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Article ; Online: Measurement of gravitational and thermal effects in a liquid-actuated torsion pendulum.

Allocca, Annalisa / Bassan, Massimo / De Laurentis, Martina / De Rosa, Rosario / Di Fiore, Luciano / D'Onofrio, Luca / Errico, Luciano / Garufi, Fabio / Grado, Aniello / Hoyle, C D / Lucchesi, David / Minenkov, Yury / Passeggio, Giuseppe / Pucacco, Giuseppe / Sequino, Valeria / Tarallo, Oreste / Trozzo, Lucia / Visco, Massimo

The Review of scientific instruments

2023 Volume 94, Issue 11

Abstract: We describe a proof-of-principle experiment aiming to investigate the inverse-square law of gravitation at the centimeter scale. The sensor is a two-stage torsion pendulum, while actuation is accomplished by a variable liquid mass. The time-varying ... ...

Abstract	We describe a proof-of-principle experiment aiming to investigate the inverse-square law of gravitation at the centimeter scale. The sensor is a two-stage torsion pendulum, while actuation is accomplished by a variable liquid mass. The time-varying gravitational force is related to the level of the circulating fluid in one or two containers at a short distance from the test mass, with all moving mechanical parts positioned at a large distance. We provide a description of the apparatus and present the first results. We identified a systematic effect of thermal origin, producing offsets of few fNm in torque and of about 10 pN in force. When this effect is neutralized, the measurements agree well with the predictions of simulations. We also discuss the upcoming instrument upgradations and the expected sensitivity improvement that will allow us to perform measurements with adequate accuracy to investigate the unexplored regions of the α-λ parameter space of a Yukawa-like deviation from the Newtonian potential.
Language	English
Publishing date	2023-11-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	209865-9
ISSN	1089-7623 ; 0034-6748
ISSN (online)	1089-7623
ISSN	0034-6748
DOI	10.1063/5.0162604
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Article: Effect of hyaluronic acid and polysaccharides from Opuntia ficus indica (L.) cladodes on the metabolism of human chondrocyte cultures.

Panico, A M / Cardile, V / Garufi, F / Puglia, C / Bonina, F / Ronsisvalle, S

Journal of ethnopharmacology

2007 Volume 111, Issue 2, Page(s) 315–321

Abstract: Conventional medications in articular disease are often effective for symptom relief, but they can also cause significant side effects and do not slow the progression of the disease. Several natural substances have been shown to be effective as non- ... ...

Abstract	Conventional medications in articular disease are often effective for symptom relief, but they can also cause significant side effects and do not slow the progression of the disease. Several natural substances have been shown to be effective as non-steroidal anti-inflammatory drugs at relieving the symptoms of osteoarthritis (OA), and preliminary evidence suggests that some of these compounds may exert a favourable influence on the course of the disease. In this study, we assay the anti-inflammatory/chondroprotective effect of some lyophilised extracts obtained from Opuntia ficus indica (L.) cladodes and of hyaluronic acid (HA) on the production of key molecules released during chronic inflammatory events such as nitric oxide (NO), glycosaminoglycans (GAGs), prostaglandins (PGE(2)) and reactive oxygen species (ROS) in human chondrocyte culture, stimulated with proinflammatory cytokine interleukin-1 beta (IL-1 beta). Further the antioxidant effect of these extracts was evaluated in vitro employing the bleaching of the stable 1,1-diphenyl-2-picrylhydrazyl radical (DPPH test). All the extracts tested in this study showed an interesting profile in active compounds. Particularly some of these extracts were characterized by polyphenolic and polysaccharidic species. In vitro results pointed out that the extracts of Opuntia ficus indica cladodes were able to contrast the harmful effects of IL-1 beta. Our data showed the protective effect of the extracts of Opuntia ficus indica cladodes in cartilage alteration, which appears greater than that elicited by hyaluronic acid (HA) commonly employed as visco-supplementation in the treatment of joint diseases.
MeSH term(s)	Anti-Inflammatory Agents/chemistry ; Anti-Inflammatory Agents/pharmacology ; Antioxidants/metabolism ; Cartilage, Articular/cytology ; Cell Culture Techniques ; Cell Survival/drug effects ; Cells, Cultured ; Chondrocytes/cytology ; Chondrocytes/drug effects ; Chondrocytes/metabolism ; Culture Media, Conditioned/chemistry ; Dinoprostone/analysis ; Drug Evaluation, Preclinical ; Femoral Neck Fractures/pathology ; Femoral Neck Fractures/surgery ; Glycosaminoglycans/analysis ; Humans ; Hyaluronic Acid/pharmacology ; Nitrites/analysis ; Opuntia/chemistry ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Plant Stems/chemistry ; Polysaccharides/chemistry ; Polysaccharides/pharmacology ; Reactive Oxygen Species/analysis ; Reactive Oxygen Species/metabolism ; Spectrophotometry/methods
Chemical Substances	Anti-Inflammatory Agents ; Antioxidants ; Culture Media, Conditioned ; Glycosaminoglycans ; Nitrites ; Plant Extracts ; Polysaccharides ; Reactive Oxygen Species ; Hyaluronic Acid (9004-61-9) ; Dinoprostone (K7Q1JQR04M)
Language	English
Publishing date	2007-05-04
Publishing country	Ireland
Document type	Comparative Study ; Evaluation Studies ; Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2006.11.020
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Article ; Online: Approaching Free Fall on Two Degrees of Freedom: Simultaneous Measurement of Residual Force and Torque on a Double Torsion Pendulum.

