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  1. Book: Basophils and mast cells

    Gibbs, Bernhard F. / Falcone, Franco Harald

    methods and protocols

    (Methods in molecular biology ; 2163 ; Springer protocols)

    2020  

    Author's details edited by Bernhard F. Gibbs, Franco H. Falcone
    Series title Methods in molecular biology ; 2163
    Springer protocols
    Collection
    Language English
    Size xviii, 380 Seiten, Illustrationen, 26 cm
    Edition Second edition
    Publisher Humana Press
    Publishing place New York, NY
    Publishing country United States
    Document type Book
    HBZ-ID HT020558175
    ISBN 978-1-0716-0695-7 ; 9781071606964 ; 1-0716-0695-6 ; 1071606964
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Zs A 7042 -2163- verfügbar in Königswinter Königswinter

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  2. Book: Basophils and mast cells

    Gibbs, Bernhard F. / Falcone, Franco H.

    methods and protocols

    (Methods in molecular biology ; 1192 ; Springer protocols)

    2014  

    Author's details ed. by Bernhard F. Gibbs ; Franco H. Falcone
    Series title Methods in molecular biology ; 1192
    Springer protocols
    Collection
    Language English
    Size XI, 251 S. : Ill., graph. Darst., 26 cm
    Publisher Humana Press
    Publishing place New York u.a.
    Publishing country United States
    Document type Book
    Note Includes bibliographical references and index
    HBZ-ID HT018487162
    ISBN 978-1-4939-1172-1 ; 1-4939-1172-4 ; 1493911732 ; 9781493911738
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Zs A 7042 -1192- verfügbar in Königswinter Königswinter

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  3. Article ; Online: IgE-dependent human basophil responses are inversely associated with the sarcoplasmic reticulum Ca

    Selnø, Anette T Hansen / Sumbayev, Vadim V / Gibbs, Bernhard F

    Frontiers in immunology

    2023  Volume 13, Page(s) 1052290

    Abstract: Basophils crucially contribute to allergies and other Th2-driven diseases by rapidly releasing inflammatory and immunomodulatory mediators following high-affinity IgE-receptor crosslinking. Although these basophil-mediated responses depend on ... ...

    Abstract Basophils crucially contribute to allergies and other Th2-driven diseases by rapidly releasing inflammatory and immunomodulatory mediators following high-affinity IgE-receptor crosslinking. Although these basophil-mediated responses depend on sensitization with antigen-specific IgE, this does not necessarily predict clinical symptom severity. It is thought that the balance of early stimulatory (e.g. SYK) and inhibitory (e.g. SHIP-1) intracellular signals are associated with basophil responsiveness, which is also critically dependent on calcium mobilization. Previous studies suggest that the sarcoplasmic reticulum Ca
    MeSH term(s) Humans ; Basophils/metabolism ; Immunoglobulin E/metabolism ; Calcium/metabolism ; Sarcoplasmic Reticulum Calcium-Transporting ATPases/metabolism ; Sarcoplasmic Reticulum Calcium-Transporting ATPases/pharmacology ; Asthma/metabolism
    Chemical Substances Immunoglobulin E (37341-29-0) ; Calcium (SY7Q814VUP) ; Sarcoplasmic Reticulum Calcium-Transporting ATPases (EC 3.6.3.8)
    Language English
    Publishing date 2023-01-06
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2022.1052290
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Editorial: Pathological reactions of cytotoxic lymphoid cells as universal therapeutic targets in cancer and autoimmune disease.

    Sumbayev, Vadim V / Gibbs, Bernhard F / Fasler-Kan, Elizaveta

    Frontiers in medicine

    2023  Volume 10, Page(s) 1186318

    Language English
    Publishing date 2023-04-25
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2775999-4
    ISSN 2296-858X
    ISSN 2296-858X
    DOI 10.3389/fmed.2023.1186318
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: "Novel insights into the roles of mast cells and basophils": Joint Webinar of the Japanese and the European Histamine Research Societies (JHRS/EHRS).

    Tiligada, Ekaterini / Gibbs, Bernhard F / Tanaka, Satoshi

    Inflammation research : official journal of the European Histamine Research Society ... [et al.

