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  1. Article ; Online: DhDIT2

    Padilla-Garfias, Francisco / Sánchez, Norma Silvia / Calahorra, Martha / Peña, Antonio

    Journal of fungi (Basel, Switzerland)

    2022  Volume 8, Issue 11

    Abstract: Pollutants, such as polycyclic aromatic hydrocarbons (PAHs), e.g., benzo(a)pyrene (BaP), are common components of contaminating mixtures. Such compounds are ubiquitous, extremely toxic, and they pollute soils and aquatic niches. The need for new ... ...

    Abstract Pollutants, such as polycyclic aromatic hydrocarbons (PAHs), e.g., benzo(a)pyrene (BaP), are common components of contaminating mixtures. Such compounds are ubiquitous, extremely toxic, and they pollute soils and aquatic niches. The need for new microorganism-based remediation strategies prompted researchers to identify the most suitable organisms to eliminate pollutants without interfering with the ecosystem. We analyzed the effect caused by BaP on the growth properties of Candida albicans, Debaryomyces hansenii, Rhodotorula mucilaginosa, and Saccharomyces cerevisiae. Their ability to metabolize BaP was also evaluated. The aim was to identify an optimal candidate to be used as the central component of a mycoremediation strategy. The results show that all four yeast species metabolized BaP by more than 70%, whereas their viability was not affected. The best results were observed for D. hansenii. When an incubation was performed in the presence of a cytochrome P450 (CYP) inhibitor, no BaP degradation was observed. Thus, the initial oxidation step is mediated by a CYP enzyme. Additionally, this study identified the D. hansenii DhDIT2 gene as essential to perform the initial degradation of BaP. Hence, we propose that D. hansenii and a S. cerevisiae expressing the DhDIT2 gene are suitable candidates to degrade BaP in contaminated environments.
    Language English
    Publishing date 2022-10-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2784229-0
    ISSN 2309-608X ; 2309-608X
    ISSN (online) 2309-608X
    ISSN 2309-608X
    DOI 10.3390/jof8111150
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Study of the mechanism of ε-poly-l-lysine as an antifungal on Candida albicans and Saccharomyces cerevisiae

    Padilla-Garfias, Francisco / Ríos-Cifuentes, Laura / Sánchez, Norma Silvia / Calahorra, Martha / Peña, Antonio

    Biochimica et biophysica acta. 2022 Oct., v. 1866, no. 10

    2022  

    Abstract: The antimicrobial activity of ε-poly-l-lysine (EPL) has been documented, but its antifungal activity on yeast is not well defined and its mechanism of action has been vaguely explained. Our studies revealed that on both, Candida albicans and ... ...

    Abstract The antimicrobial activity of ε-poly-l-lysine (EPL) has been documented, but its antifungal activity on yeast is not well defined and its mechanism of action has been vaguely explained. Our studies revealed that on both, Candida albicans and Saccharomyces cerevisiae, the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) were 250 μg·mL⁻¹; EPL produced a K⁺ and Ca²⁺ efflux, and at higher concentrations also an efflux of material absorbing at 260 nm, small peptides, and phosphate is produced, along with the inhibition of fermentation and extracellular acidification and respiration. Moreover, growth was inhibited, reactive oxygen species (ROS) production increased, and cell viability decreased. The polycation also produced plasma membrane potential hyperpolarization. The effects were dependent both on the cell quantity and polycation concentration, as well as the media used. The plasma membrane disruption was confirmed by TEM and PI staining.
    Keywords Candida albicans ; Saccharomyces cerevisiae ; acidification ; antifungal properties ; calcium ; cations ; cell viability ; fermentation ; mechanism of action ; membrane potential ; minimum inhibitory concentration ; peptides ; phosphates ; plasma membrane ; reactive oxygen species ; yeasts
    Language English
    Dates of publication 2022-10
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 840755-1
    ISSN 0304-4165
    ISSN 0304-4165
    DOI 10.1016/j.bbagen.2022.130197
    Database NAL-Catalogue (AGRICOLA)

    Full text online

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    In stock of ZB MED Cologne/Königswinter

    Uc I Zs.247: Show issues Location:
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  3. Article: Can You Hear Me Now? How Communication Technology Affects Protest and Repression.

