LIVIVO - Das Suchportal für Lebenswissenschaften

switch to English language
Zurück zur letzten Suche Suchhistorie
Erweiterte Suche

Ihre letzten Suchen

  1. AU="Gawade, Sanjay"
  2. AU=Vaghardoost Reza
  3. AU="Schultz, Tracey L"
  4. AU=Stefanoska Kristie
  5. AU=Ray Debraj
  6. AU="Strahler, E A"
  7. AU="Lee, Wang Jun"
  8. AU="Fiona McDuie"
  9. AU="You, Kisung"
  10. AU="Sanjuan, Miguel A" AU="Sanjuan, Miguel A"
  11. AU=Weingarten-Gabbay Shira
  12. AU="Choukr-Allah, Redouane"
  13. AU="Mills, Mary Katherine"
  14. AU="Vajente, G."
  15. AU="Bhatnagar, R.C"
  16. AU="Prasad Sarkale"
  17. AU="Manfredonia, Laura"
  18. AU="Linssen, L"
  19. AU="Davide, Borroni"
  20. AU="Ingrid M. Wentzensen"
  21. AU="A.Parida, "
  22. AU="Zhu, D H"
  23. AU=Ulloa Luis
  24. AU="Böhme, Elisa"
  25. AU=Trko?lu Oya
  26. AU="Levine, Zoe"
  27. AU="Banaszkiewicz, Paul A."
  28. AU="Datrier, Laurence E. H."
  29. AU=Fala Loretta
  30. AU="McGuckin, M M"
  31. AU="Winlaw, David S"
  32. AU="Gökmen, M Refik"
  33. AU="Islam, Tousif"
  34. AU="Szczepanczyk, Marek J"
  35. AU="Boregowda, Siddaraju"
  36. AU="Lomidzew, D."

Suchergebnis

Treffer 1 - 3 von insgesamt 3

Suchoptionen

  1. Artikel ; Online: Ex vivo model for elucidating the functional and structural differentiation of the embryonic mouse thyroid.

    Carré, Aurore / Gawade, Sanjay / Dertschnig, Simone / Hafen, Kathrin / Polak, Michel / Szinnai, Gabor

    Molecular and cellular endocrinology

    2020  Band 510, Seite(n) 110834

    Abstract: Terminal thyroid gland differentiation - the last developmental step needed to enable thyroid hormone (T4) synthesis - involves profound structural and biochemical changes in the thyroid follicular cells (TFCs). We aimed to develop an ex vivo thyroid ... ...

    Abstract Terminal thyroid gland differentiation - the last developmental step needed to enable thyroid hormone (T4) synthesis - involves profound structural and biochemical changes in the thyroid follicular cells (TFCs). We aimed to develop an ex vivo thyroid model of embryonic mouse thyroid that would replicate the in vivo TFC differentiation program. E13.5 thyroid explants were cultured ex vivo in chemically defined medium for 7 days. Immunostaining and qPCR of thyroid explants showed thyroglobulin production onset, follicle formation, and T4 synthesis onset in 1-, 3-, and 5-day-old cultures, respectively. Differentiation was maintained and follicular growth continued throughout the 7-day culture period. Pharmacological approaches to culture inhibition were performed successfully in the ex vivo thyroids. Our robust and well described ex vivo thyroid culture model replicates the sequence of thyroid differentiation to T4 synthesis seen in vivo. This model can be used to test the effects of pharmacological inhibitors on thyroid hormone production.
    Mesh-Begriff(e) Animals ; Cell Differentiation ; Embryo, Mammalian/anatomy & histology ; Embryonic Development/genetics ; Gene Expression Regulation, Developmental ; Mice ; Microdissection ; Models, Biological ; Receptors, Notch/metabolism ; Thyroglobulin/metabolism ; Thyroid Gland/anatomy & histology ; Thyroid Gland/embryology ; Thyroid Gland/physiology ; Tissue Survival/physiology
    Chemische Substanzen Receptors, Notch ; Thyroglobulin (9010-34-8)
    Sprache Englisch
    Erscheinungsdatum 2020-04-28
    Erscheinungsland Ireland
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 187438-x
    ISSN 1872-8057 ; 0303-7207
    ISSN (online) 1872-8057
    ISSN 0303-7207
    DOI 10.1016/j.mce.2020.110834
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 1127: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  2. Artikel ; Online: Cell Growth Dynamics in Embryonic and Adult Mouse Thyroid Revealed by a Novel Approach to Detect Thyroid Gland Subpopulations.

    Gawade, Sanjay / Mayer, Carlos / Hafen, Katrin / Barthlott, Thomas / Krenger, Werner / Szinnai, Gabor

    Thyroid : official journal of the American Thyroid Association

    2016  Band 26, Heft 4, Seite(n) 591–599

    Abstract: Background: The thyroid is composed of endocrine epithelial cells, blood vessels, and mesenchyme. However, no data exist thus far on absolute cell numbers, relative distribution, and proliferation of the different cell populations in the developing and ... ...

