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  1. Article ; Online: Biodegradation of Polystyrene by

    Venegas, Sebastián / Alarcón, Carolina / Araya, Juan / Gatica, Marcell / Morin, Violeta / Tarifeño-Saldivia, Estefanía / Uribe, Elena

    International journal of molecular sciences

    2024  Volume 25, Issue 3

    Abstract: ... Galleria ... ...

    Abstract Galleria mellonella
    MeSH term(s) Animals ; Polystyrenes/metabolism ; Chromatography, Liquid ; Proteomics ; Tandem Mass Spectrometry ; Moths/microbiology ; Larva/metabolism ; Biodegradation, Environmental
    Chemical Substances Polystyrenes
    Language English
    Publishing date 2024-01-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms25031576
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: New Insights into the Determinants of Specificity in Human Type I Arginase: Generation of a Mutant That Is Only Active with Agmatine as Substrate.

    Orellana, María-Soledad / Jaña, Gonzalo A / Figueroa, Maximiliano / Martínez-Oyanedel, José / Medina, Fabiola E / Tarifeño-Saldivia, Estefanía / Gatica, Marcell / García-Robles, María Ángeles / Carvajal, Nelson / Uribe, Elena

    International journal of molecular sciences

    2022  Volume 23, Issue 12

    Abstract: Arginase catalyzes the hydrolysis of L-arginine into L-ornithine and urea. This enzyme has several analogies with agmatinase, which catalyzes the hydrolysis of agmatine into putrescine and urea. However, this contrasts with the highlighted specificity ... ...

    Abstract Arginase catalyzes the hydrolysis of L-arginine into L-ornithine and urea. This enzyme has several analogies with agmatinase, which catalyzes the hydrolysis of agmatine into putrescine and urea. However, this contrasts with the highlighted specificity that each one presents for their respective substrate. A comparison of available crystal structures for arginases reveals an important difference in the extension of two loops located in the entrance of the active site. The first, denominated
    MeSH term(s) Agmatine ; Arginase/metabolism ; Arginine/chemistry ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Humans ; Ornithine ; Substrate Specificity ; Urea
    Chemical Substances Agmatine (70J407ZL5Q) ; Urea (8W8T17847W) ; Arginine (94ZLA3W45F) ; Ornithine (E524N2IXA3) ; Arginase (EC 3.5.3.1)
    Language English
    Publishing date 2022-06-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms23126438
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Therapeutic Use of Vitamin C in Cancer: Physiological Considerations.

    Roa, Francisco J / Peña, Eduardo / Gatica, Marcell / Escobar-Acuña, Kathleen / Saavedra, Paulina / Maldonado, Mafalda / Cuevas, Magdalena E / Moraga-Cid, Gustavo / Rivas, Coralia I / Muñoz-Montesino, Carola

    Frontiers in pharmacology

    2020  Volume 11, Page(s) 211

    Abstract: Since the early studies of William J. McCormick in the 1950s, vitamin C has been proposed as a candidate for the treatment of cancer. A number of reports have shown that pharmacological concentrations of vitamin C selectively kill cancer ... ...

    Abstract Since the early studies of William J. McCormick in the 1950s, vitamin C has been proposed as a candidate for the treatment of cancer. A number of reports have shown that pharmacological concentrations of vitamin C selectively kill cancer cells
    Language English
    Publishing date 2020-03-03
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2020.00211
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Data on SVCT2 transporter expression and localization in cancer cell lines and tissues

    Roa, Francisco J. / Peña, Eduardo / Inostroza, Eveling / Sotomayor, Kirsty / González, Mauricio / Gutierrez-Castro, Francisco A. / Maurin, Michelle / Sweet, Karen / Labrousse, Claire / Gatica, Marcell / Aylwin, Carlos F. / Mendoza, Pamela / Maldonado, Mafalda / Delgado, Carolina / Madariaga, Jaime / Panes, Jessica / Silva-Grecchi, Tiare / Concha, Ilona I. / Moraga-Cid, Gustavo /
    Reyes, Alejandro M. / Muñoz-Montesino, Carola / Vera, Juan Carlos / Rivas, Coralia I.

    Data in Brief. 2019 Aug., v. 25

    2019  

    Abstract: The data presented in this article are related to the research paper entitled “Increased expression of mitochondrial sodium-coupled ascorbic acid transporter-2 (mitSVCT2) as a central feature in breast cancer”, available in Free Radical Biology and ... ...

