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  1. Article ; Online: The Role of MexCD-OprJ and MexEF-OprN Efflux Systems in the Multiple Antibiotic Resistance of Pseudomonas aeruginosa Isolated from Clinical Samples.

    Jamal, Zeynab / Gholami, Mehrdad / Ebrahimzadeh, Mohammad Ali / Goli, Hamid Reza

    Current microbiology

    2023  Volume 80, Issue 7, Page(s) 221

    Abstract: Increasing antimicrobial resistance and the development of multi-drug resistant (MDR) Pseudomonas aeruginosa is dependent on the expression of efflux pumps. This study aimed to investigate the role of overexpression of MexCD-OprJ and MexEF-OprN efflux ... ...

    Abstract Increasing antimicrobial resistance and the development of multi-drug resistant (MDR) Pseudomonas aeruginosa is dependent on the expression of efflux pumps. This study aimed to investigate the role of overexpression of MexCD-OprJ and MexEF-OprN efflux pumps in reduced susceptibility to antimicrobial agents among P. aeruginosa strains. Totally, 100 clinical isolates of P. aeruginosa were collected from patients and the strains were identified by standard diagnostic tests. The MDR isolates were detected using the disk agar diffusion method. The expression levels of MexCD-OprJ and MexEF-OprN efflux pumps were evaluated by the real-time PCR. Forty-one isolates showed MDR phenotype, while piperacillin-tazobactam and levofloxacin were the most- and least-effective antibiotics, respectively. Also, all 41 MDR isolates showed a more than tenfold increase in the expression of mexD and mexF genes. In this study, a significant relationship was observed between the rate of antibiotic resistance, the emergence of MDR strains, and increasing the expression levels of MexEF-OprN and MexCD-OprJ efflux pumps (P < 0.05). Efflux systems mediated resistance was a noteworthy mechanism causative to multidrug resistance in P. aeruginosa clinical isolates. The study results demonstrated mexE and mexF overexpression as the primary mechanism conferring in the emergence of MDR phenotypes among P. aeruginosa strains. In addition, we also show that piperacillin/tazobactam exhibited a stronger ability in the management of infections caused by MDR P. aeruginosa in this area.
    MeSH term(s) Membrane Transport Proteins/genetics ; Membrane Transport Proteins/metabolism ; Pseudomonas aeruginosa/genetics ; Bacterial Outer Membrane Proteins/genetics ; Bacterial Outer Membrane Proteins/metabolism ; Drug Resistance, Microbial ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/metabolism ; Piperacillin/pharmacology ; Piperacillin/metabolism ; Tazobactam/metabolism ; Tazobactam/pharmacology ; Microbial Sensitivity Tests
    Chemical Substances Membrane Transport Proteins ; Bacterial Outer Membrane Proteins ; Anti-Bacterial Agents ; Piperacillin (X00B0D5O0E) ; Tazobactam (SE10G96M8W)
    Language English
    Publishing date 2023-05-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 134238-1
    ISSN 1432-0991 ; 0343-8651
    ISSN (online) 1432-0991
    ISSN 0343-8651
    DOI 10.1007/s00284-023-03330-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: A Survey on Exposure Parameters Variation due to Aging in Radiology Devices.

    Nemati, Fataneh / Mohammadi, Mahdi / Gholami, Mehrdad

    Journal of biomedical physics & engineering

    2021  Volume 11, Issue 3, Page(s) 407–412

    Abstract: The inevitable use of medical imaging examinations and lack of a suitable alternative lead to the need to control and minimize the amount of radiation from such artificial medical sources. To assess the relation between exposure parameters and lifetime ... ...

