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Article ; Online: The Effects of Ying Yang Bao on Nutritional Status of Children Aged 6-60 Months in Underdeveloped Rural Areas of China.

Feng, Jing / Wang, Yongjun / Liu, Tingting / Huo, Junsheng / Zhuo, Qin / Gong, Zhaolong

2024 Volume 16, Issue 2

Abstract: The Ying Yang Bao (YYB) intervention, a national policy in China, has been implemented for over two decades. Most previous studies have focused only on the short-term effects of YYB, while the long-term effects remain unexplored. This study was designed ... ...

Abstract	The Ying Yang Bao (YYB) intervention, a national policy in China, has been implemented for over two decades. Most previous studies have focused only on the short-term effects of YYB, while the long-term effects remain unexplored. This study was designed to evaluate the long-term effects of YYB in children aged 6-60 months. A sample of 4666 children was divided into intervention and control groups. Information on basic characteristics, physical examination, YYB consumption, etc., was obtained annually from 2018 to 2021. T-tests or chi-square tests were used to compare differences between the groups for continuous or categorical variables. Children in the intervention group showed greater incremental improvements in hemoglobin levels and physical development (
MeSH term(s)	Humans ; Anemia/epidemiology ; Cachexia ; China/epidemiology ; Growth Disorders ; Nutritional Status ; Infant ; Child, Preschool
Language	English
Publishing date	2024-01-08
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2518386-2
ISSN	2072-6643 ; 2072-6643
ISSN (online)	2072-6643
ISSN	2072-6643
DOI	10.3390/nu16020202
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Complementary Feeding and Malnutrition among Infants and Young Children Aged 6-23 Months in Rural Areas of China.

Feng, Jing / Gong, Zhaolong / Wang, Yongjun / Huo, Junsheng / Zhuo, Qin

Nutrients

2022 Volume 14, Issue 9

Abstract: This study investigated the nutrition and complementary feeding (CF) of infants and young children (IYC) aged 6-23 months in rural areas of China in 2018 and explored the relationship between CF and nutritional status. We measured the length and weight, ... ...

Abstract	This study investigated the nutrition and complementary feeding (CF) of infants and young children (IYC) aged 6-23 months in rural areas of China in 2018 and explored the relationship between CF and nutritional status. We measured the length and weight, calculated the z-scores, and detected micronutrients in the hair. The status of CF was obtained from the respondents by a 24-h dietary recall. IYC were classified into clusters using a two-step cluster analysis. The CF and nutritional status of each cluster were analyzed and compared. The prevalence of stunting, wasting, and overweight in the IYC in rural Chinese areas was 7.1%, 3.0%, and 3.7%, respectively. The median levels of Ca, Fe, and Zn in hair were 550.10 µg/g, 62.94 µg/g, and 132.86 µg/g, respectively. The prevalence of meeting the requirements of minimum dietary diversity (MDD), minimum meal frequency (MMF), and minimum acceptable diet (MAD) was 68.9%, 77.9%, and 46.4%, respectively. IYC with a higher prevalence of MDD, MMF, and MAD were more inclined to maintain a healthy status. The prevalence of undernutrition and overweight of 6- to 23-month-old IYC in rural areas of China was low. However, lack of trace elements was evident, and MAD prevalence remained low.
MeSH term(s)	Breast Feeding ; Child ; Child, Preschool ; China/epidemiology ; Female ; Humans ; Infant ; Infant Nutritional Physiological Phenomena ; Malnutrition/epidemiology ; Nutritional Status ; Overweight/epidemiology ; Socioeconomic Factors
Language	English
Publishing date	2022-04-26
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2518386-2
ISSN	2072-6643 ; 2072-6643
ISSN (online)	2072-6643
ISSN	2072-6643
DOI	10.3390/nu14091807
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Article: [Determination of vitamin A and vitamin E in human serum by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry].

Chen, Xi / Gong, Zhaolong / Shen, Shi

Wei sheng yan jiu = Journal of hygiene research

2021 Volume 50, Issue 2, Page(s) 301–307

Abstract: Objective: To establish a method for the determination of vitamin A(retinol) and four active forms of vitamin E(α-tocopherol, β-tocopherol, γ-tocopherol and δ-tocopherol) in human serum by ultra-high performance liquid chromatography-tandem triple ... ...

