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  1. Article ; Online: Decreased prolyl hydroxylase 3 mRNA expression in oncocytomas compared with clear cell renal cell carcinoma.

    Kampantais, Spyridon / Kounatidis, Ilias / Kotoula, Vasiliki / Vakalopoulos, Ioannis / Gkagkalidis, Konstantinos / Dimitriadis, Georgios

    The International journal of biological markers

    2020  Volume 35, Issue 4, Page(s) 80–86

    Abstract: Introduction: Hypoxia inducible factors (HIF) and prolyl hydroxylase domain (PHD) enzymes play a central role in tumor progression in clear cell renal cell carcinoma (ccRCC). However, there are currently no data regarding the behavior of this pathway ( ... ...

    Abstract Introduction: Hypoxia inducible factors (HIF) and prolyl hydroxylase domain (PHD) enzymes play a central role in tumor progression in clear cell renal cell carcinoma (ccRCC). However, there are currently no data regarding the behavior of this pathway (HIF/PHD) in a large number of benign renal tumors, the oncocytomas. The aim of the present study was to compare the expression levels of these factors between ccRCC and oncocytoma tumors.
    Material and methods: A total of 56 fresh frozen specimens from patients with ccRCC and 14 oncocytoma specimens were analyzed via reverse transcription-quantitative polymerase chain reaction in order to assess the expression levels of HIF-1α, HIF-2α, PHD1, PHD2, and PHD3. The analysis involved both fresh frozen tumor samples as well as adjacent normal kidney tissues.
    Results: In ccRCC, HIF-1α and HIF-2α levels were upregulated in 65.5% and 71.4% of cases, respectively. PHD3 was downregulated only in 15.4% of the ccRCC cases, in contrast with oncocytoma cases, which exhibited low expression levels in the majority. The upregulation of PHD3 messenger RNA (mRNA) levels in ccRCC when compared with oncocytoma was statistically significant (
    Conclusion: To the best of our knowledge, this is the first time that the HIF/PHD pathway was compared between ccRCC and a common benign tumor, identifying the upregulation of PHD3 as the possible underlying factor guiding the difference in the behavior of ccRCC.
    MeSH term(s) Adenoma, Oxyphilic/genetics ; Adenoma, Oxyphilic/metabolism ; Adult ; Aged ; Aged, 80 and over ; Basic Helix-Loop-Helix Transcription Factors/biosynthesis ; Basic Helix-Loop-Helix Transcription Factors/genetics ; Carcinoma, Renal Cell/genetics ; Carcinoma, Renal Cell/metabolism ; Female ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit/biosynthesis ; Hypoxia-Inducible Factor 1, alpha Subunit/genetics ; Hypoxia-Inducible Factor-Proline Dioxygenases/genetics ; Hypoxia-Inducible Factor-Proline Dioxygenases/metabolism ; Kidney Neoplasms/genetics ; Kidney Neoplasms/metabolism ; Male ; Middle Aged ; Prolyl Hydroxylases/genetics ; Prolyl Hydroxylases/metabolism ; Prospective Studies ; RNA, Messenger/biosynthesis ; RNA, Messenger/genetics ; RNA, Messenger/metabolism
    Chemical Substances Basic Helix-Loop-Helix Transcription Factors ; HIF1A protein, human ; Hypoxia-Inducible Factor 1, alpha Subunit ; RNA, Messenger ; endothelial PAS domain-containing protein 1 (1B37H0967P) ; Prolyl Hydroxylases (EC 1.14.11.-) ; EGLN3 protein, human (EC 1.14.11.29) ; Hypoxia-Inducible Factor-Proline Dioxygenases (EC 1.14.11.29)
    Language English
    Publishing date 2020-10-29
    Publishing country United States
    Document type Journal Article
    ZDB-ID 645113-5
    ISSN 1724-6008 ; 0393-6155
    ISSN (online) 1724-6008
    ISSN 0393-6155
    DOI 10.1177/1724600820960478
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2491: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Expression of HIF-2a in clear-cell renal cell carcinoma independently predicts overall survival.

    Gkagkalidis, Konstantinos / Kampantais, Spyridon / Dimitriadis, Georgios / Gourvas, Victoras / Kapoukranidou, Dorothea / Mironidou-Tzouveleki, Maria

    Medical molecular morphology

    2020  Volume 53, Issue 4, Page(s) 229–237

    Abstract: The purpose of this study is to evaluate the expression and the prognostic role of main factors, involved in the hypoxia pathway, in patients with clear-cell renal cell carcinoma (ccRCC). Immunohistochemical expression of Hypoxia inducible factors (HIF) ... ...