    Abstract The inevitable use of medical imaging examinations and lack of a suitable alternative lead to the need to control and minimize the amount of radiation from such artificial medical sources. To assess the relation between exposure parameters and lifetime of radiology devices, quality control tests were carried out on 13 radiology devices in 11 general hospitals. In this study, a barracuda dosimeter, SE-43137 Sweden, was calibrated to measure and record the quantities of kVp, mAs and exposure parameters. In all the devices using applying the minimum and maximum values of kVp, the minimum and maximum values of the internal resistances were calculated. The lowest mR/mA for the device C was observed at a flow rate of 200 mA (equal to 2,425), while the highest value was for the device A (2) at a current intensity of 200 mA (equal to 14.625). By increasing the age of the device, the output of the device is reduced. Therefore, to compensate for this decrease in the output, higher exposure conditions are usually applied to the device, which can greatly increase the damage to the device.
    Language English
    Publishing date 2021-06-01
    Publishing country Iran
    Document type Journal Article
    ZDB-ID 2673599-4
    ISSN 2251-7200
    ISSN 2251-7200
    DOI 10.31661/jbpe.v0i0.1154
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: An overview and bibliometric analysis on the colorectal cancer therapy by magnetic functionalized nanoparticles for the responsive and targeted drug delivery.

    Darroudi, Mahdieh / Gholami, Mehrdad / Rezayi, Majid / Khazaei, Majid

    Journal of nanobiotechnology

    2021  Volume 19, Issue 1, Page(s) 399

    Abstract: With the growing demands for personalized medicine and medical devices, nanomedicine is a modern scientific field, and research continues to apply nanomaterials for therapeutic and damaged tissue diagnosis. In this regard, substantial progress has been ... ...

    Abstract With the growing demands for personalized medicine and medical devices, nanomedicine is a modern scientific field, and research continues to apply nanomaterials for therapeutic and damaged tissue diagnosis. In this regard, substantial progress has been made in synthesizing magnetic nanoparticles with desired sizes, chemical composition, morphologies, and surface chemistry. Among these materials, nanomagnetic iron oxides have demonstrated promise as unique drug delivery carriers due to cancer treatment. This carrier could lead to responsive properties to a specific trigger, including heat, pH, alternative magnetic field, or even enzymes, through functionalization and coating of magnetic nanoparticles, along with biocompatibility, good chemical stability, easy functionalization, simple processing, and ability to localize to the tumor site with the assistance of external magnetic field. Current studies have focused on magnetic nanoparticles' utilities in cancer therapy, especially for colorectal cancer. Additionally, a bibliometric investigation was performed on the public trends in the field of the magnetic nanoparticle to drug delivery and anticancer, which represented progressing applications of these carriers in the multidisciplinary zones with a general view on future research and identified potential opportunities and challenges. Furthermore, we outline the current challenges and forthcoming research perspective for high performance and fostering advanced MNPs in colorectal cancer treatment.
    MeSH term(s) Animals ; Antineoplastic Agents/chemistry ; Antineoplastic Agents/therapeutic use ; Bibliometrics ; Colorectal Neoplasms/diagnosis ; Colorectal Neoplasms/therapy ; Drug Delivery Systems/methods ; Humans ; Magnetite Nanoparticles/chemistry ; Magnetite Nanoparticles/therapeutic use ; Mice ; Nanomedicine ; Precision Medicine
    Chemical Substances Antineoplastic Agents ; Magnetite Nanoparticles
    Language English
    Publishing date 2021-11-29
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2100022-0
    ISSN 1477-3155 ; 1477-3155
    ISSN (online) 1477-3155
    ISSN 1477-3155
    DOI 10.1186/s12951-021-01150-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Characterization of Antimicrobial Activities

    Roozbahani, Fatemeh / Ahanjan, Mohammad / Moshiri, Mona / Abediankenari, Saeid / Goli, Hamid Reza / Kakavan, Maedeh / Gholami, Mehrdad

    Foodborne pathogens and disease

    2024  

    Abstract: Bifidobacterium ... ...

    Abstract Bifidobacterium animalis
    Language English
    Publishing date 2024-04-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2148479-X
    ISSN 1556-7125 ; 1535-3141
    ISSN (online) 1556-7125
    ISSN 1535-3141
    DOI 10.1089/fpd.2023.0182
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Antimicrobial resistance patterns, virulence genes, and biofilm formation in enterococci strains collected from different sources.