    Abstract The data presented in this article are related to the research paper entitled “Increased expression of mitochondrial sodium-coupled ascorbic acid transporter-2 (mitSVCT2) as a central feature in breast cancer”, available in Free Radical Biology and Medicine Journal [1]. In this article, we examined the SVCT2 transporter expression in various breast cancer cell lines using RT-PCR and Western blot assays. In addition, we analyzed the subcellular localization of SVCT2 by immunofluorescence colocalization assays and cellular fractionation experiments. Finally, an analysis of different cancer tissue microarrays immunostained for SVCT2 and imaged by The Human Protein Atlas (https://www.proteinatlas.org) is presented.
    Keywords Western blotting ; ascorbic acid ; breast neoplasms ; fluorescent antibody technique ; fractionation ; mitochondria ; neoplasm cells
    Language English
    Dates of publication 2019-08
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 2786545-9
    ISSN 2352-3409
    ISSN 2352-3409
    DOI 10.1016/j.dib.2019.103972
    Database NAL-Catalogue (AGRICOLA)

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  5. Article ; Online: Data on SVCT2 transporter expression and localization in cancer cell lines and tissues.

    Roa, Francisco J / Peña, Eduardo / Inostroza, Eveling / Sotomayor, Kirsty / González, Mauricio / Gutierrez-Castro, Francisco A / Maurin, Michelle / Sweet, Karen / Labrousse, Claire / Gatica, Marcell / Aylwin, Carlos F / Mendoza, Pamela / Maldonado, Mafalda / Delgado, Carolina / Madariaga, Jaime / Panes, Jessica / Silva-Grecchi, Tiare / Concha, Ilona I / Moraga-Cid, Gustavo /
    Reyes, Alejandro M / Muñoz-Montesino, Carola / Vera, Juan Carlos / Rivas, Coralia I

    Data in brief

    2019  Volume 25, Page(s) 103972

    Abstract: The data presented in this article are related to the research paper entitled "Increased expression of mitochondrial sodium-coupled ascorbic acid transporter-2 (mitSVCT2) as a central feature in breast cancer", available in Free Radical Biology and ... ...

    Abstract The data presented in this article are related to the research paper entitled "Increased expression of mitochondrial sodium-coupled ascorbic acid transporter-2 (mitSVCT2) as a central feature in breast cancer", available in Free Radical Biology and Medicine Journal [1]. In this article, we examined the SVCT2 transporter expression in various breast cancer cell lines using RT-PCR and Western blot assays. In addition, we analyzed the subcellular localization of SVCT2 by immunofluorescence colocalization assays and cellular fractionation experiments. Finally, an analysis of different cancer tissue microarrays immunostained for SVCT2 and imaged by The Human Protein Atlas (https://www.proteinatlas.org) is presented.
    Language English
    Publishing date 2019-05-06
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2786545-9
    ISSN 2352-3409 ; 2352-3409
    ISSN (online) 2352-3409
    ISSN 2352-3409
    DOI 10.1016/j.dib.2019.103972
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Increased expression of mitochondrial sodium-coupled ascorbic acid transporter-2 (mitSVCT2) as a central feature in breast cancer.

    Peña, Eduardo / Roa, Francisco J / Inostroza, Eveling / Sotomayor, Kirsty / González, Mauricio / Gutierrez-Castro, Francisco A / Maurin, Michelle / Sweet, Karen / Labrousse, Claire / Gatica, Marcell / Aylwin, Carlos F / Mendoza, Pamela / Maldonado, Mafalda / Delgado, Carolina / Madariaga, Jaime / Panes, Jessica / Silva-Grecchi, Tiare / Concha, Ilona I / Moraga-Cid, Gustavo /
    Reyes, Alejandro M / Muñoz-Montesino, Carola / Vera, Juan Carlos / Rivas, Coralia I

    Free radical biology & medicine

    2019  Volume 135, Page(s) 283–292

    Abstract: The potential role of vitamin C in cancer prevention and treatment remains controversial. While normal human cells obtain vitamin C as ascorbic acid, the prevalent form of vitamin C in vivo, the uptake mechanisms by which cancer cells acquire vitamin C ... ...

