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  1. Book: Site specific protein labeling

    Gautier, Arnaud

    methods and protocols

    (Methods in molecular biology ; 1266 ; Springer protocols)

    2015  

    Title variant Site-specific protein labeling
    Author's details Arnaud Gautier
    Series title Methods in molecular biology ; 1266
    Springer protocols
    Collection
    Language English
    Size XII, 267 S. : Ill., graph. Darst.
    Publisher Humana Press
    Publishing place New York u.a.
    Publishing country United States
    Document type Book
    HBZ-ID HT018526762
    ISBN 978-1-4939-2271-0 ; 1-4939-2271-8
    Database Catalogue ZB MED Medicine, Health

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  2. Article ; Online: Fluorescence-Activating and Absorption-Shifting Tags for Advanced Imaging and Biosensing.

    Gautier, Arnaud

    Accounts of chemical research

    2022  Volume 55, Issue 21, Page(s) 3125–3135

    Abstract: ConspectusFluorescent labels and biosensors play central roles in biological and medical research. Targeted to specific biomolecules or cells, they allow noninvasive imaging of the machinery that govern cells and organisms in real time. Recently, ... ...

    Abstract ConspectusFluorescent labels and biosensors play central roles in biological and medical research. Targeted to specific biomolecules or cells, they allow noninvasive imaging of the machinery that govern cells and organisms in real time. Recently, chemogenetic reporters made of organic dyes specifically anchored to genetic tags have challenged the paradigm of fully genetically encoded fluorescent proteins. Combining the advantage of synthetic fluorophores with the targeting selectivity of genetically encoded tags, these chemogenetic reporters open new exciting prospects for studying cell biochemistry and biology. In this Account, we present the growing toolbox of fluorescence-activating and absorption-shifting tags (FASTs), small monomeric proteins of 14 kDa (125 amino acids residues) that can be used as markers to monitor gene expression and protein localization in live cells and organisms. Engineered by directed protein evolution from the photoactive yellow protein (PYP) from the bacterium
    MeSH term(s) Biosensing Techniques/methods ; Fluorescent Dyes/chemistry ; Protein Engineering ; Proteins ; Rhodanine/metabolism
    Chemical Substances Fluorescent Dyes ; Proteins ; Rhodanine (7O50LKL2G8)
    Language English
    Publishing date 2022-10-21
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 1483291-4
    ISSN 1520-4898 ; 0001-4842
    ISSN (online) 1520-4898
    ISSN 0001-4842
    DOI 10.1021/acs.accounts.2c00098
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: A fluorescent reporter system for anaerobic thermophiles.

    Hocq, Rémi / Bottone, Sara / Gautier, Arnaud / Pflügl, Stefan

    Frontiers in bioengineering and biotechnology

    2023  Volume 11, Page(s) 1226889

    Abstract: Owing to their inherent capacity to make invisible biological processes visible and quantifiable, fluorescent reporter systems have numerous applications in biotechnology. For classical fluorescent protein systems (i.e., GFP and derivatives), chromophore ...

    Abstract Owing to their inherent capacity to make invisible biological processes visible and quantifiable, fluorescent reporter systems have numerous applications in biotechnology. For classical fluorescent protein systems (i.e., GFP and derivatives), chromophore maturation is O
    Language English
    Publishing date 2023-07-05
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2719493-0
    ISSN 2296-4185
    ISSN 2296-4185
    DOI 10.3389/fbioe.2023.1226889
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Vaccination acceptability in the French general population and related determinants, 2000-2021.

    Vaux, Sophie / Gautier, Arnaud / Nassany, Oriane / Bonmarin, Isabelle

    Vaccine

    2023  Volume 41, Issue 42, Page(s) 6281–6290

    Abstract: Background: This study describes the evolution of vaccination acceptability and associated determinants in the French general population between 2000 and 2021, and vaccinations with the highest vaccine hesitancy between 2010 and 2021.: Methods: Data ... ...

