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  1. Article ; Online: Improvements of virulence factor phenotypic tests for Aeromonas salmonicida subsp. salmonicida, a major fish pathogen.

    Girard, Sarah B / Paquet, Valérie E / Charette, Steve J

    Journal of fish diseases

    2021  Volume 45, Issue 1, Page(s) 177–184

    Abstract: Aeromonas salmonicida subspecies salmonicida, a fish pathogen, expresses various virulence factors such as an A-layer, lipases and proteases during the infection process. Not all strains of this bacterium express the same virulence factors. It is ... ...

    Abstract Aeromonas salmonicida subspecies salmonicida, a fish pathogen, expresses various virulence factors such as an A-layer, lipases and proteases during the infection process. Not all strains of this bacterium express the same virulence factors. It is important to be able to evaluate which factors are present when characterizing strains. The A-layer and secreted lipases and proteases are usually detected by agar-based tests that require long incubation (24 h and more) and may provide ambiguous results. In the present study, protocols have been optimized to determine the presence of these virulence factors using liquid tests. For A-layer detection, the optimized method stains the positive bacteria with Coomassie Brilliant Blue. The lipases are detected by a colorimetric biochemical reaction triggered by the degradation of p-nitrophenyl dodecanoate into a yellow product detectable by spectrophotometry, if the result is positive. Both of these tests show results in less than an hour. Finally, the protease activity is measured by clarification of a medium containing milk during an overnight bacterial growth. These new protocols provide opportunities for quicker characterization of A. salmonicida subsp. salmonicida strains and, particularly, provide more precise results.
    MeSH term(s) Aeromonas ; Aeromonas salmonicida ; Animals ; Fish Diseases ; Virulence ; Virulence Factors/genetics
    Chemical Substances Virulence Factors
    Language English
    Publishing date 2021-11-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 432109-1
    ISSN 1365-2761 ; 0140-7775
    ISSN (online) 1365-2761
    ISSN 0140-7775
    DOI 10.1111/jfd.13547
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 4276: Show issues

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  2. Article: Improvements of virulence factor phenotypic tests for Aeromonas salmonicida subsp. salmonicida, a major fish pathogen

    Girard, Sarah B. / Paquet, Valérie E. / Charette, Steve J.

    Journal of fish diseases. 2022 Jan., v. 45, no. 1

    2022  

    Abstract: Aeromonas salmonicida subspecies salmonicida, a fish pathogen, expresses various virulence factors such as an A‐layer, lipases and proteases during the infection process. Not all strains of this bacterium express the same virulence factors. It is ... ...

    Abstract Aeromonas salmonicida subspecies salmonicida, a fish pathogen, expresses various virulence factors such as an A‐layer, lipases and proteases during the infection process. Not all strains of this bacterium express the same virulence factors. It is important to be able to evaluate which factors are present when characterizing strains. The A‐layer and secreted lipases and proteases are usually detected by agar‐based tests that require long incubation (24 h and more) and may provide ambiguous results. In the present study, protocols have been optimized to determine the presence of these virulence factors using liquid tests. For A‐layer detection, the optimized method stains the positive bacteria with Coomassie Brilliant Blue. The lipases are detected by a colorimetric biochemical reaction triggered by the degradation of p‐nitrophenyl dodecanoate into a yellow product detectable by spectrophotometry, if the result is positive. Both of these tests show results in less than an hour. Finally, the protease activity is measured by clarification of a medium containing milk during an overnight bacterial growth. These new protocols provide opportunities for quicker characterization of A. salmonicida subsp. salmonicida strains and, particularly, provide more precise results.
    Keywords Aeromonas salmonicida subsp. salmonicida ; bacteria ; bacterial growth ; carboxylic ester hydrolases ; colorimetry ; enzyme activity ; fish ; liquids ; milk ; pathogens ; phenotype ; proteinases ; spectroscopy ; virulence
    Language English
    Dates of publication 2022-01
    Size p. 177-184.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 432109-1
    ISSN 1365-2761 ; 0140-7775
    ISSN (online) 1365-2761
    ISSN 0140-7775
    DOI 10.1111/jfd.13547
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 4276: Show issues

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  3. Article: N

    Cottin, Lola / Girard, Sarah / Hanan, Garry S / Cibian, Mihaela

    Acta crystallographica. Section E, Crystallographic communications

    2021  Volume 77, Issue Pt 2, Page(s) 117–120

    Abstract: The title compound, ... ...

