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  1. Article ; Online: Topical Spray of dsRNA Induces Mortality and Inhibits Chilli Leaf Curl Virus Transmission by

    Chakraborty, Prosenjit / Ghosh, Amalendu

    Cells

    2022  Volume 11, Issue 5

    Abstract: Chilli leaf curl ... ...

    Abstract Chilli leaf curl virus
    MeSH term(s) Animals ; Asia ; Begomovirus/physiology ; HSP70 Heat-Shock Proteins/genetics ; Hemiptera ; RNA, Double-Stranded/pharmacology ; RNA, Messenger
    Chemical Substances HSP70 Heat-Shock Proteins ; RNA, Double-Stranded ; RNA, Messenger
    Language English
    Publishing date 2022-02-28
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells11050833
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Rapid and zero-cost DNA extraction from soft-bodied insects for routine PCR-based applications.

    Jangra, Sumit / Ghosh, Amalendu

    PloS one

    2022  Volume 17, Issue 7, Page(s) e0271312

    Abstract: Nucleic acid extraction is the first and foremost step in molecular biology studies. Extraction of DNA from small, soft-bodied insects is often time-consuming and costly. A fast, easy, and cost-effective DNA extraction method with greater yield and ... ...

    Abstract Nucleic acid extraction is the first and foremost step in molecular biology studies. Extraction of DNA from small, soft-bodied insects is often time-consuming and costly. A fast, easy, and cost-effective DNA extraction method with greater yield and purity of DNA would aid in the rapid diagnostics, screening of large populations, and other routine PCR-based applications. The present study evaluated and standardized a rapid and zero-cost DNA extraction from soft-bodied small insects for routine molecular studies. Five rapid DNA extraction methods viz. extraction in sterile distilled water (SDW), 1X phosphate-buffered saline (PBS, pH 7.4), 1.4 M sodium chloride (NaCl), 20 mM ethylenediaminetetraacetic acid (EDTA, pH 8.0), and elution from blotted nitrocellulose membrane (NCM) were compared with standard CTAB extraction buffer and DNeasy® Blood and Tissue Kit methods. The average yield, purity, storage stability, time, and cost of extraction were assessed for all the methods and compared. A method of DNA extraction by simply crushing the soft-bodied insects in SDW was ideal in terms of yield, purity, storability, and performing routine PCR-based applications including detection of pathogens from vector species. The extraction could be accomplished in 2.5 min only with zero-reagent cost. The method would be useful in rapid molecular diagnostics and screening large populations of soft-bodied insects.
    MeSH term(s) Animals ; DNA/genetics ; Insecta/genetics ; Nucleic Acid Amplification Techniques ; Nucleic Acids ; Polymerase Chain Reaction/methods
    Chemical Substances Nucleic Acids ; DNA (9007-49-2)
    Language English
    Publishing date 2022-07-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0271312
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Effect of silencing

    Thesnim, Pathukandathil / Jangra, Sumit / Kumar, Manish / Ghosh, Amalendu

    Frontiers in plant science

    2023  Volume 14, Page(s) 1136262

    Abstract: ... Bemisia ... ...

    Abstract Bemisia tabaci
    Language English
    Publishing date 2023-03-14
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2023.1136262
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  4. Article: Effect of silencing

    Rajesh, Vavilapalli / Jangra, Sumit / Ghosh, Amalendu

    Frontiers in microbiology

    2023  Volume 14, Page(s) 1254246

    Abstract: ... Thrips ... ...

    Abstract Thrips palmi
    Language English
    Publishing date 2023-10-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2023.1254246
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Diversity of transgenes in sustainable management of insect pests.

    Rakesh, V / Kalia, Vinay K / Ghosh, Amalendu

    Transgenic research

    2023  Volume 32, Issue 5, Page(s) 351–381

    Abstract: Insecticidal transgenes, when incorporated and expressed in plants, confer resistance against insects by producing several products having insecticidal properties. Protease inhibitors, lectins, amylase inhibitors, and chitinase genes are associated with ... ...