    Ghazvinian, Maryam / Asgharzadeh Marghmalek, Saba / Gholami, Mehrdad / Amir Gholami, Sanaz / Amiri, Elham / Goli, Hamid Reza

    BMC infectious diseases

    2024  Volume 24, Issue 1, Page(s) 274

    Abstract: Background: Currently, antibiotic-resistant strains of Enterococcus are considered to be one of the critical health challenges globally. This study aimed to investigate the antibiotic susceptibility pattern, biofilm formation capacity, and virulence ... ...

    Abstract Background: Currently, antibiotic-resistant strains of Enterococcus are considered to be one of the critical health challenges globally. This study aimed to investigate the antibiotic susceptibility pattern, biofilm formation capacity, and virulence genes of enterococci isolated from different sources.
    Methods: In this cross-sectional study, environmental and fecal samples were collected from the hospital environment, volunteers, and hospital staff from October 2018 to August 2019. The isolates were identified by morphological and biochemical tests (gram staining, catalase, bile resistance, esculin hydrolysis, carbohydrate fermentation, growth in 6.5% NaCl, Pyrrolidonyl arylamidase, arginine dehydrolase), and PCR for ddl gene. An antimicrobial susceptibility test was performed by the standard disk agar diffusion method according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. Quantitative microplate assays were used to assess biofilm production. The bacterial DNAs were extracted by alkaline lysis method and polymerase chain reaction technique was used detect the esp, ace, and efaA virulence genes.
    Results: Out of 145 isolates, 84 (57.9%) were identified as E. faecalis and 61 (42.1%) as E. faecium. Resistance to kanamycin and quinupristin-dalfopristin was 82.1% (69/84) and 85.7% (72/84), respectively, in E. faecalis isolates. Out of 61 E. faecalis isolates, 38 (62.4%) were resistant to kanamycin. Among the E. faecalis isolates, esp was the most dominant virulence gene (73.80%), followed by efaA, and ace, which were detected in 60.71%, and 30.95% isolates, respectively. In total, 68.27% of the strains were biofilm producers. Further, esp and efaA genes were more frequently found among E. faecalis strains with moderate and strong biofilm biomass.
    Conclusions: According to the findings of our study, enterococci strains isolated from different samples possess distinctive patterns of virulence genes. The esp, ace, and efaA genes were more prevalent among E. faecalis than E. faecium. Besides, the high level antibiotic resistance of normal flora and environmental enterococci strains is alarming the researchers.
    MeSH term(s) Humans ; Anti-Bacterial Agents/pharmacology ; Cross-Sectional Studies ; Drug Resistance, Bacterial/genetics ; Virulence/genetics ; Kanamycin ; Biofilms
    Chemical Substances Anti-Bacterial Agents ; Kanamycin (59-01-8)
    Language English
    Publishing date 2024-03-04
    Publishing country England
    Document type Journal Article
    ZDB-ID 2041550-3
    ISSN 1471-2334 ; 1471-2334
    ISSN (online) 1471-2334
    ISSN 1471-2334
    DOI 10.1186/s12879-024-09117-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Overuse of computed tomography for mild head injury: A systematic review and meta-analysis.

    Saran, Maryam / Arab-Zozani, Morteza / Behzadifar, Meysam / Gholami, Mehrdad / Azari, Samad / Bragazzi, Nicola Luigi / Behzadifar, Masoud

    PloS one

    2024  Volume 19, Issue 1, Page(s) e0293558

    Abstract: Background: Computed tomography (CT) scan is a common imaging technique used to evaluate the severity of a head injury. The overuse of diagnostic interventions in the health system is a growing concern worldwide. Objectives: The aim of this systematic ... ...