    Abstract The potential role of vitamin C in cancer prevention and treatment remains controversial. While normal human cells obtain vitamin C as ascorbic acid, the prevalent form of vitamin C in vivo, the uptake mechanisms by which cancer cells acquire vitamin C has remained unclear. The aim of this study is to characterize how breast cancer cells acquire vitamin C. For this, we determined the expression of vitamin C transporters in normal and breast cancer tissue samples, and in ZR-75, MCF-7, MDA-231 and MDA-468 breast cancer cell lines. At the same time, reduced (AA) and oxidized (DHA) forms of vitamin C uptake experiments were performed in all cell lines. We show here that human breast cancer tissues differentially express a form of SVCT2 transporter, that is systematically absent in normal breast tissues and it is increased in breast tumors. In fact, estrogen receptor negative breast cancer tissue, exhibit the most elevated SVCT2 expression levels. Despite this, our analysis in breast cancer cell lines showed that these cells are not able to uptake ascorbic acid and depend on glucose transporter for the acquisition of vitamin C by a bystander effect. This is consistent with our observations that this form of SVCT2 is completely absent from the plasma membrane and is overexpressed in mitochondria of breast cancer cells, where it mediates ascorbic acid transport. This work shows that breast cancer cells acquire vitamin C in its oxidized form and are capable of accumulated high concentrations of the reduced form. Augmented expression of an SVCT2 mitochondrial form appears to be a common hallmark across all human cancers and might have implications in cancer cells survival capacity against pro-oxidant environments.
    MeSH term(s) Ascorbic Acid/metabolism ; Breast Neoplasms/genetics ; Breast Neoplasms/pathology ; Bystander Effect ; Female ; Gene Expression Regulation, Neoplastic/genetics ; Humans ; MCF-7 Cells ; Mitochondria/genetics ; Mitochondria/pathology ; Mitochondrial Membrane Transport Proteins/genetics ; Oxidation-Reduction ; Reactive Oxygen Species/metabolism ; Sodium/metabolism ; Sodium-Coupled Vitamin C Transporters/genetics
    Chemical Substances Mitochondrial Membrane Transport Proteins ; Reactive Oxygen Species ; SLC23A2 protein, human ; Sodium-Coupled Vitamin C Transporters ; Sodium (9NEZ333N27) ; Ascorbic Acid (PQ6CK8PD0R)
    Language English
    Publishing date 2019-03-19
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 807032-5
    ISSN 1873-4596 ; 0891-5849
    ISSN (online) 1873-4596
    ISSN 0891-5849
    DOI 10.1016/j.freeradbiomed.2019.03.015
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2109: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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  7. Article ; Online: Cis-regulatory elements involved in species-specific transcriptional regulation of the SVCT1 gene in rat and human hepatoma cells.

    Muñoz, Alejandra / Villagrán, Marcelo / Guzmán, Paula / Solíz, Carlos / Gatica, Marcell / Aylwin, Carlos / Sweet, Karen / Maldonado, Mafalda / Escobar, Elizabeth / Reyes, Alejandro M / Toledo, Jorge R / Sánchez, Oliberto / Oñate, Sergio A / Carlos Vera, Juan / Rivas, Coralia I

    Free radical biology & medicine

    2015  Volume 85, Page(s) 183–196

    Abstract: Ascorbic acid is transported into cells by the sodium-coupled vitamin C transporters (SVCTs). Recently, we obtained evidence of differential regulation of SVCT expression in response to acute oxidative stress in cells from species that differ in their ... ...