Abstract	Objective: To establish a method for the determination of vitamin A(retinol) and four active forms of vitamin E(α-tocopherol, β-tocopherol, γ-tocopherol and δ-tocopherol) in human serum by ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometry(UPLC-MS/MS). Methods: The sample was deproteinized by methanol, then extracted by n-hexane, dryness under nitrogen and followed by a reconstitution with methanol. The analysis was performed on a C_(30) column(3 mm×150 mm, 2. 6 μm), and isometric elution using 0. 1% formic acid in methanol and 5 mmol/L ammonium formate in 0. 1% formic acid as the mobile phase. The samples were determined by mass spectrometry in the positive ion mode with the multiple reaction monitoring mode, quantified by the internal standard method. Results: Vitamin A and four active forms of vitamin E were separated within 42 minutes, and β-tocopherol and γ-tocopherol can be distinguished. The linear was good for retinol in the range of 0. 0050-2. 5 μg/mL, 0. 036-20 μg/mL for α-tocopherol, 0. 042-8. 0 μg/mL for β-tocopherol and 0. 020-10 μg/mL for the other tocopherols. The limits of detection for retinol and tocopherols were in the range of 5. 76-31. 6 ng/mL. Recoveries of retinol and tocopherols at different levels were in range of 84. 4%-118. 6%, with the relative standard deviations were 1. 22%-8. 50%(n=6). Conclusion: This method is fast, accurate and sensitive and the preprocessing is simple, which can be used for determination of vitamin A and four active forms of vitamin E in human serum effectively.
MeSH term(s)	Chromatography, High Pressure Liquid ; Chromatography, Liquid ; Humans ; Tandem Mass Spectrometry ; Vitamin A ; Vitamin E
Chemical Substances	Vitamin A (11103-57-4) ; Vitamin E (1406-18-4)
Language	Chinese
Publishing date	2021-05-13
Publishing country	China
Document type	Journal Article
ZDB-ID	1050909-4
ISSN	1000-8020
ISSN	1000-8020
DOI	10.19813/j.cnki.weishengyanjiu.2021.02.023
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ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Identification and function analysis of GABA branch three gene families in the cotton related to abiotic stresses.

Zheng, Juyun / Zhang, Zeliang / Zhang, Nala / Liang, Yajun / Gong, Zhaolong / Wang, Junhao / Ditta, Allah / Sang, Zhiwei / Wang, Junduo / Li, Xueyuan

BMC plant biology

2024 Volume 24, Issue 1, Page(s) 57

Abstract: γ -aminobutyric acid (GABA) is closely related to the growth, development and stress resistance of plants. Combined with the previous study of GABA to promote the cotton against abiotic stresses, the characteristics and expression patterns of GABA branch ...

Abstract	γ -aminobutyric acid (GABA) is closely related to the growth, development and stress resistance of plants. Combined with the previous study of GABA to promote the cotton against abiotic stresses, the characteristics and expression patterns of GABA branch gene family laid the foundation for further explaining its role in cotton stress mechanism. Members of GAD, GAB-T and SSADH (three gene families of GABA branch) were identified from the Gossypium hirsutum, Gossypium barbadense, Gossypium arboreum and Gossypium raimondii genome. The GABA branch genes were 10 GAD genes, 4 GABA-T genes and 2 SSADH genes. The promoter sequences of genes mainly contains response-related elements such as light, hormone and environment.Phylogenetic analysis shows that GAD indicating that even in the same species, the homologous sequences in the family. The GABA-T gene of each cotton genus was in sum the family had gene loss in the process of dicotyledon evolution. SSADH families Gossypium hirsutum, Gossypium barbadense, Gossypium arboreum and Gossypium raimondii were closely related to the dicot plants.GABA gene is involved in the regulation of salt stress and high temperature in Gossypium hirsutum.GABA attenuated part of the abiotic stress damage by increasing leaf protective enzyme activity and reducing reactive oxygen species production.This lays the foundation for a thorough analysis of the mechanism of GABA in cotton stress resistance.
MeSH term(s)	Gossypium/metabolism ; Multigene Family ; Phylogeny ; Genes, Plant/genetics ; Stress, Physiological/genetics ; Gene Expression Regulation, Plant ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Genome, Plant
Chemical Substances	Plant Proteins
Language	English
Publishing date	2024-01-19
Publishing country	England
Document type	Journal Article
ZDB-ID	2059868-3
ISSN	1471-2229 ; 1471-2229
ISSN (online)	1471-2229
ISSN	1471-2229
DOI	10.1186/s12870-024-04738-w
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Article: [Blood cadmium level and the change of cadmium exposure risk in Chinese urban school-age children in 2002 and 2012].

