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  1. Article: A psychiatry training program for family physicians.

    Gilbert, J R

    Canadian family physician Medecin de famille canadien

    2010  Volume 17, Issue 3, Page(s) 61–62

    Abstract: This is an overview of an ongoing project by the Departments of Family Medicine and Psychiatry at McMaster University to develop a model of psychotherapy for family physicians. ...

    Abstract This is an overview of an ongoing project by the Departments of Family Medicine and Psychiatry at McMaster University to develop a model of psychotherapy for family physicians.
    Language English
    Publishing date 2010-05-01
    Publishing country Canada
    Document type Journal Article
    ZDB-ID 603565-6
    ISSN 0008-350X
    ISSN 0008-350X
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Management of low-back pain in family practice: a critical review.

    Gilbert, J R

    Canadian family physician Medecin de famille canadien

    2010  Volume 32, Page(s) 1855–1861

    Abstract: There is a profusion of both orthodox and unorthodox treatments for low-back pain, many of which have been inadequately evaluated. Conflicting claims exist for nearly all of these treatments. To assess the evidence supporting these commonly used ... ...

    Abstract There is a profusion of both orthodox and unorthodox treatments for low-back pain, many of which have been inadequately evaluated. Conflicting claims exist for nearly all of these treatments. To assess the evidence supporting these commonly used conservative therapies in family practice, a set of methodological criteria for evaluating the validity and usefulness of the results was applied to original articles which described trials of bed rest, exercises, manipulation, drug therapy, advice/education, and other therapies such as traction, corsets and transcutaneous nerve stimulation (TNS). Guidelines for managing acute back pain and acute back pain with sciatica are indicated. Key questions and certain physical signs which suggest a functional overlay in a patient with chronic back pain are also outlined.
    Language English
    Publishing date 2010-12-29
    Publishing country Canada
    Document type Journal Article
    ZDB-ID 603565-6
    ISSN 0008-350X
    ISSN 0008-350X
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Career characteristics of canadian family medicine residents.

    Gilbert, J R / Norman, G R

    Canadian family physician Medecin de famille canadien

    2010  Volume 21, Issue 2, Page(s) 151–161

    Abstract: A questionnaire was distributed to first-year residents entering all family practice programs in Canada for the years 1966-1973. The content of the questionnaire elicited information on a number of demographic and career choice variables.The analysis of ... ...

    Abstract A questionnaire was distributed to first-year residents entering all family practice programs in Canada for the years 1966-1973. The content of the questionnaire elicited information on a number of demographic and career choice variables.The analysis of career choice data indicated a trend toward salaried or institutional employment in comparison with similar data from medical students. Full-time involvement in teaching or research is low; however, the majority of residents indicated a desire to pursue both as a minor part of their career.
    Language English
    Publishing date 2010-05-01
    Publishing country Canada
    Document type Journal Article
    ZDB-ID 603565-6
    ISSN 0008-350X
    ISSN 0008-350X
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Predicting outcome in acute low-back pain.

    Singer, J / Gilbert, J R / Hutton, T / Taylor, D W

    Canadian family physician Medecin de famille canadien

    2010  Volume 33, Page(s) 655–659

    Abstract: Patients presenting to their family physician with acute low-back pain were studied prospectively. Demographic factors and patient history at the initial visit were assessed to determine important predictors of selected clinical outcomes, including time ... ...

    Abstract Patients presenting to their family physician with acute low-back pain were studied prospectively. Demographic factors and patient history at the initial visit were assessed to determine important predictors of selected clinical outcomes, including time to resumption of normal activities and time to relief from pain. While several predictors were significantly correlated with each of the outcomes assessed, the most consistent predictor of outcome proved to be the reported pain intensity at the initial visit. Baseline levels of pain intensity were related to expected time of recovery and probability of periodic pain in the future. Data collected in the initial history and physical examination of patients permit an assessment of factors that may be useful in establishing prognosis for relevant clinical outcomes.
    Language English
    Publishing date 2010-12-16
    Publishing country Canada
    Document type Journal Article
    ZDB-ID 603565-6
    ISSN 0008-350X
    ISSN 0008-350X
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Isolation of genomic DNA from mammalian cells.

