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  1. Article ; Online: Comparative studies on measurement of membrane potential of bacterial cells treated with ZnO nanoparticles by Spectrofluorometry, fluorescence microscopy and flowcytometry.

    Khater, Maya / Khater, Sagar S / Gholap, Haribhau / Patil, Rajendra / Kulkarni, Gauri

    Journal of microbiological methods

    2020  Volume 173, Page(s) 105920

    Abstract: Many methods are developed to assess antimicrobial action of ZnO nanoparticles (NPs). A large number of methods associated with the use of fluorescent probes are developed, including Spectrofluorometry, fluorescence microscopy, and cytometry. In this ... ...

    Abstract Many methods are developed to assess antimicrobial action of ZnO nanoparticles (NPs). A large number of methods associated with the use of fluorescent probes are developed, including Spectrofluorometry, fluorescence microscopy, and cytometry. In this study, flowcytometry, Spectrofluorometry and fluorescent microscopy was used to measure membrane potential variation of E. coli and S. aureus cells treated with two different sizes of zinc oxide (ZnO) NPs and were compared with conventional methods. In order to estimate change in membrane potential, E. coli and S. aureus cells were treated with iopnophore agent carbonyl cyanide m-chlorophenylhydrazone (CCCP) and membrane potential was evaluated using fluorescent probe 3,3'-Diethyloxacarbocyanine, iodide (DIOC
    MeSH term(s) Anti-Bacterial Agents ; Bacteria/drug effects ; Bacteria/metabolism ; Escherichia coli ; Flow Cytometry/methods ; Membrane Potentials/physiology ; Microbial Sensitivity Tests ; Microscopy, Fluorescence/methods ; Nanoparticles/chemistry ; Reactive Oxygen Species/metabolism ; Spectrometry, Fluorescence/methods ; Staphylococcus aureus ; Zinc Oxide/pharmacology
    Chemical Substances Anti-Bacterial Agents ; Reactive Oxygen Species ; Zinc Oxide (SOI2LOH54Z)
    Language English
    Publishing date 2020-04-15
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 604916-3
    ISSN 1872-8359 ; 0167-7012
    ISSN (online) 1872-8359
    ISSN 0167-7012
    DOI 10.1016/j.mimet.2020.105920
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1849: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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  2. Article: Hierarchical nanostructures of Au@ZnO: antibacterial and antibiofilm agent

    Gholap, Haribhau / Sambhaji Warule / Jaiprakash Sangshetti / Gauri Kulkarni / Arun Banpurkar / Surekha Satpute / Rajendra Patil

    Applied microbiology and biotechnology. 2016 July, v. 100, no. 13

    2016  

    Abstract: The perpetual use of antibiotics against pathogens inadvertently altered their genes that have translated into an unprecedented resistance in microorganisms in the twenty-first century. Many researchers have formulated bactericidal and bacteriostatic ... ...

    Abstract The perpetual use of antibiotics against pathogens inadvertently altered their genes that have translated into an unprecedented resistance in microorganisms in the twenty-first century. Many researchers have formulated bactericidal and bacteriostatic inorganic nanoparticle-based antiseptics that may be linked to broad-spectrum activity and far lower propensity to induce microbial resistance than organic-based antibiotics. Based on this line, herein, we present observations on microbial abatement using gold-based zinc oxide nanostructures (Au@ZnO) which are synthesized using hydrothermal route. Inhibition of microbial growth and biofilm using Au@ZnO is a unique feature of our study. Furthermore, this study evinces antimicrobial and antibiofilm mechanisms of photo-eradiated Au@ZnO by disruption of cellular functions and biofilms via reactive oxygen species (ROS)-dependent generation of superoxide anion radical. The present study is significant as it introduces novel functionalities to Au@ZnO in the biomedical field which can be extended to other species of microbial pathogens.
    Keywords antibiotics ; antiseptics ; biofilm ; genes ; microbial growth ; microorganisms ; nanomaterials ; pathogens ; superoxide anion ; translation (genetics) ; zinc oxide
    Language English
    Dates of publication 2016-07
    Size p. 5849-5858.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    ZDB-ID 392453-1
    ISSN 1432-0614 ; 0171-1741 ; 0175-7598
    ISSN (online) 1432-0614
    ISSN 0171-1741 ; 0175-7598
    DOI 10.1007/s00253-016-7391-1
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    In stock of ZB MED Bonn / Germany