    Abstract Insecticidal transgenes, when incorporated and expressed in plants, confer resistance against insects by producing several products having insecticidal properties. Protease inhibitors, lectins, amylase inhibitors, and chitinase genes are associated with the natural defenses developed by plants to counter insect attacks. Several toxin genes are also derived from spiders and scorpions for protection against insects. Bacillus thuringiensis Berliner is a microbial source of insecticidal toxins. Several methods have facilitated the large-scale production of transgenic plants. Bt-derived cry, cyt, vip, and sip genes, plant-derived genes such as lectins, protease inhibitors, and alpha-amylase inhibitors, insect cell wall-degrading enzymes like chitinase and some proteins like arcelins, plant defensins, and ribosome-inactivating proteins have been successfully utilized to impart resistance to insects. Besides, transgenic plants expressing double-stranded RNA have been developed with enhanced resistance. However, the long-term effects of transgenes on insect resistance, the environment, and human health must be thoroughly investigated before they are made available for commercial planting. In this chapter, the present status, prospects, and future scope of transgenes for insect pest management have been summarized and discussed.
    MeSH term(s) Animals ; Humans ; Insecta/genetics ; Insecticides/metabolism ; Transgenes ; Plants, Genetically Modified/genetics ; Plants, Genetically Modified/metabolism ; Bacillus thuringiensis/genetics ; Bacillus thuringiensis/metabolism ; Protease Inhibitors/metabolism ; Protease Inhibitors/pharmacology ; Lectins/genetics ; Chitinases/genetics ; Bacterial Proteins/metabolism ; Endotoxins/pharmacology ; Hemolysin Proteins/genetics ; Pest Control, Biological
    Chemical Substances Insecticides ; Protease Inhibitors ; Lectins ; Chitinases (EC 3.2.1.14) ; Bacterial Proteins ; Endotoxins ; Hemolysin Proteins
    Language English
    Publishing date 2023-08-12
    Publishing country Netherlands
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 31620-9
    ISSN 1573-9368 ; 0962-8819
    ISSN (online) 1573-9368
    ISSN 0962-8819
    DOI 10.1007/s11248-023-00362-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3600: Show issues Location:
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  6. Article ; Online: Mitochondrial genetic diversity of Thrips tabaci (Thysanoptera: Thripidae) in onion growing regions of the United States.

    Iftikhar, Romana / Ghosh, Amalendu / Pappu, Hanu R

    Journal of economic entomology

    2023  Volume 116, Issue 3, Page(s) 1025–1032

    Abstract: Onion thrips (Thrips tabaci Lindeman, Thysanoptera: Thripidae) causes severe damage to many horticultural and agronomic crops worldwide. It also acts as a vector of several plant viruses. T. tabaci is a key pest of Allium cepa in the United States. ... ...

    Abstract Onion thrips (Thrips tabaci Lindeman, Thysanoptera: Thripidae) causes severe damage to many horticultural and agronomic crops worldwide. It also acts as a vector of several plant viruses. T. tabaci is a key pest of Allium cepa in the United States. However, there is limited information available on the genetic variation within and between T. tabaci populations in the United States and its key evolutionary parameters. In the current study, 83 T. tabaci specimens were collected from A. cepa from 15 different locations comprising four states of the United States. A total of 92 mtCOI gene sequences of T. tabaci from A. cepa were analyzed to understand the genetic diversity and structure of T. tabaci collected from onion host. Seven distinct haplotypes of T. tabaci infesting A. cepa were identified from the current collection, while nine T. tabaci sequences retrieved from GenBank comprised 5 haplotypes. Overall, 15 haplotypes of T. tabaci infesting A. cepa were identified in the world that includes the ten haplotypes in the United States. In the phylogenetic analysis, all the populations collected during the study clustered with thelytokous lineage, while T. tabaci sequences retrieved from GenBank corresponded to leek-associated arrhenotokous lineage. The highest genetic variation was found in Elba and Malheur populations with 3 haplotypes identified in each. The results suggest that haplotypes 1 and 7 are more frequently prevailing haplotypes in the north-western United States, with haplotype 1 being the predominant all over the country. The eastern United States appears to have a more diverse group of haplotypes. The populations from Hungary constituted distinct haplotypes and a haplotype from Kingston linked it with the predominant haplotype.
    MeSH term(s) United States ; Animals ; Onions/genetics ; Thysanoptera/genetics ; Phylogeny ; Biological Evolution ; Genetic Variation
    Language English
    Publishing date 2023-04-13
    Publishing country England
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 3031-4
    ISSN 1938-291X ; 0022-0493
    ISSN (online) 1938-291X
    ISSN 0022-0493
    DOI 10.1093/jee/toad039
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.B 1118: Show issues Location:
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  7. Article: Silencing of Thrips palmi UHRF1BP1 and PFAS Using Antisense Oligos Induces Mortality and Reduces Tospovirus Titer in Its Vector

    Priti / Mukherjee, Sunil Kumar / Ghosh, Amalendu

    Pathogens (Basel, Switzerland)

    2022  Volume 11, Issue 11

    Abstract: Thrips palmi (Thysanoptera: Thripidae) is an important pest of vegetables, legumes, and ornamentals. In addition, it transmits several plant viruses. T. palmi genes associated with innate immunity, endocytosis-related pathways, and cuticular development ... ...