    Abstract Background: This study describes the evolution of vaccination acceptability and associated determinants in the French general population between 2000 and 2021, and vaccinations with the highest vaccine hesitancy between 2010 and 2021.
    Methods: Data were collected from the nine national 'Health Barometer' cross-sectional surveys conducted between 2000 and 2021. These surveys included French-speaking individuals aged 18-75 years old who were selected through randomly generated landline and mobile phone numbers. Participants were asked about their acceptability of vaccination in general and their vaccine hesitancy toward any particular vaccinations. Determinants of vaccination acceptability were studied using univariate and multivariate Poisson regressions.
    Results: The proportion of persons who found vaccination acceptable in general (i.e., answering "very" or "somewhat" favourable in the survey interview) decreased from 91.1% in 2000 to 61.2% in 2010 (the latter year coinciding with the 2009 H1N1 influenza pandemic), increased in 2014 (78.8%), slightly fluctuated until 2019 (74.2%), and increased again in both 2020 (80.0%) and 2021 (82.5%) during the COVID-19 pandemic. Irrespective of the year, acceptability was higher among persons with higher incomes, those with a higher education level, and individuals not living alone. In 2021, for the first time, vaccination acceptability was higher among persons over 44 years old (versus 18-24 year-olds) and among retired persons (versus workers). The highest hesitancy rate for a vaccine was for the 2009 H1N1 influenza virus in 2010 (41% answering "somewhat" or "very" unfavourable). In 2021, the highest rate was for the COVID-19 vaccine (21%).
    Discussion: Unlike the experience of the 2009 AH1N1 influenza pandemic, which led to a collapse in vaccination acceptability among the French general population, acceptability continued to increase during the COVID-19 pandemic. However, the pre-2010 level was not reached. Our results show a tendency towards a widening social and economic gap in terms of vaccine acceptability over time.
    Language English
    Publishing date 2023-09-05
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 605674-x
    ISSN 1873-2518 ; 0264-410X
    ISSN (online) 1873-2518
    ISSN 0264-410X
    DOI 10.1016/j.vaccine.2023.08.062
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Improving Split Reporters of Protein-Protein Interactions through Orthology-Based Protein Engineering.

    Rakotoarison, Louise-Marie / Tebo, Alison G / Böken, Dorothea / Board, Stephanie / El Hajji, Lina / Gautier, Arnaud

    ACS chemical biology

    2024  Volume 19, Issue 2, Page(s) 428–441

    Abstract: Protein-protein interactions (PPIs) can be detected through selective complementation of split fluorescent reporters made of two complementary fragments that reassemble into a functional fluorescent reporter when in close proximity. We previously ... ...

    Abstract Protein-protein interactions (PPIs) can be detected through selective complementation of split fluorescent reporters made of two complementary fragments that reassemble into a functional fluorescent reporter when in close proximity. We previously introduced splitFAST, a chemogenetic PPI reporter with rapid and reversible complementation. Here, we present the engineering of splitFAST2, an improved reporter displaying higher brightness, lower self-complementation, and higher dynamic range for optimal monitoring of PPI using an original protein engineering strategy that exploits proteins with orthology relationships. Our study allowed the identification of a system with improved properties and enabled a better understanding of the molecular features controlling the complementation properties. Because of the rapidity and reversibility of its complementation, its low self-complementation, high dynamic range, and improved brightness, splitFAST2 is well suited to study PPI with high spatial and temporal resolution, opening great prospects to decipher the role of PPI in various biological contexts.
    MeSH term(s) Protein Interaction Mapping ; Proteins/genetics ; Proteins/metabolism ; Protein Engineering
    Chemical Substances Proteins
    Language English
    Publishing date 2024-01-30
    Publishing country United States
    Document type Journal Article
    ISSN 1554-8937
    ISSN (online) 1554-8937
    DOI 10.1021/acschembio.3c00631
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Engineering of Tunable Allosteric-like Fluorogenic Protein Sensors.