Liu, Xiaobing / Zhang, Yu / Gong, Zhaolong / Zhang, Huidi / Wang, Jun / Tan, Hongxing / Piao, Jianhua / Yang, Lichen / Yang, Xiaoguang

Wei sheng yan jiu = Journal of hygiene research

2024 Volume 53, Issue 2, Page(s) 202–208

Abstract: Objective: To investigate the blood cadmium concentrations and the related change in Chinese urban children derived from the China Nutrition and Health Survey 2002 and 2012(CNHS 2002 and CNHS 2012).: Methods: The Chinese urban children aged 6-11 ... ...

Abstract	Objective: To investigate the blood cadmium concentrations and the related change in Chinese urban children derived from the China Nutrition and Health Survey 2002 and 2012(CNHS 2002 and CNHS 2012). Methods: The Chinese urban children aged 6-11 years were selected according to gender, age and regional distribution using the multi-stage stratified cluster random sampling method, as well as the corresponding whole blood samples. The blood cadmium concentration was carefully determined by the quadrupole inductively coupled plasma mass spectrometry(ICP-MS) and the percentage of blood cadmium over 2 μg/L was subsequently estimated. In addition, the upper limit values of the 95%CI of the 95th percentiles of available blood cadmium data was assessed as the threshold of cadmium exposure. Results: Totally, 2182 Chinese urban children were included, and of these, 1036 children were from the CNHS 2002 and 1146 children were from the CNHS 2012. From the CNHS 2002 to the CNHS 2012, the median blood cadmium concentration was increased from 0.28 μg/L to 0.95 μg/L, and the percentage of blood cadmium with over 2 μg/L was elevated from 1.45% to 10.47%. In addition, the new estimated threshold of blood cadmium was ascended from 1.24 μg/L up to 2.89 μg/L. Conclusion: The risk of cadmium exposure in Chinese urban children aged 6-11 years was increasingly aggravated from the CHNS 2002 to the CNHS 2012.
MeSH term(s)	Child ; Humans ; Cadmium/blood ; China ; East Asian People ; Environmental Exposure
Chemical Substances	Cadmium (00BH33GNGH)
Language	Chinese
Publishing date	2024-04-11
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	1050909-4
ISSN	1000-8020
ISSN	1000-8020
DOI	10.19813/j.cnki.weishengyanjiu.2024.02.005
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bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Effects of the Duration of Ying Yang Bao Consumption on Hemoglobin Concentration in Infants and Young Children in Less Developed Areas of China.

Feng, Jing / Wang, Yongjun / Liu, Tingting / Huo, Junsheng / Zhuo, Qin / Gong, Zhaolong

Nutrients

2022 Volume 14, Issue 21

Abstract: Ying Yang Bao (YYB) is conventionally prescribed as a nutritional supplement to infants and young children (IYC) in less developed areas of China. However, whether 18-month YYB consumption is reasonable needs assessment. This study examined the influence ...

Abstract	Ying Yang Bao (YYB) is conventionally prescribed as a nutritional supplement to infants and young children (IYC) in less developed areas of China. However, whether 18-month YYB consumption is reasonable needs assessment. This study examined the influence of the duration of YYB consumption on hemoglobin (Hb) levels and anemia prevalence. Data from the Nutrition Improvement Project on Children in Poor Areas of China in 2018-2019 were used. Questionnaires were used to collect information on basic characteristics, dietary status, and YYB consumption. Propensity score matching (PSM) was used to balance confounders. Hb levels and anemia prevalence in IYC with different durations of YYB consumption were compared. After PSM, all covariates were well-balanced, and 1151 pairs of IYC were included in subsequent analyses. During the 1st-9th months of intervention, YYB effectively increased Hb levels and reduced anemia prevalence in the intervention group. During the 10th-18th months of intervention, Hb levels in the control group increased and anemia prevalence decreased, while Hb levels and anemia prevalence fluctuated in the intervention group. In conclusion, YYB was effective in improving nutritional status of infants, but had a limited effect in young children. Nutritional supplements with different quantities or nutrients should be considered for young children.
MeSH term(s)	Infant ; Child ; Humans ; Child, Preschool ; Rural Population ; China/epidemiology ; Anemia/epidemiology ; Anemia/prevention & control ; Dietary Supplements ; Prevalence ; Hemoglobins/analysis
Chemical Substances	Hemoglobins
Language	English
Publishing date	2022-10-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2518386-2
ISSN	2072-6643 ; 2072-6643
ISSN (online)	2072-6643
ISSN	2072-6643
DOI	10.3390/nu14214539
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Article: [Optimization of three-dimensional culture conditions of L02 cells with response surface methodology based on VitroGel system].

