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  1. Article ; Online: Histological and Ultrastructural Studies on the Intestine of Guntea Loach, Lepidocephalichthys Guntea (Cypriniformes, Cobitidae)

    Ghosh S. K.

    Vestnik Zoologii, Vol 53, Iss 2, Pp 165-

    2019  Volume 172

    Abstract: The cellular organizations of intestine in Lepidocephalichthys guntea (Hamilton, 1822) have been described by light as well as scanning and transmission electron microscopy. The intestine is short and straight like, marked into anterior, middle and ... ...

    Abstract The cellular organizations of intestine in Lepidocephalichthys guntea (Hamilton, 1822) have been described by light as well as scanning and transmission electron microscopy. The intestine is short and straight like, marked into anterior, middle and posterior region based on mucosal folds, number and size of columnar epithelial cells and mucous cells, thickness of submucosa and muscularis layer. The mucosa of anterior intestine forms high folds, which are lined with compactly arranged columnar epithelial cells and mucous cells. In the middle intestine, folds are pointless whereas the posterior intestine is without folds. The submucosa is formed of thin layer of connective tissue, contained collagen bundles and blood capillaries, comparatively well developed in the posterior intestine. By scanning electron microscopy, outlines of the luminal surface of anterior and middle intestine is embossed with oval or rounded columnar epithelial cells contained densely packed stubby microridges. The posterior intestine has closely set longitudinal folds characterized with minute blood capillaries and columnar epithelial cells having inconspicuous microridges. Ultrastructurally, the mucosal surface of the intestine consists of mucous cells with electron dense granules and columnar epithelial cells having numerous microvilli, mitochondria, endoplasmic reticulum, lysosomes and Golgi body. Cellular components of the anterior and middle intestine participate in the absorption whereas the presence of enormous blood vessels and capillary net work of posterior intestine probably responsible for air breathing.
    Keywords lepidocephalichthys guntea ; intestine ; histoarchitecture ; fine structure ; Zoology ; QL1-991
    Subject code 610
    Language English
    Publishing date 2019-04-01T00:00:00Z
    Publisher Sciendo
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Book ; Online: EVALUATING THE POTENTIAL OF 8 BAND PLANETSCOPE DATASET FOR CROP CLASSIFICATION USING RANDOM FOREST AND GRADIENT TREE BOOSTING BY GOOGLE EARTH ENGINE

    Sharma, V. / Ghosh, S. K.

    eISSN: 2194-9034

    2023  

    Abstract: One of the challenging task in agriculture is mapping of crops using satellite images as spectral properties of the crops looks similar to each other, and there are many complexities which are there in the field such as small land holdings, heterogeneous ...

    Abstract One of the challenging task in agriculture is mapping of crops using satellite images as spectral properties of the crops looks similar to each other, and there are many complexities which are there in the field such as small land holdings, heterogeneous and often distinct field patterns. In past, mapping with high resolution satellite images was not possible due to the non-availability of data and even they were costlier. However, with the free availability of PlanetScope dataset having 8 spectral bands, with daily revisit cycle and resolution of 3m, it is now possible to carry out mapping and monitoring of agriculture crops. The objective of the study is to classify major crops of rabi (December-April) season using single date PlanetScope imagery in the Haridwar district, Uttarakhand, India. Classification of crops has been carried out using an ensemble based machine learning algorithms within Google earth engine. Off late, Google Earth Engine (GEE), a cloud based platform has attracted the attention of remote sensing analyst since it expediates the classification yielding good results of high quality. In this study, Random Forest (RF) and Gradient Tree Boosting algorithm (GTB) have been used after performing hyper-parameter tuning and results shows that overall accuracy obtained by RF and GTB are 88% and 86.5% respectively. According to the results, both classifier performed well but RF achieved 1.5% high accuracy over GTB. Analysis of the results show that highest accuracy was attained by agriculture class (wheat) while other crops class exhibited lower accuracy. In this study, RF was found to be more competent as compared to GTB in classifying the crops and PlanetScope 8 band dataset has also proved its potential in classifying crops in heterogeneous fields.
    Subject code 571
    Language English
    Publishing date 2023-04-21
    Publishing country de
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Book ; Online: IMPACT OF CLIMATE ON VEGETATION INDICES OVER RAINFED DISTRICTS OF UTTARAKHAND, INDIA

    Sharma, V. / Ghosh, S. K.

    eISSN: 2194-9034

    2022  

    Abstract: Due to a change in the landscape, the climate of Uttarakhand state is changing rapidly, impacting the weather, further affecting human beings and vegetation. Nowadays, remote sensing is a favorable tool for monitoring the vegetation condition using NDVI ... ...