    Abstract Background: Computed tomography (CT) scan is a common imaging technique used to evaluate the severity of a head injury. The overuse of diagnostic interventions in the health system is a growing concern worldwide. Objectives: The aim of this systematic review is to investigate the rate of CT scan overuse in cases of mild head injury.
    Methods: Eligibility criteria: We encompassed observational studies-either designed as cohort, case-control, or cross-sectional investigations-that reported on CT scan overuse rates for mild head injuries. Studies had to be published in peer-reviewed, English-language sources and provide full content access Information sources: Web of Sciences, Scopus, Medline via PubMed, the Cochrane Library and Embase were searched from inception until April 1, 2023. Studies were included if reporting the overuse of CT scans for mild head injuries using validated criteria. Risk of bias: We used the Risk Of Bias In Non-randomised Studies - of Interventions (ROBINS-I) tool to evaluate the risk bias assessment of included studies. Two independent reviewers evaluated the eligibility of studies, extracted data, and assessed study quality by using the Newcastle-Ottawa Scale. Synthesis of results: Overuse estimates were calculated using a random-effects model. Subgroup analyses were performed to investigate any sources of heterogeneity. Point rate of overuse of CT scans for mild head injuries was the main outcome measured as percentage point estimates with corresponding 95% CIs.
    Results: Included studies: Of the 913 potentially relevant studies identified, eight studies were selected for the final analysis. Synthesis of results: The pooled rate of CT scan overuse in patients with mild head injury was found to be 27% [95% CI: 16-43; I2 = 99%]. The rate of CT scan overuse in mild head injury cases varied depending on the criteria used. The rate of CT scan overuse was 37% [95% CI: 32-42; I2 = 0%] with the Glasgow Coma Scale (GCS), 30% [95% CI: 16-49; I2 = 99%] with the Canadian computed tomography head rule, and 10% [95% CI: 8-14; I2 = 0%] with the Pediatric Emergency Care Applied Research Network criterion (PERCAN). Based on subgroup analyses, the rate of CT scan overuse in mild head injury cases was observed to be 30% with the Canadian computed tomography head rule criterion, 43% with the National Institute for Health and Clinical Excellence criterion, and 18% with the New Orleans criterion.
    Conclusion: Limitations of evidence: The restricted number of included studies may impact generalizability. High heterogeneity was observed, leading to subgroup analyses based on age, assessment criteria, and study region. Absent data on overuse causes hinders drawing conclusions on contributing factors. Furthermore, this study solely addressed overuse rates, not associated harm or benefits. Interpretation: The overuse of CT scans in mild head injury patients is concerning, as it can result in unnecessary radiation exposure and higher healthcare costs. Clinicians and policymakers should prioritize the implementation of guidelines to reduce unnecessary radiation exposure, healthcare costs, and potential harm to patients.
    Trial registration: The study protocol of this review was registered in PROSPERO under the identification code CRD42023416080. https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023416080.
    MeSH term(s) Humans ; Child ; Cross-Sectional Studies ; Canada ; Tomography, X-Ray Computed ; Glasgow Coma Scale ; Craniocerebral Trauma/diagnostic imaging
    Language English
    Publishing date 2024-01-11
    Publishing country United States
    Document type Meta-Analysis ; Systematic Review ; Journal Article
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0293558
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Calculation of Photoneutron Contamination of Varian Linac in ICRU Soft-Tissue Phantom Using MCNPX Code.

    Cheraghian, Mojtaba / Pourfallah, Tayyeb / Sabouri-Dodaran, Amir Abbas / Gholami, Mehrdad

    Journal of medical physics

    2021  Volume 46, Issue 2, Page(s) 116–124

    Abstract: Purpose: The aim of this research was to calculate the fluence, dose equivalent (DE), and kerma of thermal, epithermal and fast photoneutrons separately, within ICRU soft-tissue-equivalent phantom in the radiotherapy treatment room, using MCNPX Monte ... ...

