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Book ; Online ; Thesis: Comparative cephalometric studies of the mandible in growing Göttingen Minipigs using 3D Computed Tomography: Refining experimental dental and orofacial research

2020

Author's details	Giuliano Corte
Keywords	Landwirtschaft, Veterinärmedizin ; Agriculture, Veterinary Science
Subject code	sg630
Language	English
Publisher	Freie Universität Berlin
Publishing place	Berlin
Document type	Book ; Online ; Thesis
Database	Digital theses on the web

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Article ; Online: Knockdown of TPI in human dermal microvascular endothelial cells and its impact on angiogenesis in vitro.

Herre, Christina / Nshdejan, Arpenik / Klopfleisch, Robert / Corte, Giuliano Mario / Bahramsoltani, Mahtab

PloS one

2023 Volume 18, Issue 12, Page(s) e0294933

Abstract: Introduction: Angiogenic behaviour has been shown as highly versatile among Endothelial cells (ECs) causing problems of in vitro assays of angiogenesis considering their reproducibility. It is indispensable to investigate influencing factors of the ... ...

Abstract	Introduction: Angiogenic behaviour has been shown as highly versatile among Endothelial cells (ECs) causing problems of in vitro assays of angiogenesis considering their reproducibility. It is indispensable to investigate influencing factors of the angiogenic potency of ECs. Objective: The present study aimed to analyse the impact of knocking down triosephosphate isomerase (TPI) on in vitro angiogenesis and simultaneously on vimentin (VIM) and adenosylmethionine synthetase isoform type 2 (MAT2A) expression. Furthermore, native expression profiles of TPI, VIM and MAT2A in the course of angiogenesis in vitro were examined. Methods: Two batches of human dermal microvascular ECs were cultivated over 50 days and stimulated to undergo angiogenesis. A shRNA-mediated knockdown of TPI was performed. During cultivation, time-dependant morphological changes were detected and applied for EC-staging as prerequisite for quantifying in vitro angiogenesis. Additionally, mRNA and protein levels of all proteins were monitored. Results: Opposed to native cells, knockdown cells were not able to enter late stages of angiogenesis and primarily displayed a downregulation of VIM and an uprise in MAT2A expression. Native cells increased their TPI expression and decreased their VIM expression during the course of angiogenesis in vitro. For MAT2A, highest expression was observed to be in the beginning and at the end of angiogenesis. Conclusion: Knocking down TPI provoked expressional changes in VIM and MAT2A and a deceleration of in vitro angiogenesis, indicating that TPI represents an angiogenic protein. Native expression profiles lead to the assumption of VIM being predominantly relevant in beginning stages, MAT2A in beginning and late stages and TPI during the whole course of angiogenesis in vitro.
MeSH term(s)	Humans ; Triose-Phosphate Isomerase/genetics ; Endothelial Cells/metabolism ; Reproducibility of Results ; Angiogenesis ; Down-Regulation ; Methionine Adenosyltransferase/metabolism
Chemical Substances	Triose-Phosphate Isomerase (EC 5.3.1.1) ; MAT2A protein, human (EC 2.5.1.6) ; Methionine Adenosyltransferase (EC 2.5.1.6)
Language	English
Publishing date	2023-12-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0294933
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Expression of vimentin, TPI and MAT2A in human dermal microvascular endothelial cells during angiogenesis in vitro.

Christina Herre / Arpenik Nshdejan / Robert Klopfleisch / Giuliano Mario Corte / Mahtab Bahramsoltani

PLoS ONE, Vol 17, Iss 4, p e

2022 Volume 0266774

Abstract: Introduction In vitro assays of angiogenesis face immense problems considering their reproducibility based on the inhomogeneous characters of endothelial cells (ECs). It is necessary to detect influencing factors, which affect the angiogenic potency of ... ...

