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  1. Article: Ultraviolet-B Radiation Represses Primary Root Elongation by Inhibiting Cell Proliferation in the Meristematic Zone of Arabidopsis Seedlings.

    Sheridan, Maria Luján / Simonelli, Lucio / Giustozzi, Marisol / Casati, Paula

    Frontiers in plant science

    2022  Volume 13, Page(s) 829336

    Abstract: ... ...

    Abstract In
    Language English
    Publishing date 2022-03-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2022.829336
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Arabidopsis mediator subunit 17 connects transcription with DNA repair after UV‐B exposure

    Giustozzi, Marisol / Freytes, Santiago Nicolás / Jaskolowski, Aime / Lichy, Micaela / Mateos, Julieta / Falcone Ferreyra, María Lorena / Rosano, Germán L. / Cerdán, Pablo / Casati, Paula

    plant journal. 2022 May, v. 110, no. 4

    2022  

    Abstract: Mediator 17 (MED17) is a subunit of the Mediator complex that regulates transcription initiation in eukaryotic organisms. In yeast and humans, MED17 also participates in DNA repair, physically interacting with proteins of the nucleotide excision DNA ... ...

    Abstract Mediator 17 (MED17) is a subunit of the Mediator complex that regulates transcription initiation in eukaryotic organisms. In yeast and humans, MED17 also participates in DNA repair, physically interacting with proteins of the nucleotide excision DNA repair system, but this function in plants has not been investigated. We studied the role of MED17 in Arabidopsis plants exposed to UV‐B radiation. Our results demonstrate that med17 and OE MED17 plants have altered responses to UV‐B, and that MED17 participates in various aspects of the DNA damage response (DDR). Comparison of the med17 transcriptome with that of wild‐type (WT) plants showed that almost one‐third of transcripts with altered expression in med17 plants were also changed by UV‐B exposure in WT plants. Increased sensitivity to DNA damage after UV‐B in med17 plants could result from the altered regulation of UV‐B responsive transcripts but MED17 also physically interacts with DNA repair proteins, suggesting a direct role of this Mediator subunit during repair. Finally, we show that MED17 is necessary to regulate the DDR activated by ataxia telangiectasia and Rad3 related (ATR), and that programmed cell death 5 (PDCD5) overexpression reverts the deficiencies in DDR shown in med17 mutants. Our data demonstrate that MED17 is an important regulator of DDR after UV‐B irradiation in Arabidopsis.
    Keywords Arabidopsis ; DNA damage ; DNA repair ; excision ; irradiation ; programmed cell death ; transcription initiation ; transcriptome ; yeasts
    Language English
    Dates of publication 2022-05
    Size p. 1047-1067.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 1088037-9
    ISSN 1365-313X ; 0960-7412
    ISSN (online) 1365-313X
    ISSN 0960-7412
    DOI 10.1111/tpj.15722
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    In stock of ZB MED Bonn / Germany

    Z 6071: Show issues

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  3. Article ; Online: Arabidopsis mediator subunit 17 connects transcription with DNA repair after UV-B exposure.

    Giustozzi, Marisol / Freytes, Santiago Nicolás / Jaskolowski, Aime / Lichy, Micaela / Mateos, Julieta / Falcone Ferreyra, María Lorena / Rosano, Germán L / Cerdán, Pablo / Casati, Paula

    The Plant journal : for cell and molecular biology

    2022  Volume 110, Issue 4, Page(s) 1047–1067

    Abstract: Mediator 17 (MED17) is a subunit of the Mediator complex that regulates transcription initiation in eukaryotic organisms. In yeast and humans, MED17 also participates in DNA repair, physically interacting with proteins of the nucleotide excision DNA ... ...

    Abstract Mediator 17 (MED17) is a subunit of the Mediator complex that regulates transcription initiation in eukaryotic organisms. In yeast and humans, MED17 also participates in DNA repair, physically interacting with proteins of the nucleotide excision DNA repair system, but this function in plants has not been investigated. We studied the role of MED17 in Arabidopsis plants exposed to UV-B radiation. Our results demonstrate that med17 and OE MED17 plants have altered responses to UV-B, and that MED17 participates in various aspects of the DNA damage response (DDR). Comparison of the med17 transcriptome with that of wild-type (WT) plants showed that almost one-third of transcripts with altered expression in med17 plants were also changed by UV-B exposure in WT plants. Increased sensitivity to DNA damage after UV-B in med17 plants could result from the altered regulation of UV-B responsive transcripts but MED17 also physically interacts with DNA repair proteins, suggesting a direct role of this Mediator subunit during repair. Finally, we show that MED17 is necessary to regulate the DDR activated by ataxia telangiectasia and Rad3 related (ATR), and that programmed cell death 5 (PDCD5) overexpression reverts the deficiencies in DDR shown in med17 mutants. Our data demonstrate that MED17 is an important regulator of DDR after UV-B irradiation in Arabidopsis.
    MeSH term(s) Arabidopsis/metabolism ; Arabidopsis Proteins/metabolism ; DNA Damage ; DNA Repair/genetics ; Ultraviolet Rays
    Chemical Substances Arabidopsis Proteins
    Language English
    Publishing date 2022-04-04
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1088037-9
    ISSN 1365-313X ; 0960-7412
    ISSN (online) 1365-313X
    ISSN 0960-7412
    DOI 10.1111/tpj.15722
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Bonn / Germany

