Article ; Online: Design of optoelectrodes for the remote imaging of cells and in situ electrochemical detection of neurosecretory events.
Bioelectrochemistry (Amsterdam, Netherlands)
2022 Volume 148, Page(s) 108262
Abstract: Optical fibers have opened avenues for remote imaging, bioanalyses and recently optogenetics. Besides, miniaturized electrochemical sensors have offered new opportunities in sensing directly redox neurotransmitters. The combination of both optical and ... ...
| Abstract | Optical fibers have opened avenues for remote imaging, bioanalyses and recently optogenetics. Besides, miniaturized electrochemical sensors have offered new opportunities in sensing directly redox neurotransmitters. The combination of both optical and electrochemical approaches was usually performed on the platform of microscopes or within microsystems. In this work, we developed optoelectrodes which features merge the advantages of both optical fibers and microelectrodes. Optical fiber bundles were modified at one of their extremity by a transparent ITO deposit. The electrochemical responses of these ITO-modified bundles were characterized for the detection of dopamine, epinephrine and norepinephrine. The analytical performances of the optoelectrodes were equivalent to the ones reported for carbon microelectrodes. The remote imaging of model neurosecretory PC12 cells by optoelectrodes was performed upon cell-staining with common fluorescent dyes: acridine orange and calcein-AM. An optoelectrode placed by micromanipulation at a few micrometers-distance from the cells offered remote images with single cell resolution. Finally, in situ electrochemical sensing was demonstrated by additions of K |
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| MeSH term(s) | Acridine Orange ; Animals ; Carbon ; Dopamine/analysis ; Epinephrine ; Fluorescent Dyes ; Microelectrodes ; Neurotransmitter Agents ; Norepinephrine ; Rats ; Secretagogues |
| Chemical Substances | Fluorescent Dyes ; Neurotransmitter Agents ; Secretagogues ; Carbon (7440-44-0) ; Acridine Orange (F30N4O6XVV) ; Dopamine (VTD58H1Z2X) ; Norepinephrine (X4W3ENH1CV) ; Epinephrine (YKH834O4BH) |
| Language | English |
| Publishing date | 2022-09-13 |
| Publishing country | Netherlands |
| Document type | Journal Article |
| ZDB-ID | 2010650-6 |
| ISSN | 1878-562X ; 0302-4598 ; 1567-5394 |
| ISSN (online) | 1878-562X |
| ISSN | 0302-4598 ; 1567-5394 |
| DOI | 10.1016/j.bioelechem.2022.108262 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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