    Abstract Purpose: The aim of this research was to calculate the fluence, dose equivalent (DE), and kerma of thermal, epithermal and fast photoneutrons separately, within ICRU soft-tissue-equivalent phantom in the radiotherapy treatment room, using MCNPX Monte Carlo code.
    Materials and methods: For this purpose, 18 MV Varian Linac 2100 C/D machine was simulated and desired quantities were calculated on the central axis and transverse directions at different depths.
    Results: Maximum fluence, DE and kerma of total photoneutrons on central axis of the phantom were 43.8 n.cm
    Conclusion: According to this research, within the phantom; variation of fluence, DE and kerma in transverse direction were mild, and along the central axis at shallow area were sharp. DE of fast photoneutrons at shallow and deep areas were one order of magnitude greater than thermal photoneutrons.
    Language English
    Publishing date 2021-08-07
    Publishing country India
    Document type Journal Article
    ZDB-ID 1193902-3
    ISSN 1998-3913 ; 0971-6203
    ISSN (online) 1998-3913
    ISSN 0971-6203
    DOI 10.4103/jmp.JMP_40_21
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Precipitation isotopic information: A tool for building the data base to verify milk geographical origin traceability

    Behkami, Shima / Gholami, Mehrdad / Gholami, Rima / Roohparvar, Rasool

    Food control. 2020 Jan., v. 107

    2020  

    Abstract: Isotopic composition of milk is affected by various environmental factors one of which is precipitation. Therefore, an attempt was made to investigate the effectiveness of precipitation isotopic information in order to build milk geographical origin data ...

    Abstract Isotopic composition of milk is affected by various environmental factors one of which is precipitation. Therefore, an attempt was made to investigate the effectiveness of precipitation isotopic information in order to build milk geographical origin data base. It was observed that there was significantly high correlation between δ2H data and δ18O data of milk with that of the precipitation as well as correlations between C and N content in the milk with that of latitude which enables clustering of milk samples based on their geographical origin. Meanwhile, H and O isotopic data were used as a potential tool for building database for geographical traceability of milk. As a conclusion, by applying factorial analysis a clear separation based on region of origin was observed which enables the grouping of samples in two clusters, namely north and south.
    Keywords databases ; environmental factors ; factor analysis ; latitude ; milk ; milk composition ; nitrogen content ; oxygen ; provenance ; stable isotopes ; traceability
    Language English
    Dates of publication 2020-01
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1027805-9
    ISSN 0956-7135
    ISSN 0956-7135
    DOI 10.1016/j.foodcont.2019.106780
    Database NAL-Catalogue (AGRICOLA)

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  9. Article: The Efficacy of "Anatomical Posture Control Orthosis" on the Activity of Erector spinae Muscle, Risk of Falling, Balance Confidence, and Walking Speed in Osteoporotic Hyperkyphotic Subjects.

    Veiskarami, Masoumeh / Aminian, Gholamreza / Bahramizadeh, Mahmood / Gholami, Mehrdad / Ebrahimzadeh, Farzad / Arazpour, Mokhtar

    The archives of bone and joint surgery

    2022  Volume 10, Issue 9, Page(s) 798–805

    Abstract: Background: Osteoporosis is a silent and asymptomatic disease that leads to thoracic hyperkyphosis, which can interfere with the normal function of the paraspinal musculature and balance control. There is no evidence regarding the effect of the ... ...

