Article ; Online: Acclimation Prior to an Intraperitoneal Insulin Tolerance Test to Mitigate Stress-Induced Hyperglycemia in Conscious Mice.
Journal of visualized experiments : JoVE
2020 , Issue 159
Abstract: The insulin tolerance test is commonly used in metabolic studies to assess whole body insulin sensitivity in rodents. It is a relatively simple test that involves measurement of blood glucose levels over time following a single intraperitoneal injection ... ...
| Abstract | The insulin tolerance test is commonly used in metabolic studies to assess whole body insulin sensitivity in rodents. It is a relatively simple test that involves measurement of blood glucose levels over time following a single intraperitoneal injection of insulin. Given that it is performed in the conscious state and blood is often collected via a tail snip, it has the potential to elicit a stress response from animals due to anxiety associated with handling and blood collection. As such, a stress-induced rise in blood glucose can occur, making it difficult to detect and interpret the primary endpoint measure, namely an insulin-mediated reduction in blood glucose. This has been seen in many mouse strains, and is quite common in diabetic db/db mice, where glucose levels can increase, rather than decrease, after insulin administration. Here, we describe a method of acclimating mice to handling, injections and blood sampling prior to performing the insulin tolerance test. We find that this lowers stress-induced hyperglycemia and results in data that more accurately reflects whole body insulin sensitivity. |
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| MeSH term(s) | Acclimatization ; Animals ; Artifacts ; Blood Glucose/metabolism ; Hyperglycemia/blood ; Hyperglycemia/complications ; Hyperglycemia/metabolism ; Hyperglycemia/psychology ; Insulin Resistance ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Inbred Strains ; Stress, Psychological/complications ; Stress, Psychological/physiopathology |
| Chemical Substances | Blood Glucose |
| Language | English |
| Publishing date | 2020-05-22 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Video-Audio Media |
| ZDB-ID | 2259946-0 |
| ISSN | 1940-087X ; 1940-087X |
| ISSN (online) | 1940-087X |
| ISSN | 1940-087X |
| DOI | 10.3791/61179 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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