Abstract	Introduction In vitro assays of angiogenesis face immense problems considering their reproducibility based on the inhomogeneous characters of endothelial cells (ECs). It is necessary to detect influencing factors, which affect the angiogenic potency of ECs. Objective This study aimed to analyse expression profiles of vimentin (VIM), triosephosphate isomerase (TPI) and adenosylmethionine synthetase isoform type-2 (MAT2A) during the whole angiogenic cascade in vitro. Furthermore, the impact of knocking down vimentin (VIM) on angiogenesis in vitro was evaluated, while monitoring TPI and MAT2A expression. Methods A long-term cultivation and angiogenic stimulation of human dermal microvascular ECs was performed. Cells were characterized via VEGFR-1 and VEGFR-2 expression and a shRNA-mediated knockdown of VIM was performed. The process of angiogenesis in vitro was quantified via morphological staging and mRNA-and protein-levels of all proteins were analysed. Results While native cells ran through the angiogenic cascade chronologically, knockdown cells only entered beginning stages of angiogenesis and died eventually. Cell cultures showing a higher VEGFR-1 expression survived exclusively and displayed an upregulation of MAT2A and TPI expression. Native cells highly expressed VIM in early stages, MAT2A mainly in the beginning and TPI during the course of angiogenesis in vitro. Conclusion VIM knockdown led to a deceleration of angiogenesis in vitro and knockdown cells displayed expressional changes in TPI and MAT2A. Cell populations with a higher number of stalk cells emerged as being more stable against manipulations and native expression profiles provided an indication of VIM and MAT2A being relevant predominantly in beginning stages and TPI during the whole angiogenic cascade in vitro.
Keywords	Medicine ; R ; Science ; Q
Subject code	570
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Expression of vimentin, TPI and MAT2A in human dermal microvascular endothelial cells during angiogenesis in vitro.

Herre, Christina / Nshdejan, Arpenik / Klopfleisch, Robert / Corte, Giuliano Mario / Bahramsoltani, Mahtab

PloS one

2022 Volume 17, Issue 4, Page(s) e0266774

Abstract: Introduction: In vitro assays of angiogenesis face immense problems considering their reproducibility based on the inhomogeneous characters of endothelial cells (ECs). It is necessary to detect influencing factors, which affect the angiogenic potency of ...

Abstract	Introduction: In vitro assays of angiogenesis face immense problems considering their reproducibility based on the inhomogeneous characters of endothelial cells (ECs). It is necessary to detect influencing factors, which affect the angiogenic potency of ECs. Objective: This study aimed to analyse expression profiles of vimentin (VIM), triosephosphate isomerase (TPI) and adenosylmethionine synthetase isoform type-2 (MAT2A) during the whole angiogenic cascade in vitro. Furthermore, the impact of knocking down vimentin (VIM) on angiogenesis in vitro was evaluated, while monitoring TPI and MAT2A expression. Methods: A long-term cultivation and angiogenic stimulation of human dermal microvascular ECs was performed. Cells were characterized via VEGFR-1 and VEGFR-2 expression and a shRNA-mediated knockdown of VIM was performed. The process of angiogenesis in vitro was quantified via morphological staging and mRNA-and protein-levels of all proteins were analysed. Results: While native cells ran through the angiogenic cascade chronologically, knockdown cells only entered beginning stages of angiogenesis and died eventually. Cell cultures showing a higher VEGFR-1 expression survived exclusively and displayed an upregulation of MAT2A and TPI expression. Native cells highly expressed VIM in early stages, MAT2A mainly in the beginning and TPI during the course of angiogenesis in vitro. Conclusion: VIM knockdown led to a deceleration of angiogenesis in vitro and knockdown cells displayed expressional changes in TPI and MAT2A. Cell populations with a higher number of stalk cells emerged as being more stable against manipulations and native expression profiles provided an indication of VIM and MAT2A being relevant predominantly in beginning stages and TPI during the whole angiogenic cascade in vitro.
MeSH term(s)	Endothelial Cells/metabolism ; Humans ; Methionine Adenosyltransferase/metabolism ; Neovascularization, Pathologic/genetics ; Neovascularization, Pathologic/metabolism ; Reproducibility of Results ; Triose-Phosphate Isomerase/metabolism ; Vascular Endothelial Growth Factor Receptor-1/metabolism ; Vimentin/genetics ; Vimentin/metabolism
Chemical Substances	Vimentin ; MAT2A protein, human (EC 2.5.1.6) ; Methionine Adenosyltransferase (EC 2.5.1.6) ; Vascular Endothelial Growth Factor Receptor-1 (EC 2.7.10.1) ; Triose-Phosphate Isomerase (EC 5.3.1.1)
Language	English
Publishing date	2022-04-28
Publishing country	United States
Document type	Journal Article
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0266774
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Ribociclib in newly diagnosed hepatitis B infection: A case report.

