Article ; Online: Structure of the essential diversity-generating retroelement protein bAvd and its functionally important interaction with reverse transcriptase.
Structure (London, England : 1993)
2012 Volume 21, Issue 2, Page(s) 266–276
Abstract: Diversity-generating retroelements (DGRs) are the only known source of massive protein sequence variation in prokaryotes. These elements transfer coding information from a template region (TR) through an RNA intermediate to a protein-encoding variable ... ...
| Abstract | Diversity-generating retroelements (DGRs) are the only known source of massive protein sequence variation in prokaryotes. These elements transfer coding information from a template region (TR) through an RNA intermediate to a protein-encoding variable region. This retrohoming process is accompanied by unique adenine-specific mutagenesis and, in the prototypical BPP-1 DGR, requires a reverse transcriptase (bRT) and an accessory variability determinant (bAvd) protein. To understand the role of bAvd, we determined its 2.69 Å resolution structure, which revealed a highly positively charged pentameric barrel. In accordance with its charge, bAvd bound both DNA and RNA, albeit without a discernable sequence preference. We found that the coding sequence of bAvd functioned as part of TR but identified means to mutate bAvd without affecting TR. This mutational analysis revealed a strict correspondence between retrohoming and interaction of bAvd with bRT, suggesting that the bRT-bAvd complex is important for DGR retrohoming. |
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| MeSH term(s) | Amino Acid Substitution ; Bacteriophages/enzymology ; Bordetella/virology ; Crystallography, X-Ray ; DNA, Single-Stranded/chemistry ; Models, Molecular ; Mutagenesis, Site-Directed ; Protein Binding ; Protein Structure, Quaternary ; Protein Structure, Secondary ; RNA/chemistry ; RNA-Directed DNA Polymerase/chemistry ; Retroelements ; Viral Proteins/chemistry ; Viral Proteins/genetics |
| Chemical Substances | DNA, Single-Stranded ; Retroelements ; Viral Proteins ; RNA (63231-63-0) ; RNA-Directed DNA Polymerase (EC 2.7.7.49) |
| Language | English |
| Publishing date | 2012-12-27 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, N.I.H., Extramural |
| ZDB-ID | 1213087-4 |
| ISSN | 1878-4186 ; 0969-2126 |
| ISSN (online) | 1878-4186 |
| ISSN | 0969-2126 |
| DOI | 10.1016/j.str.2012.11.016 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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