    Broch, Fanny / El Hajji, Lina / Pietrancosta, Nicolas / Gautier, Arnaud

    ACS sensors

    2023  Volume 8, Issue 10, Page(s) 3933–3942

    Abstract: Optical protein sensors that enable detection of relevant biomolecules of interest play central roles in biological research. Coupling fluorescent reporters with protein sensing units has enabled the development of a wide range of biosensors that ... ...

    Abstract Optical protein sensors that enable detection of relevant biomolecules of interest play central roles in biological research. Coupling fluorescent reporters with protein sensing units has enabled the development of a wide range of biosensors that recognize analytes with high selectivity. In these sensors, analyte recognition induces a conformational change in the protein sensing unit that can modulate the optical signal of the fluorescent reporter. Here, we explore various designs for the creation of tunable allosteric-like fluorogenic protein sensors through incorporation of sensing protein units within the chemogenetic fluorescence-activating and absorption-shifting tag (FAST) that selectively binds and stabilizes the fluorescent state of 4-hydroxybenzylidene rhodanine (HBR) analogs. Conformational coupling allowed us to design analyte-responsive optical protein sensors through allosteric-like modulation of fluorogen binding.
    MeSH term(s) Proteins ; Fluorescence ; Fluorescent Dyes/chemistry
    Chemical Substances Proteins ; Fluorescent Dyes
    Language English
    Publishing date 2023-10-13
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2379-3694
    ISSN (online) 2379-3694
    DOI 10.1021/acssensors.3c01536
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Cage-like structures based on constrained cyclic arylopeptoids.

    Akhdar, Ayman / Nauton, Lionel / Jouffret, Laurent / Faure, Sophie / Gautier, Arnaud

    Chemical communications (Cambridge, England)

    2023  Volume 59, Issue 52, Page(s) 8087–8090

    Abstract: The access to cupola-like or tube-like structures ... ...

    Abstract The access to cupola-like or tube-like structures from
    Language English
    Publishing date 2023-06-27
    Publishing country England
    Document type Journal Article
    ZDB-ID 1472881-3
    ISSN 1364-548X ; 1359-7345 ; 0009-241X
    ISSN (online) 1364-548X
    ISSN 1359-7345 ; 0009-241X
    DOI 10.1039/d3cc01956j
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: SARS-CoV-2 testing, infection and places of contamination in France, a national cross-sectional study, December 2021.

    Vaux, Sophie / Gautier, Arnaud / Soullier, Noemie / Levy-Bruhl, Daniel

    BMC infectious diseases

    2023  Volume 23, Issue 1, Page(s) 279

    Abstract: Background: This study aimed to describe the use of diagnostic testing for SARS-CoV-2 in France until December 2021, the characteristics of people infected, and places of contamination.: Methods: Data were collected from the national 2021 Health ... ...