Bassan, M / Cavalleri, A / De Laurentis, M / De Marchi, F / De Rosa, R / Di Fiore, L / Dolesi, R / Finetti, N / Garufi, F / Grado, A / Hueller, M / Marconi, L / Milano, L / Pucacco, G / Stanga, R / Visco, M / Vitale, S / Weber, W J

Physical review letters

2016 Volume 116, Issue 5, Page(s) 51104

Abstract: A torsion pendulum with 2 soft degrees of freedom (DOFs), realized by off-axis cascading two torsion fibers, has been built and operated. This instrument helps characterize the geodesic motion of a test mass for LISA Pathfinder or any other free-fall ... ...

Abstract	A torsion pendulum with 2 soft degrees of freedom (DOFs), realized by off-axis cascading two torsion fibers, has been built and operated. This instrument helps characterize the geodesic motion of a test mass for LISA Pathfinder or any other free-fall space mission, providing information on cross talk and other effects that cannot be detected when monitoring a single DOF. We show that it is possible to simultaneously measure both the residual force and the residual torque acting on a quasifree test mass. As an example of the investigations that a double pendulum allows, we report the measurement of the force-to-torque cross talk, i.e., the amount of actuation signal, produced by applying a force on the suspended test mass, that leaks into the rotational DOF, detected by measuring the corresponding (unwanted) torque.
Language	English
Publishing date	2016-02-05
Publishing country	United States
Document type	Journal Article
ZDB-ID	208853-8
ISSN	1079-7114 ; 0031-9007
ISSN (online)	1079-7114
ISSN	0031-9007
DOI	10.1103/PhysRevLett.116.051104
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Article: "In vitro" differences among (R) and (S) enantiomers of profens in their activities related to articular pathophysiology.

Panico, A M / Cardile, V / Gentile, B / Garufi, F / Avondo, S / Ronsisvalle, S

Inflammation

2005 Volume 29, Issue 4-6, Page(s) 119–128

Abstract: An important group of non steroidal antinflammatory drugs (NSAIDs), which have been used for the symptomatic treatment of various forms of arthritis, are the 2-arylpropionic acid derivatives, 'profens'. By virtue of a chiral carbon atom on the propionic ... ...