    Abstract The title compound, C
    Language English
    Publishing date 2021-01-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 2041947-8
    ISSN 2056-9890 ; 1600-5368
    ISSN 2056-9890 ; 1600-5368
    DOI 10.1107/S2056989021000141
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Interaction of pAsa5 and pAsa8 Plasmids in

    Marcoux, Pierre-Étienne / Girard, Sarah B / Fournier, Kim C / Tardif, Catherine A / Gosselin, Ariane / Charette, Steve J

    Microorganisms

    2023  Volume 11, Issue 11

    Abstract: The plasmid known as pAsa5 is present ... ...

    Abstract The plasmid known as pAsa5 is present in
    Language English
    Publishing date 2023-11-02
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms11112685
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Host Dependent-Transposon for a Plasmid Found in

    Marcoux, Pierre-Étienne / Attéré, Sabrina A / Paquet, Valérie E / Paquet, Maude F / Girard, Sarah B / Farley, Judith / Frenette, Michel / Vincent, Antony T / Charette, Steve J

    Antibiotics (Basel, Switzerland)

    2023  Volume 12, Issue 2

    Abstract: Plasmids that carry antibiotic resistance genes occur frequently ... ...

    Abstract Plasmids that carry antibiotic resistance genes occur frequently in
    Language English
    Publishing date 2023-01-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2681345-2
    ISSN 2079-6382
    ISSN 2079-6382
    DOI 10.3390/antibiotics12020257
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Antimicrobial Resistance in the Environment: Towards Elucidating the Roles of Bioaerosols in Transmission and Detection of Antibacterial Resistance Genes.

    George, Paul B L / Rossi, Florent / St-Germain, Magali-Wen / Amato, Pierre / Badard, Thierry / Bergeron, Michel G / Boissinot, Maurice / Charette, Steve J / Coleman, Brenda L / Corbeil, Jacques / Culley, Alexander I / Gaucher, Marie-Lou / Girard, Matthieu / Godbout, Stéphane / Kirychuk, Shelley P / Marette, André / McGeer, Allison / O'Shaughnessy, Patrick T / Parmley, E Jane /
    Simard, Serge / Reid-Smith, Richard J / Topp, Edward / Trudel, Luc / Yao, Maosheng / Brassard, Patrick / Delort, Anne-Marie / Larios, Araceli D / Létourneau, Valérie / Paquet, Valérie E / Pedneau, Marie-Hélène / Pic, Émilie / Thompson, Brooke / Veillette, Marc / Thaler, Mary / Scapino, Ilaria / Lebeuf, Maria / Baghdadi, Mahsa / Castillo Toro, Alejandra / Cayouette, Amélia Bélanger / Dubois, Marie-Julie / Durocher, Alicia F / Girard, Sarah B / Diaz, Andrea Katherín Carranza / Khalloufi, Asmaâ / Leclerc, Samantha / Lemieux, Joanie / Maldonado, Manuel Pérez / Pilon, Geneviève / Murphy, Colleen P / Notling, Charly A / Ofori-Darko, Daniel / Provencher, Juliette / Richer-Fortin, Annabelle / Turgeon, Nathalie / Duchaine, Caroline

    Antibiotics (Basel, Switzerland)

    2022  Volume 11, Issue 7

    Abstract: Antimicrobial resistance (AMR) is continuing to grow across the world. Though often thought of as a mostly public health issue, AMR is also a major agricultural and environmental problem. As such, many researchers refer to it as the preeminent One Health ...

    Abstract Antimicrobial resistance (AMR) is continuing to grow across the world. Though often thought of as a mostly public health issue, AMR is also a major agricultural and environmental problem. As such, many researchers refer to it as the preeminent One Health issue. Aerial transport of antimicrobial-resistant bacteria via bioaerosols is still poorly understood. Recent work has highlighted the presence of antibiotic resistance genes in bioaerosols. Emissions of AMR bacteria and genes have been detected from various sources, including wastewater treatment plants, hospitals, and agricultural practices; however, their impacts on the broader environment are poorly understood. Contextualizing the roles of bioaerosols in the dissemination of AMR necessitates a multidisciplinary approach. Environmental factors, industrial and medical practices, as well as ecological principles influence the aerial dissemination of resistant bacteria. This article introduces an ongoing project assessing the presence and fate of AMR in bioaerosols across Canada. Its various sub-studies include the assessment of the emissions of antibiotic resistance genes from many agricultural practices, their long-distance transport, new integrative methods of assessment, and the creation of dissemination models over short and long distances. Results from sub-studies are beginning to be published. Consequently, this paper explains the background behind the development of the various sub-studies and highlight their shared aspects.
    Language English
    Publishing date 2022-07-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2681345-2
    ISSN 2079-6382
    ISSN 2079-6382
    DOI 10.3390/antibiotics11070974
    Database MEDical Literature Analysis and Retrieval System OnLINE

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