    2022  Volume 71, Issue 7-8, Page(s) 991–993

    Abstract: The joint webinar of the Japanese (JHRS) and the European (EHRS) Histamine Research Society focusing on "Novel insights into the roles of mast cells and basophils" was organized in hybrid format on January 7, 2022 during the 23rd meeting of the JHRS held ...

    Abstract The joint webinar of the Japanese (JHRS) and the European (EHRS) Histamine Research Society focusing on "Novel insights into the roles of mast cells and basophils" was organized in hybrid format on January 7, 2022 during the 23rd meeting of the JHRS held in Kyoto, Japan. Tissue mast cells and circulating basophils are the primary sources of histamine, and they are considered to be pivotal components shaping inflammatory and immune-related processes. The webinar comprised four lectures delivered by experts in the field from Japan and the European Mast Cell and Basophil Research Network (EMBRN) that exposed novel insights into the contribution of basophils and mast cells in inflammatory and (auto)immune diseases, including allergies, asthma, and urticaria. Several targets were also highlighted in terms of developing novel and improved treatments for these pathologies.
    MeSH term(s) Basophils ; Histamine ; Histamine Release ; Japan ; Mast Cells
    Chemical Substances Histamine (820484N8I3)
    Language English
    Publishing date 2022-05-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1221794-3
    ISSN 1420-908X ; 1023-3830
    ISSN (online) 1420-908X
    ISSN 1023-3830
    DOI 10.1007/s00011-022-01575-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 675: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  6. Article ; Online: Gene Silencing Approaches in Mast Cells and Primary Human Basophils.

    Sumbayev, Vadim V / Gibbs, Bernhard F

    Methods in molecular biology (Clifton, N.J.)

    2020  Volume 2163, Page(s) 323–330

    Abstract: The ability to silence gene expression is an invaluable tool for elucidating the importance of intracellular signaling proteins which contribute to the effector functions of mast cells and basophils. However, primary mast cells and their terminally ... ...

    Abstract The ability to silence gene expression is an invaluable tool for elucidating the importance of intracellular signaling proteins which contribute to the effector functions of mast cells and basophils. However, primary mast cells and their terminally differentiated blood counterpart, basophils, pose a difficult challenge for gene silencing approaches given not only their state of maturation and difficulty to transfect but also because their functions are readily altered by cell handling conditions. Here, we describe a method using lipofection which has been successfully employed to silence gene expression using siRNA in human LAD2 mast cells as well as primary human basophils.
    MeSH term(s) Basophils/chemistry ; Basophils/cytology ; Basophils/metabolism ; Cells, Cultured ; Fatty Acids, Monounsaturated/chemistry ; Fatty Acids, Monounsaturated/metabolism ; Gene Silencing ; Humans ; Liposomes/chemistry ; Liposomes/metabolism ; Mast Cells/chemistry ; Mast Cells/cytology ; Mast Cells/metabolism ; Primary Cell Culture/methods ; Quaternary Ammonium Compounds/chemistry ; Quaternary Ammonium Compounds/metabolism ; RNA, Small Interfering/genetics ; RNA, Small Interfering/metabolism ; Transfection/methods
    Chemical Substances Fatty Acids, Monounsaturated ; Liposomes ; Quaternary Ammonium Compounds ; RNA, Small Interfering ; 1,2-dioleoyloxy-3-(trimethylammonium)propane (MR86K0XRQP)
    Language English
    Publishing date 2020-08-05
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-0696-4_26
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Basophil Stimulation and Signaling Pathways.

    Knol, Edward F / Gibbs, Bernhard F

    Methods in molecular biology (Clifton, N.J.)

    2020  Volume 2163, Page(s) 311–322

    Abstract: Despite the growing use of flow cytometry to analyze the functional characteristics of basophils, the intracellular signaling cascades that control their ability to elaborate various pro-allergic and inflammatory mediators and cytokines remain ... ...