    Abstract The purpose of this study is to evaluate the expression and the prognostic role of main factors, involved in the hypoxia pathway, in patients with clear-cell renal cell carcinoma (ccRCC). Immunohistochemical expression of Hypoxia inducible factors (HIF) HIF-1a, HIF-2a, prolyl hydroxylases PHD1, PHD2, PHD3, and factor inhibiting HIF (FIH) was assessed on a tissue microarray, containing tumour and corresponding normal kidney tissue from 66 patients underwent surgery for ccRCC. Expression levels were evaluated in relation to T stage, Fuhrman grade, cancer-specific, and overall survival (OS). Cytoplasmatic expression of HIF-2a was positively correlated with expression of HIF-1a (p = 0.011). HIF-1a expression was also positively correlated with PHD3 and FIH (p = 0.020 and p = 0.039). Expression of HIF-1a was associated with lower Fuhrman grade (p = 0.008), while HIF-2a overexpression with unfavourable grade (p = 0.026). PHD3 was significant downregulated (84.8%). Age, LDH, presence of necrosis, Fuhrman grade, T stage, and HIF-2a cytoplasmatic expression were significant associated with OS of patients in univariable analysis. In multivariable analysis, HIF-2a expression (p = 0.006) and T stage (p = 0.001) remained as the only independent predictors for overall survival. These results indicate that HIF-2a overexpression not only is inversely correlated with Fuhrman grade in ccRCC, but also represents a strong independent prognostic factor for a poor overall survival.
    MeSH term(s) Aged ; Basic Helix-Loop-Helix Transcription Factors/genetics ; Biomarkers, Tumor/genetics ; Carcinoma, Renal Cell/diagnosis ; Carcinoma, Renal Cell/genetics ; Carcinoma, Renal Cell/mortality ; Carcinoma, Renal Cell/surgery ; Female ; Follow-Up Studies ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Humans ; Kaplan-Meier Estimate ; Kidney/pathology ; Kidney/surgery ; Kidney Neoplasms/diagnosis ; Kidney Neoplasms/genetics ; Kidney Neoplasms/mortality ; Kidney Neoplasms/surgery ; Male ; Middle Aged ; Neoplasm Staging ; Nephrectomy ; Prognosis ; Prospective Studies ; Risk Assessment/methods ; Tissue Array Analysis ; Tumor Microenvironment/genetics
    Chemical Substances Basic Helix-Loop-Helix Transcription Factors ; Biomarkers, Tumor ; endothelial PAS domain-containing protein 1 (1B37H0967P)
    Language English
    Publishing date 2020-03-26
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 2190059-0
    ISSN 1860-1499 ; 1860-1480
    ISSN (online) 1860-1499
    ISSN 1860-1480
    DOI 10.1007/s00795-020-00249-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4019: Show issues Location:
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    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Hypoxia-Inducible Factor 2a Expression Is Positively Correlated With Gleason Score in Prostate Cancer.

    Pavlakis, Dimitrios / Kampantais, Spyridon / Gkagkalidis, Konstantinos / Gourvas, Victoras / Memmos, Dimitrios / Tsionga, Aikaterini / Dimitriadis, Georgios / Vakalopoulos, Ioannis

    Technology in cancer research & treatment

    2021  Volume 20, Page(s) 1533033821990010

    Abstract: Background: One of the main factors in response to hypoxia in the tumor microenvironment is the hypoxia-inducible factor (HIF) pathway. Although its role in other solid tumors, particularly renal cell carcinoma, has been sufficiently elucidated, it ... ...