    Abstract Background: Osteoporosis is a silent and asymptomatic disease that leads to thoracic hyperkyphosis, which can interfere with the normal function of the paraspinal musculature and balance control. There is no evidence regarding the effect of the anatomical posture control (APC) orthosis in older people with osteoporotic thoracic hyperkyphosis. This study aimed to examine the effects of this novel orthosis on the electromyography (EMG) of the erector spinae (ES) and balance control in this group of patients.
    Methods: In total, 22 elderly osteoporotic subjects with thoracic hyperkyphosis were enrolled in this study. The participants used the orthosis for 4 weeks. The clinical balance assessment scales assessing fall risk and surface EMG (sEMG) signals were recorded from the erector spinae muscles bilaterally before and after the use of orthosis. The marginal model was used with the generalized estimating equation analysis for investigating the effect of this orthosis on the sEMG of the paraspinal muscles and the balance control in this longitudinal study.
    Results: The normalized root mean square of sEMG of the lumbar and thoracic ES muscles reduced significantly (P<0.05), and significant improvement was observed (
    Conclusion: APC orthosis can decrease the activity of ES muscles during static standing and improve the static and dynamic balance in the hyperkyphotic osteoporotic subjects.
    Language English
    Publishing date 2022-10-05
    Publishing country Iran
    Document type Journal Article
    ZDB-ID 2782053-1
    ISSN 2345-461X ; 2345-4644
    ISSN (online) 2345-461X
    ISSN 2345-4644
    DOI 10.22038/ABJS.2021.53771.2678
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Evaluation of the Presence of Bacterial and Viral Agents in the Semen of Infertile Men: A Systematic and Meta-Analysis Review Study.

    Gholami, Mehrdad / Moosazadeh, Mahmood / Haghshenash, Mohammad Reza / Jafarpour, Hamed / Mousavi, Tahoora

    Frontiers in medicine

    2022  Volume 9, Page(s) 835254

    Abstract: Objectives: Infections in the male genitourinary system with bacterial and viral agents may play a significant role in male infertility. These agents usually infect the urethra, seminal vesicles, prostate, epididymis, vas deferens, and testes retrograde ...

    Abstract Objectives: Infections in the male genitourinary system with bacterial and viral agents may play a significant role in male infertility. These agents usually infect the urethra, seminal vesicles, prostate, epididymis, vas deferens, and testes retrograde through the reproductive system. A meta-analysis review study was performed to evaluate the presence of bacterial and viral agents in the semen of infertile men and its correlation with infertility.
    Methods: Relevant cross-sectional and/or case-control studies were found by an online review of national and international databases (Web of Science, PubMed, Scopus, Science Direct, and Google scholar), and suitable studies were selected. A checklist determined the qualities of all studies. Heterogeneity assay among the primary studies was evaluated by Cochran's
    Results: Seventy-two studies were included in this meta-analysis. Publication bias was compared with Egger's test, and the impact of each research on overall estimate was evaluated by sensitivity analysis. In 56 studies, the rate of bacterial infections in the semen of infertile men was 12% [95% confidence interval (CI): 10-13]. Also, in 26 case-control studies, the association of infertility in men with bacterial infections was evaluated. The results show that the odds ratio of infertility in men exposed to bacterial infections is 3.31 times higher than that in non-infected men (95% CI: 2.60-4.23). Besides, in 9 studies that examined the prevalence of human papillomavirus (HPV), herpes simplex virus 1 (HSV1), herpes simplex virus 2 (HSV2), and herpes simplex virus 1-2 (HSV1-2) in infertile men, the frequency of these viruses was 15% (95% CI: 9-21). In 6 case-control studies, the association between human cytomegalovirus (HCMV), Cytomegalovirus (CMV), and HPV and male infertility was evaluated. The chance of male infertility due to exposure to these viruses was 2.24 times higher than those without exposure to these viruses (CI 95%: 1.9-4.52). The results show that the chance of infertility in men exposed to bacteria was significantly higher than that in the uninfected population.
    Conclusion: This meta-analysis showed that viral and bacterial infections are a risk factor and could impair male fertility potential. Moreover, our study supports the hypothesis that bacterial and viral infections of the genital tract correlate positively with impairment of sperm quality in the male population.
    Language English
    Publishing date 2022-05-04
    Publishing country Switzerland
    Document type Systematic Review
    ZDB-ID 2775999-4
    ISSN 2296-858X
    ISSN 2296-858X
    DOI 10.3389/fmed.2022.835254
    Database MEDical Literature Analysis and Retrieval System OnLINE

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