    Christensen, Darin / Garfias, Francisco

    Quarterly journal of political science

    2018  Volume 13, Issue 1, Page(s) 89–117

    Abstract: Commentators covering recent social movements, such as the Arab Spring, commonly claim that cell phones enable protests. Yet, existing empirical work does not conclusively support this contention: some studies find that these technologies actually reduce ...

    Abstract Commentators covering recent social movements, such as the Arab Spring, commonly claim that cell phones enable protests. Yet, existing empirical work does not conclusively support this contention: some studies find that these technologies actually reduce collective action; many others struggle to overcome the selection problems that dog observational research. We propose two mechanisms through which cell phones affect protests: (1) by enabling communication among would-be protesters, cell phones lower coordination costs; and (2) these technologies broadcast information about whether a protest is repressed. Knowing that a larger audience now witnesses and may be angered by repression, governments refrain from squashing demonstrations, further lowering the cost of protesting. We evaluate these mechanisms using high-resolution global data on the expansion of cell phone coverage and incidence of protest from 2007 to 2014. Our difference-in-differences estimates indicate that cell phone coverage increases the probability of protest by over half the mean. Consistent with our second mechanism, we also find that gaining coverage has a larger effect when it connects a locality to a large proportion of other citizens.
    Language English
    Publishing date 2018-03-29
    Publishing country United States
    Document type Journal Article
    ISSN 1554-0626
    ISSN 1554-0626
    DOI 10.1561/100.00016129
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Study of the mechanism of ε-poly-l-lysine as an antifungal on Candida albicans and Saccharomyces cerevisiae.

    Padilla-Garfias, Francisco / Ríos-Cifuentes, Laura / Sánchez, Norma Silvia / Calahorra, Martha / Peña, Antonio

    Biochimica et biophysica acta. General subjects

    2022  Volume 1866, Issue 10, Page(s) 130197

    Abstract: The antimicrobial activity of ε-poly-l-lysine (EPL) has been documented, but its antifungal activity on yeast is not well defined and its mechanism of action has been vaguely explained. Our studies revealed that on both, Candida albicans and ... ...

    Abstract The antimicrobial activity of ε-poly-l-lysine (EPL) has been documented, but its antifungal activity on yeast is not well defined and its mechanism of action has been vaguely explained. Our studies revealed that on both, Candida albicans and Saccharomyces cerevisiae, the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) were 250 μg·mL
    MeSH term(s) Antifungal Agents/metabolism ; Antifungal Agents/pharmacology ; Candida albicans/metabolism ; Microbial Sensitivity Tests ; Polylysine/metabolism ; Polylysine/pharmacology ; Saccharomyces cerevisiae/metabolism
    Chemical Substances Antifungal Agents ; Polylysine (25104-18-1)
    Language English
    Publishing date 2022-06-19
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 60-7
    ISSN 1872-8006 ; 1879-2596 ; 1879-260X ; 1879-2642 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    ISSN (online) 1872-8006 ; 1879-2596 ; 1879-260X ; 1879-2642 ; 1879-2618 ; 1879-2650
    ISSN 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    DOI 10.1016/j.bbagen.2022.130197
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  5. Article ; Online: The Use of Thioflavin T for the Estimation and Measurement of the Plasma Membrane Electric Potential Difference in Different Yeast Strains.

    Peña, Antonio / Sánchez, Norma Silvia / Padilla-Garfias, Francisco / Ramiro-Cortés, Yazmín / Araiza-Villanueva, Minerva / Calahorra, Martha

    Journal of fungi (Basel, Switzerland)

    2023  Volume 9, Issue 9

    Abstract: The use of the cationic, dye thioflavin T (ThT), to estimate the electric plasma membrane potential difference (PMP) via the fluorescence changes and to obtain its actual values from the accumulation of the dye, considering important correction factors ... ...

    Abstract The use of the cationic, dye thioflavin T (ThT), to estimate the electric plasma membrane potential difference (PMP) via the fluorescence changes and to obtain its actual values from the accumulation of the dye, considering important correction factors by its binding to the internal components of the cell, was described previously for baker's yeast. However, it was considered important to explore whether the method developed could be applied to other yeast strains. Alternative ways to estimate the PMP by using flow cytometry and a multi-well plate reader are also presented here. The methods were tested with other strains of
    Language English
    Publishing date 2023-09-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2784229-0
    ISSN 2309-608X ; 2309-608X
    ISSN (online) 2309-608X
    ISSN 2309-608X
    DOI 10.3390/jof9090948
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Serratia fonticola

    Villasuso-Alcocer, Victor / Flores-Tapia, Juan P / Perez-Garfias, Fernando / Rochel-Perez, Andrea / Mendez-Dominguez, Nina

    World journal of clinical cases

    2022  Volume 10, Issue 29, Page(s) 10600–10605

    Abstract: Background: Diagnosis of emphysematous pyelonephritis has been described around the world for some decades, frequently associated with : Case summary: A 38-year-old female presented with fever, severe pain in the right flank and changes in urinary ... ...

