Article ; Online: An integrated proteome and transcriptome of B cell maturation defines poised activation states of transitional and mature B cells.
2023 Volume 14, Issue 1, Page(s) 5116
Abstract: During B cell maturation, transitional and mature B cells acquire cell-intrinsic features that determine their ability to exit quiescence and mount effective immune responses. Here we use label-free proteomics to quantify the proteome of B cell subsets ... ...
| Abstract | During B cell maturation, transitional and mature B cells acquire cell-intrinsic features that determine their ability to exit quiescence and mount effective immune responses. Here we use label-free proteomics to quantify the proteome of B cell subsets from the mouse spleen and map the differential expression of environmental sensing, transcription, and translation initiation factors that define cellular identity and function. Cross-examination of the full-length transcriptome and proteome identifies mRNAs related to B cell activation and antibody secretion that are not accompanied by detection of the encoded proteins. In addition, proteomic data further suggests that the translational repressor PDCD4 restrains B cell responses, in particular those from marginal zone B cells, to a T-cell independent antigen. In summary, our molecular characterization of B cell maturation presents a valuable resource to further explore the mechanisms underpinning the specialized functions of B cell subsets, and suggest the presence of 'poised' mRNAs that enable expedited B cell responses. |
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| MeSH term(s) | B-Lymphocytes/cytology ; B-Lymphocytes/metabolism ; Proteome ; Transcriptome ; Animals ; Mice ; Cell Differentiation ; Transcription Factors/metabolism ; RNA, Messenger ; Protein Biosynthesis ; B-Lymphocyte Subsets/metabolism |
| Chemical Substances | Proteome ; Transcription Factors ; RNA, Messenger ; Pdcd4 protein, mouse |
| Language | English |
| Publishing date | 2023-08-23 |
| Publishing country | England |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2553671-0 |
| ISSN | 2041-1723 ; 2041-1723 |
| ISSN (online) | 2041-1723 |
| ISSN | 2041-1723 |
| DOI | 10.1038/s41467-023-40621-2 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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