Wang, Jingbo / Chen, Chen / Ma, Yan / Yang, Zhuo / Qin, Wen / Wang, Liyuan / Shen, Shi / Gong, Zhaolong / Zhuo, Qin

Wei sheng yan jiu = Journal of hygiene research

2023 Volume 52, Issue 4, Page(s) 579–610

Abstract: Objective: This study optimizes three-dimensional(3D)culture conditions of L02 cells using response surface methodology(RSM) based on the VitroGel system to construct the hepatocytes model in vitro.: Methods: L02 cells were 3D cultured by the ... ...

Abstract	Objective: This study optimizes three-dimensional(3D)culture conditions of L02 cells using response surface methodology(RSM) based on the VitroGel system to construct the hepatocytes model in vitro. Methods: L02 cells were 3D cultured by the VitroGel system. The appropriate level of three key factors(concentration of inoculated cells, culture time and dilution degree of the hydrogel) was determined by single-factor experiment, and the optimal conditions of 3D culture of L02 cells based on the VitroGel system were determined by RSM. During the detection process, the optical density(OD) value of cell viability was used as the detection index, and the cell viability was detected using the cell counting kit-8(CCK-8) assay. The proliferative performance and viability of L02 cells was measured by fluorescent staining assay. Results: The selected optimal culture conditions by RSM were as follows: concentration of inoculated cells was 1.1 × 10~5/mL, culture time was 9.5 days, and dilution degree of hydrogel was 1∶3.7. The result shows that under optimal conditions, the predicted OD value of cell viability was 2.17 and measured 2.13 with a relative error of 1.84%, indicating that the condition was suitable and reliable. The fluorescent staining and dead and live cells detection results showed the 3D hepatocytes model was successfully constructed. Conclusion: The optimal conditions for 3D culture of L02 cell based on the VitroGel system were determined by RSM, and a hepatocytes model with high cellular activity was successfully constructed.
MeSH term(s)	Cell Survival ; Hepatocytes ; Hydrogels ; Indicator Dilution Techniques
Chemical Substances	Hydrogels
Language	Chinese
Publishing date	2023-08-28
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	1050909-4
ISSN	1000-8020
ISSN	1000-8020
DOI	10.19813/j.cnki.weishengyanjiu.2023.04.010
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Zs.A 4878: Show issues

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Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

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Article: [Serum metabolomics of iron deficiency anemia in infants based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry].

Shen, Shi / Sun, Jing / Yin, Jiyong / Liu, Tingting / Chen, Xi / Gong, Zhaolong / Zhuo, Qin / Huo, Junsheng

Wei sheng yan jiu = Journal of hygiene research

2023 Volume 52, Issue 1, Page(s) 123–128

Abstract: Objective: To explore the differential metabolites in the serum of infants with iron deficiency anemia(IDA) and non iron deficiency anemia, and to explore the potential biomarkers.: Methods: Non-targeted metabolomics of 30 infants with iron ... ...

Abstract	Objective: To explore the differential metabolites in the serum of infants with iron deficiency anemia(IDA) and non iron deficiency anemia, and to explore the potential biomarkers. Methods: Non-targeted metabolomics of 30 infants with iron deficiency anemia aged 6-11 months and 30 infants with non iron deficiency anemia aged 6-11 months were analyzed by ultra-high performance liquid chromatography with time of flight mass spectrometry using Acquity UPLC BEH C_(18) column(100 mm×2.1 mm, 1.8 μm). The differences of metabolites between the two groups were analyzed by principal component analysis(PCA) and orthogonal partial least squares discriminant analysis(OPLS-DA). Differential metabolites were screened according to OPLS-DA variable importance projection(VIP) >1. The related metabolic pathways involved in the markers were analyzed based on the KEGG database. Results: Differences in serum metabolic profiles between iron deficiency anemia group and non iron deficiency anemia group were observed. The 44 potential biomarkers were mainly lipids. Combined with pathway analysis, the metabolic pathways related to different metabolites included glycerophosphingolipid metabolism and sphingolipid metabolism. Conclusion: There are differences in lipid metabolites between infants with non iron deficiency anemia and iron deficiency anemia, suggesting that the occurrence and progress of iron deficiency anemia are related to lipid metabolism.
MeSH term(s)	Humans ; Infant ; Metabolomics/methods ; Chromatography, Liquid ; Chromatography, High Pressure Liquid/methods ; Mass Spectrometry/methods ; Biomarkers/metabolism ; Anemia
Chemical Substances	Biomarkers
Language	Chinese
Publishing date	2023-02-07
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	1050909-4
ISSN	1000-8020
ISSN	1000-8020
DOI	10.19813/j.cnki.weishengyanjiu.2023.01.021
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Zs.A 4878: Show issues

