and adherence. Results: Thirty-two patients with drug-resistant epilepsy were included in the study. Patients' mean age was 30 years, mean disease progression time was 22 years, and all patients had focal or multifocal epilepsy. Thirty-four percent of patients presented > 50% decreases in overall seizure frequency (P =  .001); seizure control was greater in the first month and subsequently declined. These patients presented weight loss (RR: 7.2; 95% CI, 1.3-39.5; P = .02), good to fair adherence only in the first and third months (RR: 9.4; 95% CI, 0.9-93.6; P = .04 and RR: 0.4; 95% CI, 0.30-0.69; P = .02, respectively). Tolerability data showed that the MAD is safe: adverse effects were minor and short-lived in most cases, with the exception of mild to moderate hyperlipidaemia in one-third of patients. The adherence rate was 50% at the end of the study. Conclusions: In adults with drug-resistant focal epilepsy, the MAD showed adequate tolerability and moderate but decreasing effectiveness and adherence, probably due to a preference for a carbohydrate-based diet.
Language	English
Publishing date	2023-04-28
Publishing country	Spain
Document type	Journal Article
ZDB-ID	2654369-2
ISSN	2173-5808 ; 2173-5808
ISSN (online)	2173-5808
ISSN	2173-5808
DOI	10.1016/j.nrleng.2021.10.008
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Article ; Online: Phenogenetic profile and agronomic contribution of Azospirillum argentinense Az39

Maroniche, G A / Puente, M L / García, J E / Mongiardini, E / Coniglio, A / Nievas, S / Labarthe, M M / Wisniewski-Dyé, F / Rodriguez Cáceres, E / Díaz-Zorita, M / Cassán, F

Microbiological research

2024 Volume 283, Page(s) 127650

Abstract: Azospirillum sp. is a plant growth-promoting rhizobacteria largely recognized for its potential to increase the yield of different important crops. In this work, we present a thorough genomic and phenotypic analysis of A. argentinense ... ...

Abstract	Azospirillum sp. is a plant growth-promoting rhizobacteria largely recognized for its potential to increase the yield of different important crops. In this work, we present a thorough genomic and phenotypic analysis of A. argentinense Az39
MeSH term(s)	Azospirillum/physiology ; Indoleacetic Acids/metabolism ; Plant Development ; Carbon ; South America
Chemical Substances	Indoleacetic Acids ; Carbon (7440-44-0)
Language	English
Publishing date	2024-02-15
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1189614-0
ISSN	1618-0623 ; 0944-5013
ISSN (online)	1618-0623
ISSN	0944-5013
DOI	10.1016/j.micres.2024.127650
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Article ; Online: Effect of co-positivity for brucellosis and tuberculosis on milk yield and fertility of Holstein cows.

Mellado, M / Treviño, N / Véliz, F G / Macías-Cruz, U / Avendaño-Reyes, L / de Santiago, A / García, J E

Tropical animal health and production

2021 Volume 53, Issue 5, Page(s) 504

Abstract: This study aimed to determine whether cows detected as tuberculosis (bTB) reactors and seropositive to brucellosis (bBR), as well as co-positive to bBR and bTB (bBR-bTB) and with a complete lactation before slaughter, were associated with reduced milk ... ...

