Article ; Online: Identification of the neutralizing epitopes of Merkel cell polyomavirus major capsid protein within the BC and EF surface loops.
PloS one
2015 Volume 10, Issue 3, Page(s) e0121751
Abstract: Merkel cell polyomavirus (MCPyV) is the first polyomavirus clearly associated with a human cancer, i.e. the Merkel cell carcinoma (MCC). Polyomaviruses are small naked DNA viruses that induce a robust polyclonal antibody response against the major capsid ...
| Abstract | Merkel cell polyomavirus (MCPyV) is the first polyomavirus clearly associated with a human cancer, i.e. the Merkel cell carcinoma (MCC). Polyomaviruses are small naked DNA viruses that induce a robust polyclonal antibody response against the major capsid protein (VP1). However, the polyomavirus VP1 capsid protein epitopes have not been identified to date. The aim of this study was to identify the neutralizing epitopes of the MCPyV capsid. For this goal, four VP1 mutants were generated by insertional mutagenesis in the BC, DE, EF and HI loops between amino acids 88-89, 150-151, 189-190, and 296-297, respectively. The reactivity of these mutants and wild-type VLPs was then investigated with anti-VP1 monoclonal antibodies and anti-MCPyV positive human sera. The findings together suggest that immunodominant conformational neutralizing epitopes are present at the surface of the MCPyV VLPs and are clustered within BC and EF loops. |
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| MeSH term(s) | Animals ; Antibodies, Monoclonal/immunology ; Antibodies, Neutralizing/immunology ; Antibodies, Viral/immunology ; Capsid Proteins/chemistry ; Capsid Proteins/genetics ; Capsid Proteins/immunology ; Cell Line ; Cross Reactions/immunology ; Epitope Mapping ; Epitopes/immunology ; Female ; Humans ; Immunodominant Epitopes/immunology ; Merkel cell polyomavirus/genetics ; Merkel cell polyomavirus/immunology ; Mice ; Models, Molecular ; Mutation ; Protein Conformation ; Protein Interaction Domains and Motifs/immunology |
| Chemical Substances | Antibodies, Monoclonal ; Antibodies, Neutralizing ; Antibodies, Viral ; Capsid Proteins ; Epitopes ; Immunodominant Epitopes |
| Language | English |
| Publishing date | 2015 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ISSN | 1932-6203 |
| ISSN (online) | 1932-6203 |
| DOI | 10.1371/journal.pone.0121751 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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