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  3. Article ; Online: Hierarchical nanostructures of Au@ZnO: antibacterial and antibiofilm agent.

    Gholap, Haribhau / Warule, Sambhaji / Sangshetti, Jaiprakash / Kulkarni, Gauri / Banpurkar, Arun / Satpute, Surekha / Patil, Rajendra

    Applied microbiology and biotechnology

    2016  Volume 100, Issue 13, Page(s) 5849–5858

    Abstract: The perpetual use of antibiotics against pathogens inadvertently altered their genes that have translated into an unprecedented resistance in microorganisms in the twenty-first century. Many researchers have formulated bactericidal and bacteriostatic ... ...

    Abstract The perpetual use of antibiotics against pathogens inadvertently altered their genes that have translated into an unprecedented resistance in microorganisms in the twenty-first century. Many researchers have formulated bactericidal and bacteriostatic inorganic nanoparticle-based antiseptics that may be linked to broad-spectrum activity and far lower propensity to induce microbial resistance than organic-based antibiotics. Based on this line, herein, we present observations on microbial abatement using gold-based zinc oxide nanostructures (Au@ZnO) which are synthesized using hydrothermal route. Inhibition of microbial growth and biofilm using Au@ZnO is a unique feature of our study. Furthermore, this study evinces antimicrobial and antibiofilm mechanisms of photo-eradiated Au@ZnO by disruption of cellular functions and biofilms via reactive oxygen species (ROS)-dependent generation of superoxide anion radical. The present study is significant as it introduces novel functionalities to Au@ZnO in the biomedical field which can be extended to other species of microbial pathogens.
    MeSH term(s) Anti-Bacterial Agents/chemistry ; Anti-Bacterial Agents/pharmacology ; Biofilms/drug effects ; Gold/chemistry ; Gold/pharmacology ; Microbial Sensitivity Tests ; Nanostructures/chemistry ; Nanostructures/microbiology ; Nanostructures/toxicity ; Staphylococcus aureus/drug effects ; Staphylococcus aureus/physiology ; Zinc Oxide/chemistry ; Zinc Oxide/pharmacology
    Chemical Substances Anti-Bacterial Agents ; Gold (7440-57-5) ; Zinc Oxide (SOI2LOH54Z)
    Language English
    Publishing date 2016-07
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 392453-1
    ISSN 1432-0614 ; 0171-1741 ; 0175-7598
    ISSN (online) 1432-0614
    ISSN 0171-1741 ; 0175-7598
    DOI 10.1007/s00253-016-7391-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2229: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Fluorescent cadmium telluride quantum dots embedded chitosan nanoparticles: a stable, biocompatible preparation for bio-imaging.

    Ghormade, Vandana / Gholap, Haribhau / Kale, Sonia / Kulkarni, Vaishnavi / Bhat, Suresh / Paknikar, Kishore

    Journal of biomaterials science. Polymer edition

    2015  Volume 26, Issue 1, Page(s) 42–56

    Abstract: Fluorescent cadmium telluride quantum dots (CdTe QDs) are an optically attractive option for bioimaging, but are known to display high cytotoxicity. Nanoparticles synthesized from chitosan, a natural biopolymer of β 1-4 linked glucosamine, display good ... ...