Di Costanzo, Fabrizio / Carrano, Simone / Iengo, Gennaro / Cefaliello, Amedeo / Cossiga, Valentina / Morisco, Filomena / Giuliano, Mario / De Angelis, Carmine / Arpino, Grazia

Frontiers in oncology

2023 Volume 13, Page(s) 1184952

Abstract: Breast cancer is the most frequently diagnosed cancer in women worldwide. Actually CDK4/6 inhibitor Ribociclib is approved for the treatment of metastatic hormone-positive and human epidermal growth factor receptor 2 (HER 2)-negative breast cancer, but ... ...

Abstract	Breast cancer is the most frequently diagnosed cancer in women worldwide. Actually CDK4/6 inhibitor Ribociclib is approved for the treatment of metastatic hormone-positive and human epidermal growth factor receptor 2 (HER 2)-negative breast cancer, but comorbidities like infectious or cardiovascular diseases may limit its use. Case report: A 45-year-old woman was diagnosed with metastatic breast cancer in September 2021; also, her hepatitis screening resulted positive for hepatitis B infection. Patient assumed eradicative therapy for hepatitis and bit after started oncological therapy with Ribociclib. Outcome: Frequent check of hepatological function was observed since start of eradicative therapy; liver transaminases and bilirubin kept to not rise despite start of oncological treatment with Ribociclib. Patient's Performance Status was also not compromised and revaluation at 4, 9 and 13 months showed partial response and then stable disease. Discussion: hepatotoxicity of Ribociclib is reported as a possible side effect, and often positivity for hepatitis is cause of exclusion from therapy; in our case, no hepatotoxicity was noted and patient obtained response in terms of control of both infectious and oncological diseases.
Language	English
Publishing date	2023-06-08
Publishing country	Switzerland
Document type	Case Reports
ZDB-ID	2649216-7
ISSN	2234-943X
ISSN	2234-943X
DOI	10.3389/fonc.2023.1184952
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Serum biomarkers of inflammation and vascular damage upon SARS-Cov-2 mRNA vaccine in patients with thymic epithelial tumors.

Cernera, Gustavo / Gelzo, Monica / De Placido, Pietro / Pietroluongo, Erica / Raia, Maddalena / Scalia, Giulia / Tortora, Marianna / Formisano, Pietro / Palmieri, Giovannella / Giuliano, Mario / Castaldo, Giuseppe

Clinical chemistry and laboratory medicine

2024 Volume 62, Issue 6, Page(s) 1198–1205

Abstract: Objectives: Thymic epithelial tumors (TET) patients are at high risk of autoimmune and hypoimmune complications. Limited evidence is available on the potential risk of immune-related and inflammatory reactions induced by SARS-Cov-2 vaccine in this ... ...

Abstract	Objectives: Thymic epithelial tumors (TET) patients are at high risk of autoimmune and hypoimmune complications. Limited evidence is available on the potential risk of immune-related and inflammatory reactions induced by SARS-Cov-2 vaccine in this patient population. Methods: In order to identify subjects at higher risk for vaccine complications, we prospectively evaluated a panel of serum biomarkers related to inflammation (TNF-α, IL-1β, -6, -10, -12, and -17A, IFN-α, β and γ, MPO, MMP-9), and vascular damage (E- and P-selectin, VEGF-A, P-ANCA and MCP-1) in 44 TET patients and in 30 healthy controls along the whole SARS-Cov-2 vaccine cycle. Results: About 50 % of subjects (either TET and controls) showed an increase of serum biochemical markers of inflammation and endothelial damage with a large heterogeneity of values. Such increase appeared early, after the first dose in control subjects and later, after the second dose in TET patients (in which we observed mainly an increase of inflammatory biomarkers). The values normalized after about 3 months and did not increase after the third, booster dose. No autoimmune or vascular complications were observed in the study subjects and no difference was observed in terms of vaccine response among subjects showing serum biomarkers increase and those who experienced no changes. Conclusions: Our data highlight the relevance of Sars-Cov-2 vaccine in TET patients, as it resulted safe and prevented severe COVID-19. However, further studies are awaited to explore the mechanisms and the potential consequences of the observed increase of serum inflammatory and vascular damage biomarkers.
MeSH term(s)	Humans ; Male ; Middle Aged ; Female ; Biomarkers/blood ; Inflammation/blood ; Aged ; Thymus Neoplasms/blood ; Thymus Neoplasms/immunology ; COVID-19 Vaccines/adverse effects ; COVID-19/blood ; COVID-19/prevention & control ; Adult ; Neoplasms, Glandular and Epithelial/blood ; SARS-CoV-2/immunology ; Prospective Studies ; mRNA Vaccines
Chemical Substances	Biomarkers ; COVID-19 Vaccines ; mRNA Vaccines
Language	English
Publishing date	2024-01-18
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1418007-8
ISSN	1437-4331 ; 1434-6621 ; 1437-8523
ISSN (online)	1437-4331
ISSN	1434-6621 ; 1437-8523
DOI	10.1515/cclm-2023-1283
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Task force veterinary anatomy: joint efforts of the five German veterinary schools to ensure education during the COVID-19 pandemic.

