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  1. Article ; Online: Improved detection of esp, hyl, asa1, gelE, cylA virulence genes among clinical isolates of Enterococci.

    Kiruthiga, Alexander / Padmavathy, Kesavaram / Shabana, Praveen / Naveenkumar, Venkatesan / Gnanadesikan, Sumathi / Malaiyan, Jeevan

    BMC research notes

    2020  Volume 13, Issue 1, Page(s) 170

    Abstract: Objective: Virulence factors (VFs) among the clinical strains of enterococci play a vital role in pathogenesis. This study was aimed to screen for cylA, asa1, gelE, esp and hyl among Enterococcus faecalis (n = 89) and E. faecium (n = 51) by multiplex ... ...

    Abstract Objective: Virulence factors (VFs) among the clinical strains of enterococci play a vital role in pathogenesis. This study was aimed to screen for cylA, asa1, gelE, esp and hyl among Enterococcus faecalis (n = 89) and E. faecium (n = 51) by multiplex PCR. The previously reported multiplex PCR was modified to 2 duplex (asa1 and gelE, cylA and esp) PCRs and 1 simplex (hyl) PCR. The idea of the modification of the multiplex PCR proposed here emerged in the course of the research study when majority of the isolates which phenotypically exhibited virulence traits were found to be negative for the respective gene.
    Results: cylA, gelE and asa1 were significantly predominant in E. faecalis (59.55%, 85.39%, 86.51%) than E. faecium (1.96%, 60.78%, 9.80%) (p < 0.0001, p = 0.001967, p < 0.0001). hyl was detected in E. faecium (5.9%) only. The number of VFs detected in each isolate was recorded as the VF score. E. faecalis isolates had a VF score pattern of score 4 (34.83%), score 3 (26.96%), score 2 (28.08%) and score 1 (8.98%) while E. faecium had score 4 (1.96%), score 3 (7.84%), score 2 (25.49%) and score 1 (41.18%). This modification of the PCR protocol could resolve the problem of decreased detection of virulence determinants in enterococci.
    MeSH term(s) Bacterial Proteins/isolation & purification ; Enterococcus faecalis/isolation & purification ; Enterococcus faecalis/pathogenicity ; Enterococcus faecium/isolation & purification ; Enterococcus faecium/pathogenicity ; Genes, Bacterial ; Humans ; India ; Membrane Proteins/isolation & purification ; Multiplex Polymerase Chain Reaction ; Virulence/genetics
    Chemical Substances Bacterial Proteins ; Membrane Proteins ; aggregation substance, Enterococcus faecalis ; enterococcal surface protein, esp
    Language English
    Publishing date 2020-03-20
    Publishing country England
    Document type Journal Article
    ZDB-ID 2413336-X
    ISSN 1756-0500 ; 1756-0500
    ISSN (online) 1756-0500
    ISSN 1756-0500
    DOI 10.1186/s13104-020-05018-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: A case report of strongyloidiasis associated with giardiasis in a patient with renal calculi from a tertiary care center in South India.

    Nasimuddin, Sowmya / Malayan, Jeevan / Gnanadesikan, Sumathi / Kandaswamy, Mohanakrishnan

    Journal of global infectious diseases

    2014  Volume 6, Issue 3, Page(s) 137

    Language English
    Publishing date 2014-06-28
    Publishing country India
    Document type Journal Article
    ZDB-ID 2545454-7
    ISSN 0974-8245 ; 0974-777X
    ISSN (online) 0974-8245
    ISSN 0974-777X
    DOI 10.4103/0974-777X.138516
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Clinical profile and atypical manifestation of

    Mohan, Kamalraj / Malaiyan, Jeevan / Nasimuddin, Sowmya / Devasir, Ravin Sathyaseelan / Meenakshi-Sundaram, PradeepRaj / Selvaraj, Santhiya / Krishnasamy, Bharathi / Gnanadesikan, Sumathi / Karthikeyan, Muthulakshmi / Kandasamy, Mohanakrishnan / Jayakumar, Nithyalakshmi / Elumalai, Dhevahi / Ra, Gokul G

    Journal of family medicine and primary care

    2020  Volume 9, Issue 2, Page(s) 1119–1123

    Abstract: Introduction: Dengue fever is a common mosquito-borne viral disease which has reached alarming size in the past few years. It is endemic in more than 100 countries and significant differences were found in clinical profile and atypical manifestation.: ...