    Gilbert, J R / Vance, J M

    Current protocols in human genetics

    2001  Volume Appendix 3, Page(s) Appendix 3B

    Abstract: This unit describes simple, cost-effective preparation of DNA from whole blood or cultured cells that yields high-molecular-weight DNA suitable for both Southern blotting and the polymerase chain reaction. Preparation time may be shortened by ... ...

    Abstract This unit describes simple, cost-effective preparation of DNA from whole blood or cultured cells that yields high-molecular-weight DNA suitable for both Southern blotting and the polymerase chain reaction. Preparation time may be shortened by substituting a high-salt precipitation procedure for the dialysis step; however, this results in a smaller average fragment size. The isolation of DNA from buccal swabs, collected from the inside of the cheek, is also described. The DNA is suitable for PCR analysis. Preparation of buffered phenol for DNA extraction is described in a support protocol. This unit describes simple, cost-effective preparation of DNA from whole blood or cultured cells that yields high-molecular-we.
    MeSH term(s) Cells, Cultured ; DNA/blood ; DNA/genetics ; DNA/isolation & purification ; Genetic Techniques ; Genetics, Medical ; Humans ; Mouth Mucosa/chemistry
    Chemical Substances DNA (9007-49-2)
    Language English
    Publishing date 2001-05
    Publishing country United States
    Document type Journal Article
    ISSN 1934-8258
    ISSN (online) 1934-8258
    DOI 10.1002/0471142905.hga03bs19
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: HIV-2 and SIV vector systems.

    Gilbert, J R / Wong-Staal, F

    Somatic cell and molecular genetics

    2003  Volume 26, Issue 1-6, Page(s) 83–98

    Abstract: Lentiviral vectors have received much attention in recent years due to their ability to efficiently transduce non-dividing cells. Of the lentiviruses HIV-2 and SIV offer several unique benefits as the basis for lentiviral vector design. HIV-1, HIV-2 and ... ...

    Abstract Lentiviral vectors have received much attention in recent years due to their ability to efficiently transduce non-dividing cells. Of the lentiviruses HIV-2 and SIV offer several unique benefits as the basis for lentiviral vector design. HIV-1, HIV-2 and SIV remain the only known primate lentiviruses, and consequently are among the most extensively studied viruses known. Substantial effort has been devoted towards identifying the pathogenic determinants of the primate lentiviruses and towards understanding their replication within primates. Of the primate lentiviruses, the pathogenicity and rates of transmission of HIV-2 and SIV fall far below that of HIV-1, potentially providing vectors based upon HIV-2/SIV with a greater degree of biosafety. Last, and perhaps most importantly, HIV-2 and SIV are viruses which may be studied within non-human primate models susceptible to AIDS-like disease, making vectors based upon these viruses accessible to substantial preclinical evaluation. We approach this Chapter presenting information regarding the basic biology of HIV-2 and SIV and conclude by pointing to how unique features of HIV-2 and SIV are well suited to vector design, hoping to leave the reader with a greater appreciation of the potential these viruses offer within the field of gene transfer applications.
    MeSH term(s) Animals ; Gene Expression Regulation, Viral ; Gene Transfer Techniques ; Genetic Vectors/genetics ; Genome, Viral ; HIV-2/genetics ; Humans ; Lentivirus/genetics ; Primates ; Simian Immunodeficiency Virus/genetics
    Language English
    Publishing date 2003-04-01
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 605729-9
    ISSN 1572-9931 ; 0740-7750
    ISSN (online) 1572-9931
    ISSN 0740-7750
    DOI 10.1023/a:1021026730034
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Exome Sequencing of Extended Families with Alzheimer's Disease Identifies Novel Genes Implicated in Cell Immunity and Neuronal Function.