    Abstract Due to a change in the landscape, the climate of Uttarakhand state is changing rapidly, impacting the weather, further affecting human beings and vegetation. Nowadays, remote sensing is a favorable tool for monitoring the vegetation condition using NDVI and EVI. Studying the relationship between vegetation and climate more extensively, it is necessary to better understand the anomaly of ecosystems with climate change. This study is carried out to evaluate the vegetation cover dynamics by establishing the association between climate parameters and vegetation indices over the rain-fed districts (Nainital, Bageshwar, Champawat, Dehradun) of Uttarakhand for the period of 20 years. In this study, Google Earth Engine (GEE) is used to extract the MODIS NDVI and EVI at 250 m spatial resolution & 16-day temporal resolution data. The climate parameters for the rain-fed district (study area) are extracted from Indian Meteorological Department (IMD) Pun website for the period from 2000 to 2020. According to the annual vegetation dynamics, the peak attained by both indices is during the monsoon season, and hence they both show identical patterns to each other. Linear Regression Analysis results show a strong impact of climate on vegetation. Both indices shows a positive correlation with climate parameters where minimum temperature and rainfall are strongly correlated with EVI. Thus, the study reveals that EVI is proven to be more appropriate indices for monitoring vegetation cover as compared to NDVI for the study area.
    Subject code 910
    Language English
    Publishing date 2022-05-30
    Publishing country de
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article: Graphene oxide coated aluminium as an efficient antibacterial surface

    Mandal, P. / Ghosh, S.K. / Grewal, H.S.

    Environmental technology & innovation. 2022 Nov., v. 28

    2022  

    Abstract: Antimicrobial coatings on metallic surfaces are rapidly emerging to combat bacterial pathogens. The excess use of conventional antibiotics increases the number of resistant strains at an alarming rate, which in turn leads to detrimental implications in ... ...

    Abstract Antimicrobial coatings on metallic surfaces are rapidly emerging to combat bacterial pathogens. The excess use of conventional antibiotics increases the number of resistant strains at an alarming rate, which in turn leads to detrimental implications in the healthcare sector. Hence, it is of great importance in developing a new class of material with inherent bactericidal activity and good biocompatibility. In the present study, we have modified the aluminium surfaces by a coating of graphene oxide (GO) due to its excellent physicochemical properties, water dispersity and low cytotoxicity. Coatings were developed through facile and enviornment-friendly transfer method. The antimicrobial properties of GO coated aluminium are investigated against Gram-negative strain E. coli through agar plate counting and ‘Live/Dead’ fluorescence staining. Further, to shed light into the mechanism of antibacterial activity of GO at the molecular level, we have performed X-ray reflectivity (XRR) study considering a phospholipid multilayer as a model system to mimic bacteria cell membrane. Results show a significant bactericidal activity of the GO coatings compared to uncoated aluminium with lower concentration showing slightly better antibacterial property due to higher roughness. The obtained results may pave the way for engineering graphene-based antimicrobial coatings on a material surface using an easy, environment-friendly, cost-effective and straight forward processing route.
    Keywords Escherichia coli ; X-ray reflectivity ; agar ; aluminum ; antibacterial properties ; biocompatibility ; cell membranes ; cost effectiveness ; cytotoxicity ; environmental technology ; fluorescence ; graphene ; graphene oxide ; health services ; phospholipids
    Language English
    Dates of publication 2022-11
    Publishing place Elsevier B.V.
    Document type Article
    ISSN 2352-1864
    DOI 10.1016/j.eti.2022.102591
    Database NAL-Catalogue (AGRICOLA)

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  5. Article ; Online: Variations in the morphology of stylomastoid foramen: a possible solution to the conundrum of unexplained cases of Bell's palsy.

    Ghosh, S K / Narayan, R K

    Folia morphologica

    2020  Volume 80, Issue 1, Page(s) 97–105

    Abstract: Background: Stylomastoid foramen is the terminal part of facial canal and is the exit gateway for facial nerve from skull base. We hypothesized that anatomical variations of this foramen could be a risk factor for the injury of facial nerve resulting in ...