    Abstract Fluorescent cadmium telluride quantum dots (CdTe QDs) are an optically attractive option for bioimaging, but are known to display high cytotoxicity. Nanoparticles synthesized from chitosan, a natural biopolymer of β 1-4 linked glucosamine, display good biocompatibility and cellular uptake. A facile, green synthetic strategy has been developed to embed green fluorescent cadmium telluride quantum dots (CdTe QDs) in biocompatible CNPs to obtain a safer preparation than 'as is' QDs. High-resolution transmission electron microscopy showed the crystal lattice corresponding to CdTe QDs embedded in CNPs while thermogravimetry confirmed their polymeric composition. Electrostatic interactions between thiol-capped QDs (4 nm, -57 mV) and CNPs (~300 nm, +38 mV) generated CdTe QDs-embedded CNPs that were stable up to three months. Further, viability of NIH3T3 mouse fibroblast cells in vitro increased in presence of QDs-embedded CNPs as compared to bare QDs. At the highest concentration (10 μg/ml), the former shows 34 and 39% increase in viability at 24 and 48 h, respectively, as compared to the latter. This shows that chitosan nanoparticles do not release the QDs up to 48 h and do not cause extended toxicity. Furthermore, hydrolytic enzymes such as lysozyme and chitinase did not degrade chitosan nanoparticles. Moreover, QDs-embedded CNPs show enhanced internalization in NIH3T3 cells as compared to bare QDs. This method offers ease of synthesis and handling of stable, luminescent, biocompatible CdTe QDs-embedded CNPs with a favorable toxicity profile and better cellular uptake with potential for bioimaging and targeted detection of cellular components.
    MeSH term(s) Animals ; Biocompatible Materials/chemistry ; Biocompatible Materials/metabolism ; Biological Transport ; Cadmium Compounds/chemistry ; Chitosan/chemistry ; Chitosan/metabolism ; Drug Stability ; Fluorescent Dyes/chemistry ; Fluorescent Dyes/metabolism ; Mice ; Molecular Imaging/methods ; NIH 3T3 Cells ; Optical Phenomena ; Quantum Dots/chemistry ; Solubility ; Solvents/chemistry ; Tellurium/chemistry ; Water/chemistry
    Chemical Substances Biocompatible Materials ; Cadmium Compounds ; Fluorescent Dyes ; Solvents ; Water (059QF0KO0R) ; Chitosan (9012-76-4) ; Tellurium (NQA0O090ZJ) ; cadmium telluride (STG188WO13)
    Language English
    Publishing date 2015
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1014190-x
    ISSN 1568-5624 ; 1568-5616 ; 0920-5063
    ISSN (online) 1568-5624 ; 1568-5616
    ISSN 0920-5063
    DOI 10.1080/09205063.2014.982240
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2931: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article: In situ fabrication of highly crystalline CdS decorated Bi2S3 nanowires (nano-heterostructure) for visible light photocatalyst application

    Panmand, Rajendra P / Sethi, Yogesh A / Deokar, Rajashree S / Late, Datta J / Gholap, Haribhau M / Baeg, Jin-Ook / Kale, Bharat B

    RSC advances. 2016 Mar. 01, v. 6, no. 28

    2016  

    Abstract: In situ synthesis of the orthorhombic Bi2S3 nanowires decorated with hexagonal CdS nanoparticles (nano-heterostructure) has been demonstrated by a facile solvothermal method. The tiny 5–7 nm CdS spherical nanoparticles are decorated on the surfaces of 30– ...

