Article ; Online: How Low Can You Go? Low Densities of Poly(ethylene glycol) Surfactants Attract Stealth Proteins.
2018 Volume 18, Issue 9, Page(s) e1800075
Abstract: It is now well-established that the surface chemistry and "stealth" surface functionalities such as poly(ethylene glycol) (PEG) chains of nanocarriers play an important role to decrease unspecific protein adsorption of opsonizing proteins, to increase ... ...
Abstract | It is now well-established that the surface chemistry and "stealth" surface functionalities such as poly(ethylene glycol) (PEG) chains of nanocarriers play an important role to decrease unspecific protein adsorption of opsonizing proteins, to increase the enrichment of specific stealth proteins, and to prolong the circulation times of the nanocarriers. At the same time, PEG chains are used to provide colloidal stability for the nanoparticles. However, it is not clear how the chain length and density influence the unspecific and specific protein adsorption keeping at the same time the stability of the nanoparticles in a biological environment. Therefore, this study aims at characterizing the protein adsorption patterns depending on PEG chain length and density to define limits for the amount of PEG needed for a stealth effect by selective protein adsorption as well as colloidal stability during cell experiments. PEG chains are introduced using the PEGylated Lutensol AT surfactants, which allow easy modification of the nanoparticle surface. These findings indicate that a specific enrichment of stealth proteins already occurs at low PEG concentrations; for the decrease of unspecific protein adsorption and finally the colloidal stability a full surface coverage is advised. |
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MeSH term(s) | Adsorption ; Animals ; Clusterin/chemistry ; Clusterin/metabolism ; Colloids/chemistry ; Humans ; Magnetic Resonance Spectroscopy ; Mice ; Nanoparticles/chemistry ; Plasma/chemistry ; Polyethylene Glycols/chemistry ; Polyethylene Glycols/metabolism ; Polystyrenes/chemistry ; Protein Corona/chemistry ; RAW 264.7 Cells ; Sodium Dodecyl Sulfate/chemistry ; Surface-Active Agents/chemistry ; Surface-Active Agents/metabolism |
Chemical Substances | Clusterin ; Colloids ; Polystyrenes ; Protein Corona ; Surface-Active Agents ; Sodium Dodecyl Sulfate (368GB5141J) ; Polyethylene Glycols (3WJQ0SDW1A) |
Language | English |
Publishing date | 2018-06-25 |
Publishing country | Germany |
Document type | Journal Article ; Research Support, Non-U.S. Gov't |
ZDB-ID | 2039130-4 |
ISSN | 1616-5195 ; 1616-5187 |
ISSN (online) | 1616-5195 |
ISSN | 1616-5187 |
DOI | 10.1002/mabi.201800075 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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