    Abstract Background: Stylomastoid foramen is the terminal part of facial canal and is the exit gateway for facial nerve from skull base. We hypothesized that anatomical variations of this foramen could be a risk factor for the injury of facial nerve resulting in unilateral facial nerve paralysis or Bell's palsy. Hence the present study was conducted to study the variations in size and shape of stylomastoid foramen in dry adult human skulls.
    Materials and methods: The study was conducted on 37 dry adult human skulls of unknown age and sex. High resolution images of the skulls under study were processed by ImageJ software and observations were undertaken.
    Results: Total eight variations of stylomastoid foramen were observed in terms of shape. The common variants were round, oval and square (present in 83.79% skulls on right side and 81.07% skulls on left side), whereas the rare variants were triangular, rectangular, serrated, bean-shaped and irregular. It was noted that stylomastoid foramen were associated with extensions (45.95% skulls) and also adjacent foramen (18.92% skulls). Exclusively unilateral observations included bifurcation of foramen (16.22% skulls), foramen situated deep inside skull groove (5.41% skulls) and foramen interrupted by bony spur (2.7% skulls). No significant differences were observed between the mean diameters (antero-posterior and transverse) of the stylomastoid foramen.
    Conclusions: The unilateral variations along with rare variations in terms of shape such as serrated, bean-shaped and irregular foramen (which were also unilateral findings) could be potential risk factors towards injury of facial nerve at the point of exit from skull base leading to Bell's palsy.
    MeSH term(s) Adult ; Bell Palsy ; Facial Nerve ; Facial Paralysis ; Humans ; Risk Factors ; Temporal Bone
    Language English
    Publishing date 2020-02-19
    Publishing country Poland
    Document type Journal Article
    ZDB-ID 419361-1
    ISSN 1644-3284 ; 0015-5659
    ISSN (online) 1644-3284
    ISSN 0015-5659
    DOI 10.5603/FM.a2020.0019
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Antiproliferative and apoptotic effect of ethanolic extract of Calocybe indica on PANC-1 and MIAPaCa2 cell lines of pancreatic cancer.

    Ghosh, S K / Sanyal, T

    Experimental oncology

    2020  Volume 42, Issue 3, Page(s) 178–182

    Abstract: Background: Pancreatic cancer is an aggressive malignancy with poor prognosis. New options for its treatment including the use of the substances of natural origin are now on the agenda.: Aim: An attempt has been taken to examine the effect of ... ...

    Abstract Background: Pancreatic cancer is an aggressive malignancy with poor prognosis. New options for its treatment including the use of the substances of natural origin are now on the agenda.
    Aim: An attempt has been taken to examine the effect of ethanolic extract (EE) of edible mushroom Calocybe indica on PANC-1 and MIAPaCa2 cell lines of pancreatic cancer in vitro.
    Materials and methods: Study of cell morphology, evaluation of apoptosis by DAPI staining under florescence microscope, anti-proliferative study by MTT assay, lactate dehydrogenase assay, pro-apoptotic and anti-apoptotic protein detection by Western blotting were performed to test effects of Calocybe indica EE on the cells of these two lines. Effect on in vitro migration was studied by scratch method.
    Results: Both cell lines treated with 250 or 500 µg/mL of EE underwent morphological changes - became more round and shrunken, with membrane blebbing and the reduced cell confluence. The nuclei of treated cells became condensed and fragmented. The percentage of apoptotic cells increased as EE concentration increased from 100 µg/mL to 500 µg/mL. Anti-proliferative effect was recorded at all concentrations within 100-750 µg/mL. The effect EE on lactate dehydrogenase leakage was concentration-dependent in both cell lines. Western blotting showed that caspase-3 and -9, and p53 protein levels were increased and BcL2 protein was decreased after treatment of the cells with EE compared to the control. PANC-1 cell migration was inhibited by 80.12 ± 4.25% after 24 h treatment of the cells with 500 µg/mL EE compared to the control.
    Conclusions: EE of Calocybe indica inhibits the growth and induces apoptosis in pancreatic cancer cell lines in vitro.
    Language English
    Publishing date 2020-09-30
    Publishing country Ukraine
    Document type Journal Article
    ZDB-ID 2176927-8
    ISSN 1812-9269 ; 0204-3564
    ISSN 1812-9269 ; 0204-3564
    DOI 10.32471/exp-oncology.2312-8852.vol-42-no-3.15086
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Anatomy of nervous system and emergence of neuroscience: A chronological journey across centuries.

    Ghosh, S K / Narayan, R K

    Morphologie : bulletin de l'Association des anatomistes

    2020  Volume 104, Issue 347, Page(s) 267–279

    Abstract: Scholars began exploring anatomy of nervous system from ancient times; however, considerable progress could only be made during the European Renaissance from the ... ...

    Abstract Scholars began exploring anatomy of nervous system from ancient times; however, considerable progress could only be made during the European Renaissance from the 14
    MeSH term(s) Anatomy ; Brain ; Dissection ; Nervous System ; Neurons ; Staining and Labeling
    Language English
    Publishing date 2020-06-10
    Publishing country France
    Document type Journal Article
    ZDB-ID 1443319-9
    ISSN 1286-0115
    ISSN 1286-0115
    DOI 10.1016/j.morpho.2020.05.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Histological and Ultrastructural Studies on the Intestine of Guntea Loach, Lepidocephalichthys Guntea (Cypriniformes, Cobitidae)

    Ghosh, S. K

    Vestnik zoologii. 2019 Apr. 01, v. 53, no. 2

    2019  

    Abstract: The cellular organizations of intestine in Lepidocephalichthys guntea (Hamilton, 1822) have been described by light as well as scanning and transmission electron microscopy. The intestine is short and straight like, marked into anterior, middle and ... ...

