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  1. AU="Gismene, Carolina"
  2. AU="Jesus, Simal-Gandara"
  3. AU="Rumyantseva, N"
  4. AU="Torres, Gerardo M"
  5. AU="Qi, Nan"
  6. AU="Pinto, Daniel"
  7. AU="M Serrano-Martin"
  8. AU="Stefano Cacciaguerra"
  9. AU=Ochs Matthias
  10. AU="Ownby, Raymond L"
  11. AU="Belhouchat, Khadidja"
  12. AU="Hulsman, J"
  13. AU="Hadoke, Patrick W F"
  14. AU="Isermann, Philipp"
  15. AU="Polishchuk, Ilya"
  16. AU=Jalali Rostam
  17. AU="Baek, Kyoung Eun"
  18. AU="Gordon, Jay E"
  19. AU="Huang, Mingsheng"
  20. AU="Karkowski, L"
  21. AU="Rana, Punam"
  22. AU=Kawasaki Shunsuke
  23. AU="Oliveira-Júnior, Gersiel"
  24. AU="Alvarado-Aguilar, Pablo"
  25. AU="Kyrin R. Hanning" AU="Kyrin R. Hanning"
  26. AU="Teixidor, Francesc"
  27. AU=Minhas Jasmit
  28. AU="Poe, Alyssa"
  29. AU="Ni, Bo"
  30. AU="Vemuri, Kiranmayi"
  31. AU="Hernández-Wolters, Benjamin"
  32. AU="Chobanova, Veronika"
  33. AU="Otten, Gisela"
  34. AU="Elhence, Anshuman"
  35. AU="Tinneberg, H-R"
  36. AU="Puro, Neeraj"
  37. AU=Yang Chi-Fu Jeffrey
  38. AU="Oliveira, Giuliana S"
  39. AU="Maria Cecilia Jocson"
  40. AU="Tollman, Stephen"
  41. AU="Cherry, Katie E."
  42. AU="Nazet, Ute"
  43. AU="Kranzer, K"
  44. AU="Avelino, Samuel"
  45. AU="Sun, Xiang-Dong"
  46. AU="Vogl, Thomas J."
  47. AU="Johnson, C R"
  48. AU="Gil-Pérez, Pablo"
  49. AU="Donno, Federica"
  50. AU="Vom Saal, Frederick S."
  51. AU="Gazzana, S"
  52. AU="Viswanadha, Vijaya P"
  53. AU="Anastasi, G A"
  54. AU="Romerosa, Antonio"
  55. AU=Gupta Gaorav P
  56. AU="Fernández-Susavila, Héctor"

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  1. Artikel ; Online: Necrotic activity of ExhC from Mammaliicoccus sciuri is mediated by specific amino acid residues.

    Gismene, Carolina / González, Jorge Enrique Hernández / de Freitas Calmon, Marília / Nascimento, Andrey Fabricio Ziem / Santisteban, Angela Rocio Niño / Calil, Felipe Antunes / da Silva, Alana Della Torre / Rahal, Paula / Góes, Rejane Maira / Arni, Raghuvir Krishnaswamy / Mariutti, Ricardo Barros

    International journal of biological macromolecules

    2023  Band 254, Heft Pt 1, Seite(n) 127741

    Abstract: Mammaliicoccus sciuri, a commensal and pathogenic bacterium of significant clinical and veterinary relevance, expresses exfoliative toxin C (ExhC), a specific glutamyl endopeptidase belonging to the chymotrypsin family as the principal virulence factor. ... ...

    Abstract Mammaliicoccus sciuri, a commensal and pathogenic bacterium of significant clinical and veterinary relevance, expresses exfoliative toxin C (ExhC), a specific glutamyl endopeptidase belonging to the chymotrypsin family as the principal virulence factor. However, unlike most members of this family, ETs are inactive against a wide range of substrates and possess exquisite specificity for desmoglein-1 (Dsg1), a cadherin-like adhesion molecule that is crucial to maintain tissue integrity, thereby preventing the separation of skin cells and the entry of pathogens. ExhC is of clinical importance since in addition to causing exfoliation in pigs and mice, it induces necrosis in multiple mammalian cell lines, a property not observed for other ETs. Previous experiments have implicated the ExhC
    Mesh-Begriff(e) Animals ; Swine ; Mice ; Amino Acids/metabolism ; Exfoliatins/genetics ; Exfoliatins/metabolism ; Exfoliatins/pharmacology ; Staphylococcus ; Necrosis ; Mammals/metabolism
    Chemische Substanzen Amino Acids ; Exfoliatins
    Sprache Englisch
    Erscheinungsdatum 2023-10-29
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2023.127741
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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    Verfügbar in ZB MED Köln/Königswinter