    Abstract Ascorbic acid is transported into cells by the sodium-coupled vitamin C transporters (SVCTs). Recently, we obtained evidence of differential regulation of SVCT expression in response to acute oxidative stress in cells from species that differ in their capacity to synthesize vitamin C, with a marked decrease in SVCT1 mRNA and protein levels in rat hepatoma cells that was not observed in human hepatoma cells. To better understand the regulatory aspects involved, we performed a structural and functional analysis of the proximal promoter of the SVCT1 rat gene. We cloned a 1476-bp segment containing the proximal promoter of the rat SVCT1 gene and generated deletion-derived truncated promoters of decreasing sizes and mutant promoters by modification of consensus binding sites for transcription factors by site-directed mutagenesis. We next analyzed their capacity to direct the transcription of a reporter gene after transfection into rat H4IIE and human HepG2 hepatoma cells, in experiments involving the coexpression of transcription factors whose consensus binding sequences are present in the SVCT1 promoter. This analysis revealed the presence of two critical cis-regulatory elements of the transcriptional activity of the rat SVCT1 gene promoter, sites containing consensus sequences for the binding of the transcription factors Bach1 and HNF4 that are not present in equivalent locations in the human SVCT1 gene promoter. Moreover, a consensus site for HNF1 that is crucial for the regulation of the human SVCT1 promoter is present in the SVCT1 rat promoter but has no effect on its transcriptional activity. These findings imply that regulation of vitamin C metabolism in the rat, a species with the capacity to synthesize large amounts of ascorbic acid, may differ from that of humans, a species that must obtain ascorbic acid from the diet through a transport mechanism that depends on proper SVCT1 expression.
    MeSH term(s) Animals ; Cell Line, Tumor ; Humans ; Promoter Regions, Genetic ; Rats ; Regulatory Sequences, Nucleic Acid ; Sodium-Coupled Vitamin C Transporters/genetics ; Species Specificity
    Chemical Substances SLC23A1 protein, human ; Slc23a1 protein, rat ; Sodium-Coupled Vitamin C Transporters
    Language English
    Publishing date 2015-08
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 807032-5
    ISSN 1873-4596 ; 0891-5849
    ISSN (online) 1873-4596
    ISSN 0891-5849
    DOI 10.1016/j.freeradbiomed.2015.04.024
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2109: Show issues Location:
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  8. Article ; Online: Sustained blockade of ascorbic acid transport associated with marked SVCT1 loss in rat hepatocytes containing increased ascorbic acid levels after partial hepatectomy.

    Maldonado, Mafalda / Inostroza, Eveling / Peña, Eduardo / Moncada, Natacha / Mardones, Lorena / Medina, José Luis / Muñoz, Alejandra / Gatica, Marcell / Villagrán, Marcelo / Escobar, Elizabeth / Mendoza, Pamela / Roa, Francisco J / González, Mauricio / Guzmán, Paula / Gutiérrez-Castro, Francisco A / Sweet, Karen / Muñoz-Montesino, Carola / Vera, Juan Carlos / Rivas, Coralia I

    Free radical biology & medicine

    2017  Volume 108, Page(s) 655–667

    Abstract: The liver has an extraordinary regenerative capacity in response to partial hepatectomy (PHx), which develops with neither tissue inflammation response nor alterations in the whole organism. This process is highly coordinated and it has been associated ... ...

    Abstract The liver has an extraordinary regenerative capacity in response to partial hepatectomy (PHx), which develops with neither tissue inflammation response nor alterations in the whole organism. This process is highly coordinated and it has been associated with changes in glutathione (GSH) metabolism. However, there are no reports indicating ascorbic acid (AA) levels after partial hepatectomy. AA and GSH act integrally as an antioxidant system that protects cells and tissues from oxidative damage and imbalance observed in a variety of diseases that affect the liver. Although rat hepatocytes are able to synthesize AA and GSH, which are the providers of AA for the whole organism, they also acquire AA from extracellular sources through the sodium-coupled ascorbic acid transporter-1 (SVCT1). Here, we show that hepatocytes from rat livers subjected to PHx increase their GSH and AA levels from 1 to 7 days post hepatectomy, whose peaks precede the peak in cell proliferation observed at 3 days post-hepatectomy. The increase in both antioxidants was associated with higher expression of the enzymes involved in their synthesis, such as the modifier subunit of enzyme glutamine cysteine ligase (GCLM), glutathione synthetase (GS), gulonolactonase (GLN) and gulonolactone oxidase (GULO). Importantly, rat hepatocytes, that normally exhibit kinetic evidence indicating only SVCT1-mediated transport of AA, lost more than 90% of their capacity to transport it at day 1 after PHx without evidence of recovery at day 7. This observation was in agreement with loss of SVCT1 protein expression, which was undetectable in hepatocytes as early as 2h after PHx, with partial recovery at day 7, when the regenerated liver weight returns to normal. We conclude that after PHx, rat hepatocytes enhance their antioxidant capacity by increasing GSH and AA levels prior to the proliferative peak. GSH and AA are increased by de novo synthesis, however paradoxically hepatocytes from rat subjected to PHx also suppress their capacity to acquire AA from extracellular sources through SVCT1.
    Language English
    Publishing date 2017-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 807032-5
    ISSN 1873-4596 ; 0891-5849
    ISSN (online) 1873-4596
    ISSN 0891-5849
    DOI 10.1016/j.freeradbiomed.2017.04.006
    Database MEDical Literature Analysis and Retrieval System OnLINE

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