Abstract	An important group of non steroidal antinflammatory drugs (NSAIDs), which have been used for the symptomatic treatment of various forms of arthritis, are the 2-arylpropionic acid derivatives, 'profens'. By virtue of a chiral carbon atom on the propionic acid side chain, they exist as enantiomeric pairs. Whereas the S (+) enantiomer could be represented as an effective, but unselective COX inhibitor, the R (-) enantiomer could be much less active in this respect. However, recent findings suggest that certain pharmacological effects of profens cannot be attributed exclusively to the S (+) enantiomer. To obtain further insights into the pharmacological effects of profens, this study investigated the influence of pure enantiomers (S), (R), and racemic flurbiprofen and ketoprofen on the production of NO, MMP-3, PGE(2), ROS and GAGs, key molecules involved in cartilage destruction. Our results show that (S) flurbiprofen and ketoprofen decrease, at 1- and 10-microM concentrations, the interleukin-1beta induced cartilage destruction.
MeSH term(s)	Anti-Inflammatory Agents, Non-Steroidal/chemistry ; Anti-Inflammatory Agents, Non-Steroidal/pharmacology ; Cartilage, Articular/cytology ; Cartilage, Articular/drug effects ; Cartilage, Articular/metabolism ; Cartilage, Articular/physiology ; Cell Survival ; Cells, Cultured ; Chondrocytes/drug effects ; Chondrocytes/metabolism ; Chondrocytes/physiology ; Dinoprostone/metabolism ; Femur Head ; Flurbiprofen/chemistry ; Flurbiprofen/pharmacology ; Glycosaminoglycans/metabolism ; Humans ; Interleukin-1beta/pharmacology ; Ketoprofen/chemistry ; Ketoprofen/pharmacology ; Matrix Metalloproteinase 3/metabolism ; Nitrites/metabolism ; Reactive Oxygen Species ; Stereoisomerism
Chemical Substances	Anti-Inflammatory Agents, Non-Steroidal ; Glycosaminoglycans ; Interleukin-1beta ; Nitrites ; Reactive Oxygen Species ; Flurbiprofen (5GRO578KLP) ; Ketoprofen (90Y4QC304K) ; Matrix Metalloproteinase 3 (EC 3.4.24.17) ; Dinoprostone (K7Q1JQR04M)
Language	English
Publishing date	2005-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	434408-x
ISSN	0360-3997
ISSN	0360-3997
DOI	10.1007/s10753-006-9003-1
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Article: Protective effect of Capparis spinosa on chondrocytes.

Panico, A M / Cardile, V / Garufi, F / Puglia, C / Bonina, F / Ronsisvalle, G

Life sciences

2005 Volume 77, Issue 20, Page(s) 2479–2488

Abstract: The aim of the present study was to evaluate the in vitro chondroprotective effects of the lyophilised methanolic extract from flowering buds of Capparis Spinosa L (LECS). This plant, common to the Mediterranean basin, has been used by the traditional ... ...

Abstract	The aim of the present study was to evaluate the in vitro chondroprotective effects of the lyophilised methanolic extract from flowering buds of Capparis Spinosa L (LECS). This plant, common to the Mediterranean basin, has been used by the traditional medicine for its diuretic and antihypertensive effects and also in certain pathological conditions related to uncontrolled lipid peroxidation. The extract contains many constituents, in particular some flavonoids (kaempferol and quercetin derivatives) and hydrocinammic acids with several known biological effects such as the anti-inflammatory and the antioxidant ones. In this study, we assayed the effect of LECS on human chondrocytes cultures stimulated by proinflammatory cytokine interleukin-1beta (IL-1beta) and we determined the production of key molecules released during chronic inflammatory events (nitric oxide, glycosaminoglycans, prostaglandins and reactive oxygen species). We observed that LECS was able to counteract the harmful effects induced by IL-1beta. This protection appeared to be greater than that elicited by indomethacin, which is usually employed in joint diseases. Since LECS possess a chondroprotective effect, it might be used in the management of cartilage damage during the inflammatory processes.
MeSH term(s)	Anti-Inflammatory Agents, Non-Steroidal/pharmacology ; Capparis/chemistry ; Cell Survival/drug effects ; Cells, Cultured ; Chondrocytes/drug effects ; Chondrocytes/metabolism ; Dinoprostone/metabolism ; Flowers/chemistry ; Glycosaminoglycans/metabolism ; Humans ; Interleukin-1/toxicity ; Nitric Oxide/metabolism ; Plant Extracts/pharmacology ; Reactive Oxygen Species/metabolism
Chemical Substances	Anti-Inflammatory Agents, Non-Steroidal ; Glycosaminoglycans ; Interleukin-1 ; Plant Extracts ; Reactive Oxygen Species ; Nitric Oxide (31C4KY9ESH) ; Dinoprostone (K7Q1JQR04M)
Language	English
Publishing date	2005-09-30
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	3378-9
ISSN	1879-0631 ; 0024-3205
ISSN (online)	1879-0631
ISSN	0024-3205
DOI	10.1016/j.lfs.2004.12.051
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Article: The in vitro effect of a lyophilized extract of wine obtained from Jacquez grapes on human chondrocytes.

Panico, A M / Cardile, V / Avondo, S / Garufi, F / Gentile, B / Puglia, C / Bonina, F / Santagati, N A / Ronsisvalle, G

Phytomedicine : international journal of phytotherapy and phytopharmacology

2006 Volume 13, Issue 7, Page(s) 522–526

Abstract: The present work was aimed at evaluating the in vitro effects of a lyophilized extract of wine (JW-E) obtained from Jacquez grapes (Vitis aestivalis-cinereaxVitis vinifera grapes) on the production of key molecules released in inflammatory disease ... ...