    Zs.B 2374: Hefte anzeigen Standort:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
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  2. Artikel ; Online: Immunoprophylactic properties of the Corynebacterium pseudotuberculosis-derived MBP:PLD:CP40 fusion protein

    Barral, Thiago Doria / Kalil, Mauricio Alcantara / Mariutti, Ricardo Barros / Arni, Raghuvir Krishnaswamy / Gismene, Carolina / Sousa, Fernanda Severo / Collares, Tiago / Seixas, Fabiana Kommling / Borsuk, Sibele / Estrela-Lima, Alessandra / Azevedo, Vasco / Meyer, Roberto / Portela, Ricardo Wagner

    Appl Microbiol Biotechnol. 2022 Dec., v. 106, no. 24 p.8035-8051

    2022  

    Abstract: Caseous lymphadenitis (CLA) is a disease that affects small ruminants, and the best way to prevent its spread on a herd is through immunoprophylaxis. Thus, we aimed to evaluate the MBP:PLD:CP40 fusion protein as a new CLA immunogen. The fusion protein ... ...

    Abstract Caseous lymphadenitis (CLA) is a disease that affects small ruminants, and the best way to prevent its spread on a herd is through immunoprophylaxis. Thus, we aimed to evaluate the MBP:PLD:CP40 fusion protein as a new CLA immunogen. The fusion protein was constructed by combining Corynebacterium pseudotuberculosis PLD and CP40 proteins with maltose-binding protein (MBP) as an intrinsic adjuvant. The antigenicity, allergenic potential, prediction of B epitopes, binding to MHC receptors, and docking on the Toll-Like 2 receptor were evaluated in silico. MBP:PLD:CP40 was expressed and purified. 40 BALB/c were divided into four groups (G1 – control, G2 – Saponin, G3 – MBP:PLD:CP40, and G4 – rPLD + rCP40). Total IgG, IgG1, and IgG2a were quantified, and the expressions of cytokines after splenocyte in vitro stimulation were assessed. Mice were challenged 42 days after the first immunization. The in silico analysis showed that MBP:PLD:CP40 has immunogenic potential, does not have allergic properties, and can dock on the TRL2 receptor. MBP:PLD:CP40 stimulated the production of IgG1 antibodies in a fivefold proportion to IgG2a, and TNF and IL-17 were significantly expressed in response to the antigenic stimuli. When rPLD and rCP40 were used together for immunization, they could induce IFN-γ and IL-12, but with no detectable antibody production. The G3 and G4 groups presented a survival of 57.14% and 42.86%, respectively, while the G1 and G2 mice were all dead 15 days after the challenge. MBP:PLD:CP40 partially protected the mice against C. pseudotuberculosis infection and can be considered a potential new CLA immunogen. KEY POINTS: • The fusion protein induced more IgG1 than IgG2a antibodies; • The fusion protein also induced the expression of the TNF and IL-17 cytokines; • Mice inoculated with MBP:PLD:CP40 presented a 57.14% survival.
    Schlagwörter Corynebacterium pseudotuberculosis ; adjuvants ; allergenicity ; antibody formation ; caseous lymphadenitis ; computer simulation ; epitopes ; herds ; immunization ; immunoglobulin G ; interleukin-12 ; interleukin-17 ; prediction ; saponins ; splenocytes
    Sprache Englisch
    Erscheinungsverlauf 2022-12
    Umfang p. 8035-8051.
    Erscheinungsort Springer Berlin Heidelberg
    Dokumenttyp Artikel ; Online
    ZDB-ID 392453-1
    ISSN 1432-0614 ; 0171-1741 ; 0175-7598
    ISSN (online) 1432-0614
    ISSN 0171-1741 ; 0175-7598
    DOI 10.1007/s00253-022-12279-1
    Datenquelle NAL Katalog (AGRICOLA)

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    Verfügbar in ZB MED Bonn

    Z 79/727: Hefte anzeigen

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  3. Artikel ; Online: Immunoprophylactic properties of the Corynebacterium pseudotuberculosis-derived MBP:PLD:CP40 fusion protein.