    Abstract Background: Diagnosis of emphysematous pyelonephritis has been described around the world for some decades, frequently associated with
    Case summary: A 38-year-old female presented with fever, severe pain in the right flank and changes in urinary habits. She was admitted, and emphysematous pyelonephritis was confirmed by an abdominal computerized tomography and urine cultures; the latter showed
    Conclusion: Emphysematous pyelonephritis infections in non-diabetic patients are rare but can be severe and life-threatening. This case suggests that
    Language English
    Publishing date 2022-10-25
    Publishing country United States
    Document type Case Reports
    ISSN 2307-8960
    ISSN 2307-8960
    DOI 10.12998/wjcc.v10.i29.10600
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Disfunción de la proteína surfactante C en pacientes pediátricos: Caso Clínico.

    Nuñez-Paucar, Héctor / Valera-Moreno, Carlos / Atamari-Anahui, Noé / Zamudio-Aquise, Mariela Katherine / Torres-Salas, Juan Carlos / Lipa-Chancolla, Roxana / Pérez-Garfias, Fabio / Gómez-Ponce, Víctor / Untiveros-Tello, Alex

    Andes pediatrica : revista Chilena de pediatria

    2023  Volume 93, Issue 5, Page(s) 733–740

    Abstract: Pulmonary surfactant dysfunction disorders are caused by genetic defects that alter pulmonary surfactant metabolism. They are rare disorders and cause significant morbidity and mortality in the neonatal and pediatric populations.: Objective: To ... ...

    Title translation Surfactant protein C dysfunction in pediatric patients: Clinical Case.
    Abstract Pulmonary surfactant dysfunction disorders are caused by genetic defects that alter pulmonary surfactant metabolism. They are rare disorders and cause significant morbidity and mortality in the neonatal and pediatric populations.
    Objective: To describe the clinical, histopathological, and ultrastructural findings of the lamellar body that suggest surfactant protein C (SP-C) dysfunction, where confirmatory genetic studies are not available.
    Clinical case: We report three pediatric cases of pul monary surfactant dysfunction disorders from a pediatric hospital in Peru. Video-assisted lung biop sy was performed in all cases. Ultrastructural studies of the lamellar body were compatible with type- C pulmonary surfactant dysfunction. The treatment used was methylprednisolone pulses monthly for six months, then every two months, varying the duration according to the clinical evolution. They also received daily hydroxychloroquine and azithromycin three times a week. Clinical evaluations, eye fundus, echocardiogram, electrocardiogram, and biochemistry were performed periodically. At follow-up, there was a good response to treatment and no adverse effects were observed. One case died despite the therapies received.
    Conclusions: In 3 patients with type-C surfactant dysfunction, treatment with corticosteroids, hydroxychloroquine, and azithromycin was successful in 2 of them. This is one of the first case series reported in Peru that contributes to the study of these diseases, es pecially in low- and medium-income countries.
    MeSH term(s) Child ; Humans ; Azithromycin ; Hydroxychloroquine/therapeutic use ; Protein C/genetics ; Pulmonary Surfactant-Associated Protein C/genetics ; Surface-Active Agents
    Chemical Substances Azithromycin (83905-01-5) ; Hydroxychloroquine (4QWG6N8QKH) ; Protein C ; Pulmonary Surfactant-Associated Protein C ; Surface-Active Agents
    Language Spanish
    Publishing date 2023-11-01
    Publishing country Chile
    Document type Case Reports ; English Abstract ; Journal Article
    ISSN 2452-6053
    ISSN (online) 2452-6053
    DOI 10.32641/andespediatr.v93i5.3873
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Disfunción del transportador del surfactante pulmonar ABCA3: reporte de un caso peruano.