    Abstract In situ synthesis of the orthorhombic Bi2S3 nanowires decorated with hexagonal CdS nanoparticles (nano-heterostructure) has been demonstrated by a facile solvothermal method. The tiny 5–7 nm CdS spherical nanoparticles are decorated on the surfaces of 30–40 nm Bi2S3 nanowires, successfully. Structural, morphological and optical studies clearly show the existence of CdS on the nanowires. A possible sequential deposition growth mechanism is proposed on the basis of experimental results to reveal the formation of the nano heterostructure. The heterostructures have been used as a photocatalyst for hydrogen production as well as degradation of methylene blue under solar light. The maximum hydrogen evolution i.e. 4560 and 2340 μmol h−1 0.5 g was obtained from H2S splitting and glycerol degradation for Bi2S3 NWs decorated with CdS nanoparticles (nano-heterostructure) which is higher than that of the Bi2S3 NWs (3000 and 1170 μmol h−1 0.5 g, respectively). The enhanced photocatalytical hydrogen evolution efficiency of the heterostructures is mainly attributed to its nanostructure. In the nano heterostructure, the CdS nanoparticles control the charge carrier transition, recombination, and separation, while the Bi2S3 nanowire serves as a support for the CdS nanoparticles. The photogenerated electron's migration is faster than the holes from the inside of a CdS nanoparticle to its surface or to the phase interface, resulting in a relatively higher hole density inside the CdS nanoparticle leaving electron density at surface of the Bi2S3 NWs. This influences the photocatalytic activity under solar light. Such nano-heterostructures may have potential in other photocatalytic reactions.
    Keywords glycerol ; hydrogen production ; hydrogen sulfide ; light ; methylene blue ; nanoparticles ; nanowires ; photocatalysis ; photocatalysts ; solar radiation
    Language English
    Dates of publication 2016-0301
    Size p. 23508-23517.
    Publishing place The Royal Society of Chemistry
    Document type Article
    ISSN 2046-2069
    DOI 10.1039/c6ra01488g
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  6. Article ; Online: CdTe-TiO2 nanocomposite: an impeder of bacterial growth and biofilm.

    Gholap, Haribhau / Patil, Rajendra / Yadav, Prasad / Banpurkar, Arun / Ogale, Satishchandra / Gade, Wasudeo

    Nanotechnology

    2013  Volume 24, Issue 19, Page(s) 195101

    Abstract: The resurgence of infectious diseases and associated issues related to antibiotic resistance has raised enormous challenges which may possibly be confronted primarily by nanotechnology routes. One key need of critical significance in this context is the ... ...

    Abstract The resurgence of infectious diseases and associated issues related to antibiotic resistance has raised enormous challenges which may possibly be confronted primarily by nanotechnology routes. One key need of critical significance in this context is the development of an agent capable of inhibiting quorum sensing mediated biofilm formation in pathogenic organisms. In this work we examine the possible use of a nanocomposite, CdTe-TiO2, as an impeder of growth and biofilm. In the presence of CdTe-TiO2, scanning electron microscopy (SEM) analysis shows exposed cells without the surrounding matrix. Confocal laser scanning microscopy shows spatially distributed fluorescence, a typical indication of an impeded biofilm, as opposed to the control which shows matrix-covered cells and continuous fluorescence, typical of biofilm formation. Quantitatively, the inhibition of biofilm was ∼57%. CdTe-TiO2 also exhibits good antibacterial properties against Gram positive and Gram negative organisms by virtue of the generation of reactive oxygen species inside the cells, reflected by a ruptured appearance in the SEM analysis.
    MeSH term(s) Anti-Bacterial Agents/chemistry ; Anti-Bacterial Agents/pharmacology ; Bacillus subtilis/cytology ; Bacillus subtilis/drug effects ; Bacillus subtilis/physiology ; Bacterial Infections/drug therapy ; Biofilms/drug effects ; Cadmium Compounds/chemistry ; Cadmium Compounds/pharmacology ; Escherichia coli/cytology ; Escherichia coli/drug effects ; Escherichia coli/physiology ; Humans ; Nanocomposites/chemistry ; Nanocomposites/ultrastructure ; Pseudomonas aeruginosa/cytology ; Pseudomonas aeruginosa/drug effects ; Pseudomonas aeruginosa/physiology ; Quantum Dots ; Quorum Sensing/drug effects ; Tellurium/chemistry ; Tellurium/pharmacology ; Titanium/chemistry ; Titanium/pharmacology
    Chemical Substances Anti-Bacterial Agents ; Cadmium Compounds ; titanium dioxide (15FIX9V2JP) ; Titanium (D1JT611TNE) ; Tellurium (NQA0O090ZJ) ; cadmium telluride (STG188WO13)
    Language English
    Publishing date 2013-05-17
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1362365-5
    ISSN 1361-6528 ; 0957-4484
    ISSN (online) 1361-6528
    ISSN 0957-4484
    DOI 10.1088/0957-4484/24/19/195101
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Magnetite/CdTe magnetic-fluorescent composite nanosystem for magnetic separation and bio-imaging.