    Abstract Background: The thyroid is composed of endocrine epithelial cells, blood vessels, and mesenchyme. However, no data exist thus far on absolute cell numbers, relative distribution, and proliferation of the different cell populations in the developing and mature thyroid. The aim of this study was therefore to establish a flow cytometry protocol that allows detection and quantification of discrete cell populations in embryonic and adult murine thyroid tissues.
    Methods: Cell-type anti-mouse specific antibodies were used for erythroid cells (Ter119), hematopoietic cells (CD45), epithelial cells (EpCam/CD326, E-cadherin/CD324), thyroid follicular cells and C-cells (Nkx2-1), endothelial cells (Pecam/CD31, Icam-1/CD54), and fibroblasts (PDGFRa/CD140a). Proliferating cells were detected after labeling with 5-bromo-2'-deoxyuridine (BrdU). For flow cytometry analyses, micro-dissected embryonic (E) and adult thyroids were pooled (E13.5, n = 25; E15.5, n = 15; E17.5, n = 15; adult, n = 4) in one sample.
    Results: The absolute parenchymal cell numbers per mouse thyroid (M ± SD), excluding the large number of CD45(+) and Ter119(+) cells, increased from 7425 ± 1338 at E13.5 to 271,561 ± 22,325 in adult tissues. As expected, Nkx2-1(+) cells represented the largest cell population in adult tissues (61.2 ± 1.1%). Surprisingly, at all three embryonic stages analyzed, thyroid follicular cells and C-cells accounted only for a small percentage of the total thyroid cell mass (between 4.7 ± 0.4% and 9.4 ± 1.6%). In contrast, the largest cell population at all three embryonic stages was identified as PDGFRa/CD140a(+) fibroblasts (61.4 ± 0.4% to 77.3 ± 1.1%). However, these cells represented the smallest population in adult tissues (5.2 ± 0.8%). Pecam/CD31(+) endothelial cells increased from E13.5 to E15.5 from 3.7 ± 0.8% to 8.5 ± 3.0%, then remained stable at E17.5 and adult tissues. Proliferation rates were sizable during the entire organogenesis but differed between cell populations, with distinct proliferative peaks at E13.5 in epithelial cells (32.7 ± 0.6% BrdU(+) cells), and at E15.5 in endothelial cells (22.4 ± 2.4% BrdU(+) cells). Fibroblasts showed a constant proliferation rate in embryonic tissues. In adult tissues, BrdU(+) cells were between 0.1% and 0.4% in all cell types.
    Conclusions: Using a novel flow cytometry-based method, a previously unobserved highly dynamic growth pattern of thyroid cell populations during embryogenesis was uncovered. This approach will provide a useful new tool for cell function analyses in murine thyroid disease models.
    Mesh-Begriff(e) Animals ; Antibodies/chemistry ; Cell Differentiation ; Cell Proliferation ; Cell Separation ; Epithelial Cells/cytology ; Female ; Fibroblasts/cytology ; Flow Cytometry ; Mesoderm/cytology ; Mice ; Thyroid Gland/cytology ; Thyroid Gland/embryology
    Chemische Substanzen Antibodies
    Sprache Englisch
    Erscheinungsdatum 2016-04
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1086044-7
    ISSN 1557-9077 ; 1050-7256
    ISSN (online) 1557-9077
    ISSN 1050-7256
    DOI 10.1089/thy.2015.0523
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 3307: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MO 106: Hefte anzeigen

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  3. Artikel ; Online: TUBB1 mutations cause thyroid dysgenesis associated with abnormal platelet physiology.

    Stoupa, Athanasia / Adam, Frédéric / Kariyawasam, Dulanjalee / Strassel, Catherine / Gawade, Sanjay / Szinnai, Gabor / Kauskot, Alexandre / Lasne, Dominique / Janke, Carsten / Natarajan, Kathiresan / Schmitt, Alain / Bole-Feysot, Christine / Nitschke, Patrick / Léger, Juliane / Jabot-Hanin, Fabienne / Tores, Frédéric / Michel, Anita / Munnich, Arnold / Besmond, Claude /
    Scharfmann, Raphaël / Lanza, François / Borgel, Delphine / Polak, Michel / Carré, Aurore

    EMBO molecular medicine

    2019  Band 10, Heft 12

    Abstract: The genetic causes of congenital hypothyroidism due to thyroid dysgenesis (TD) remain largely unknown. We identified three ... ...

    Abstract The genetic causes of congenital hypothyroidism due to thyroid dysgenesis (TD) remain largely unknown. We identified three novel
    Mesh-Begriff(e) Animals ; Blood Platelets/cytology ; Blood Platelets/pathology ; Humans ; Mice ; Mice, Knockout ; Mutation ; Platelet Aggregation ; Thyroid Dysgenesis/genetics ; Thyroid Dysgenesis/pathology ; Tubulin/genetics
    Chemische Substanzen TUBB1 protein, human ; Tubulin
    Sprache Englisch
    Erscheinungsdatum 2019-01-23
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2467145-9
    ISSN 1757-4684 ; 1757-4676
    ISSN (online) 1757-4684
    ISSN 1757-4676
    DOI 10.15252/emmm.201809569
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 6784: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

Zum Seitenanfang