    Nuñez-Paucar, Héctor / Valera-Moreno, Carlos / Zamudio-Aquise, Mariela K / Lipa-Chancolla, Roxana / Pérez-Garfias, Fabio / Moncada-Arias, Ana G / Atamari-Anahui, Noé

    Boletin medico del Hospital Infantil de Mexico

    2021  Volume 78, Issue 3

    Title translation Surfactant ABCA3 transporter dysfunction: a case report from Peru.
    MeSH term(s) ATP-Binding Cassette Transporters/genetics ; Humans ; Lung Diseases, Interstitial ; Peru ; Surface-Active Agents
    Chemical Substances ABCA3 protein, human ; ATP-Binding Cassette Transporters ; Surface-Active Agents
    Language Esperanto
    Publishing date 2021-01-12
    Publishing country Mexico
    Document type Case Reports ; Journal Article
    ZDB-ID 730519-9
    ISSN 1665-1146 ; 1665-1146 ; 0539-6115 ; 0539-6123
    ISSN (online) 1665-1146
    ISSN 1665-1146 ; 0539-6115 ; 0539-6123
    DOI 10.24875/BMHIM.20000123
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 258: Show issues Location:
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  9. Book: Cincuenta años

    Garfias, Francisco

    antología poética (1942 - 1992)

    (Colección Enebro ; 5)

    1996  

    Author's details Francisco Garfias
    Series title Colección Enebro ; 5
    Language Spanish
    Size 415 S, 1 Portr, 23 cm
    Publisher Diputación Provincial
    Publishing place Huelva
    Document type Book
    Note Includes bibliographical references (p. 411-415)
    ISBN 8481630780 ; 9788481630787
    Database Former special subject collection: coastal and deep sea fishing

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  10. Article ; Online: Actinomicosis pulmonar en niños: reporte de dos casos peruanos.

    Nuñez-Paucar, Héctor / Valera-Moreno, Carlos / Zamudio-Aquise, Mariela Katherine / Pérez-Garfias, Fabio / Guzmán-Quispe, Eduardo Jesús / Calizaya-Gallegos, Carlo / Ccorahua-Rios, Maycol Suker / Santander-Cahuantico, Ana Claudia / Ordoñez-Linares, Marco Edmundo / Atamari-Anahui, Noé

    Medwave

    2020  Volume 20, Issue 7, Page(s) e7986

    Abstract: Introduction: Actinomycosis is a rare infectious disease caused by Gram-positive bacteria. The most common species is Actinomyces israelii. Among its forms of presentation, the thoracic is the least frequent.: Case presentation: We report two ... ...

    Title translation Pulmonary actinomycosis in children: report of two Peruvian cases.
    Abstract Introduction: Actinomycosis is a rare infectious disease caused by Gram-positive bacteria. The most common species is Actinomyces israelii. Among its forms of presentation, the thoracic is the least frequent.
    Case presentation: We report two patients with thoracic actinomycosis, 8 and 13 years old, from different geographical areas of Peru. The first case had empyema necessitans and the second, lung consolidation and recurrent hemoptysis. Both had a certain degree of difficulty in their diagnosis but responded favorably to antibiotics and surgical treatment. The diagnosis was based on the histopathological study. However, we were not able to ascertain the species of actinomyces.
    Conclusion: Thoracic actinomycosis is rare in children and presents as a parenchymal lesion with possible fistulization to the chest wall. This article is one of the few in the Peruvian literature, constituting a contribution to the knowledge of the disease and its management in pediatrics.
    MeSH term(s) Actinomyces/isolation & purification ; Actinomycosis/diagnosis ; Actinomycosis/microbiology ; Actinomycosis/therapy ; Adolescent ; Anti-Bacterial Agents/administration & dosage ; Child ; Female ; Humans ; Lung Diseases/diagnosis ; Lung Diseases/microbiology ; Lung Diseases/therapy ; Male ; Peru
    Chemical Substances Anti-Bacterial Agents
    Language Spanish
    Publishing date 2020-08-06
    Publishing country Chile
    Document type Case Reports ; Journal Article
    ZDB-ID 2818022-7
    ISSN 0717-6384 ; 0717-6384
    ISSN (online) 0717-6384
    ISSN 0717-6384
    DOI 10.5867/medwave.2020.07.7986
    Database MEDical Literature Analysis and Retrieval System OnLINE

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