    Abstract The cellular organizations of intestine in Lepidocephalichthys guntea (Hamilton, 1822) have been described by light as well as scanning and transmission electron microscopy. The intestine is short and straight like, marked into anterior, middle and posterior region based on mucosal folds, number and size of columnar epithelial cells and mucous cells, thickness of submucosa and muscularis layer. The mucosa of anterior intestine forms high folds, which are lined with compactly arranged columnar epithelial cells and mucous cells. In the middle intestine, folds are pointless whereas the posterior intestine is without folds. The submucosa is formed of thin layer of connective tissue, contained collagen bundles and blood capillaries, comparatively well developed in the posterior intestine. By scanning electron microscopy, outlines of the luminal surface of anterior and middle intestine is embossed with oval or rounded columnar epithelial cells contained densely packed stubby microridges. The posterior intestine has closely set longitudinal folds characterized with minute blood capillaries and columnar epithelial cells having inconspicuous microridges. Ultrastructurally, the mucosal surface of the intestine consists of mucous cells with electron dense granules and columnar epithelial cells having numerous microvilli, mitochondria, endoplasmic reticulum, lysosomes and Golgi body. Cellular components of the anterior and middle intestine participate in the absorption whereas the presence of enormous blood vessels and capillary net work of posterior intestine probably responsible for air breathing.
    Keywords absorption ; air ; blood capillaries ; breathing ; collagen ; endoplasmic reticulum ; epithelial cells ; Golgi apparatus ; granules ; histology ; intestines ; Lepidocephalichthys guntea ; lysosomes ; microvilli ; mitochondria ; mucosa ; scanning electron microscopy ; transmission electron microscopy
    Language English
    Dates of publication 2019-0401
    Size p. 165-172.
    Publishing place Sciendo
    Document type Article
    ZDB-ID 2503821-7
    ISSN 2073-2333 ; 0084-5604
    ISSN (online) 2073-2333
    ISSN 0084-5604
    DOI 10.2478/vzoo-2019-0017
    Database NAL-Catalogue (AGRICOLA)

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  9. Article: Subcutaneous filariasis in India--Possible indication of human Dirofilariasis.

    Ghosh, S K

    Journal of vector borne diseases

    2016  Volume 53, Issue 1, Page(s) 90

    MeSH term(s) Cheek/pathology ; Edema/pathology ; Female ; Filariasis/diagnosis ; Filariasis/pathology ; Humans
    Language English
    Publishing date 2016-03
    Publishing country India
    Document type Comment ; Letter
    ZDB-ID 2161218-3
    ISSN 0972-9062
    ISSN 0972-9062
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Book ; Online: ENVIRONMENTAL SUSTAINABILITY ASSESSMENT OF A HIMALAYAN CATCHMENT WITH LAND COVER INDICES AND LST RELATIONSHIP USING PRINCIPAL COMPONENT ANALYSIS – A GEOSPATIAL APPROACH

    Sathyaseelan, M. / Ghosh, S. K. / Ojha, C. S. P.

    eISSN: 2194-9034

    2023  

    Abstract: Environmental sustainability assessment is a crucial part of the management of natural resources. Remote Sensing based environmental land cover indices such as Normalized Difference Vegetation Index (NDVI), Normalized Difference Water Index (NDWI), ... ...

    Abstract Environmental sustainability assessment is a crucial part of the management of natural resources. Remote Sensing based environmental land cover indices such as Normalized Difference Vegetation Index (NDVI), Normalized Difference Water Index (NDWI), Normalized Difference Built-up Index (NDBI), Normalized Difference Moisture Index (NDMI), and its associated Land Surface Temperature (LST) are the major governing factors for the environmental processes that happen on the surface of the earth . These NDVI, NDWI, NDBI, NDMI, and LST are generated for 2020 using the Landsat satellite datasets. The process-based relationship among them is complex and involves various parameters but may be easily represented by multiple linear regression models. Principal Component Analysis (PCA) is one such type that efficiently handles and evaluates the contribution of each of these factors to each other based on the sampling units. The study area is the upper Ramganga catchment in the Indian Himalayas, consisting of 117 sub-catchments. These catchment units (samples) are entangled with these environmental factors. The results of the PCA reveal the relationship between each of the environmental factors and their priority. Based on the uncorrelated factors priority suggestion from the PCA, catchment units were classified as high, moderate, or low categories based on their dominance in the relationship among the factors. These spatial variations in the environmental factors can help to assess the sustainability of resources in the Himalayan catchment.
    Subject code 333
    Language English
    Publishing date 2023-04-21
    Publishing country de
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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