    Kale, Anup / Kale, Sonia / Yadav, Prasad / Gholap, Haribhau / Pasricha, Renu / Jog, J P / Lefez, Benoit / Hannoyer, Béatrice / Shastry, Padma / Ogale, Satishchandra

    Nanotechnology

    2011  Volume 22, Issue 22, Page(s) 225101

    Abstract: A new synthesis protocol is described to obtain a CdTe decorated magnetite bifunctional nanosystem via dodecylamine (DDA) as cross linker. High resolution transmission electron microscopy (HRTEM), energy-dispersive x-ray spectroscopy (EDAX), vibrating ... ...

    Abstract A new synthesis protocol is described to obtain a CdTe decorated magnetite bifunctional nanosystem via dodecylamine (DDA) as cross linker. High resolution transmission electron microscopy (HRTEM), energy-dispersive x-ray spectroscopy (EDAX), vibrating sample magnetometry (VSM), Fourier transform infrared spectroscopy (FTIR), diffuse reflectance spectroscopy (DRS) and fluorescence microscopy are used to characterize the constitution, size, composition and physical properties of these superparamagnetic-fluorescent nanoparticles. These CdTe decorated magnetite nanoparticles were then functionalized with anti-epidermal growth factor receptor (EGFR) antibody to specifically target cells expressing this receptor. The EGFR is a transmembrane glycoprotein and is expressed on tumor cells from different tissue origins including human leukemic cell line Molt-4 cells. The magnetite-CdTe composite nanosystem is shown to perform excellently for specific selection, magnetic separation and fluorescent detection of EGFR positive Molt-4 cells from a mixed population. Flow cytometry and confocal laser scanning microscopy results show that this composite nanosystem has great potential in antibody functionalized magnetic separation and imaging of cells using cell surface receptor antibody.
    MeSH term(s) Cadmium Compounds/chemistry ; Cell Line, Tumor ; Cell Survival ; Contrast Media/chemistry ; Humans ; Immunomagnetic Separation/methods ; Luminescence ; Magnetite Nanoparticles/chemistry ; Magnetite Nanoparticles/ultrastructure ; Microscopy, Confocal/methods ; Neoplasms/diagnosis ; Receptor, Epidermal Growth Factor/metabolism ; Spectroscopy, Fourier Transform Infrared ; Tellurium/chemistry ; Thermogravimetry ; X-Ray Diffraction
    Chemical Substances Cadmium Compounds ; Contrast Media ; Magnetite Nanoparticles ; Receptor, Epidermal Growth Factor (EC 2.7.10.1) ; Tellurium (NQA0O090ZJ) ; cadmium telluride (STG188WO13)
    Language English
    Publishing date 2011-06-03
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1362365-5
    ISSN 1361-6528 ; 0957-4484
    ISSN (online) 1361-6528
    ISSN 0957-4484
    DOI 10.1088/0957-4484/22/22/225101
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Hierarchical nanostructures of Au@ZnO: antibacterial and antibiofilm agent

    Gholap, Haribhau / Sambhaji Waruleauthor / Jaiprakash Sangshettiauthor / Gauri Kulkarniauthor / Arun Banpurkarauthor / Surekha Satputeauthor / Rajendra Patilauthor
    Language English
    Document type Article
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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