    Cukier, H N / Kunkle, B K / Hamilton, K L / Rolati, S / Kohli, M A / Whitehead, P L / Jaworski, J / Vance, J M / Cuccaro, M L / Carney, R M / Gilbert, J R / Farrer, L A / Martin, E R / Beecham, G W / Haines, J L / Pericak-Vance, M A

    Journal of Alzheimer's disease & Parkinsonism

    2017  Volume 7, Issue 4

    Abstract: Objective: Alzheimer's disease (AD) is a neurodegenerative disorder for which more than 20 genetic loci have been implicated to date. However, studies demonstrate not all genetic factors have been identified. Therefore, in this study we seek to identify ...

    Abstract Objective: Alzheimer's disease (AD) is a neurodegenerative disorder for which more than 20 genetic loci have been implicated to date. However, studies demonstrate not all genetic factors have been identified. Therefore, in this study we seek to identify additional rare variants and novel genes potentially contributing to AD.
    Methods: Whole exome sequencing was performed on 23 multi-generational families with an average of eight affected subjects. Exome sequencing was filtered for rare, nonsynonymous and loss-of-function variants. Alterations predicted to have a functional consequence and located within either a previously reported AD gene, a linkage peak (LOD>2), or clustering in the same gene across multiple families, were prioritized.
    Results: Rare variants were found in known AD risk genes including
    Conclusion: Utilizing large families with a heavy burden of disease allowed for the identification of rare variants co-segregating with disease. Variants were identified in both known AD risk genes and in novel genes.
    Language English
    Publishing date 2017-07-31
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2711981-6
    ISSN 2161-0460
    ISSN 2161-0460
    DOI 10.4172/2161-0460.1000355
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Targeted sequencing of ABCA7 identifies splicing, stop-gain and intronic risk variants for Alzheimer disease.

    Kunkle, B W / Carney, R M / Kohli, M A / Naj, A C / Hamilton-Nelson, K L / Whitehead, P L / Wang, L / Lang, R / Cuccaro, M L / Vance, J M / Byrd, G S / Beecham, G W / Gilbert, J R / Martin, E R / Haines, J L / Pericak-Vance, M A

    Neuroscience letters

    2017  Volume 649, Page(s) 124–129

    Abstract: Several variants in the gene ABCA7 have been identified as potential causal variants for late-onset Alzheimer's disease (LOAD). In order to replicate these findings, and search for novel causal variants, we performed targeted sequencing of this gene in ... ...

    Abstract Several variants in the gene ABCA7 have been identified as potential causal variants for late-onset Alzheimer's disease (LOAD). In order to replicate these findings, and search for novel causal variants, we performed targeted sequencing of this gene in cohorts of non-Hispanic White (NHW) and African-American (AA) LOAD cases and controls. We sequenced the gene ABCA7 in 291 NHW LOAD cases and 103 controls. Variants were prioritized for rare, damaging variants and previously reported variants associated with LOAD, and were follow-up genotyped in 4076 NHW and 1157 AA cases and controls. We confirm three previously associated ABCA7 risk variants and extend two of these associations to other populations, an intronic variant in NHW (P=3.0×10
    MeSH term(s) ATP-Binding Cassette Transporters/genetics ; African Americans/genetics ; Alzheimer Disease/genetics ; European Continental Ancestry Group/genetics ; Female ; Genetic Association Studies ; Genetic Predisposition to Disease ; Genotype ; Humans ; Introns ; Male ; Pedigree ; Polymorphism, Single Nucleotide ; Sequence Analysis, DNA
    Chemical Substances ABCA7 protein, human ; ATP-Binding Cassette Transporters
    Language English
    Publishing date 2017-04-08
    Publishing country Ireland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 194929-9
    ISSN 1872-7972 ; 0304-3940
    ISSN (online) 1872-7972
    ISSN 0304-3940
    DOI 10.1016/j.neulet.2017.04.014
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: The genes encoding for D4Z4 binding proteins HMGB2, YY1, NCL, and MYOD1 are excluded as candidate genes for FSHD1B.