    Abstract Despite the growing use of flow cytometry to analyze the functional characteristics of basophils, the intracellular signaling cascades that control their ability to elaborate various pro-allergic and inflammatory mediators and cytokines remain comparatively obscure. Additionally, some studies require the analysis of pro-allergic and inflammatory mediators, such as histamine, LTC
    MeSH term(s) Basophils/metabolism ; Blotting, Western ; Calcium/analysis ; Calcium/metabolism ; Cell Degranulation/physiology ; Cells, Cultured ; Cytokines/analysis ; Cytokines/metabolism ; Eicosanoids/analysis ; Eicosanoids/metabolism ; Enzyme-Linked Immunosorbent Assay ; Flow Cytometry/methods ; Histamine/analysis ; Histamine/metabolism ; Humans ; Intracellular Signaling Peptides and Proteins/metabolism ; Signal Transduction ; Spectrometry, Fluorescence/methods ; Staining and Labeling/methods
    Chemical Substances Cytokines ; Eicosanoids ; Intracellular Signaling Peptides and Proteins ; Histamine (820484N8I3) ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2020-08-05
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-0696-4_25
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Purification of Basophils from Peripheral Human Blood.

    Falcone, Franco H / Gibbs, Bernhard F

    Methods in molecular biology (Clifton, N.J.)

    2020  Volume 2163, Page(s) 35–48

    Abstract: The purification of basophils from peripheral blood has represented a formidable challenge for researchers since they were discovered by Paul Ehrlich in 1879. From the first published attempts in the late 1960s, it took half a century to develop robust ... ...

    Abstract The purification of basophils from peripheral blood has represented a formidable challenge for researchers since they were discovered by Paul Ehrlich in 1879. From the first published attempts in the late 1960s, it took half a century to develop robust protocols able to give sufficient numbers of pure, functionally unimpaired basophils. The existing protocols for basophil purification exploit those properties of basophils which distinguish them from other cell types such as their localization in blood, density, and the presence or absence of surface markers. Purification techniques have been used in various combinations and variations to achieve a common goal in mind: to obtain a pure population of human basophils in sufficient numbers for downstream studies. The arduous way leading up to the modern protocols is summarized in this historical retrospective. A fast protocol for purification of basophils to near homogeneity is also described.
    MeSH term(s) Basophils/classification ; Basophils/cytology ; Basophils/metabolism ; Cell Separation/methods ; Cells, Cultured ; Humans ; Primary Cell Culture/methods
    Language English
    Publishing date 2020-08-05
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-0696-4_2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Basophil Stimulation and Signaling Pathways

    Knol, Edward F / Gibbs, Bernhard F

    Methods Mol Biol

    Abstract: Despite the growing use of flow cytometry to analyze the functional characteristics of basophils, the intracellular signaling cascades that control their ability to elaborate various pro-allergic and inflammatory mediators and cytokines remain ... ...

    Abstract Despite the growing use of flow cytometry to analyze the functional characteristics of basophils, the intracellular signaling cascades that control their ability to elaborate various pro-allergic and inflammatory mediators and cytokines remain comparatively obscure. Additionally, some studies require the analysis of pro-allergic and inflammatory mediators, such as histamine, LTC4, and various basophil-derived cytokines (e.g., IL-4 and IL-13). Elucidation of intracellular signaling proteins by Western blotting, cytosolic free calcium concentration by spectrofluorophotometry, and detection of mediator releases, as well as analysis of gene expressions by RT-PCR, generally requires relatively large numbers of purified basophils. In selected assays, flow cytometry enables the analysis of relatively low cell numbers and purity for the expression of intracellular signaling proteins or measurement of cytosolic free calcium concentrations by basophil-specific gating strategies. Unfortunately, many aspects of signal transduction relevant to human basophils cannot be readily extrapolated from the use of basophil or mast cell lines. This chapter therefore focuses on how to employ primary human basophils for studying mediator releases and signaling characteristics.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #32766986
    Database COVID19

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  10. Article: Gene Silencing Approaches in Mast Cells and Primary Human Basophils

    Sumbayev, Vadim V / Gibbs, Bernhard F

    Methods Mol Biol

    Abstract: The ability to silence gene expression is an invaluable tool for elucidating the importance of intracellular signaling proteins which contribute to the effector functions of mast cells and basophils. However, primary mast cells and their terminally ... ...

    Abstract The ability to silence gene expression is an invaluable tool for elucidating the importance of intracellular signaling proteins which contribute to the effector functions of mast cells and basophils. However, primary mast cells and their terminally differentiated blood counterpart, basophils, pose a difficult challenge for gene silencing approaches given not only their state of maturation and difficulty to transfect but also because their functions are readily altered by cell handling conditions. Here, we describe a method using lipofection which has been successfully employed to silence gene expression using siRNA in human LAD2 mast cells as well as primary human basophils.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #32766987
    Database COVID19

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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