Bernigau, Dora / Bahramsoltani, Mahtab / Corte, Giuliano Mario / Reese, Sven / Pfarrer, Christiane / Fietz, Daniela

GMS journal for medical education

2021 Volume 38, Issue 5, Page(s) Doc87

Abstract: At the start of the COVID-pandemic in March 2020, the Institutes of Veterinary Anatomy of the five German educational institutions were confronted with the challenge of digitalising all lectures for the second and fourth semesters of veterinary students. ...

Abstract	At the start of the COVID-pandemic in March 2020, the Institutes of Veterinary Anatomy of the five German educational institutions were confronted with the challenge of digitalising all lectures for the second and fourth semesters of veterinary students. After an online kick-off event and a preliminary status quo meeting, available digital teaching material was exchanged for students to stream from learning platforms. Lectures were either synchronized or made available as audio recordings and connotated slides on the learning platforms. Fortunately, digital microscopic slides had already been in use, which made it easy for students to access them. Dissection exercises mostly consisted of self-study, using instructive videos and interactive exercises. In the second half of the semester, four of the educational institutions were able to offer a restricted number of in-person gross anatomy classes under reinforced conditions. Success monitoring took place online through different formats, and partially on a voluntary basis, via the learning platforms. Although the past two semesters had to almost exclusively take place online due to the unprecedented circumstances, and joint efforts of the five veterinary institutions, there is a general consensus that the practical education in anatomy, histology and embryology is essential to veterinary students. In fact, it is the only way they can obtain the necessary skills to successfully complete the rest of their degree.
MeSH term(s)	Anatomy/education ; Audiovisual Aids ; COVID-19 ; Digital Technology ; Dissection ; Education, Distance ; Education, Veterinary/methods ; Germany ; Humans ; Internet ; Microscopy ; Pandemics ; Physical Distancing ; Professional Competence ; Schools, Veterinary ; Students ; Teaching ; Universities ; Veterinary Medicine
Language	English
Publishing date	2021-06-15
Publishing country	Germany
Document type	Journal Article
ISSN	2366-5017
ISSN (online)	2366-5017
DOI	10.3205/zma001483
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Reliability of the Mouse Grimace Scale in C57BL/6JRj Mice

Katharina Hohlbaum / Giuliano Mario Corte / Melanie Humpenöder / Roswitha Merle / Christa Thöne-Reineke

Animals, Vol 10, Iss 1648, p

2020 Volume 1648

Abstract: To maintain and foster the welfare of laboratory mice, tools that reliably measure the current state of the animals are applied in clinical assessment. One of these is the Mouse Grimace Scale (MGS), a coding system for facial expression analysis. Since ... ...

Abstract	To maintain and foster the welfare of laboratory mice, tools that reliably measure the current state of the animals are applied in clinical assessment. One of these is the Mouse Grimace Scale (MGS), a coding system for facial expression analysis. Since there are concerns about the objectivity of the MGS, we further investigated its reliability. Four observers (two experienced and two inexperienced in use of the MGS) scored 188 images of 33 female and 31 male C57BL/6JRj mice. Images were generated prior to, 150 min, and two days after ketamine/xylazine anesthesia. The intraclass correlations coefficient (ICC = 0.851) indicated good agreement on total MGS scores between all observers when all three time points were included in the analysis. However, interrater reliability was higher in the early post-anesthetic period (ICC = 0.799) than at baseline (ICC = 0.556) and on day 2 after anesthesia (ICC = 0.329). The best agreement was achieved for orbital tightening, and the poorest agreement for nose and cheek bulge, depending on the observers’ experience levels. In general, experienced observers produced scores of higher consistency when compared to inexperienced. Against this background, we critically discuss factors that potentially influence the reliability of MGS scoring.
Keywords	laboratory mice ; animal welfare indicators ; Mouse Grimace Scale ; intraclass correlations coefficient ; Fleiss’ kappa ; Cohen’s kappa ; Veterinary medicine ; SF600-1100 ; Zoology ; QL1-991
Language	English
Publishing date	2020-09-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Reliability of the Mouse Grimace Scale in C57BL/6JRj Mice