    Abstract Background: One of the main factors in response to hypoxia in the tumor microenvironment is the hypoxia-inducible factor (HIF) pathway. Although its role in other solid tumors, particularly renal cell carcinoma, has been sufficiently elucidated, it remains elusive in prostate cancer. The aim of the present study was to investigate the expression of main proteins involved in this pathway and determine the correlation of the results with clinicopathological outcomes of patients with prostate cancer.
    Methods: The immunohistochemical expression of HIF-1a, HIF-2a and their regulators, prolyl hydroxylase domain (PHD)1, PHD2 and PHD3 and factor inhibiting HIF (FIH), was assessed on a tissue microarray. This was constructed from radical prostatectomy specimens, involving both tumor and corresponding adjacent non-tumoral prostate tissues from 50 patients with localized or locally advanced prostate cancer.
    Results: In comparison with non-tumoral adjacent tissue, HIF-1a exhibited an equal or lower expression in 86% of the specimens (P = 0.017), while HIF-2a was overexpressed in 52% (P = 0.032) of the cases. HIF-1a protein expression was correlated with HIF-2a (P < 0.001), FIH (P = 0.004), PHD1 (P < 0.001), PHD2 (P < 0.001) and PHD3 (P = 0.035). HIF-2a expression was positively correlated with Gleason score (P = 0.017) and International Society of Urological Pathologists (ISUP) grade group (P = 0.022).
    Conclusions: The findings of the present study suggest a key role for HIF-2a in prostate cancer, as HIF-2a expression was found to be correlated with Gleason score and ISUP grade of the patients. However, further studies are required to validate these results and investigate the potential value of HIF-2a as a therapeutic target in prostate cancer.
    MeSH term(s) Aged ; Basic Helix-Loop-Helix Transcription Factors/metabolism ; Cell Hypoxia ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit/metabolism ; Hypoxia-Inducible Factor-Proline Dioxygenases/metabolism ; Male ; Middle Aged ; Mixed Function Oxygenases/metabolism ; Neoplasm Grading ; Prostate/metabolism ; Prostatic Neoplasms/metabolism ; Prostatic Neoplasms/pathology ; Repressor Proteins/metabolism ; Tumor Microenvironment
    Chemical Substances Basic Helix-Loop-Helix Transcription Factors ; HIF1A protein, human ; Hypoxia-Inducible Factor 1, alpha Subunit ; Repressor Proteins ; endothelial PAS domain-containing protein 1 (1B37H0967P) ; Mixed Function Oxygenases (EC 1.-) ; HIF1AN protein, human (EC 1.14.11.-) ; EGLN1 protein, human (EC 1.14.11.2) ; EGLN2 protein, human (EC 1.14.11.29) ; EGLN3 protein, human (EC 1.14.11.29) ; Hypoxia-Inducible Factor-Proline Dioxygenases (EC 1.14.11.29)
    Language English
    Publishing date 2021-03-19
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2146365-7
    ISSN 1533-0338 ; 1533-0346
    ISSN (online) 1533-0338
    ISSN 1533-0346
    DOI 10.1177/1533033821990010
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5871: Show issues Location:
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  4. Article ; Online: Dermal scarification versus intramuscular diclofenac sodium injection for the treatment of renal colic: a prospective randomized clinical trial.

    Ioannidis, Stavros / Kampantais, Spyridon / Ioannidis, Anastasios / Gkagkalidis, Konstantinos / Vakalopoulos, Ioannis / Toutziaris, Chrysovalantis / Patsialas, Christos / Laskaridis, Leonidas / Dimopoulos, Panagiotis / Dimitriadis, Georgios

    Urolithiasis

    2014  Volume 42, Issue 6, Page(s) 527–532

    Abstract: The aim of the study is to determine whether dermal scarification is equally effective for treating acute renal colic compared to diclofenac sodium intramuscular therapy. A prospective, randomized controlled study was conducted with methodologic rigor ... ...

    Abstract The aim of the study is to determine whether dermal scarification is equally effective for treating acute renal colic compared to diclofenac sodium intramuscular therapy. A prospective, randomized controlled study was conducted with methodologic rigor based on CONSORT criteria. A total of 291 patients, aged ≥ 18 years, suffering from acute renal colic were included in this trial and randomly assigned in two groups. Patients in the first group (A) received endodermal injection (dermal scarification) of 1 ml normal saline at the area of intensity of pain. The second group (B) received 75 mg diclofenac sodium by intramuscular injection. The success of each method defined the primary end point. Pain intensity before and after treatment was assessed using a visual analog scale. The time onset and the duration of analgesia were also recorded. There was no significant difference between the two groups regarding hematuria (p = 0.158), stone identification at KUB (p = 0.751) and mean pain intensity (p = 0.609) before treatment initiation. The method was successful in 75.5 % of patients in group A and 74.3 % of patients in group B (p = 0.812). Mean pain reduction was comparable, 5.65 ± 3.05 in group A and 5.34 ± 2.99 in group B (p = 0.379), with dermal scarification eliciting its effect considerably faster, whereas the duration of analgesia was longer in the diclofenac group (p < 0.05). In conclusion, dermal scarification could constitute an alternative method for treating renal colic as it is equally effective compared to the standard treatment of diclofenac sodium.
    MeSH term(s) Adult ; Anti-Inflammatory Agents, Non-Steroidal/administration & dosage ; Anti-Inflammatory Agents, Non-Steroidal/therapeutic use ; Complementary Therapies ; Diclofenac/administration & dosage ; Diclofenac/therapeutic use ; Female ; Humans ; Injections, Intradermal ; Injections, Intramuscular ; Male ; Middle Aged ; Pain/drug therapy ; Pain Measurement ; Prospective Studies ; Renal Colic/drug therapy ; Sodium Chloride/administration & dosage ; Sodium Chloride/therapeutic use ; Treatment Outcome
    Chemical Substances Anti-Inflammatory Agents, Non-Steroidal ; Diclofenac (144O8QL0L1) ; Sodium Chloride (451W47IQ8X)
    Language English
    Publishing date 2014-07-30
    Publishing country Germany
    Document type Journal Article ; Randomized Controlled Trial
    ZDB-ID 2703553-0
    ISSN 2194-7236 ; 2194-7228
    ISSN (online) 2194-7236
    ISSN 2194-7228
    DOI 10.1007/s00240-014-0690-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 928: Show issues Location:
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  5. Article: Papillary cystadenoma of epididymis: Is there a need for further investigation in unilateral cases?