Abstract	The present work was aimed at evaluating the in vitro effects of a lyophilized extract of wine (JW-E) obtained from Jacquez grapes (Vitis aestivalis-cinereaxVitis vinifera grapes) on the production of key molecules released in inflammatory disease utilising interleukin-1beta (IL-1beta) activated chondrocytes. The extract contains large amounts of phenolic components, in particular some flavonoids (flavan-3-ols, also known as catechins) and proanthocyanidins, as hydroxycinnamic acids and anthocyanins, that possess several biological features such as antiinflammatory and antioxidant effects and a "radical scavenger" activity too. In this study, we assayed the effect of JW-E on the production of key molecules released during chronic inflammatory events as nitric oxide (NO), prostaglandins E(2) (PGE(2)) and reactive oxygen species (ROS) in human chondrocytes culture, stimulated with proinflammatory cytokine interleukin-1beta. The JW-E proved to possess good ability against the harmfull effects of IL-1beta. Our data showed the protective effects of JW-E in cartilage alteration, that appears greater than that elicited by indomethacin, a not steroidal antiinflammatory drug (NSAID), commonly employed in joint diseases.
MeSH term(s)	Cartilage, Articular/cytology ; Cells, Cultured ; Chondrocytes/drug effects ; Dinoprostone/metabolism ; Fruit ; Humans ; Inflammation/drug therapy ; Interleukin-1/pharmacology ; Nitric Oxide/metabolism ; Osteochondritis/drug therapy ; Plant Extracts/pharmacology ; Plant Extracts/therapeutic use ; Reactive Oxygen Species/metabolism ; Vitis ; Wine
Chemical Substances	Interleukin-1 ; Plant Extracts ; Reactive Oxygen Species ; Nitric Oxide (31C4KY9ESH) ; Dinoprostone (K7Q1JQR04M)
Language	English
Publishing date	2006-07
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2005.06.009
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Article: Indagine sulla prevalenza della patologia nella Provincia di Palermo.

Pinzone, F / Garufi, F / Gentile, A / Requirez, S

Epidemiologia e prevenzione

1991 Volume 13, Issue 48-49, Page(s) 133–134

Title translation	Prevalence studies on pathology in Palermo Province.
MeSH term(s)	Humans ; Incidence ; Morbidity ; Prevalence ; Rural Population/statistics & numerical data ; Sicily/epidemiology ; Urban Population/statistics & numerical data
Language	Italian
Publishing date	1991-09
Publishing country	Italy
Document type	Journal Article
ZDB-ID	1038112-0
ISSN	1120-9763
ISSN	1120-9763
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Article: Effect of hyaluronic acid and polysaccharides from Opuntia ficus indica (L.) cladodes on the metabolism of human chondrocyte cultures

Panico, A.M / Cardile, V / Garufi, F / Puglia, C / Bonina, F / Ronsisvalle, S

Journal of ethnopharmacology. 2007 May 4, v. 111, issue 2

2007

Abstract	Conventional medications in articular disease are often effective for symptom relief, but they can also cause significant side effects and do not slow the progression of the disease. Several natural substances have been shown to be effective as non-steroidal anti-inflammatory drugs at relieving the symptoms of osteoarthritis (OA), and preliminary evidence suggests that some of these compounds may exert a favourable influence on the course of the disease. In this study, we assay the anti-inflammatory/chondroprotective effect of some lyophilised extracts obtained from Opuntia ficus indica (L.) cladodes and of hyaluronic acid (HA) on the production of key molecules released during chronic inflammatory events such as nitric oxide (NO), glycosaminoglycans (GAGs), prostaglandins (PGE2) and reactive oxygen species (ROS) in human chondrocyte culture, stimulated with proinflammatory cytokine interleukin-1β (IL-1β). Further the antioxidant effect of these extracts was evaluated in vitro employing the bleaching of the stable 1,1-diphenyl-2-picrylhydrazyl radical (DPPH test). All the extracts tested in this study showed an interesting profile in active compounds. Particularly some of these extracts were characterized by polyphenolic and polysaccharidic species. In vitro results pointed out that the extracts of Opuntia ficus indica cladodes were able to contrast the harmful effects of IL-1β. Our data showed the protective effect of the extracts of Opuntia ficus indica cladodes in cartilage alteration, which appears greater than that elicited by hyaluronic acid (HA) commonly employed as visco-supplementation in the treatment of joint diseases.
Keywords	Opuntia ficus-indica ; medicinal plants ; chemical constituents of plants ; hyaluronic acid ; polysaccharides ; plant extracts ; medicinal properties ; bioassays ; human cell lines ; chondrocytes ; metabolism ; inflammation ; anti-inflammatory agents ; antioxidants ; arthritis ; herbal medicines ; traditional medicine
Language	English
Dates of publication	2007-0504
Size	p. 315-321.
Document type	Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2006.11.020
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Benzo[d]isothiazol-3-yl-benzamidines: a class of protective agents on culture of human cartilage and chondrocytes stimulated by IL-1beta.