Hohlbaum, Katharina / Corte, Giuliano Mario / Humpenöder, Melanie / Merle, Roswitha / Thöne-Reineke, Christa

Animals. 2020 Sept. 14, v. 10, no. 9

2020

Abstract	To maintain and foster the welfare of laboratory mice, tools that reliably measure the current state of the animals are applied in clinical assessment. One of these is the Mouse Grimace Scale (MGS), a coding system for facial expression analysis. Since there are concerns about the objectivity of the MGS, we further investigated its reliability. Four observers (two experienced and two inexperienced in use of the MGS) scored 188 images of 33 female and 31 male C57BL/6JRj mice. Images were generated prior to, 150 min, and two days after ketamine/xylazine anesthesia. The intraclass correlations coefficient (ICC = 0.851) indicated good agreement on total MGS scores between all observers when all three time points were included in the analysis. However, interrater reliability was higher in the early post-anesthetic period (ICC = 0.799) than at baseline (ICC = 0.556) and on day 2 after anesthesia (ICC = 0.329). The best agreement was achieved for orbital tightening, and the poorest agreement for nose and cheek bulge, depending on the observers’ experience levels. In general, experienced observers produced scores of higher consistency when compared to inexperienced. Against this background, we critically discuss factors that potentially influence the reliability of MGS scoring.
Keywords	anesthesia ; females ; grimace scale ; ketamine ; males ; mice ; nose ; xylazine
Language	English
Dates of publication	2020-0914
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
Note	NAL-light
ISSN	2076-2615
DOI	10.3390/ani10091648
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Estrogen deprivation effects of endocrine therapy in breast cancer patients: Incidence, management and outcome.

Cucciniello, Linda / Garufi, Giovanna / Di Rienzo, Rossana / Martinelli, Claudia / Pavone, Giuliana / Giuliano, Mario / Arpino, Grazia / Montemurro, Filippo / Del Mastro, Lucia / De Laurentiis, Michelino / Puglisi, Fabio

Cancer treatment reviews

2023 Volume 120, Page(s) 102624

Abstract: Endocrine therapy is one of the standard adjuvant treatments to reduce the risk of recurrence and mortality in patients with hormone receptor positive early breast cancer. Despite its proven efficacy, ET side effects, which persist over time even if low ... ...

Abstract	Endocrine therapy is one of the standard adjuvant treatments to reduce the risk of recurrence and mortality in patients with hormone receptor positive early breast cancer. Despite its proven efficacy, ET side effects, which persist over time even if low grade, may deteriorate quality of life. During follow-up visits, emphasis is generally placed on the risk of disease recurrence, while the topic of ET side effects is commonly neglected and discussed only briefly. This could lead to poor adherence to therapy and early treatment discontinuation, resulting in worse survival outcomes. The aim of this review is to provide an overview of the available evidence on the incidence and reporting of ET-related side effects (including vasomotor symptoms, musculoskeletal disorders and genitourinary syndrome of menopause, as well as fatigue, psychological and ocular disorders, dysmetabolic effects and loss of bone density) and of the pharmacological and non-pharmacological strategies available to mitigate symptom burden.
MeSH term(s)	Female ; Humans ; Breast Neoplasms/drug therapy ; Breast Neoplasms/etiology ; Tamoxifen ; Antineoplastic Agents, Hormonal/adverse effects ; Estrogens/therapeutic use ; Quality of Life ; Incidence ; Aromatase Inhibitors/adverse effects ; Chemotherapy, Adjuvant ; Neoplasm Recurrence, Local/drug therapy
Chemical Substances	Tamoxifen (094ZI81Y45) ; Antineoplastic Agents, Hormonal ; Estrogens ; Aromatase Inhibitors
Language	English
Publishing date	2023-09-07
Publishing country	Netherlands
Document type	Journal Article ; Review
ZDB-ID	125102-8
ISSN	1532-1967 ; 0305-7372
ISSN (online)	1532-1967
ISSN	0305-7372
DOI	10.1016/j.ctrv.2023.102624
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