Article ; Online: Validation of qPCR reference genes in the endangered annual killifish Austrolebias charrua considering different tissues, gender and environmental conditions.
Ecotoxicology (London, England)
2024
Abstract: The annual killifish Austrolebias charrua is an endangered species, endemic to the southern region of South America, which inhabits temporary ponds that emerges in the rainy season. The main anthropogenic threat driving the extinction of A. charrua stems ...
| Abstract | The annual killifish Austrolebias charrua is an endangered species, endemic to the southern region of South America, which inhabits temporary ponds that emerges in the rainy season. The main anthropogenic threat driving the extinction of A. charrua stems from extensive agriculture, primarily due to the widrespread use of glyphosate-based herbicides near their habitats. Annual killifishes have been used as models for ecotoxicological studies but, up to now, there are no studies about reference genes in any Austrolebias species. This represents an obstacle to the use of qPCR-based technologies, the standard method for gene expression quantification. The present study aimed to select and validate potential reference genes for qPCR normalization in the annual killifish Austrolebias charrua considering different tissues, gender and environmental conditions. The candidate reference genes 18 s, actb, gapdh, ef1a, shox, eif3g, and the control gene atp1a1 were evaluated in male and female individuals in three different tissues (brain, liver, and gills) under two experimental conditions (control and acute exposition to Roundup Transorb |
|---|---|
| Language | English |
| Publishing date | 2024-04-11 |
| Publishing country | United States |
| Document type | Journal Article |
| ZDB-ID | 34042-x |
| ISSN | 1573-3017 ; 0963-9292 |
| ISSN (online) | 1573-3017 |
| ISSN | 0963-9292 |
| DOI | 10.1007/s10646-024-02752-0 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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