    Abstract Thrips palmi (Thysanoptera: Thripidae) is an important pest of vegetables, legumes, and ornamentals. In addition, it transmits several plant viruses. T. palmi genes associated with innate immunity, endocytosis-related pathways, and cuticular development are highly enriched in response to Groundnut bud necrosis orthotospovirus (GBNV, genus Orthotospovirus, family Tospoviridae) infection. As the previous transcriptomic study suggested the involvement of T. palmi UHRF1BP1 and PFAS in GBNV infection, these two genes were targeted for silencing using antisense oligonucleotides (ASOs), and the effects on thrips’ fitness and virus acquisition were observed. Phosphorothioate modification of ASOs was carried out by replacing the nonbridging oxygen atom with a sulfur atom at the 3′ position to increase nuclease stability. The modified ASOs were delivered orally through an artificial diet. Exposure to ASOs reduced the target mRNA expression up to 2.70-fold optimally. Silencing of T. palmi UHRF1BP1 and PFAS induced 93.33% mortality that further increased up to 100% with an increase in exposure. Silencing of T. palmi UHRF1BP1 and PFAS also produced morphological deformities in the treated T. palmi. GBNV titer in T. palmi significantly declined post-exposure to ASOs. This is the first-ever report of silencing T. palmi UHRF1BP1 and PFAS using modified ASO to induce mortality and impair virus transmission in T. palmi. T. palmi UHRF1BP1 and PFAS would be novel genetic targets to manage thrips and restrict the spread of tospovirus.
    Language English
    Publishing date 2022-11-10
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens11111319
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  8. Article: Delivery of progeny virus from the infectious clone of cucumber green mottle mosaic virus and quantification of the viral load in different host plants.

    Nayaka, S Naveen / Jailani, A Abdul Kader / Ghosh, Amalendu / Roy, Anirban / Mandal, Bikash

    3 Biotech

    2023  Volume 13, Issue 6, Page(s) 209

    Abstract: Cucumber green mottle mosaic virus (CGMMV, genus : Supplementary information: The online version contains supplementary material available at 10.1007/s13205-023-03630-y. ...

    Abstract Cucumber green mottle mosaic virus (CGMMV, genus
    Supplementary information: The online version contains supplementary material available at 10.1007/s13205-023-03630-y.
    Language English
    Publishing date 2023-05-23
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2600522-0
    ISSN 2190-5738 ; 2190-572X
    ISSN (online) 2190-5738
    ISSN 2190-572X
    DOI 10.1007/s13205-023-03630-y
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  9. Article ; Online: Association of a novel begomovirus species with fenugreek yellow vein disease in India.

    Kumar, Manish / Ghosh, Amalendu / Jadon, Kuldeep Singh / Kaur, Baljeet / Kakani, Rajesh K / Solanki, Ramesh K

    Molecular biology reports

    2023  Volume 50, Issue 11, Page(s) 9203–9211

    Abstract: Background: Fenugreek (Trigonella foenum-graecum L.) is an annual medicinal and spice crop belonging to the family Fabaceae. The occurrence of a yellow vein disease was recorded in fenugreek in Jodhpur (India) in 2022. The infection of begomoviruses in ... ...

    Abstract Background: Fenugreek (Trigonella foenum-graecum L.) is an annual medicinal and spice crop belonging to the family Fabaceae. The occurrence of a yellow vein disease was recorded in fenugreek in Jodhpur (India) in 2022. The infection of begomoviruses in legume crops results in significant yield loss and major economic loss. The current study reports an association of a novel begomovirus species associated with yellow vein disease in Fenugreek.
    Methods and results: In symptomatic fenugreek plants, geminivirus-like particles were visible under a transmission electron microscope. Further, nucleotide sequence analysis of the rolling circle amplified product revealed 2743 nucleotide DNA-A genome with close relatedness to French bean leaf curl virus (88.21%) and Senna leaf curl virus (87.63%). It was proposed as a new begomovirus species, Fenugreek yellow vein Rajasthan virus. The genome organization suggested the presence of a typical nonanucleotide sequence along with 7 ORFs in DNA-A. A possible recombination event took place in the coat protein (V1) region with Pedilanthus leaf curl virus and Chilli leaf curl virus as major and minor parents. The recombinant virus poses possible threats to several other legume crops. To the best of our knowledge, this is the first report of the association of FeYVRaV with fenugreek yellow vein disease from northwestern India.
    Conclusions: In conclusion, the presence of a novel begomovirus species associated with yellow vein disease in fenugreek is alarming and needs further studies on its infectivity to prevent its spread to legume crops.
    MeSH term(s) Begomovirus/genetics ; Phylogeny ; Trigonella/genetics ; DNA, Viral/genetics ; Sequence Analysis, DNA ; India ; Plant Diseases ; Fabaceae/genetics
    Chemical Substances DNA, Viral
    Language English
    Publishing date 2023-09-30
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 186544-4
    ISSN 1573-4978 ; 0301-4851
    ISSN (online) 1573-4978
    ISSN 0301-4851
    DOI 10.1007/s11033-023-08806-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1140: Show issues Location:
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  10. Article: In silico analyses of molecular interactions between groundnut bud necrosis virus and its vector,

    Jagdale, Shounak S / Ghosh, Amalendu

    Virusdisease

    2019  Volume 30, Issue 2, Page(s) 245–251

    Abstract: Groundnut bud necrosis virus (GBNV) is an economically important tospovirus transmitted ... ...

    Abstract Groundnut bud necrosis virus (GBNV) is an economically important tospovirus transmitted by
    Language English
    Publishing date 2019-03-26
    Publishing country India
    Document type Journal Article
    ZDB-ID 2846993-8
    ISSN 2347-3517 ; 2347-3584
    ISSN (online) 2347-3517
    ISSN 2347-3584
    DOI 10.1007/s13337-019-00521-w
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