    Z 6071: Show issues

    Order via subito

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    Inter-library loan at ZB MED

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  4. Article: CURLY LEAF Regulates MicroRNA Activity by Controlling ARGONAUTE 1 Degradation in Plants

    Ré, Delfina A / Arce, Agustin L / Ariel, Federico D / Cambiagno, Damian A / Casati, Paula / Giustozzi, Marisol / Manavella, Pablo A / Tomassi, Ariel H

    Molecular plant. 2020 Jan. 06, v. 13, no. 1

    2020  

    Abstract: CURLY LEAF (CLF) encodes the methyltransferase subunit of the Polycomb Repressor Complex 2 (PRC2), which regulates the expression of target genes through H3K27 trimethylation. We isolated a new CLF mutant allele (clf-78) using a genetic screen designed ... ...

    Abstract CURLY LEAF (CLF) encodes the methyltransferase subunit of the Polycomb Repressor Complex 2 (PRC2), which regulates the expression of target genes through H3K27 trimethylation. We isolated a new CLF mutant allele (clf-78) using a genetic screen designed to identify microRNA (miRNA) deficient mutants. CLF mutant plants showed impaired miRNA activity caused by increased ubiquitination and enhanced degradation of ARGONAUTE 1 (AGO1) in specific tissues. Such CLF-mediated AGO1 regulation was evident when plants were exposed to UV radiation, which caused increased susceptibility of clf mutants to some UV-induced responses. Furthermore, we showed that CLF directly regulates FBW2, which in turn triggers AGO1 degradation in the clf mutants. Interestingly, AGO1 bound to a target appeared particularly prone to degradation in the mutant plants, a process that was exacerbated when the complex bound a non-cleavable target. Thus, prolonged AGO1–target interaction seems to favor AGO1 degradation, suggesting that non-cleavable miRNA targets may overcome translation inhibition by modulating AGO1 stability in plants.
    Keywords alleles ; leaves ; methyltransferases ; microRNA ; mutants ; tissues ; ubiquitination ; ultraviolet radiation
    Language English
    Dates of publication 2020-0106
    Size p. 72-87.
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 2393618-6
    ISSN 1752-9867 ; 1674-2052
    ISSN (online) 1752-9867
    ISSN 1674-2052
    DOI 10.1016/j.molp.2019.10.003
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  5. Article ; Online: CURLY LEAF Regulates MicroRNA Activity by Controlling ARGONAUTE 1 Degradation in Plants.

    Ré, Delfina A / Cambiagno, Damian A / Arce, Agustin L / Tomassi, Ariel H / Giustozzi, Marisol / Casati, Paula / Ariel, Federico D / Manavella, Pablo A

    Molecular plant

    2019  Volume 13, Issue 1, Page(s) 72–87

    Abstract: CURLY LEAF (CLF) encodes the methyltransferase subunit of the Polycomb Repressor Complex 2 (PRC2), which regulates the expression of target genes through H3K27 trimethylation. We isolated a new CLF mutant allele (clf-78) using a genetic screen designed ... ...

    Abstract CURLY LEAF (CLF) encodes the methyltransferase subunit of the Polycomb Repressor Complex 2 (PRC2), which regulates the expression of target genes through H3K27 trimethylation. We isolated a new CLF mutant allele (clf-78) using a genetic screen designed to identify microRNA (miRNA) deficient mutants. CLF mutant plants showed impaired miRNA activity caused by increased ubiquitination and enhanced degradation of ARGONAUTE 1 (AGO1) in specific tissues. Such CLF-mediated AGO1 regulation was evident when plants were exposed to UV radiation, which caused increased susceptibility of clf mutants to some UV-induced responses. Furthermore, we showed that CLF directly regulates FBW2, which in turn triggers AGO1 degradation in the clf mutants. Interestingly, AGO1 bound to a target appeared particularly prone to degradation in the mutant plants, a process that was exacerbated when the complex bound a non-cleavable target. Thus, prolonged AGO1-target interaction seems to favor AGO1 degradation, suggesting that non-cleavable miRNA targets may overcome translation inhibition by modulating AGO1 stability in plants.
    MeSH term(s) Alleles ; Arabidopsis/genetics ; Arabidopsis/metabolism ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Argonaute Proteins/genetics ; Argonaute Proteins/metabolism ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Plant ; Genes, Plant ; Histones/metabolism ; Homeodomain Proteins/genetics ; Homeodomain Proteins/metabolism ; MicroRNAs/genetics ; Mutation ; Phenotype ; Plant Leaves/growth & development ; Plant Leaves/metabolism ; Polycomb-Group Proteins/metabolism
    Chemical Substances AGO1 protein, Arabidopsis ; Arabidopsis Proteins ; Argonaute Proteins ; CURLY LEAF protein, Arabidopsis ; Histones ; Homeodomain Proteins ; MicroRNAs ; Polycomb-Group Proteins
    Language English
    Publishing date 2019-10-10
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2393618-6
    ISSN 1752-9867 ; 1674-2052
    ISSN (online) 1752-9867
    ISSN 1674-2052
    DOI 10.1016/j.molp.2019.10.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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