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  1. Article ; Online: CELL-SELEX

    Hans P. Wendel / Angela Paul / Gerhard Ziemer / Ke-Tai Guo

    International Journal of Molecular Sciences, Vol 9, Iss 4, Pp 668-

    Novel Perspectives of Aptamer-Based Therapeutics

    2008  Volume 678

    Abstract: Aptamers, single stranded DNA or RNA molecules, generated by a method called SELEX (systematic evolution of ligands by exponential enrichment) have been widely used in various biomedical applications. The newly developed Cell-SELEX (cell based-SELEX) ... ...

    Abstract Aptamers, single stranded DNA or RNA molecules, generated by a method called SELEX (systematic evolution of ligands by exponential enrichment) have been widely used in various biomedical applications. The newly developed Cell-SELEX (cell based-SELEX) targeting whole living cells has raised great expectations for cancer biology, -therapy and regenerative medicine. Combining nanobiotechnology with aptamers, this technology opens the way to more sophisticated applications in molecular diagnosis. This paper gives a review of recent developments in SELEX technologies and new applications of aptamers.
    Keywords cell-SELEX ; aptamer ; nanotechnology ; Chemistry ; QD1-999 ; Science ; Q ; DOAJ:Chemistry (General) ; DOAJ:Chemistry
    Language English
    Publishing date 2008-04-01T00:00:00Z
    Publisher Molecular Diversity Preservation International
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Upgrading SELEX Technology by Using Lambda Exonuclease Digestion for Single-Stranded DNA Generation

    Meltem Avci-Adali / Angel Paul / Nadj Wilhelm / Gerhard Ziemer / Hans Peter Wendel

    Molecules, Vol 15, Iss 1, Pp 1-

    2009  Volume 11

    Abstract: The generation of single-stranded DNA (ssDNA) molecules plays a key role in the SELEX (Systematic Evolution of Ligands by EXponential enrichment) combinatorial chemistry process and numerous molecular biology techniques and applications, such as DNA ... ...

    Abstract The generation of single-stranded DNA (ssDNA) molecules plays a key role in the SELEX (Systematic Evolution of Ligands by EXponential enrichment) combinatorial chemistry process and numerous molecular biology techniques and applications, such as DNA sequencing, single-nucleotide polymorphism (SNP) analysis, DNA chips, DNA single-strand conformation polymorphism (SSCP) analysis and many other techniques. The purity and yield of ssDNA can affect the success of each application. This study compares the two ssDNA production methods, the strand separation by streptavidin-coated magnetic beads and alkaline denaturation and the lambda exonuclease digestion, in regard to the purity of generated ssDNA and the efficiency. Here, we demonstrate the considerable benefits of ssDNA production by lambda exonuclease digestion for in vitro selection of DNA aptamers. We believe that the generation of ssDNA aptamers using this method will greatly improve the success rate of SELEX experiments concerning the recovery of target-specific aptamers.
    Keywords aptamers ; strand separation ; streptavidin-coated beads ; lambda exonuclease ; single-stranded DNA ; Organic chemistry ; QD241-441
    Subject code 612
    Language English
    Publishing date 2009-12-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: AN INVITATION TO THE MEDICAL STUDENTS OF THE WORLD TO JOIN THE GLOBAL COALITION TO IMPROVE CARE FOR CHILDREN AND ADULTS WITH CONGENITAL HEART DISEASE ACROSS THE WORLD

    Pierre-Luc Bernier / Noritaka Ota / Christo I. Tchervenkov / Jeffrey P. Jacobs / Giovanni Stellin / Hiromi Kurosawa / Constantine Mavroudis / Sertac Cicek / Zohair Al-Halees / Martin Elliott / Marcelo Jatene / Richard A. Jonas / Rob Kinsley / Christian Kreutzer / Juan Leon-Wyss / Jinfen Liu / Bohdan Maruszewski / Graham Nunn / Samuel Ramirez-Marroquin /
    Nestor Sandaval / Shunji Sano / George Sarris / Rajesh Sharma / Thomas Spray / Ross Ungerleider / Hervé Yangni-Angate / Gerhard Ziemer

    McGill Journal of Medicine, Vol 11, Iss

    2020  Volume 2

    Abstract: ... N/ ... ...

    Abstract N/A
    Keywords congenital heart disease ; pediatric ; Medicine ; R
    Language English
    Publishing date 2020-12-01T00:00:00Z
    Publisher McGill University
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: NCO-sP(EO-stat-PO) Coatings on Gold Sensors—a QCM Study of Hemocompatibility

    Frank K. Gehring / Hans P. Wendel / Frank Rupp / Jürgen Geis-Gerstorfer / Hinnak Northoff / Gerhard Ziemer / Jürgen Groll / Lothar Müller / Daniel Bünger / Mirjam Eichler / Stefan Sinn

    Sensors, Vol 11, Iss 5, Pp 5253-

    2011  Volume 5269

    Abstract: The reliability of implantable blood sensors is often hampered by unspecific adsorption of plasma proteins and blood cells. This not only leads to a loss of sensor signal over time, but can also result in undesired host vs. graft reactions. Within this ... ...

    Abstract The reliability of implantable blood sensors is often hampered by unspecific adsorption of plasma proteins and blood cells. This not only leads to a loss of sensor signal over time, but can also result in undesired host vs. graft reactions. Within this study we evaluated the hemocompatibility of isocyanate conjugated star shaped polytheylene oxide—polypropylene oxide co-polymers NCO-sP(EO-stat-PO) when applied to gold surfaces as an auspicious coating material for gold sputtered blood contacting sensors. Quartz crystal microbalance (QCM) sensors were coated with ultrathin NCO-sP(EO-stat-PO) films and compared with uncoated gold sensors. Protein resistance was assessed by QCM measurements with fibrinogen solution and platelet poor plasma (PPP), followed by quantification of fibrinogen adsorption. Hemocompatibility was tested by incubation with human platelet rich plasma (PRP). Thrombin antithrombin-III complex (TAT), β-thromboglobulin (β-TG) and platelet factor 4 (PF4) were used as coagulation activation markers. Furthermore, scanning electron microscopy (SEM) was used to visualize platelet adhesion to the sensor surfaces. Compared to uncoated gold sensors, NCO-sP(EO-stat-PO) coated sensors revealed significant better resistance against protein adsorption, lower TAT generation and a lower amount of adherent platelets. Moreover, coating with ultrathin NCO-sP(EO-stat-PO) films creates a cell resistant hemocompatible surface on gold that increases the chance of prolonged sensor functionality and can easily be modified with specific receptor molecules.
    Keywords surface coating ; biosensors ; hemocompatibility ; QCM ; protein adsorption ; Technology (General) ; T1-995 ; Technology ; T ; DOAJ:Technology (General) ; DOAJ:Technology and Engineering ; Analytical chemistry ; QD71-142 ; Chemistry ; QD1-999 ; Science ; Q ; DOAJ:Analytical Chemistry ; DOAJ:Chemistry
    Language English
    Publishing date 2011-05-01T00:00:00Z
    Publisher Molecular Diversity Preservation International
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Inter-library loan at ZB MED

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