Abstract	This study aimed to determine whether cows detected as tuberculosis (bTB) reactors and seropositive to brucellosis (bBR), as well as co-positive to bBR and bTB (bBR-bTB) and with a complete lactation before slaughter, were associated with reduced milk production and fertility. A total of 8068 productive and reproductive records of high-yielding Holstein cows from a single large dairy herd with a high prevalence of bTB and bBR were collected from 2012 to 2015. Lactation derived either from calving (n = 6019) or hormonally induced lactation (n = 2049), and all cows received growth hormone throughout lactation. For cows not induced into lactation, pregnancy rate to first service for healthy cows (C; 26.6%) was higher (P < 0.01) than bBR (15.2%), bTB (15.8%), and bBR-bTB (1.3%) cows. For induced cows, pregnancy rate to first service did not differ significantly among C, bBR, and bTB (14.5-17.3%) cows, but the percentage success of first service was extremely low (1.3%; P < 0.01) in bBR-bTB cows. Services per pregnancy (only pregnant cows) were lowest for C (3.3 ± 2.9; P < 0.01) and highest (6.4 ± 3.4) for bBR-bTB non-induced cows. This variable was lowest for C (2.9 ± 2.5; P < 0.01) and highest for bBR-bTB non-induced cows (6.3 ± 3.1). Pregnancy rate to all services did not differed for C (79.5%), bBR (76.7%), and bTB (75.9%) but was lower (58.9%; P < 0.01) for bBR-bTB non-induced cows. For induced cows this variable was highest for bBR (53.3%) and lowest for bBR-bTB (34.1%; P < 0.01) non-induced cows. 305-d milk production was increased by 4%, and total milk yield by 7% in TB-positive cows compared to that of the negative cows non-induced hormonally into lactation. This study showed the negative impact of the co-positivity for bTB and bBR on the reproductive efficiency of Holstein cows, although positive bTB and bBR tests enhanced milk yield.
MeSH term(s)	Animals ; Brucellosis, Bovine/diagnosis ; Cattle ; Cattle Diseases/diagnosis ; Female ; Fertility ; Lactation ; Milk ; Pregnancy ; Tuberculosis, Bovine/diagnosis
Language	English
Publishing date	2021-10-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	603363-5
ISSN	1573-7438 ; 0049-4747
ISSN (online)	1573-7438
ISSN	0049-4747
DOI	10.1007/s11250-021-02952-4
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Article ; Online: Localization and survival of Azospirillum brasilense Az39 in soybean leaves.

Puente, M L / Maroniche, G A / Panepucci, M / Sabio Y García, J / García, J E / Criado, M V / Molina, R / Cassán, F

Letters in applied microbiology

2021 Volume 72, Issue 5, Page(s) 626–633

Abstract: In recent years, foliar inoculation has gained acceptance among the available methods to deliver plant beneficial micro-organisms to crops under field conditions. Colonization efficiency by such micro-organisms largely depends on their ability to survive ...

Abstract	In recent years, foliar inoculation has gained acceptance among the available methods to deliver plant beneficial micro-organisms to crops under field conditions. Colonization efficiency by such micro-organisms largely depends on their ability to survive when applied on the leaves. In this work, we evaluated the survival and localization of Azospirillum brasilense Az39 (Az39) in excised soybean leaves. Scanning electron microscopy and confocal laser scanning microscopy of a red fluorescent-transformed variant of Az39 were used to determine bacterial localization, while the most probable number and plate count methods were applied for bacterial quantification. Microscopic observations indicated a decrease in the number of Az39 cells on the leaf surface at 24 h after treatment, whereas midribs and cell-cell junctions of the inner leaf epidermis became highly populated zones. The presence of Az39 inside xylem vessels was corroborated at 6 h after bacterization. Az39 population did not significantly decrease throughout 24 h. We could visualize Az39 cells on the surface and in internal tissues of soybean leaves and recover them through culture methodologies. These results evidence the survival capacity of Az39 on and inside leaves and suggest a previously unnoticed endophytic potential for this well-known plant growth-promoting rhizobacteria strain.
MeSH term(s)	Azospirillum brasilense/growth & development ; Microscopy, Confocal ; Microscopy, Electron, Scanning ; Plant Leaves/microbiology ; Plant Roots/microbiology ; Glycine max/microbiology
Language	English
Publishing date	2021-02-19
Publishing country	England
Document type	Journal Article
ZDB-ID	632584-1
ISSN	1472-765X ; 0266-8254
ISSN (online)	1472-765X
ISSN	0266-8254
DOI	10.1111/lam.13444
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