    Abstract Introduction: Dengue fever is a common mosquito-borne viral disease which has reached alarming size in the past few years. It is endemic in more than 100 countries and significant differences were found in clinical profile and atypical manifestation.
    Aim of the study: A retrospective observational study of clinical profile and atypical manifestations in patients with dengue fever.
    Materials and methods: Serum samples were collected from clinically suspected cases of dengue fever and it was confirmed by NS1 antigen, IgM, and IgG antibody by ELISA. Clinical details and atypical manifestations were recorded.
    Observation: During the study period, a total of 2502 patients were suspected to have dengue infection, of which 464 (18.5%) samples were found to be positive for dengue viral infection. A majority of the cases were males [268 (57%)] when compared with females [196 (42%)]. Fever was the most common clinical presentation seen in all the patients, followed by headache (78.4%), myalgia (61%), body pain (49.3%), vomiting (40%), joint pain (31.5%), dry cough (19%), nausea (14%), abdominal pain (8%), diarrhea (5.6%), retro-orbital pain (04%), burning micturition (2.4%), and rashes (0.6%). Among atypical manifestations, hepatomegaly [32 (7%)] was the most common, followed by splenomegaly [23 (5%)], bradycardia [18 (4%)], meningitis [6 (1.2%)], hemoptysis [5 (1%)], acalculous cholecystitis [4 (0.8%)], and acute pancreatitis [2 (0.4%)]. The study of hematological parameters showed thrombocytopenia was present in 179 (38.5%) patients, followed by leukopenia [77 (17%)] and raised hematocrit [29 (6.2%)].
    Conclusion: During ongoing epidemics, the clinical profile and atypical manifestations in clinically suspected dengue patients should be investigated early so that severe forms can be treated promptly.
    Language English
    Publishing date 2020-02-28
    Publishing country India
    Document type Journal Article
    ZDB-ID 2735275-4
    ISSN 2278-7135 ; 2249-4863
    ISSN (online) 2278-7135
    ISSN 2249-4863
    DOI 10.4103/jfmpc.jfmpc_926_19
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Novel gas producing

    Malaiyan, Jeevan / Balakrishnan, Anandan / Nasimuddin, Sowmya / Mohan, Kamalraj / Meenakshi-Sundaram, PradeepRaj / Mamandur-Devarajan, Selvam / Gnanadesikan, Sumathi / Kandasamy, Mohanakrishnan / Jayakumar, Nithyalakshmi / Elumalai, Dhevahi / Ra, Gokul G

    Access microbiology

    2019  Volume 1, Issue 2, Page(s) e000005

    Abstract: Background: Bacterial characterization is important in clinical and epidemiological studies. We herein report the first case of gas-producing : Case presentation: A 30-year-old female presented to the emergency department with complaints of about ten ...

    Abstract Background: Bacterial characterization is important in clinical and epidemiological studies. We herein report the first case of gas-producing
    Case presentation: A 30-year-old female presented to the emergency department with complaints of about ten episodes of watery diarrhea, four episodes of vomiting and elevated serum urea/creatinine levels. Although the bacteria were first misidentified as
    Conclusions: Literature survey from the PubMed database shows no gas-producing
    Language English
    Publishing date 2019-04-17
    Publishing country England
    Document type Case Reports
    ISSN 2516-8290
    ISSN (online) 2516-8290
    DOI 10.1099/acmi.0.000005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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