    Bastress, K L / Stajich, J M / Speer, M C / Gilbert, J R

    Neuromuscular disorders : NMD

    2005  Volume 15, Issue 4, Page(s) 316–320

    Abstract: Facioscapulohumeral muscular dystrophy is a disease of skeletal muscle, with symptoms including both facial and shoulder girdle weakness and progression to involve the pelvic girdle and extremities in the majority of cases. For most cases of FSHD, the ... ...

    Abstract Facioscapulohumeral muscular dystrophy is a disease of skeletal muscle, with symptoms including both facial and shoulder girdle weakness and progression to involve the pelvic girdle and extremities in the majority of cases. For most cases of FSHD, the molecular basis of the disease can be identified as a partial deletion of the D4Z4 repeat array on the end of the long arm of chromosome 4. However, in up to 5% of FSHD families there is no linkage to 4q35. These cases are designated as FSHD1B. Proteins have been identified that bind to the D4Z4 repeats of chromosome 4q35. The genes encoding D4Z4 binding proteins YY1, HMGB2, NCL, and MYOD1 were investigated as candidate genes for FSHD1B. Coding sequences and promoter region were analyzed for HMBG2 and no sequence variations were detected. For YY1, all five exons were analyzed and a polymorphism was detected in both the unaffected and affected populations. In nucleolin (NCL), several SNPs were identified, including a SNP causing the non-synonymous change P515H; however, all polymorphisms either occurred in control samples or were previously reported. A novel polymorphism was also detected in MYOD1, but did not represent a disease-specific variation. These results suggest that HMBG2, YY1, NCL, and MYOD1 are unlikely to represent the genes responsible for FSHD in these families.
    MeSH term(s) Chromatography, High Pressure Liquid/methods ; Chromosomes, Human, Pair 4 ; DNA Mutational Analysis/methods ; DNA-Binding Proteins/genetics ; Erythroid-Specific DNA-Binding Factors ; Exons ; Family Health ; HMGB2 Protein/genetics ; Humans ; Muscular Dystrophy, Facioscapulohumeral/genetics ; MyoD Protein/genetics ; Phosphoproteins/genetics ; Promoter Regions, Genetic ; RNA-Binding Proteins/genetics ; Repetitive Sequences, Nucleic Acid ; Transcription Factors/genetics ; YY1 Transcription Factor ; Nucleolin
    Chemical Substances DNA-Binding Proteins ; Erythroid-Specific DNA-Binding Factors ; HMGB2 Protein ; MyoD Protein ; MyoD1 myogenic differentiation protein ; Phosphoproteins ; RNA-Binding Proteins ; Transcription Factors ; YY1 Transcription Factor ; YY1 protein, human
    Language English
    Publishing date 2005-01-28
    Publishing country England
    Document type Comparative Study ; Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 1077681-3
    ISSN 1873-2364 ; 0960-8966
    ISSN (online) 1873-2364
    ISSN 0960-8966
    DOI 10.1016/j.nmd.2004.12.006
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: How many well baby visits? A randomized trial in progress.

    Gilbert, J R / Feldman, W / Mills, D A / Siegel, L / Dunnett, C W / Stoddart, G / Durcan, T

    Canadian family physician Medecin de famille canadien

    2011  Volume 26, Page(s) 1178–1181

    Abstract: The objective of this study in progress is to determine whether decreasing the number of well-baby visits during the first two years of life from the currently allowable ten visits to five, can be done with efficacious and safe results. Five hundred ... ...

    Abstract The objective of this study in progress is to determine whether decreasing the number of well-baby visits during the first two years of life from the currently allowable ten visits to five, can be done with efficacious and safe results. Five hundred babies under the care of their family physicians were randomly assigned to an experimental group receiving five well-baby visits in the first two years of life or to a control group receiving ten visits. This article presents the results pertaining to the collection of the cohort.
    Language English
    Publishing date 2011-02-04
    Publishing country Canada
    Document type Journal Article
    ZDB-ID 603565-6
    ISSN 0008-350X
    ISSN 0008-350X
    Database MEDical Literature Analysis and Retrieval System OnLINE

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