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Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Trajectories of 24-Hour Physical Activity Distribution and Relationship with Dyslipidemia.

Wang, Xiaojing / Wang, Yongjun / Xu, Zechao / Guo, Xiang / Mao, Hongmei / Liu, Tingting / Gong, Weiyi / Gong, Zhaolong / Zhuo, Qin

Nutrients

2023 Volume 15, Issue 2

Abstract: The association between physical activity (PA) and dyslipidemia is well known, but the relationship between a temporal pattern of PA and dyslipidemia remain unknown. Here, we aimed to identify the intensity and temporal patterns of PA clustered by the ... ...

Abstract	The association between physical activity (PA) and dyslipidemia is well known, but the relationship between a temporal pattern of PA and dyslipidemia remain unknown. Here, we aimed to identify the intensity and temporal patterns of PA clustered by the trajectory model and their relationship with dyslipidemia. The participants were 701 adults (305 males) aged 18−60 years undergoing continuous measurement of PA with Actigraph GT3X+ accelerometers for at least 3 days. A trajectory analysis was applied based on moderate-to-vigorous intensity physical activity (MVPA) accumulated values over every period per day. The association between PA and dyslipidemia was estimated using a logistic regression model. Four distinct PA trajectory groups in the population were identified (continued low, stable and moderate, late increasing, and early increasing). Specifically, the “moderate and stable group” was associated with a decreased rate of high TG (p < 0.05) and the “moderate and stable group” and “late increasing group” were associated with decreased rates of low HDL-C (p < 0.05). In conclusion, there were four activity trajectory groups in this population and the continued low PA trajectory was associated with a high prevalent rate of an abnormal lipid profile, and continued and moderate activity or late afternoon increasing activity might have lower HDL-C distribution.
MeSH term(s)	Male ; Adult ; Humans ; Exercise ; Dyslipidemias/epidemiology ; Accelerometry
Language	English
Publishing date	2023-01-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2518386-2
ISSN	2072-6643 ; 2072-6643
ISSN (online)	2072-6643
ISSN	2072-6643
DOI	10.3390/nu15020328
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Article ; Online: Genome-wide identification and expression reveal the involvement of the FCS-like zinc finger (FLZ) gene family in

Wang, JunDuo / Li, Zhiqiang / Liang, Yajun / Zheng, Juyun / Gong, Zhaolong / Zhou, Guohui / Xu, Yuhui / Li, Xueyuan

PeerJ

2023 Volume 11, Page(s) e14690

Abstract: FCS-like zinc finger (FLZ) is a plant-specific gene family that plays an important regulatory role in plant growth and development and its response to stress. However, studies on the characteristics and functions of cotton FLZ family genes are still ... ...

Abstract	FCS-like zinc finger (FLZ) is a plant-specific gene family that plays an important regulatory role in plant growth and development and its response to stress. However, studies on the characteristics and functions of cotton FLZ family genes are still lacking. This study systematically identified members of the cotton FLZ gene family based on cotton genome data. The cotton FLZ family genes were systematically analyzed by bioinformatics, and their expression patterns in different tissues and under low-temperature stress were analyzed by transcriptome and qRT-PCR. The
MeSH term(s)	Gossypium/genetics ; Genome, Plant/genetics ; Temperature ; Multigene Family/genetics ; Zinc Fingers/genetics
Language	English
Publishing date	2023-01-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2703241-3
ISSN	2167-8359 ; 2167-8359
ISSN (online)	2167-8359
ISSN	2167-8359
DOI	10.7717/peerj.14690
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