    Toutziaris, Chrysovalantis / Kampantais, Spyridon / Perdikis, Ioannis / Gourvas, Victoras / Laskaridis, Leonidas / Gkagkalidis, Konstantinos / Lakis, Sotiris / Ioannidis, Stavros

    International journal of surgery case reports

    2013  Volume 4, Issue 7, Page(s) 616–618

    Abstract: Introduction: The presence of a mass in the epididymis is not a common entity. The papillary cystadenoma of epididymis is a benign tumor which may occur sporadically or as a characteristic of von Hippel-Lindau disease.: Presentation of case: We ... ...

    Abstract Introduction: The presence of a mass in the epididymis is not a common entity. The papillary cystadenoma of epididymis is a benign tumor which may occur sporadically or as a characteristic of von Hippel-Lindau disease.
    Presentation of case: We present a case of a 27-year-old man with a right scrotal mass who was treated with surgical excision. Histopathological examination revealed a clear cell epididymal papillary cystadenoma. A computed tomography scan that was performed later showed no other abnormality or any signs of von Hippel-Lindau disease.
    Discussion: In this report, a case of a young man suffering from this rare tumor is discussed, focusing on the need of further evaluation in order to determinate if it occurs as a feature of VHL disease or as a sporadic form.
    Conclusion: In unilateral cases of papillary cystadenoma of epididymis such as our patient's, literature advocates that no further examinations and expensive genetic testing is required.
    Language English
    Publishing date 2013-04-17
    Publishing country Netherlands
    Document type Journal Article
    ISSN 2210-2612
    ISSN 2210-2612
    DOI 10.1016/j.ijscr.2013.03.038
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Pneumoscrotum as complication of blunt thoracic trauma: a case report.

    Lostoridis, Eftychios / Gkagkalidis, Konstantinos / Varsamis, Nikolaos / Salveridis, Nikolaos / Karageorgiou, Georgios / Kampantais, Spyridon / Tourountzi, Paraskevi / Pouggouras, Konstantinos

    Case reports in surgery

    2013  Volume 2013, Page(s) 392869

    Abstract: Introduction. Pneumoscrotum is a rare clinical entity. It presents with swollen scrotal sac and sometimes with palpable crepitus. It has many etiologies. One of them is due to blunt trauma of the thoracic cage, causing pneumothorax and/or ... ...

    Abstract Introduction. Pneumoscrotum is a rare clinical entity. It presents with swollen scrotal sac and sometimes with palpable crepitus. It has many etiologies. One of them is due to blunt trauma of the thoracic cage, causing pneumothorax and/or pneumomediastinum. Case Presentation. We report the case of an 82-year-old male who was transferred to the Emergency Department with signs of respiratory distress after a blunt chest trauma. A CT scan was obtained, and bilateral pneumothoraces with four broken ribs were disclosed. Subcutaneous emphysema expanding from the eyelids to the scrotum was observed, and a chest tube was inserted on the right side with immediate improvement of the vital signs of the patient. Discussion. Pneumoscrotum has three major etiologies: (a) local introduction of air or infection from gas-producing bacteria, (b) pneumoperitoneum, and (c) air accumulation from lungs, mediastinum, or retroperitoneum. These sources account for most of the cases described in the literature. Treatment should be individualized, and surgical consultation should be obtained in all cases. Conclusion. Although pneumoscrotum itself is a benign entity, the process by which air accumulates in the scrotum must be clarified, and treatment must target the primary cause.
    Language English
    Publishing date 2013-01-14
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2657697-1
    ISSN 2090-6919 ; 2090-6900
    ISSN (online) 2090-6919
    ISSN 2090-6900
    DOI 10.1155/2013/392869
    Database MEDical Literature Analysis and Retrieval System OnLINE

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