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  1. Article ; Online: Rhodomyrtus tomentosa Fruit Extract and Skin Microbiota

    Sandie Gervason / Isabelle Metton / Elodie Gemrot / Edwige Ranouille / Gilbert Skorski / Magalie Cabannes / Jean-Yves Berthon / Edith Filaire

    Cosmetics, Vol 7, Iss 53, p

    A Focus on C. acnes Phylotypes in Acne Subjects

    2020  Volume 53

    Abstract: Knowing that Rhodomyrtus tomentosa is known to have antibacterial effects, this study investigated the skin microbiota with a focus on Cutibacterium acnes ( C. acnes ) phylotypes in subjects with acne, and determined microbiota changes after 28 days of ... ...

    Abstract Knowing that Rhodomyrtus tomentosa is known to have antibacterial effects, this study investigated the skin microbiota with a focus on Cutibacterium acnes ( C. acnes ) phylotypes in subjects with acne, and determined microbiota changes after 28 days of treatment with berries Rhodomyrtus tomentosa as an active ingredient ( RT ). Skin swabs from seventeen acne subjects were collected and the skin microbiome was analyzed using 16S rRNA gene sequencing. A culture-independent next-generation sequencing (NGS)-based SLST (single-locus sequence typing) approach was aimed at evaluating RT extract effects on C. acnes phylotype repartition. Clinical evaluations (lesion counts) were performed at baseline (D0) and after 28 days (D28) of twice-daily application of the RT active ingredient. We determined: (1) the skin microbiota at D0 was dominated by Actinobacteria followed by Firmicutes and Proteobacteria; (2) at the genus level, Cutibacterium was the most abundant genus followed by Staphylococcus and Corynebacterium

    (3) C. acnes was the major species in terms of mean abundance, followed by Staphylococcus epidermidis ( S. epidermidis ) and Staphylococcus hominis ( S. hominis ); and (4) phylotype IA1 was most represented, with a predominance of SLST type A1, followed by phylotypes II, IB, IA2, IC, and III. After 28 days of RT extract treatment, phylotype repartition were modified with a decrease in abundance (approximately 4%) of phylotype IA1 and an increase in phylotype II and III. Cutibacterium granulosum ( C. granulosum ) abundance also decreased. Reduction of retentional and inflammatory lesions was also noted only after RT treatment; thus, RT extract acts as a microbiota-regulating agent.
    Keywords acne ; microbiota ; Cutibacterium acnes ; phylotype ; Rhodomyrtus tomentosa ; Chemistry ; QD1-999
    Subject code 616
    Language English
    Publishing date 2020-07-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article: Allergen immobilisation and signal amplification by quantum dots for use in a biosensor assay of IgE in serum

    Platt, Geoffrey W / Francesco Damin / Gilbert Skorski / Isabelle Metton / Marcella Chiari / Marcus J. Swann / Marina Cretich

    Biosensors & bioelectronics. 2014 Feb. 15, v. 52

    2014  

    Abstract: The production of biosensors for point of care diagnostics usually requires the immobilisation and storage of protein (for example, antigen or antibody) on a sensor surface, in a manner that retains a high degree of activity and low levels of non- ... ...

    Abstract The production of biosensors for point of care diagnostics usually requires the immobilisation and storage of protein (for example, antigen or antibody) on a sensor surface, in a manner that retains a high degree of activity and low levels of non-specific binding. These characteristics have been assessed for polymer immobilised antigens (allergens) using an IgG binding assay and demonstrated further by assay with serum containing reactive IgEs.The activity of allergens immobilised on sensor chips using copoly(DMA–NAS–MAPS) and a spotting technique, as well as the specificity of their binding interactions with cognate immunoglobulins was assessed using Dual Polarisation Interferometry (DPI). The data obtained indicate that the allergens studied remain stable over long periods of time (at least 114 days). This performance compared favourably with other immobilisation methods. Allergen coated chips were tested in an anti-casein IgE assay using human serum from allergic and non-allergic donors. Detection of both total Ig and specific IgE was demonstrated using a secondary anti-IgE antibody. Furthermore, optical signal enhancement with streptavidin conjugated quantum dots was shown to yield responses for samples below 0.84ng/mL (0.35KU/L) of IgE, which overlap with the industrial quasi-standard ImmunoCAP® and is the clinically relevant threshold used to classify serum samples from allergic individuals.
    Keywords allergens ; antibodies ; biosensors ; blood serum ; diagnostic techniques ; humans ; immunoglobulin E ; immunoglobulin G ; interferometry ; polymers ; quantum dots ; streptavidin
    Language English
    Dates of publication 2014-0215
    Size p. 82-88.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1011023-9
    ISSN 1873-4235 ; 0956-5663
    ISSN (online) 1873-4235
    ISSN 0956-5663
    DOI 10.1016/j.bios.2013.08.019
    Database NAL-Catalogue (AGRICOLA)

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