    Barral, Thiago Doria / Kalil, Mauricio Alcantara / Mariutti, Ricardo Barros / Arni, Raghuvir Krishnaswamy / Gismene, Carolina / Sousa, Fernanda Severo / Collares, Tiago / Seixas, Fabiana Kommling / Borsuk, Sibele / Estrela-Lima, Alessandra / Azevedo, Vasco / Meyer, Roberto / Portela, Ricardo Wagner

    Applied microbiology and biotechnology

    2022  Band 106, Heft 24, Seite(n) 8035–8051

    Abstract: Caseous lymphadenitis (CLA) is a disease that affects small ruminants, and the best way to prevent its spread on a herd is through immunoprophylaxis. Thus, we aimed to evaluate the MBP:PLD:CP40 fusion protein as a new CLA immunogen. The fusion protein ... ...

    Abstract Caseous lymphadenitis (CLA) is a disease that affects small ruminants, and the best way to prevent its spread on a herd is through immunoprophylaxis. Thus, we aimed to evaluate the MBP:PLD:CP40 fusion protein as a new CLA immunogen. The fusion protein was constructed by combining Corynebacterium pseudotuberculosis PLD and CP40 proteins with maltose-binding protein (MBP) as an intrinsic adjuvant. The antigenicity, allergenic potential, prediction of B epitopes, binding to MHC receptors, and docking on the Toll-Like 2 receptor were evaluated in silico. MBP:PLD:CP40 was expressed and purified. 40 BALB/c were divided into four groups (G1 - control, G2 - Saponin, G3 - MBP:PLD:CP40, and G4 - rPLD + rCP40). Total IgG, IgG1, and IgG2a were quantified, and the expressions of cytokines after splenocyte in vitro stimulation were assessed. Mice were challenged 42 days after the first immunization. The in silico analysis showed that MBP:PLD:CP40 has immunogenic potential, does not have allergic properties, and can dock on the TRL2 receptor. MBP:PLD:CP40 stimulated the production of IgG1 antibodies in a fivefold proportion to IgG2a, and TNF and IL-17 were significantly expressed in response to the antigenic stimuli. When rPLD and rCP40 were used together for immunization, they could induce IFN-γ and IL-12, but with no detectable antibody production. The G3 and G4 groups presented a survival of 57.14% and 42.86%, respectively, while the G1 and G2 mice were all dead 15 days after the challenge. MBP:PLD:CP40 partially protected the mice against C. pseudotuberculosis infection and can be considered a potential new CLA immunogen. KEY POINTS: • The fusion protein induced more IgG1 than IgG2a antibodies; • The fusion protein also induced the expression of the TNF and IL-17 cytokines; • Mice inoculated with MBP:PLD:CP40 presented a 57.14% survival.
    Mesh-Begriff(e) Animals ; Mice ; Corynebacterium pseudotuberculosis/genetics ; Maltose-Binding Proteins ; Interleukin-17
    Chemische Substanzen Maltose-Binding Proteins ; Interleukin-17
    Sprache Englisch
    Erscheinungsdatum 2022-11-14
    Erscheinungsland Germany
    Dokumenttyp Journal Article
    ZDB-ID 392453-1
    ISSN 1432-0614 ; 0171-1741 ; 0175-7598
    ISSN (online) 1432-0614
    ISSN 0171-1741 ; 0175-7598
    DOI 10.1007/s00253-022-12279-1
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

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    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 2229: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Artikel ; Online: Staphylococcus aureus

    Gismene, Carolina / Hernández González, Jorge Enrique / Santisteban, Angela Rocio Niño / Ziem Nascimento, Andrey Fabricio / Dos Santos Cunha, Lucas / de Moraes, Fábio Rogério / de Oliveira, Cristiano Luis Pinto / Oliveira, Caio C / Jocelan Scarin Provazzi, Paola / Pascutti, Pedro Geraldo / Arni, Raghuvir Krishnaswamy / Barros Mariutti, Ricardo

    International journal of molecular sciences

    2022  Band 23, Heft 17

    Abstract: Staphylococcal exfoliative toxins (ETs) are glutamyl endopeptidases that specifically cleave the Glu381-Gly382 bond in the ectodomains of desmoglein 1 (Dsg1) via complex action mechanisms. To date, four ETs have been identified in ... ...

    Abstract Staphylococcal exfoliative toxins (ETs) are glutamyl endopeptidases that specifically cleave the Glu381-Gly382 bond in the ectodomains of desmoglein 1 (Dsg1) via complex action mechanisms. To date, four ETs have been identified in different
    Mesh-Begriff(e) Catalytic Domain ; Exfoliatins/chemistry ; Exfoliatins/metabolism ; Humans ; Staphylococcal Infections ; Staphylococcus aureus/metabolism
    Chemische Substanzen Exfoliatins
    Sprache Englisch
    Erscheinungsdatum 2022-08-30
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms23179857
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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