    Abstract Background: This study aimed to describe the use of diagnostic testing for SARS-CoV-2 in France until December 2021, the characteristics of people infected, and places of contamination.
    Methods: Data were collected from the national 2021 Health Barometer cross-sectional study, which was conducted between February and December 2021 and included French-speaking individuals aged 18-85 years old selected through randomly generated landline and mobile phone numbers. Participants were interviewed about COVID-19-like symptoms in the previous 12 months, diagnostic testing for SARS-CoV-2, positive diagnosis for SARS-CoV-2, and the place(s) of contamination. Determinants of diagnostic testing and of infection were studied using univariate and multivariate Poisson regressions.
    Results: A total of 24,514 persons participated in the study. We estimated that 66.4% [65.0-67.7] of persons had been tested for SARS-CoV-2 the last time they experienced COVID-19-like symptoms, and that 9.8% [9.3-10.3] of the population in France - with or without symptoms - had been tested positive. Diagnostic testing was less frequent in men, unemployed persons, and people living alone; it was also less frequent during the first months of the pandemic. The estimated proportion of the population infected was higher in healthcare professionals (PRa: 1.5 [1.3-1.7]), those living in large cities ( > = 200 000 inhabitants, and Paris area) (1.4 [1.2-1.6]), and in households comprising > 3 persons (1.7 [1.5-2.0]). It was lower in retired persons (0.8 [0.6-0.97]) and those over 65 years old (0.6 [0.4-0.9]). Almost two-thirds (65.7%) of infected persons declared they knew where they were contaminated; 5.8% [4.5-7.4] reported being contaminated outdoors, 47.9% [44.8-51.0] in unventilated indoor environments, and 43.4% [40.3-46.6] in ventilated indoor environments. Specifically, 51.1% [48.0-54.2] declared they were contaminated at home or in a family of friend's house, 29.1% [26.4-31.9] at their workplace, 13.9% [11.9-16.1] in a healthcare structure, and 9.0% [7.4-10.8] in a public eating place (e.g., cafeteria, bar, restaurant).
    Conclusions: To limit viral spread, preventive actions should preferentially target persons tested least frequently and those at a higher risk of infection. They should also target contamination in households, healthcare structures, and public eating places. Importantly, contamination is most frequent in places where prevention measures are most difficult to implement.
    MeSH term(s) Male ; Humans ; Adolescent ; Young Adult ; Adult ; Middle Aged ; Aged ; Aged, 80 and over ; SARS-CoV-2 ; COVID-19/diagnosis ; COVID-19/epidemiology ; Cross-Sectional Studies ; COVID-19 Testing ; France/epidemiology
    Language English
    Publishing date 2023-05-03
    Publishing country England
    Document type Journal Article
    ZDB-ID 2041550-3
    ISSN 1471-2334 ; 1471-2334
    ISSN (online) 1471-2334
    ISSN 1471-2334
    DOI 10.1186/s12879-023-08257-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Arylopeptoid oligomers functionalised by combinatorial or sequential on-resin click chemistry using a copper(I)-N-heterocyclic carbene catalyst.

    Akhdar, Ayman / Faure, Sophie / Gautier, Arnaud

    Organic & biomolecular chemistry

    2022  Volume 20, Issue 12, Page(s) 2402–2406

    Abstract: An efficient on-resin click chemistry protocol using a stable copper(I)-N-heterocyclic carbene catalyst is developed for post-functionalization ... ...

    Abstract An efficient on-resin click chemistry protocol using a stable copper(I)-N-heterocyclic carbene catalyst is developed for post-functionalization of
    MeSH term(s) Catalysis ; Click Chemistry ; Copper ; Methane/analogs & derivatives
    Chemical Substances carbene (2465-56-7) ; Copper (789U1901C5) ; Methane (OP0UW79H66)
    Language English
    Publishing date 2022-03-23
    Publishing country England
    Document type Journal Article
    ZDB-ID 2097583-1
    ISSN 1477-0539 ; 1477-0520
    ISSN (online) 1477-0539
    ISSN 1477-0520
    DOI 10.1039/d2ob00163b
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Isolating and Engineering Fluorescence-Activating Proteins Using Yeast Surface Display.

    El Hajji, Lina / Benaissa, Hela / Gautier, Arnaud

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2491, Page(s) 593–626

    Abstract: This protocol describes the workflow to isolate and engineer fluorescence-activating proteins by yeast surface display. Fluorescence-activating proteins are an emerging class of fluorescent chemogenetic reporters for monitoring gene expression and ... ...

    Abstract This protocol describes the workflow to isolate and engineer fluorescence-activating proteins by yeast surface display. Fluorescence-activating proteins are an emerging class of fluorescent chemogenetic reporters for monitoring gene expression and protein localization in living cells and organisms. They become fluorescent upon binding exogenously applied fluorogenic organic dyes. Efficient fluorescence-activating proteins can be selected from yeast-displayed libraries by iterative rounds of fluorescence-activated cell sorting. The overall strategy is described, as well as a strategy for characterizing the affinity and spectroscopic properties of the selected clones.
    MeSH term(s) Flow Cytometry/methods ; Protein Engineering/methods ; Proteins/metabolism ; Saccharomyces cerevisiae/metabolism
    Chemical Substances Proteins
    Language English
    Publishing date 2022-04-28
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2285-8_25
    Database MEDical Literature Analysis and Retrieval System OnLINE

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