Vicini, Paola / Incerti, Matteo / Cardile, Venera / Garufi, Floriana / Ronsisvalle, Simone / Panico, Anna Maria

ChemMedChem

2007 Volume 2, Issue 1, Page(s) 113–119

Abstract: New derivatives of N-benzo[d]isothiazol-3-yl-benzamidine 6 a were synthesized as nonacidic anti-inflammatory/antidegenerative agents. We investigated the influence of the amidines 6 a-j on the production of NO, PGE(2), MMP-3, COX-2, ROS, and GAGs, key ... ...

Abstract	New derivatives of N-benzo[d]isothiazol-3-yl-benzamidine 6 a were synthesized as nonacidic anti-inflammatory/antidegenerative agents. We investigated the influence of the amidines 6 a-j on the production of NO, PGE(2), MMP-3, COX-2, ROS, and GAGs, key molecules involved in cartilage destruction in osteoarthritic diseases. The antidegenerative properties of the novel designed derivatives 6 b-j were improved with respect to N-benzo[d]isothiazol-3-yl-benzamidine 6 a. All of the compounds 6 a-j promoted the reduction of most of the IL-1beta-induced harmful effects. Derivatives 6 d, 6 h, and 6 j were the most potent of all the tested compounds, particularly in the human chondrocyte culture model.
MeSH term(s)	Amidines/chemistry ; Anti-Inflammatory Agents/chemical synthesis ; Anti-Inflammatory Agents/pharmacology ; Anti-Inflammatory Agents/therapeutic use ; Benzamidines/chemical synthesis ; Benzamidines/pharmacology ; Cartilage/drug effects ; Cartilage/metabolism ; Cells, Cultured ; Chondrocytes/drug effects ; Chondrocytes/metabolism ; Cyclooxygenase 2/metabolism ; Glycosaminoglycans/metabolism ; Humans ; Interleukin-1beta/pharmacology ; Matrix Metalloproteinase 3/metabolism ; Nitric Oxide/metabolism ; Osteoarthritis/drug therapy ; Protective Agents/chemical synthesis ; Protective Agents/pharmacology ; Protective Agents/therapeutic use ; Reactive Oxygen Species/metabolism ; Structure-Activity Relationship
Chemical Substances	Amidines ; Anti-Inflammatory Agents ; Benzamidines ; Glycosaminoglycans ; Interleukin-1beta ; Protective Agents ; Reactive Oxygen Species ; Nitric Oxide (31C4KY9ESH) ; Cyclooxygenase 2 (EC 1.14.99.1) ; Matrix Metalloproteinase 3 (EC 3.4.24.17)
Language	English
Publishing date	2007-01
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2218496-X
ISSN	1860-7187 ; 1860-7179
ISSN (online)	1860-7187
ISSN	1860-7179
DOI	10.1002/cmdc.200600157
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Article: The in vitro effect of a lyophilized extract of wine obtained from Jacquez grapes on human chondrocytes

Panico, A. M. / Cardile, V. / Avondo, S. / Garufi, F. / Gentile, B. / Puglia, C. / Bonina, F. / Santagati, N. A. / Ronsisvalle, G.

Phytomedicine

2006 Volume 13, Issue 7, Page(s) 522–526

Abstract: Abstract online at publishers website) ...

Institution	Univ. of Catania, Fac. of Pharmacy, Dep. of Pharmaceutical Sciences, 95125 Catania, Italy
Abstract	(Abstract online at publishers website)
Keywords	VARIETY OF VINE ; EXTRACT ; WINE ; PHARMACOLOGY
Language	English
Document type	Article
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2005.06.009
Database	Viticulture and Oenology Abstracts

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